CN186363

Overview
NameCN186363
Unique NameCN186363
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length802
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A2_XENLA (Elongation factor 1-alpha, oocyte form OS=Xenopus laevis GN=eef1ao PE=1 SV=1)

HSP 1 Score: 381.333 bits (978), Expect = 3.297e-105
Identity = 190/253 (75.10%), Postives = 212/253 (83.79%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAEM K SFKYAWVLDKLKAERERGITIDI+LWKFET K+Y T+IDAPGHRDFIKNMITGTSQADCAVLI+    G FEAGISK+GQTREHALLAFTLGVKQ+I   NKMD+T P +S+ R++EI KEVS+Y+KK+GYNP  VPFVPISG+ GDNM+E STN+ W+K            G TLL+ALD I  P+RP+ KPLRLPLQDV
Sbjct:    1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETGKFYITIIDAPGHRDFIKNMITGTSQADCAVLIVAGGVGEFEAGISKNGQTREHALLAFTLGVKQLIIGVNKMDSTEPPFSQKRFEEITKEVSAYIKKIGYNPATVPFVPISGWHGDNMLEASTNMPWFKGWKIERKEGNASGVTLLEALDCIIPPQRPTAKPLRLPLQDV 253          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A2_SCHPO (Elongation factor 1-alpha-B/C OS=Schizosaccharomyces pombe GN=tef1b PE=1 SV=1)

HSP 1 Score: 381.333 bits (978), Expect = 3.297e-105
Identity = 190/253 (75.10%), Postives = 213/253 (84.19%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWY------------KGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEK HIN+VVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA E+ K SFKYAWVLDKLKAERERGITIDIALWKFET KY  TVIDAPGHRDFIKNMITGTSQADCA+LII   TG FEAGISKDGQTREHALLA+TLGVKQ+I   NKMD T   +S+AR++EIVKE S+++KKVG+NP+ VPFVP+SGF+GDNMIE +TN+ WY            KG TLL+A+D+I  P RP+DKPLRLPLQDV
Sbjct:    1 MGKEKGHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEATELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIGGGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAVNKMDTT--GWSQARFEEIVKETSNFIKKVGFNPKTVPFVPVSGFQGDNMIEPTTNMPWYQGWQKETKAGVVKGKTLLEAIDSIEPPARPTDKPLRLPLQDV 251          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A2_RHIRA (Elongation factor 1-alpha OS=Rhizomucor racemosus GN=TEF-2 PE=3 SV=1)

HSP 1 Score: 381.333 bits (978), Expect = 3.297e-105
Identity = 190/253 (75.10%), Postives = 212/253 (83.79%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTH+N+VVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAE+ K SFKYAWVLDKLKAERERGITIDIALWKFET KY  TVIDAPGHRDFIKNMITGTSQADCA+LII   TG FEAGISKDGQTREHALLAFTLG +Q+I   NKMD T  K+S+ RY+EIVKEVS ++KK+G+NP+ VPFVPISG+ GDNM++ STN+ W+K            G TLL+A+D I  P RPSDKPLRLPLQDV
Sbjct:    1 MGKEKTHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGTGEFEAGISKDGQTREHALLAFTLGFRQLIVAINKMDTT--KWSQDRYNEIVKEVSGFIKKIGFNPKSVPFVPISGWHGDNMLDESTNMPWFKGWNKETKAGSKTGKTLLEAIDAIEPPVRPSDKPLRLPLQDV 251          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_SORMA (Elongation factor 1-alpha OS=Sordaria macrospora GN=TEF PE=3 SV=1)

HSP 1 Score: 380.948 bits (977), Expect = 4.306e-105
Identity = 192/254 (75.59%), Postives = 213/254 (83.86%), Query Frame = 3
Query:   78 MGKE-KTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKE K HIN+VVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAE+ K SFKYAWVLDKLKAERERGITIDIALWKFET KYY TVIDAPGHRDFIKNMITGTSQADCA+LII + TG FEAGISKDGQTREHALLA+TLGVKQ+I   NKMD T  ++S+AR++EI+KE  +++KKVGYNP  V FVPISGF GDNM+E STN  WYK            G TLL+A+D I +PKRP+DKPLRLPLQDV
Sbjct:    1 MGKEDKAHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYYVTVIDAPGHRDFIKNMITGTSQADCAILIIAAGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAINKMDTT--QWSQARFEEIIKETKNFIKKVGYNPATVAFVPISGFNGDNMLEASTNCPWYKGWEKETKAGKSTGKTLLEAIDAIEQPKRPTDKPLRLPLQDV 252          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_AJECG (Elongation factor 1-alpha OS=Ajellomyces capsulata (strain ATCC 26029 / G186AR / H82 / RMSCC 2432) GN=TEF PE=2 SV=1)

