CN186363

Overview
NameCN186363
Unique NameCN186363
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length802
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: ERF3A_HUMAN (Eukaryotic peptide chain release factor GTP-binding subunit ERF3A OS=Homo sapiens GN=GSPT1 PE=1 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 1.228e-59
Identity = 112/238 (47.06%), Postives = 158/238 (66.39%), Query Frame = 3
Query:   87 EKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEK-VPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQD 797
            +K H+N+V IGHVD+GKST  G ++Y  G +DKR +E++E+EA E N+ ++  +W LD  + ER++G T+++    FET K + T++DAPGH+ F+ NMI G SQAD AVL+I +  G FE G  K GQTREHA+LA T GVK +I   NKMD  T  +S  RY+E  +++  +LKKVG+NP+K + F+P SG  G N+ E+S    WY G   +  LDN+    R  D P+RLP+ D
Sbjct:   71 KKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVD 308          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_METTH (Elongation factor 1-alpha OS=Methanobacterium thermoautotrophicum GN=tuf PE=3 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 2.735e-59
Identity = 114/241 (47.30%), Postives = 156/241 (64.73%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            M KEK H+N+  IGHVD GKST  GHL+ + G I ++ +   E           K+ +V+D+L  ERERG+TID+A  KFET KY  T++D PGHRDF+KNMITG SQAD AVL++    G          QT+EH  L+ TLG+ Q+I   NKMD     Y + +++ +  EV++ +K VGY P  V F+P+S FEGDN+  +S N  WYKG TL++ALD++  P++P D PLR+P+QDV
Sbjct:    1 MAKEKEHMNLAFIGHVDHGKSTLVGHLLLQAGAIAEQQLAEGED----------KFRFVMDRLSEERERGVTIDLAHAKFETDKYEFTIVDCPGHRDFVKNMITGASQADAAVLVVAVDDGVMP-------QTKEHVFLSRTLGINQLIVAINKMDLVN--YDEEKFNALKDEVAALIKTVGYKPSDVEFIPLSAFEGDNITSKSENTPWYKGKTLVEALDDLEAPEKPVDLPLRIPIQDV 222          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_HALMA (Elongation factor 1-alpha OS=Haloarcula marismortui GN=tuf PE=1 SV=2)

HSP 1 Score: 228.024 bits (580), Expect = 4.666e-59
Identity = 112/238 (47.06%), Postives = 157/238 (65.97%), Query Frame = 3
Query:   87 EKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            ++ H N+ +IGHVD GKST  G L+Y+ G + + VIE+ ++EA E  K  F++A+V+D L  ERERG+TIDIA  +F T  Y  T++D PGHRDF+KNMITG SQAD AVL++ +  G          QT+EH  LA TLG+ ++I   NKMD     Y ++ Y ++V+EV   L +V ++ E   F+P+S FEGDN+ E S +  WY G  LL+AL+ +  P+ P+D PLRLP+QDV
Sbjct:    3 DEQHQNLAIIGHVDHGKSTLVGRLLYETGSVPEHVIEQHKEEAEEKGKGGFEFAYVMDNLAEERERGVTIDIAHQEFSTDTYDFTIVDCPGHRDFVKNMITGASQADNAVLVVAADDG-------VQPQTQEHVFLARTLGIGELIVAVNKMDLV--DYGESEYKQVVEEVKDLLTQVRFDSENAKFIPVSAFEGDNIAEESEHTGWYDGEILLEALNELPAPEPPTDAPLRLPIQDV 231          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_NANEQ (Elongation factor 1-alpha OS=Nanoarchaeum equitans GN=tuf PE=3 SV=1)

