CN186363

Overview
NameCN186363
Unique NameCN186363
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length802
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_IGNH4 (Elongation factor 1-alpha OS=Ignicoccus hospitalis (strain KIN4/I / DSM 18386 / JCM 14125) GN=tuf PE=3 SV=1)

HSP 1 Score: 304.679 bits (779), Expect = 3.922e-82
Identity = 139/241 (57.68%), Postives = 189/241 (78.42%), Query Frame = 3
Query:   84 KEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEP--KRPSDKPLRLPLQDV 800
            KEK H+N++VIGHVD GKST  GHL+Y+LG +D++ ++  E+EA +  K SFKYAW+LDKLK ERERG+TID+   KFET KYY T+IDAPGHRDFIKNMITG SQAD A+L++ +  G FEAG+S +GQTREH LLA T+G+ Q+I   NKMDAT P +S+ RY +IV+ +  ++K +G+  +++PFVP+S + GDN+I+RS N+ WYKGPTL++ALDN+  P  ++ +  PLR+P+QDV
Sbjct:    4 KEKPHMNLIVIGHVDHGKSTLVGHLLYELGFVDEKTLKMLEEEAKKRGKESFKYAWLLDKLKEERERGVTIDLTFMKFETPKYYFTIIDAPGHRDFIKNMITGASQADAAILVVSARPGEFEAGMSAEGQTREHILLAKTMGIDQIIVAVNKMDATEPPWSEKRYKQIVETLKKFMKGLGFKVDEIPFVPVSAWTGDNIIKRSENMPWYKGPTLVEALDNLKPPSVEKWAKLPLRIPIQDV 244          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_GIALA (Elongation factor 1-alpha (Fragment) OS=Giardia lamblia GN=TEF1 PE=2 SV=1)

HSP 1 Score: 304.679 bits (779), Expect = 3.922e-82
Identity = 147/222 (66.22%), Postives = 175/222 (78.83%), Query Frame = 3
Query:  138 STTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGY-NPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            ST TGHLIYK GGID+R I+ +EK A EM K SFKYAWVLD+LK ERERGITI+IALWKFET KY  T+IDAPGHRDFIKNMITGTSQAD A+L++ +  G FEAGISKDGQTREHA LA TLG+K MI C NKMD    KYSK RYDEI  E+   LK +G+   E+  ++P SG+ GDN++E+S  + WY+GP L+DA+D +  PKRP+DKPLRLP+QDV
Sbjct:    1 STLTGHLIYKCGGIDQRTIDEYEKRATEMGKGSFKYAWVLDQLKDERERGITINIALWKFETKKYIVTIIDAPGHRDFIKNMITGTSQADVAILVVAAGQGEFEAGISKDGQTREHATLANTLGIKTMIICVNKMDDGQVKYSKERYDEIKGEMMKQLKNIGWKKAEEFDYIPTSGWTGDNIMEKSDKMPWYEGPCLIDAIDGLKAPKRPTDKPLRLPIQDV 222          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_AERPE (Elongation factor 1-alpha OS=Aeropyrum pernix GN=tuf PE=3 SV=1)

HSP 1 Score: 301.212 bits (770), Expect = 4.337e-81
Identity = 134/238 (56.30%), Postives = 183/238 (76.89%), Query Frame = 3
Query:   87 EKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            EK H+N+VVIGHVD GKST  GHL+Y+LG I+++ ++  E++A    K SFK+AW+LDK+K ERERGITID+   KFET KY  T+IDAPGHRDF+KNMITG SQAD A+L++ +  G FEAG+S +GQTREH LLA T+G++Q+I   NKMDA    Y + RY+ +V  +  ++K +GY  +K+PF+P+S ++GDN+IERS N+ WY GPTL++ALD +  P +P DKPLR+P+Q+V
Sbjct:    3 EKPHMNLVVIGHVDHGKSTLVGHLLYRLGYIEEKKLKELEEQAKSRGKESFKFAWILDKMKEERERGITIDLTFMKFETKKYVFTIIDAPGHRDFVKNMITGASQADAAILVVSARKGEFEAGMSTEGQTREHLLLARTMGIEQIIVAVNKMDAPDVNYDQKRYEFVVSVLKKFMKGLGYQVDKIPFIPVSAWKGDNLIERSPNMPWYNGPTLVEALDQLQPPAKPVDKPLRIPVQNV 240          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_THEPD (Elongation factor 1-alpha OS=Thermofilum pendens (strain Hrk 5) GN=tuf PE=3 SV=1)

