CN186363

Overview
NameCN186363
Unique NameCN186363
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length802
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: CYSN_SALNS (Sulfate adenylyltransferase subunit 1 OS=Salmonella newport (strain SL254) GN=cysN PE=3 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 3.723e-32
Identity = 78/241 (32.37%), Postives = 132/241 (54.77%), Query Frame = 3
Query:   84 KEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKY--AWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            + K+ +  +  G VD GKST  G L++    I +  +     ++     +  K   A ++D L+AERE+GITID+A   F T K    + D PGH  + +NM TG S  D A+L+ID+  G  +       QTR H+ ++  LG+K ++   NKMD     Y +  +  I ++  ++ +++  + + + FVP+S  EGDN+  +S N+ W+ GPTLL+ L+ ++  +    +P+R P+Q V
Sbjct:   23 QHKSLLRFLTCGSVDDGKSTLIGRLLHDTLQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCDLAILLIDARKGVLD-------QTRRHSFISTLLGIKHLVVAINKMDLV--DYREETFARIREDYLTFAEQLPGDLD-IRFVPLSALEGDNVAAQSANMRWHSGPTLLEVLETVDIQRAVDRQPMRFPVQYV 253          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: CYSN_AGRT5 (Sulfate adenylyltransferase subunit 1 OS=Agrobacterium tumefaciens (strain C58 / ATCC 33970) GN=cysN PE=3 SV=1)

HSP 1 Score: 138.272 bits (347), Expect = 4.862e-32
Identity = 79/243 (32.51%), Postives = 132/243 (54.32%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFK-YAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMI-ERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            + ++   + ++  G VD GKST  G L++    + +       +++ + N      +A +LD L+AERE+GITID+A   F T +    V D PGH  + +NM TG S AD AVL++D+ TG  E       QTR HA +A  +G++Q +   NK+D T   Y KA ++ I  E   +   +G   +++  +P+S  +G+N++     ++ WY+GPTL++ L+ +   +       RLP+Q V
Sbjct:   24 VARDTRPLRLITCGSVDDGKSTLIGRLLWDTKAVKEDQAATLHRDSGKQNDLGLPDFALLLDGLQAEREQGITIDVAYRYFATDRRAFIVADTPGHEQYTRNMATGASTADLAVLLVDARTGILE-------QTRRHATIAALMGIRQFVLAVNKIDLT--NYDKAGFELIAHEFRDFASDLGI--KQITAIPMSALKGENVVLSGKASMPWYEGPTLVETLE-LATVRSTQSGGFRLPVQRV 254          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: CYSN_TOLAT (Sulfate adenylyltransferase subunit 1 OS=Tolumonas auensis (strain DSM 9187 / TA4) GN=cysN PE=3 SV=1)

HSP 1 Score: 137.887 bits (346), Expect = 6.350e-32
Identity = 80/239 (33.47%), Postives = 124/239 (51.88%), Query Frame = 3
Query:   84 KEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFK--YAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQ 794
            + K+ +  +  G VD GKST  G L++    + +  ++    ++ ++     K  +A ++D L+AERE+GITID+A   F T K    + D PGH  + +NM TG S  D A+++ID+  G  E       QTR H+ +A  LG++Q I   NKMD     + +  +D I KE   +    G     +  VPIS  +G+N++  S    WY+G  LL  L+N     R  D P R+P+Q
Sbjct:   22 QHKSLLRFLTCGSVDDGKSTLIGRLLHDSRQVYEDQLKTLHSDSQKIGTTGEKLDFALLVDGLQAEREQGITIDVAYRYFSTPKRKFIIADTPGHEQYTRNMATGASTCDLAIILIDARRGVLE-------QTRRHSFIASLLGIRQFIVAVNKMDLV--NFDEKVFDSIKKEYLEFAS--GLPKTDIRVVPISALDGENLVVNSELTPWYQGEPLLTMLENAEVGTRDLDLPFRMPVQ 249          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: NODQ_RHITR (Bifunctional enzyme nodQ OS=Rhizobium tropici GN=nodQ PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 8.293e-32
Identity = 84/240 (35.00%), Postives = 129/240 (53.75%), Query Frame = 3
Query:   99 INIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEM--------NKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQ 794
            +  +  G VD GKST  G L+      D +++  FE + A++        N     ++ VLD L+AERE+GITID+A   F T+K    V D PGH ++ +NM TG S AD AV+++DS  G  +       QTR H+ LA  LG++ ++   NK+D     +S+  +D IV E   +  K+G+    V  +P+S   GDN++ +S  L WY+G  LL+ L+ +        +P R P+Q
Sbjct:   25 LRFITCGSVDDGKSTLIGRLL-----CDAQLV--FEDQLADLRHGGIRGGNGEEIDFSLVLDGLEAEREQGITIDVAYRYFSTSKRKFIVADTPGHFEYTRNMATGASTADLAVILVDSRQGILQ-------QTRRHSYLASLLGIRHVVLAVNKIDLI--DFSEPIFDAIVAEYVRFSAKLGF--ASVMAIPMSARFGDNVVSKSGKLPWYQGSPLLEYLETVELDAPDRQEPFRFPVQ 246          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: CYSN_SULNB (Sulfate adenylyltransferase subunit 1 OS=Sulfurovum sp. (strain NBC37-1) GN=cysN PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 8.293e-32