HSP 1 Score: 224.942 bits (572), Expect = 3.950e-58
Identity = 113/240 (47.08%), Postives = 157/240 (65.42%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEK-VPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQ 794
            M +EK HIN+V IGHVD GKSTT G L Y LG I +  +E+  +EA +  K  F +A+++D+ K ER RG+TIDIA  + ET   Y T++DAPGH+DF+KNMITG SQAD AVL++ +  G  E       QT+EHA+LA T G+ Q+I   NKMD     Y + R++E+  +V   LK +GY  E  +  +P + F GDN++++S  + WY GPTL +ALD +  P+ P D PLR+P+Q
Sbjct:    1 MPREKPHINVVFIGHVDHGKSTTVGRLKYDLGLIPESELEKIREEAKKYGKEEFVFAYLMDRQKEERARGVTIDIAHTELETPHNYITIVDAPGHKDFVKNMITGASQADAAVLVVAADDGVQE-------QTQEHAVLARTFGINQIIVYINKMDKV--NYDQKRFEEVKNQVLKLLKMIGYKDENIIAVIPGASFHGDNVVKKSDKMPWYNGPTLYEALDMLKPPQLPVDLPLRIPIQ 231          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: ERF3B_PONAB (Eukaryotic peptide chain release factor GTP-binding subunit ERF3B OS=Pongo abelii GN=GSPT2 PE=2 SV=1)

HSP 1 Score: 224.557 bits (571), Expect = 5.158e-58
Identity = 109/238 (45.80%), Postives = 158/238 (66.39%), Query Frame = 3
Query:   87 EKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEK-VPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQD 797
            +K H+N+V IGHVD+GKST  G +++  G +DKR +E++E+EA E N+ ++  +W LD  + ER++G T+++    FET + + T++DAPGH+ F+ NMI G SQAD AVL+I +  G FE G  K GQTREHA+LA T GVK +I   NKMD  T  +S  RY+E  +++  +LKKVG++P+K + F+P SG  G N+ E+S    WY G   +  LDN+    R  D P+RLP+ D
Sbjct:  200 KKEHVNVVFIGHVDAGKSTIGGQIMFLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETERKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSIERYEECKEKLVPFLKKVGFSPKKDIHFMPCSGLTGANVKEQSDFCPWYTGLPFIPYLDNLPNFNRSIDGPIRLPIVD 437          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: ERF3B_HUMAN (Eukaryotic peptide chain release factor GTP-binding subunit ERF3B OS=Homo sapiens GN=GSPT2 PE=1 SV=2)

HSP 1 Score: 224.557 bits (571), Expect = 5.158e-58
Identity = 109/238 (45.80%), Postives = 158/238 (66.39%), Query Frame = 3
Query:   87 EKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEK-VPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQD 797
            +K H+N+V IGHVD+GKST  G +++  G +DKR +E++E+EA E N+ ++  +W LD  + ER++G T+++    FET + + T++DAPGH+ F+ NMI G SQAD AVL+I +  G FE G  K GQTREHA+LA T GVK +I   NKMD  T  +S  RY+E  +++  +LKKVG++P+K + F+P SG  G N+ E+S    WY G   +  LDN+    R  D P+RLP+ D
Sbjct:  200 KKEHVNVVFIGHVDAGKSTIGGQIMFLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETERKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSIERYEECKEKLVPFLKKVGFSPKKDIHFMPCSGLTGANIKEQSDFCPWYTGLPFIPYLDNLPNFNRSIDGPIRLPIVD 437          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: ERF3B_MOUSE (Eukaryotic peptide chain release factor GTP-binding subunit ERF3B OS=Mus musculus GN=Gspt2 PE=1 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 1.501e-57
Identity = 108/238 (45.38%), Postives = 158/238 (66.39%), Query Frame = 3
Query:   87 EKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEK-VPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQD 797
            +K H+N+V IGHVD+GKST  G +++  G +D+R +E++E+EA E N+ ++  +W LD  + ER++G T+++    FET K + T++DAPGH+ F+ NMI G SQAD AVL+I +  G FE G  K GQTREHA+LA T GVK +I   NKMD  T  +S  RY+E  +++  +LKKVG++P+K + F+P SG  G N+ E+S    WY G   +  LD++    R  D P+RLP+ D
Sbjct:  204 KKEHVNVVFIGHVDAGKSTIGGQIMFLTGMVDRRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKYLIVLINKMDDPTVDWSSERYEECKEKLVPFLKKVGFSPKKDIHFMPCSGLTGANIKEQSDFCPWYTGLPFIPYLDSLPNFNRSIDGPIRLPIVD 441          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: HBS1_SCHPO (Elongation factor 1 alpha-like protein OS=Schizosaccharomyces pombe GN=hbs1 PE=2 SV=4)

