CN186363

Overview
NameCN186363
Unique NameCN186363
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length802
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A1_MOUSE (Elongation factor 1-alpha 1 OS=Mus musculus GN=Eef1a1 PE=1 SV=3)

HSP 1 Score: 388.652 bits (997), Expect = 2.065e-107
Identity = 193/253 (76.28%), Postives = 215/253 (84.98%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTHINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAEM K SFKYAWVLDKLKAERERGITIDI+LWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RY+EIVKEVS+Y+KK+GYNP+ V FVPISG+ GDNM+E S N+ W+K            G TLL+ALD I  P RP+DKPLRLPLQDV
Sbjct:    1 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGSASGTTLLEALDCILPPTRPTDKPLRLPLQDV 253          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A1_HUMAN (Elongation factor 1-alpha 1 OS=Homo sapiens GN=EEF1A1 PE=1 SV=1)

HSP 1 Score: 388.652 bits (997), Expect = 2.065e-107
Identity = 193/253 (76.28%), Postives = 215/253 (84.98%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTHINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAEM K SFKYAWVLDKLKAERERGITIDI+LWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RY+EIVKEVS+Y+KK+GYNP+ V FVPISG+ GDNM+E S N+ W+K            G TLL+ALD I  P RP+DKPLRLPLQDV
Sbjct:    1 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDV 253          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A1_HORSE (Elongation factor 1-alpha 1 OS=Equus caballus GN=EEF1A1 PE=2 SV=1)

HSP 1 Score: 388.652 bits (997), Expect = 2.065e-107
Identity = 193/253 (76.28%), Postives = 215/253 (84.98%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTHINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAEM K SFKYAWVLDKLKAERERGITIDI+LWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RY+EIVKEVS+Y+KK+GYNP+ V FVPISG+ GDNM+E S N+ W+K            G TLL+ALD I  P RP+DKPLRLPLQDV
Sbjct:    1 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDV 253          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A1_FELCA (Elongation factor 1-alpha 1 OS=Felis catus GN=EEF1A1 PE=2 SV=1)

HSP 1 Score: 388.652 bits (997), Expect = 2.065e-107
Identity = 193/253 (76.28%), Postives = 215/253 (84.98%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTHINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAEM K SFKYAWVLDKLKAERERGITIDI+LWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RY+EIVKEVS+Y+KK+GYNP+ V FVPISG+ GDNM+E S N+ W+K            G TLL+ALD I  P RP+DKPLRLPLQDV
Sbjct:    1 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDV 253          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A1_CRIGR (Elongation factor 1-alpha 1 OS=Cricetulus griseus GN=EEF1A1 PE=2 SV=1)

HSP 1 Score: 388.652 bits (997), Expect = 2.065e-107
Identity = 193/253 (76.28%), Postives = 215/253 (84.98%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTHINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAEM K SFKYAWVLDKLKAERERGITIDI+LWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RY+EIVKEVS+Y+KK+GYNP+ V FVPISG+ GDNM+E S N+ W+K            G TLL+ALD I  P RP+DKPLRLPLQDV
Sbjct:    1 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGSASGTTLLEALDCILPPTRPTDKPLRLPLQDV 253          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A1_BOVIN (Elongation factor 1-alpha 1 OS=Bos taurus GN=EEF1A1 PE=2 SV=1)

HSP 1 Score: 388.652 bits (997), Expect = 2.065e-107
Identity = 193/253 (76.28%), Postives = 215/253 (84.98%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTHINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAEM K SFKYAWVLDKLKAERERGITIDI+LWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RY+EIVKEVS+Y+KK+GYNP+ V FVPISG+ GDNM+E S N+ W+K            G TLL+ALD I  P RP+DKPLRLPLQDV
Sbjct:    1 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDV 253          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A0_XENLA (Elongation factor 1-alpha, somatic form OS=Xenopus laevis GN=eef1as PE=2 SV=1)