Identity = 81/246 (32.93%), Postives = 130/246 (52.85%), Query Frame = 3
Query:   84 KEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSF-------KYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            + K  +  +  G VD GKST  G ++Y     D ++I   +  AAE   R +         A ++D L++ERE+GITID+A   F T K    + D PGH  + +NM+TG S AD A+++ID+  G          QTR H+ +   LG++ +I   NKMD     +S+  +D+I     +   ++G   +   ++P+S  EGDN+++RS    W+ G  LL  LD+++  K P ++  R P+Q V
Sbjct:   20 ENKDMLRFLTCGSVDDGKSTLIGRMLY-----DSKMIFDDQLSAAEGESRKYGTTGEKLDMALLVDGLQSEREQGITIDVAYRFFATEKRKFIIADTPGHEQYTRNMVTGASTADVAIILIDARKGILT-------QTRRHSFIVNLLGIEHVIVAINKMDLV--DFSEEVFDKISGAYGALADELGI--KNTYYIPVSALEGDNVVDRSKRSPWFSGQPLLGLLDSMDISKEPKEEDFRFPVQYV 249          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: CYSN_SACD2 (Sulfate adenylyltransferase subunit 1 OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=cysN PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 8.293e-32
Identity = 80/241 (33.20%), Postives = 128/241 (53.11%), Query Frame = 3
Query:   84 KEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKY--AWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            ++K  +  +  G VD GKST  G L++    I +  +E    + ++      K   A ++D L+AERE+GITID+A   F T K    + D PGH  + +NM TG S  D A+++ID+  G          QTR H+ +A  LG+K ++   NKMD     YS+  ++ I +      K +G   E + FVP+S  +GDN++ +S N+ WY G +L++ L+++      + K  R P+Q V
Sbjct:   20 EQKELLRFLTCGSVDDGKSTLIGRLLHDSKMIYEDQLEAVRNDNSKHGTTGDKVDLALLVDGLQAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRNMATGASTCDMAIILIDARHGVMT-------QTRRHSFIASLLGIKHLVIAINKMDLV--DYSQETFESIKQAYGEVAKTLG--QENLYFVPMSALDGDNVVNKSENMPWYTGESLMEILESVQITGAKNLKDFRYPVQYV 249          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: NODQ_RHIS3 (Bifunctional enzyme nodQ OS=Rhizobium sp. (strain N33) GN=nodQ PE=3 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.415e-31
Identity = 76/241 (31.54%), Postives = 133/241 (55.19%), Query Frame = 3
Query:   84 KEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRS--FKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            ++K+ +  +  G VD GKST  G L+     I +  +   E+++ +         +A ++D L+AERE+GITID+A   F T K    V D PGH  + +NM TG S AD A+++ID+  G          QTR H+++A  LG++ ++   NK+D     + +A ++ I +    + + +G+  + +  +P+S   GDN+  RS +++WY GPTL++ L+ ++  +   + P R P+Q V
Sbjct:   23 EKKSLLRFLTCGSVDDGKSTLIGRLLSDTKQIFEDQLAALERDSRKHGTTGDDIDFALLVDGLEAEREQGITIDVAYRFFATPKRKFIVADTPGHEQYTRNMATGASTADLAIVLIDARQGVLR-------QTRRHSIIASLLGIRHIVLAVNKIDLV--GFDQAVFERITESYRQFSRDLGF--QTIVPIPMSARYGDNVTSRSESMEWYSGPTLIEHLETVSVEEAVVELPFRFPVQYV 252          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: CYSN_SHIF8 (Sulfate adenylyltransferase subunit 1 OS=Shigella flexneri serotype 5b (strain 8401) GN=cysN PE=3 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.415e-31
Identity = 78/241 (32.37%), Postives = 131/241 (54.36%), Query Frame = 3
Query:   84 KEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKY--AWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            + K+ +  +  G VD GKST  G L++    I +  +     ++     +  K   A ++D L+AERE+GITID+A   F T K    + D PGH  + +NM TG S  + A+L+ID+  G  +       QTR H+ ++  LG+K ++   NKMD     YS+  +  I ++  ++  ++  N + + FVP+S  EGDN+  +S ++ WY G TLL+ L+ +   +  + +P+R P+Q V
Sbjct:   23 QHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLD-------QTRRHSFISTLLGIKHLVVAINKMDLV--DYSEETFTRIREDYLTFAGQLPGNLD-IRFVPLSALEGDNVASQSESMPWYSGLTLLEVLETVEIQRVVNAQPMRFPVQYV 253          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: CYSN_SHIFL (Sulfate adenylyltransferase subunit 1 OS=Shigella flexneri GN=cysN PE=3 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 1.847e-31