HSP 1 Score: 284.648 bits (727), Expect = 4.200e-76
Identity = 134/242 (55.37%), Postives = 180/242 (74.38%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERS-TNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            M ++K H+N+VVIGH+D GKST  G L+Y++G +D R+I+++E+EA +M + ++KYAWVLDKLK ERE+GITID+  +KFET KY+ T+IDAPGHRDF+KNMITG SQAD A+L++ +  G FEAGIS  GQTREH  LA T+GV Q++   NKMD  T  YSK RY+EI  ++   L+ VGY  +++PF+P S +EG N+ +R+     WY GP L +A D   EP RP DKPLR+P+QDV
Sbjct:    1 MSEKKPHLNLVVIGHIDHGKSTLMGRLLYEIGAVDPRLIQQYEEEAKKMGRETWKYAWVLDKLKEEREKGITIDLGFYKFETKKYFFTLIDAPGHRDFVKNMITGASQADVALLVVSAKEGEFEAGISPAGQTREHVFLAKTMGVDQLVVAINKMD--TVNYSKERYEEIKNQLIRLLRMVGYKVDEIPFIPTSAWEGVNVSKRTPEKTPWYDGPCLYEAFDFFKEPPRPIDKPLRIPIQDV 240          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_RHYAM (Elongation factor 1-alpha (Fragment) OS=Rhynchosciara americana PE=2 SV=1)

HSP 1 Score: 278.87 bits (712), Expect = 2.305e-74
Identity = 142/201 (70.65%), Postives = 160/201 (79.60%), Query Frame = 3
Query:  234 SFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTL------------LDALDNINEPKRPSDKPLRLPLQDV 800
            SFKYAWVLDKLKAERERGITIDIALWKFET KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+GQTREHALLAFTLGVKQ+I   NKMD+T P +S+ RY+EI KEVSSY+KK+GYNP  V FVPISG+ GDNM+E S+N+ W KG  +              ALD I  P RP+D+ LRLPL DV
Sbjct:    2 SFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPFSENRYEEIKKEVSSYIKKIGYNPVAVAFVPISGWHGDNMLEPSSNMRWLKGWKVERINGNGEGKCGSXALDAILPPPRPTDQALRLPLPDV 202          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_METTP (Elongation factor 1-alpha OS=Methanosaeta thermophila (strain DSM 6194 / PT) GN=tuf PE=3 SV=1)

HSP 1 Score: 275.018 bits (702), Expect = 3.328e-73
Identity = 127/241 (52.70%), Postives = 172/241 (71.37%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            M   K H+N+  IGHVD GKST  G ++Y++G ID+ +IE++ KEAA   K +F++AWV+D LK ERERG+TIDIA  +F+T KYY TV+D PGHRDF+KNMITG SQAD AVL++ +  G          QT+EH  LA TLGV Q+I   NKMDAT P Y + RY+E+ +EV   L+ VGY  ++VPF+P+S + GDN+++ S    WY GPT+LDAL+ + EP++P + PLR+P+QDV
Sbjct:    1 MADTKPHLNLAFIGHVDHGKSTLVGRMMYEMGAIDEHIIEQYRKEAAAKGKATFEFAWVMDSLKEERERGVTIDIAHQRFDTDKYYFTVVDCPGHRDFVKNMITGASQADAAVLVVAAPDGVM-------AQTKEHVFLARTLGVNQLIVAINKMDATEPPYDEKRYNEVKEEVGKLLRMVGYKIDEVPFIPVSAYNGDNVVKHSDRTKWYTGPTVLDALNALKEPQKPVNLPLRIPVQDV 234          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_THEAC (Elongation factor 1-alpha OS=Thermoplasma acidophilum GN=tuf PE=3 SV=2)