HSP 1 Score: 219.55 bits (558), Expect = 1.659e-56
Identity = 113/240 (47.08%), Postives = 156/240 (65.00%), Query Frame = 3
Query:   90 KTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYL-KKVGYNPEKVPFVPISGFEGDNMIERSTN--LDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            K  +++VV GHVDSGKST  G ++++LG I+ R +++   EAA   K SF YAW+LD  + ER RG+T+D+A   FE+ K    + DAPGHRDFI  MI G S AD AVL++DS+   FE G  ++GQTREHA L   LG+ +++   NK+D  +  +S+ R+ EI   VS +L K VG+    V FVPIS   G N+I++ ++    WYKGPTLL ALD +  P++P  KPLRL + DV
Sbjct:  175 KPVVHLVVTGHVDSGKSTMLGRIMFELGEINSRSMQKLHNEAANSGKGSFSYAWLLDTTEEERARGVTMDVASTTFESDKKIYEIGDAPGHRDFISGMIAGASSADFAVLVVDSSQNNFERGFLENGQTREHAYLLRALGISEIVVSVNKLDLMS--WSEDRFQEIKNIVSDFLIKMVGFKTSNVHFVPISAISGTNLIQKDSSDLYKWYKGPTLLSALDQLVPPEKPYRKPLRLSIDDV 412          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: ERF3_ZYGRO (Eukaryotic peptide chain release factor GTP-binding subunit OS=Zygosaccharomyces rouxii GN=SUP35 PE=3 SV=2)

HSP 1 Score: 218.009 bits (554), Expect = 4.828e-56
Identity = 105/237 (44.30%), Postives = 155/237 (65.40%), Query Frame = 3
Query:   90 KTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYN-PEKVPFVPISGFEGDNMIER--STNLDWYKGPTLLDALDNINEPKRPSDKPLRLPL 791
            K H++I+ +GHVD+GKST  G+++Y  G +DKR +E++E+EA +  K+ +  +WV+D  + ER+ G TI++    FET K   T++DAPGH+ ++  MI G SQAD  +L+I +  G +E G  K GQTREHALLA T GV ++I   NKMD  T  +SK RYD+ VK +S++LK +GYN  E+V F+P+SG+ G  +  R       WY GP LL+ +DN++   R  + P  LP+
Sbjct:  235 KDHMSIIFMGHVDAGKSTMGGNILYMTGSVDKRTVEKYEREAKDAGKQGWYLSWVMDTNREERDDGKTIEVGRAYFETEKRRYTILDAPGHKMYVSEMIGGASQADVGILVISARKGEYETGFEKGGQTREHALLAKTQGVNKLIVTINKMDDPTVNWSKERYDQCVKNLSNFLKAIGYNVKEEVVFMPVSGYSGAGLGTRVDPKECPWYDGPALLEYMDNMSHVDRKMNAPFMLPI 471          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: ERF3_YEAST (Eukaryotic peptide chain release factor GTP-binding subunit OS=Saccharomyces cerevisiae GN=SUP35 PE=1 SV=1)