Identity = 78/241 (32.37%), Postives = 130/241 (53.94%), Query Frame = 3
Query:   84 KEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKY--AWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            + K+ +  +  G VD GKST  G L++    I +  +     ++     +  K   A ++D L+AERE+GITID+A   F T K    + D PGH  + +NM TG S  + A+L+ID+  G  +       QTR H+ ++  LG+K ++   NKMD     YS+  +  I ++  ++  ++  N + + FVP+S  EGDN+  +S ++ WY G TLL+ L+ +   +    +P+R P+Q V
Sbjct:   23 QHKSLLRFLTCGSVDDGKSTLIGRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYFSTEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLD-------QTRRHSFISTLLGIKHLVVAINKMDLV--DYSEETFTRIREDYLTFAGQLPGNLD-IRFVPLSALEGDNVASQSESMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYV 253          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: CYSN_RHIME (Sulfate adenylyltransferase subunit 1 OS=Rhizobium meliloti GN=cysN PE=3 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 1.847e-31
Identity = 81/237 (34.18%), Postives = 130/237 (54.85%), Query Frame = 3
Query:   99 INIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAA-EMNKRSFK-YAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMI-ERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            + ++  G VD GKST  G L++    + +      +++++ + N      +A +LD L+AERE+GITID+A   F T K    V D PGH  + +NM TG S AD AVL++D+  G  E       QTR HA +A  +G++Q +   NK+D T   Y +AR+D+I  E       +G    +V  +P+S  +G+N++ +   ++ WY GPTL++ L+ +   +       RLP+Q V
Sbjct:   33 LRLITCGSVDDGKSTLIGRLLWDTKAVKEDQAASLQRDSSGKQNDLGLPDFALLLDGLQAEREQGITIDVAYRYFATDKRSFIVADTPGHEQYTRNMATGASTADLAVLLVDARVGLLE-------QTRRHATIATLMGIRQFVLAVNKIDLT--NYDRARFDQISHEFRELALSLGV--RQVTAIPVSALKGENVVYDGRASMPWYDGPTLIEILE-LATTRSAQTVGFRLPVQRV 257          
The following BLAST results are available for this feature:
BLAST of CN186363 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
CYSN_SALNS3.723e-3232.37Sulfate adenylyltransferase subunit 1 OS=Salmonell... [more]
CYSN_AGRT54.862e-3232.51Sulfate adenylyltransferase subunit 1 OS=Agrobacte... [more]
CYSN_TOLAT6.350e-3233.47Sulfate adenylyltransferase subunit 1 OS=Tolumonas... [more]
NODQ_RHITR8.293e-3235.00Bifunctional enzyme nodQ OS=Rhizobium tropici GN=n... [more]
CYSN_SULNB8.293e-3232.93Sulfate adenylyltransferase subunit 1 OS=Sulfurovu... [more]
CYSN_SACD28.293e-3233.20Sulfate adenylyltransferase subunit 1 OS=Saccharop... [more]
NODQ_RHIS31.415e-3131.54Bifunctional enzyme nodQ OS=Rhizobium sp. (strain ... [more]
CYSN_SHIF81.415e-3132.37Sulfate adenylyltransferase subunit 1 OS=Shigella ... [more]
CYSN_SHIFL1.847e-3132.37Sulfate adenylyltransferase subunit 1 OS=Shigella ... [more]
CYSN_RHIME1.847e-3134.18Sulfate adenylyltransferase subunit 1 OS=Rhizobium... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0002M13_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0002M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN186363 ID=CN186363; Name=CN186363; organism=Citrus sinensis; type=EST; length=802bp
GCGGTTTTCCGAAGGGCGGCGAGAGAGAACCATTTTCGCGAATATCATTT
TAATAAGGTTTTTTCTAGATTTGCCTAATGGGTAAGGAGAAGACTCACAT
CAACATTGTGGTCATCGGCCATGTCGACTCTGGAAAGTCGACCACCACCG
GACACTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATCGAGAGG
TTCGAGAAGGAAGCTGCTGAGATGAACAAGAGGTCATTCAAATACGCCTG
GGTGCTCGACAAGCTCAAGGCTGAGCGTGAACGTGGTATTACCATTGATA
TTGCCTTGTGGAAGTTCGAGACCACCAAGTACTACTGCACCGTCATTGAT
GCTCCCGGACATCGTGACTTTATCAAGAACATGATTACCGGTACCTCACA
GGCTGACTGTGCTGTCCTCATCATTGACTCTACCACTGGTGGTTTTGAAG
CTGGTATCTCCAAGGATGGTCAGACTCGTGAGCATGCTTTGCTTGCTTTC
ACCCTTGGTGTGAAGCAGATGATTTGCTGCTGTAACAAGATGGACGCCAC
CACCCCCAAGTATTCAAAGGCTAGGTACGATGAAATTGTGAAGGAGGTCT
CTTCCTACCTGAAGAAGGTTGGATACAACCCCGAGAAAGTCCCATTCGTG
CCCATCTCTGGTTTCGAGGGTGACAACATGATTGAGAGGTCTACCAACCT
TGACTGGTACAAGGGTCCAACCCTGCTGGACGCTCTTGACAATATCAATG
AGCCGAAGAGGCCCTCAGACAAGCCCCTTCGTCTGCCACTCCAGGACGTG
TA
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