HSP 1 Score: 274.248 bits (700), Expect = 5.677e-73
Identity = 131/241 (54.36%), Postives = 176/241 (73.03%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            M  +K H+N++ IGHVD GKST  G L+Y+ G I   +IE + KEA +  K +F++AWV+D+ K ERERG+TID+A  KFET KYY T+IDAPGHRDF+KNMITGTSQAD A+L+I +  G    G+ +  QTREHA LA TLGV QM+   NKMDAT+P YS+ RY+E+  +    L+ +G+  + + FVPISG++GDN+ + S N+ WYKGPTLL ALD    P++P +KPLR+P++DV
Sbjct:    1 MASQKPHLNLITIGHVDHGKSTLVGRLLYEHGEIPAHIIEEYRKEAEQKGKATFEFAWVMDRFKEERERGVTIDLAHRKFETDKYYFTLIDAPGHRDFVKNMITGTSQADAAILVISAREG---EGVME--QTREHAFLARTLGVPQMVVAINKMDATSPPYSEKRYNEVKADAEKLLRSIGF--KDISFVPISGYKGDNVTKPSPNMPWYKGPTLLQALDAFKVPEKPINKPLRIPVEDV 234          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_SULSO (Elongation factor 1-alpha OS=Sulfolobus solfataricus GN=tuf PE=1 SV=3)

HSP 1 Score: 273.092 bits (697), Expect = 1.265e-72
Identity = 124/238 (52.10%), Postives = 173/238 (72.69%), Query Frame = 3
Query:   87 EKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            +K H+N++VIGH+D GKST  G L+   G ID++ ++  E+ A ++ K S K+A++LD+LK ERERG+TI++   +FET KY+ T+IDAPGHRDF+KNMITG SQAD A+L++ +  G +EAG+S +GQTREH +LA T+G+ Q+I   NKMD T P Y + RY EIV +VS +++  G+N  KV FVP+    GDN+  RS N+ WY GPTL + LD +  P +P DKPLR+P+QDV
Sbjct:    3 QKPHLNLIVIGHIDHGKSTLVGRLLMDRGFIDEKTVKEAEEAAKKLGKESEKFAFLLDRLKEERERGVTINLTFMRFETKKYFFTIIDAPGHRDFVKNMITGASQADAAILVVSAKKGEYEAGMSVEGQTREHIILAKTMGLDQLIVAVNKMDLTEPPYDEKRYKEIVDQVSKFMRSYGFNTNKVRFVPVVAPAGDNITHRSENMKWYNGPTLEEYLDQLELPPKPVDKPLRIPIQDV 240          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_PICTO (Elongation factor 1-alpha OS=Picrophilus torridus GN=tuf PE=3 SV=1)

HSP 1 Score: 273.092 bits (697), Expect = 1.265e-72
Identity = 129/241 (53.53%), Postives = 179/241 (74.27%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            M  EK H+N+V+IGHVD GKST  G L+++ G I + +I+ ++KEA E  K +F++AWV+D+ K ERERG+TID+   KFET KYY T+IDAPGHRDF+KNMITGTSQAD AVL++ +  G    G+    QT+EHA LA TLGV Q+I   NKMDAT P YS+ R++E+  E++  L  +G+  + VP +P+SG++GDN+++ S NL W+KGPTL++AL+ +  P +P DKPLRLP++DV
Sbjct:    1 MASEKPHMNLVIIGHVDHGKSTLVGRLLFEHGEIPQHIIDEYKKEAEEKGKATFEFAWVMDRFKEERERGVTIDLTHRKFETDKYYFTIIDAPGHRDFVKNMITGTSQADAAVLVVSAREG---EGVM--AQTKEHAFLARTLGVPQLIAVVNKMDATQPPYSEKRFNEVKDEITKLLTPIGF--KNVPIIPLSGYKGDNIMKPSPNLSWWKGPTLMEALNALQVPAKPVDKPLRLPVEDV 234          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_METMJ (Elongation factor 1-alpha OS=Methanoculleus marisnigri (strain ATCC 35101 / DSM 1498 / JR1) GN=tuf PE=3 SV=1)