HSP 1 Score: 380.563 bits (976), Expect = 5.624e-105
Identity = 193/254 (75.98%), Postives = 214/254 (84.25%), Query Frame = 3
Query:   78 MGKE-KTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKE KTHIN+VVIGHVDSGKSTTTGHLIYK GGID R IE+FEKEA E+ K+SFKYAWVLDKLK+ERERGITIDIALWKFET KY  TVIDAPGHRDFIKNMITGTSQADCA+LII + TG FEAGISKDGQTREHALLAFTLGV+Q+I   NKMD T  K+S++R++EI+KEVS+++KKVGYNP+ VPFVPISGFEGDNMIE S N  WYK            G TLLDA+D I  P RP+DKPLRLPLQDV
Sbjct:    1 MGKEDKTHINLVVIGHVDSGKSTTTGHLIYKCGGIDSRTIEKFEKEAEELGKKSFKYAWVLDKLKSERERGITIDIALWKFETPKYSVTVIDAPGHRDFIKNMITGTSQADCAILIIAAGTGEFEAGISKDGQTREHALLAFTLGVRQLIVAINKMDTT--KWSESRFNEIIKEVSNFIKKVGYNPKAVPFVPISGFEGDNMIEPSPNCTWYKGWNKETASGKSSGKTLLDAIDAIEPPTRPTDKPLRLPLQDV 252          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A1_RHIRA (Elongation factor 1-alpha OS=Rhizomucor racemosus GN=TEF-1 PE=3 SV=1)

HSP 1 Score: 380.563 bits (976), Expect = 5.624e-105
Identity = 190/253 (75.10%), Postives = 211/253 (83.40%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTH+N+VVIGHVDSGKSTTTGHLIYK GGIDKR IE FEKEAAE+ K SFKYAWVLDKLKAERERGITIDIALWKFET KY  TVIDAPGHRDFIKNMITGTSQADCA+LII   TG FEAGISKDGQTREHALLAFTLG +Q+I   NKMD T  K+S+ RY+EIVKEVS ++KK+G+NP+ VPFVPISG+ GDNM++ STN+ W+K            G TLL+A+D I  P RPSDKPLRLPLQDV
Sbjct:    1 MGKEKTHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEEFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYNVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGTGEFEAGISKDGQTREHALLAFTLGFRQLIVAINKMDTT--KWSQDRYNEIVKEVSGFIKKIGFNPKSVPFVPISGWHGDNMLDESTNMPWFKGWNKETKAGSKTGKTLLEAIDAIEPPVRPSDKPLRLPLQDV 251          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_ARXAD (Elongation factor 1-alpha OS=Arxula adeninivorans GN=TEF PE=3 SV=1)

HSP 1 Score: 380.178 bits (975), Expect = 7.346e-105
Identity = 190/253 (75.10%), Postives = 212/253 (83.79%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTH+N+VVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAE+ K SFKYAWVLDKLKAERER ITIDIALWKFET KYY TVIDAPGHRDFIKNMITGTSQADCA+LII   TG FEAGISKDGQTREHALLAFTLGV+Q+I   NKMD+    +S+ RY+EIVKE S+++KKVG+NP+ VPFVPISG+ GDNMIE +TN  WYK            G TLL+A+D ++ P RPSDKPLRLPLQDV
Sbjct:    1 MGKEKTHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERVITIDIALWKFETPKYYVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGTGEFEAGISKDGQTREHALLAFTLGVRQLIVAINKMDSV--NWSEDRYNEIVKETSNFIKKVGFNPKAVPFVPISGWNGDNMIEATTNASWYKGWHKETKEGKATGKTLLEAIDAVDPPTRPSDKPLRLPLQDV 251          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A1_EUPCR (Elongation factor 1-alpha 1 OS=Euplotes crassus GN=EFA1 PE=3 SV=1)

HSP 1 Score: 379.793 bits (974), Expect = 9.594e-105
Identity = 186/241 (77.18%), Postives = 207/241 (85.89%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEK H+N+VVIGHVDSGKSTTTGHLIYKLGGID R IE+FEKE+AEM K SFKYAWVLDKLKAERERGITIDIALWKFET   + T+IDAPGHRDFIKNMITGTSQAD A+LII S TG FEAGISK+GQTREHALLA+T+GVKQM+   NKMD+T P YS+ RY+EI KEVS++L KVGY P K+ FVPISGF+GDN+ E STN+ WYKGPTL  ALD+   PKRP  KPLRLPLQDV
Sbjct:    1 MGKEKEHLNLVVIGHVDSGKSTTTGHLIYKLGGIDARTIEKFEKESAEMGKASFKYAWVLDKLKAERERGITIDIALWKFETENRHYTIIDAPGHRDFIKNMITGTSQADAAILIIASGTGEFEAGISKEGQTREHALLAYTMGVKQMVVAMNKMDSTEPPYSEDRYEEIKKEVSTFLAKVGYKPAKMNFVPISGFQGDNIQENSTNMPWYKGPTLCAALDSFKIPKRPIAKPLRLPLQDV 241          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_SCHCO (Elongation factor 1-alpha OS=Schizophyllum commune GN=TEF1 PE=3 SV=1)