HSP 1 Score: 388.652 bits (997), Expect = 2.065e-107
Identity = 193/253 (76.28%), Postives = 215/253 (84.98%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTHINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAEM K SFKYAWVLDKLKAERERGITIDI+LWKFET+KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLA+TLGVKQ+I   NKMD+T P YS+ RY+EIVKEVS+Y+KK+GYNP+ V FVPISG+ GDNM+E S N+ W+K            G TLL+ALD I  P RP+DKPLRLPLQDV
Sbjct:    1 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGINKMDSTEPPYSQKRYEEIVKEVSTYIKKIGYNPDTVAFVPISGWNGDNMLEPSPNMPWFKGWKITRKEGSGSGTTLLEALDCILPPSRPTDKPLRLPLQDV 253          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_ABSGL (Elongation factor 1-alpha OS=Absidia glauca GN=TEF-1 PE=3 SV=1)

HSP 1 Score: 388.267 bits (996), Expect = 2.697e-107
Identity = 191/253 (75.49%), Postives = 217/253 (85.77%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTH+N+VVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAE+ K SFKYAWVLDKLKAERERGITIDIALWKFET KY+ TVIDAPGHRDFIKNMITGTSQADC +LII + TG FEAGISKDGQTREHALLAFTLGV+Q+I   NKMD+T  K+S+ R++EI+KEVS ++KK+G+NP+ VPFVPISG+ GDNM+E STN+ WYK            G TLLDA+D I+ P+RPSDKPLRLPLQDV
Sbjct:    1 MGKEKTHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYHVTVIDAPGHRDFIKNMITGTSQADCGILIIAAGTGEFEAGISKDGQTREHALLAFTLGVRQLIVAINKMDST--KWSEQRFNEIIKEVSGFIKKIGFNPKSVPFVPISGWHGDNMLEESTNMPWYKGWNKETKAGAKSGKTLLDAIDAIDPPQRPSDKPLRLPLQDV 251          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_TETPY (Elongation factor 1-alpha OS=Tetrahymena pyriformis PE=2 SV=1)

HSP 1 Score: 387.882 bits (995), Expect = 3.523e-107
Identity = 187/238 (78.57%), Postives = 206/238 (86.55%), Query Frame = 3
Query:   87 EKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            +K HIN+VVIGHVDSGKSTTTGHLIYK GGIDKRVIE+FEKE+AE  K SFKYAWVLDKLKAERERGITIDI+LWKFET KY+ T+IDAPGHRDFIKNMITGTSQAD A+L+I S  G FEAGISKDGQTREHALLAFTLGVKQMI C NKMD  T  +S+ RY EI KE+S YLKKVGY P+ +PF+PISGF GDNM+ERSTN  WYKGP L++ALD +  PKRP DKPLRLPLQDV
Sbjct:    5 DKVHINLVVIGHVDSGKSTTTGHLIYKCGGIDKRVIEKFEKESAEQGKGSFKYAWVLDKLKAERERGITIDISLWKFETAKYHFTIIDAPGHRDFIKNMITGTSQADVAILMIASPQGEFEAGISKDGQTREHALLAFTLGVKQMIVCLNKMDEKTVNFSEERYQEIKKELSDYLKKVGYKPDTIPFIPISGFNGDNMLERSTNAPWYKGPILVEALDALEPPKRPVDKPLRLPLQDV 242          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A4_OSCTI (Elongation factor 1-alpha 4 OS=Oscheius tipulae GN=eft-4 PE=3 SV=1)