HSP 1 Score: 270.781 bits (691), Expect = 6.277e-72
Identity = 128/241 (53.11%), Postives = 174/241 (72.20%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            M  EK H+N+ VIGH+D GKSTT G L+++ G +   +IE F KEA    K SF++AWV+D LK ERERGITIDIA  +F+T KYY TV+D PGHRDF+KNMITG SQAD A+L++ +  G  E       QT+EH  L+ TLG+ Q+I   NKMDA   KY + RY+E+ +++S  LK VGY P+ + F+P+S F GDN+ ++S N  WYKGPT+LDAL+ ++EP++P++ P+RLP+QDV
Sbjct:    1 MAAEKPHMNLAVIGHIDHGKSTTVGRLLFETGTVPPHIIESFRKEAESKGKGSFEFAWVMDSLKEERERGITIDIAHKRFDTDKYYFTVVDCPGHRDFVKNMITGASQADAALLVVAAPDGVME-------QTKEHVFLSRTLGINQLIIGINKMDAA--KYDEKRYNEVKEQLSQLLKMVGYKPDDISFIPMSAFVGDNIAKKSENTPWYKGPTVLDALNALSEPEKPTNLPMRLPIQDV 232          
The following BLAST results are available for this feature:
BLAST of CN186363 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
EF1A_IGNH43.922e-8257.68Elongation factor 1-alpha OS=Ignicoccus hospitalis... [more]
EF1A_GIALA3.922e-8266.22Elongation factor 1-alpha (Fragment) OS=Giardia la... [more]
EF1A_AERPE4.337e-8156.30Elongation factor 1-alpha OS=Aeropyrum pernix GN=t... [more]
EF1A_THEPD4.200e-7655.37Elongation factor 1-alpha OS=Thermofilum pendens (... [more]
EF1A_RHYAM2.305e-7470.65Elongation factor 1-alpha (Fragment) OS=Rhynchosci... [more]
EF1A_METTP3.328e-7352.70Elongation factor 1-alpha OS=Methanosaeta thermoph... [more]
EF1A_THEAC5.677e-7354.36Elongation factor 1-alpha OS=Thermoplasma acidophi... [more]
EF1A_SULSO1.265e-7252.10Elongation factor 1-alpha OS=Sulfolobus solfataric... [more]
EF1A_PICTO1.265e-7253.53Elongation factor 1-alpha OS=Picrophilus torridus ... [more]
EF1A_METMJ6.277e-7253.11Elongation factor 1-alpha OS=Methanoculleus marisn... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0002M13_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0002M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN186363 ID=CN186363; Name=CN186363; organism=Citrus sinensis; type=EST; length=802bp
GCGGTTTTCCGAAGGGCGGCGAGAGAGAACCATTTTCGCGAATATCATTT
TAATAAGGTTTTTTCTAGATTTGCCTAATGGGTAAGGAGAAGACTCACAT
CAACATTGTGGTCATCGGCCATGTCGACTCTGGAAAGTCGACCACCACCG
GACACTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATCGAGAGG
TTCGAGAAGGAAGCTGCTGAGATGAACAAGAGGTCATTCAAATACGCCTG
GGTGCTCGACAAGCTCAAGGCTGAGCGTGAACGTGGTATTACCATTGATA
TTGCCTTGTGGAAGTTCGAGACCACCAAGTACTACTGCACCGTCATTGAT
GCTCCCGGACATCGTGACTTTATCAAGAACATGATTACCGGTACCTCACA
GGCTGACTGTGCTGTCCTCATCATTGACTCTACCACTGGTGGTTTTGAAG
CTGGTATCTCCAAGGATGGTCAGACTCGTGAGCATGCTTTGCTTGCTTTC
ACCCTTGGTGTGAAGCAGATGATTTGCTGCTGTAACAAGATGGACGCCAC
CACCCCCAAGTATTCAAAGGCTAGGTACGATGAAATTGTGAAGGAGGTCT
CTTCCTACCTGAAGAAGGTTGGATACAACCCCGAGAAAGTCCCATTCGTG
CCCATCTCTGGTTTCGAGGGTGACAACATGATTGAGAGGTCTACCAACCT
TGACTGGTACAAGGGTCCAACCCTGCTGGACGCTCTTGACAATATCAATG
AGCCGAAGAGGCCCTCAGACAAGCCCCTTCGTCTGCCACTCCAGGACGTG
TA
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