HSP 1 Score: 216.468 bits (550), Expect = 1.405e-55
Identity = 106/237 (44.73%), Postives = 152/237 (64.14%), Query Frame = 3
Query:   90 KTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYN-PEKVPFVPISGFEGDNMIER--STNLDWYKGPTLLDALDNINEPKRPSDKPLRLPL 791
            K H++++ +GHVD+GKST  G+L+Y  G +DKR IE++E+EA +  ++ +  +WV+D  K ER  G TI++    FET K   T++DAPGH+ ++  MI G SQAD  VL+I +  G +E G  + GQTREHALLA T GV +M+   NKMD  T  +SK RYD+ V  VS++L+ +GYN    V F+P+SG+ G N+ +        WY GPTLL+ LD +N   R  + P  LP+
Sbjct:  258 KDHVSLIFMGHVDAGKSTMGGNLLYLTGSVDKRTIEKYEREAKDAGRQGWYLSWVMDTNKEERNDGKTIEVGKAYFETEKRRYTILDAPGHKMYVSEMIGGASQADVGVLVISARKGEYETGFERGGQTREHALLAKTQGVNKMVVVVNKMDDPTVNWSKERYDQCVSNVSNFLRAIGYNIKTDVVFMPVSGYSGANLKDHVDPKECPWYTGPTLLEYLDTMNHVDRHINAPFMLPI 494          
The following BLAST results are available for this feature:
BLAST of CN186363 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
ERF3A_HUMAN1.228e-5947.06Eukaryotic peptide chain release factor GTP-bindin... [more]
EF1A_METTH2.735e-5947.30Elongation factor 1-alpha OS=Methanobacterium ther... [more]
EF1A_HALMA4.666e-5947.06Elongation factor 1-alpha OS=Haloarcula marismortu... [more]
EF1A_NANEQ3.950e-5847.08Elongation factor 1-alpha OS=Nanoarchaeum equitans... [more]
ERF3B_PONAB5.158e-5845.80Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3B_HUMAN5.158e-5845.80Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3B_MOUSE1.501e-5745.38Eukaryotic peptide chain release factor GTP-bindin... [more]
HBS1_SCHPO1.659e-5647.08Elongation factor 1 alpha-like protein OS=Schizosa... [more]
ERF3_ZYGRO4.828e-5644.30Eukaryotic peptide chain release factor GTP-bindin... [more]
ERF3_YEAST1.405e-5544.73Eukaryotic peptide chain release factor GTP-bindin... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0002M13_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0002M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN186363 ID=CN186363; Name=CN186363; organism=Citrus sinensis; type=EST; length=802bp
GCGGTTTTCCGAAGGGCGGCGAGAGAGAACCATTTTCGCGAATATCATTT
TAATAAGGTTTTTTCTAGATTTGCCTAATGGGTAAGGAGAAGACTCACAT
CAACATTGTGGTCATCGGCCATGTCGACTCTGGAAAGTCGACCACCACCG
GACACTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATCGAGAGG
TTCGAGAAGGAAGCTGCTGAGATGAACAAGAGGTCATTCAAATACGCCTG
GGTGCTCGACAAGCTCAAGGCTGAGCGTGAACGTGGTATTACCATTGATA
TTGCCTTGTGGAAGTTCGAGACCACCAAGTACTACTGCACCGTCATTGAT
GCTCCCGGACATCGTGACTTTATCAAGAACATGATTACCGGTACCTCACA
GGCTGACTGTGCTGTCCTCATCATTGACTCTACCACTGGTGGTTTTGAAG
CTGGTATCTCCAAGGATGGTCAGACTCGTGAGCATGCTTTGCTTGCTTTC
ACCCTTGGTGTGAAGCAGATGATTTGCTGCTGTAACAAGATGGACGCCAC
CACCCCCAAGTATTCAAAGGCTAGGTACGATGAAATTGTGAAGGAGGTCT
CTTCCTACCTGAAGAAGGTTGGATACAACCCCGAGAAAGTCCCATTCGTG
CCCATCTCTGGTTTCGAGGGTGACAACATGATTGAGAGGTCTACCAACCT
TGACTGGTACAAGGGTCCAACCCTGCTGGACGCTCTTGACAATATCAATG
AGCCGAAGAGGCCCTCAGACAAGCCCCTTCGTCTGCCACTCCAGGACGTG
TA
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