HSP 1 Score: 387.882 bits (995), Expect = 3.523e-107
Identity = 190/253 (75.10%), Postives = 212/253 (83.79%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTHINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVLDKLKAERERGITIDIALWKFET K+Y T+IDAPGHRDFIKNMITGTSQADCAVL++   TG FEAGISK+GQTREHALLA TLGVKQMI  CNKMD+T P +S+ R++EI+ EV S++KK+GYNP  +PFVPISGF GDNM+E S N+ WYK            G TLL+ALD I  P+RP+D+PLRLPLQDV
Sbjct:    1 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKFYVTIIDAPGHRDFIKNMITGTSQADCAVLVVACGTGEFEAGISKNGQTREHALLAQTLGVKQMIVACNKMDSTEPPFSEKRFEEIITEVKSFIKKIGYNPATIPFVPISGFNGDNMLEPSANMSWYKGWSVERKEGNASGKTLLEALDCIIPPQRPTDRPLRLPLQDV 253          
The following BLAST results are available for this feature:
BLAST of CN186363 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
EF1A1_MOUSE2.065e-10776.28Elongation factor 1-alpha 1 OS=Mus musculus GN=Eef... [more]
EF1A1_HUMAN2.065e-10776.28Elongation factor 1-alpha 1 OS=Homo sapiens GN=EEF... [more]
EF1A1_HORSE2.065e-10776.28Elongation factor 1-alpha 1 OS=Equus caballus GN=E... [more]
EF1A1_FELCA2.065e-10776.28Elongation factor 1-alpha 1 OS=Felis catus GN=EEF1... [more]
EF1A1_CRIGR2.065e-10776.28Elongation factor 1-alpha 1 OS=Cricetulus griseus ... [more]
EF1A1_BOVIN2.065e-10776.28Elongation factor 1-alpha 1 OS=Bos taurus GN=EEF1A... [more]
EF1A0_XENLA2.065e-10776.28Elongation factor 1-alpha, somatic form OS=Xenopus... [more]
EF1A_ABSGL2.697e-10775.49Elongation factor 1-alpha OS=Absidia glauca GN=TEF... [more]
EF1A_TETPY3.523e-10778.57Elongation factor 1-alpha OS=Tetrahymena pyriformi... [more]
EF1A4_OSCTI3.523e-10775.10Elongation factor 1-alpha 4 OS=Oscheius tipulae GN... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0002M13_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0002M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN186363 ID=CN186363; Name=CN186363; organism=Citrus sinensis; type=EST; length=802bp
GCGGTTTTCCGAAGGGCGGCGAGAGAGAACCATTTTCGCGAATATCATTT
TAATAAGGTTTTTTCTAGATTTGCCTAATGGGTAAGGAGAAGACTCACAT
CAACATTGTGGTCATCGGCCATGTCGACTCTGGAAAGTCGACCACCACCG
GACACTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATCGAGAGG
TTCGAGAAGGAAGCTGCTGAGATGAACAAGAGGTCATTCAAATACGCCTG
GGTGCTCGACAAGCTCAAGGCTGAGCGTGAACGTGGTATTACCATTGATA
TTGCCTTGTGGAAGTTCGAGACCACCAAGTACTACTGCACCGTCATTGAT
GCTCCCGGACATCGTGACTTTATCAAGAACATGATTACCGGTACCTCACA
GGCTGACTGTGCTGTCCTCATCATTGACTCTACCACTGGTGGTTTTGAAG
CTGGTATCTCCAAGGATGGTCAGACTCGTGAGCATGCTTTGCTTGCTTTC
ACCCTTGGTGTGAAGCAGATGATTTGCTGCTGTAACAAGATGGACGCCAC
CACCCCCAAGTATTCAAAGGCTAGGTACGATGAAATTGTGAAGGAGGTCT
CTTCCTACCTGAAGAAGGTTGGATACAACCCCGAGAAAGTCCCATTCGTG
CCCATCTCTGGTTTCGAGGGTGACAACATGATTGAGAGGTCTACCAACCT
TGACTGGTACAAGGGTCCAACCCTGCTGGACGCTCTTGACAATATCAATG
AGCCGAAGAGGCCCTCAGACAAGCCCCTTCGTCTGCCACTCCAGGACGTG
TA
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