HSP 1 Score: 379.407 bits (973), Expect = 1.253e-104
Identity = 191/253 (75.49%), Postives = 210/253 (83.00%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWY------------KGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEK H+N+VVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAE+ K SFKYAWVLDKLKAERERGITIDIALWKFET KY  TVIDAPGHRDFIKNMITGTSQADCA+L I   TG FEAGISKDGQTREHALLAFTLGV+Q+I   NKMD T  K+S+ R++EIVKE S+++KKVGYNP+ V FVPISG+ GDNM+E STN+ WY            KG TLLDA+D I  P RPSDKPLRLPLQDV
Sbjct:    1 MGKEKLHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYMVTVIDAPGHRDFIKNMITGTSQADCAILTIAGGTGEFEAGISKDGQTREHALLAFTLGVRQLIVAVNKMDTT--KWSEDRFNEIVKETSTFIKKVGYNPKTVAFVPISGWHGDNMLEESTNMPWYKGWTKETKAGVVKGKTLLDAIDAIEPPVRPSDKPLRLPLQDV 251          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_NEUCR (Elongation factor 1-alpha OS=Neurospora crassa GN=tef-1 PE=3 SV=2)

HSP 1 Score: 379.407 bits (973), Expect = 1.253e-104
Identity = 192/254 (75.59%), Postives = 212/254 (83.46%), Query Frame = 3
Query:   78 MGKE-KTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKE KTHIN+VVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAE+ K SFKYAWVLDKLKAERERGITIDIALWKFET KYY TVIDAPGHRDFIKNMITGTSQADCA+LII + TG FEAGISKDGQTREHALLA+TLGVKQ+I   NKMD T  ++S+ R++EI+KE  +++KKVGYNP  V FVPISGF GDNM+E STN  WYK            G TLL+A+D I  PKRP+DKPLRLPLQDV
Sbjct:    1 MGKEDKTHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYYVTVIDAPGHRDFIKNMITGTSQADCAILIIAAGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAINKMDTT--QWSQTRFEEIIKETKNFIKKVGYNPAGVAFVPISGFNGDNMLEPSTNCPWYKGWEKETKAGKATGKTLLEAIDAIEPPKRPTDKPLRLPLQDV 252          
The following BLAST results are available for this feature:
BLAST of CN186363 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
EF1A2_XENLA3.297e-10575.10Elongation factor 1-alpha, oocyte form OS=Xenopus ... [more]
EF1A2_SCHPO3.297e-10575.10Elongation factor 1-alpha-B/C OS=Schizosaccharomyc... [more]
EF1A2_RHIRA3.297e-10575.10Elongation factor 1-alpha OS=Rhizomucor racemosus ... [more]
EF1A_SORMA4.306e-10575.59Elongation factor 1-alpha OS=Sordaria macrospora G... [more]
EF1A_AJECG5.624e-10575.98Elongation factor 1-alpha OS=Ajellomyces capsulata... [more]
EF1A1_RHIRA5.624e-10575.10Elongation factor 1-alpha OS=Rhizomucor racemosus ... [more]
EF1A_ARXAD7.346e-10575.10Elongation factor 1-alpha OS=Arxula adeninivorans ... [more]
EF1A1_EUPCR9.594e-10577.18Elongation factor 1-alpha 1 OS=Euplotes crassus GN... [more]
EF1A_SCHCO1.253e-10475.49Elongation factor 1-alpha OS=Schizophyllum commune... [more]
EF1A_NEUCR1.253e-10475.59Elongation factor 1-alpha OS=Neurospora crassa GN=... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0002M13_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0002M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN186363 ID=CN186363; Name=CN186363; organism=Citrus sinensis; type=EST; length=802bp
GCGGTTTTCCGAAGGGCGGCGAGAGAGAACCATTTTCGCGAATATCATTT
TAATAAGGTTTTTTCTAGATTTGCCTAATGGGTAAGGAGAAGACTCACAT
CAACATTGTGGTCATCGGCCATGTCGACTCTGGAAAGTCGACCACCACCG
GACACTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATCGAGAGG
TTCGAGAAGGAAGCTGCTGAGATGAACAAGAGGTCATTCAAATACGCCTG
GGTGCTCGACAAGCTCAAGGCTGAGCGTGAACGTGGTATTACCATTGATA
TTGCCTTGTGGAAGTTCGAGACCACCAAGTACTACTGCACCGTCATTGAT
GCTCCCGGACATCGTGACTTTATCAAGAACATGATTACCGGTACCTCACA
GGCTGACTGTGCTGTCCTCATCATTGACTCTACCACTGGTGGTTTTGAAG
CTGGTATCTCCAAGGATGGTCAGACTCGTGAGCATGCTTTGCTTGCTTTC
ACCCTTGGTGTGAAGCAGATGATTTGCTGCTGTAACAAGATGGACGCCAC
CACCCCCAAGTATTCAAAGGCTAGGTACGATGAAATTGTGAAGGAGGTCT
CTTCCTACCTGAAGAAGGTTGGATACAACCCCGAGAAAGTCCCATTCGTG
CCCATCTCTGGTTTCGAGGGTGACAACATGATTGAGAGGTCTACCAACCT
TGACTGGTACAAGGGTCCAACCCTGCTGGACGCTCTTGACAATATCAATG
AGCCGAAGAGGCCCTCAGACAAGCCCCTTCGTCTGCCACTCCAGGACGTG
TA
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