CN186363

Overview
NameCN186363
Unique NameCN186363
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length802
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_YEAST (Elongation factor 1-alpha OS=Saccharomyces cerevisiae GN=TEF1 PE=1 SV=1)

HSP 1 Score: 378.637 bits (971), Expect = 2.137e-104
Identity = 189/253 (74.70%), Postives = 211/253 (83.40%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWY------------KGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEK+HIN+VVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAE+ K SFKYAWVLDKLKAERERGITIDIALWKFET KY  TVIDAPGHRDFIKNMITGTSQADCA+LII    G FEAGISKDGQTREHALLAFTLGV+Q+I   NKMD+   K+ ++R+ EIVKE S+++KKVGYNP+ VPFVPISG+ GDNMIE +TN  WY            KG TLL+A+D I +P RP+DKPLRLPLQDV
Sbjct:    1 MGKEKSHINVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYQVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGVGEFEAGISKDGQTREHALLAFTLGVRQLIVAVNKMDSV--KWDESRFQEIVKETSNFIKKVGYNPKTVPFVPISGWNGDNMIEATTNAPWYKGWEKETKAGVVKGKTLLEAIDAIEQPSRPTDKPLRLPLQDV 251          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_TRIRE (Elongation factor 1-alpha OS=Trichoderma reesei GN=tef1 PE=3 SV=1)

HSP 1 Score: 378.252 bits (970), Expect = 2.791e-104
Identity = 191/254 (75.20%), Postives = 213/254 (83.86%), Query Frame = 3
Query:   78 MGKE-KTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKE KTHIN+VVIGHVDSGKSTTTGHLIY+ GGIDKR IE+FEKEAAE+ K SFKYAWVLDKLKAERERGITIDIALWKFET KYY TVIDAPGHRDFIKNMITGTSQADCA+LII + TG FEAGISKDGQTREHALLA+TLGVKQ+I   NKMD  T  +++ARY EI+KE S+++KKVG+NP+ V FVPISGF GDNM+  STN  WYK            G TLL+A+D+I  PKRP+DKPLRLPLQDV
Sbjct:    1 MGKEDKTHINVVVIGHVDSGKSTTTGHLIYQCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYYVTVIDAPGHRDFIKNMITGTSQADCAILIIAAGTGEFEAGISKDGQTREHALLAYTLGVKQLIVAINKMD--TANWAEARYQEIIKETSNFIKKVGFNPKAVAFVPISGFNGDNMLTPSTNCPWYKGWEKETKAGKFTGKTLLEAIDSIEPPKRPTDKPLRLPLQDV 252          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_PUCGR (Elongation factor 1-alpha OS=Puccinia graminis GN=TEF PE=3 SV=2)

HSP 1 Score: 378.252 bits (970), Expect = 2.791e-104
Identity = 190/253 (75.10%), Postives = 212/253 (83.79%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWY------------KGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEK H+N+VVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAE+ K SFKYAWVLDKLKAERERGITIDIALWKFET KYY TVIDAPGHRDFIKNMITGTSQADCA+LII + TG FEAGISKDGQTREHALLAFTLGV+Q+I   NKMD T  K+S+ R+ EIVKE S+++KKVGYNP+ + FVPISG+ GDNM+E STN+ W+            KG TLLDA+D I  P RP+DKPLRLPLQDV
Sbjct:    1 MGKEKNHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYYVTVIDAPGHRDFIKNMITGTSQADCAILIIAAGTGEFEAGISKDGQTREHALLAFTLGVRQLIVAINKMDTT--KWSEQRF-EIVKETSNFVKKVGYNPKSIAFVPISGWHGDNMLEESTNMGWFKGWTKETKAGVSKGKTLLDAIDAIEPPSRPTDKPLRLPLQDV 250          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_ASPOR (Elongation factor 1-alpha OS=Aspergillus oryzae GN=tef1 PE=3 SV=1)

HSP 1 Score: 377.867 bits (969), Expect = 3.646e-104
Identity = 194/254 (76.38%), Postives = 213/254 (83.86%), Query Frame = 3
Query:   78 MGKE-KTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKE K HINIVVIGHVDSGKSTTTGHLIYK GGID+R IE+FEKEAAE+ K SFKYAWVLDKLK+ERERGITIDIALWKF+T+KY  TVIDAPGHRDFIKNMITGTSQADCA+LII S TG FEAGISKDGQTREHALLAFTLGV+Q+I   NKMD  T K+S+ RY+EIVKE S+++KKVGYNP+ VPFVPISGF GDNMIE STN  WYK            G TLL+A+D I  P RP+DKPLRLPLQDV
Sbjct:    1 MGKEDKQHINIVVIGHVDSGKSTTTGHLIYKCGGIDQRTIEKFEKEAAELGKGSFKYAWVLDKLKSERERGITIDIALWKFQTSKYEVTVIDAPGHRDFIKNMITGTSQADCAILIIASGTGEFEAGISKDGQTREHALLAFTLGVRQLIVALNKMD--TCKWSQDRYNEIVKETSNFIKKVGYNPKSVPFVPISGFNGDNMIEASTNCPWYKGWEKETKAGKSTGKTLLEAIDAIEPPVRPTDKPLRLPLQDV 252          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_ASHGO (Elongation factor 1-alpha OS=Ashbya gossypii GN=TEF PE=3 SV=1)

HSP 1 Score: 377.481 bits (968), Expect = 4.761e-104
Identity = 189/253 (74.70%), Postives = 210/253 (83.00%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWY------------KGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTH+N+VVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAE+ K SFKYAWVLDKLKAERERGITIDIALWKFET KY+ TVIDAPGHRDFIKNMITGTSQADCA+LII    G FEAGISKDGQTREHALLA+TLGVKQ+I   NKMD+   K+ ++RY EIVKE S+++KKVGYNP+ VPFVPISG+ GDNMIE +TN  WY            KG TLL+A+D I  P RP+DK LRLPLQDV
Sbjct:    1 MGKEKTHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKFETPKYHVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGVGEFEAGISKDGQTREHALLAYTLGVKQLIVAINKMDSV--KWDESRYQEIVKETSNFIKKVGYNPKTVPFVPISGWNGDNMIEATTNAPWYKGWEKETKAGAVKGKTLLEAIDAIEPPVRPTDKALRLPLQDV 251          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_HYDAT (Elongation factor 1-alpha OS=Hydra attenuata PE=2 SV=1)

HSP 1 Score: 377.096 bits (967), Expect = 6.219e-104
Identity = 189/255 (74.12%), Postives = 211/255 (82.75%), Query Frame = 3
Query:   75 LMGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK-------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            +  K K HINIVVIGHVDSGKST+TGH+IYK GGIDKR IE+FEKEA EM K SFKYAWVLDKLKAERERGITIDIALWKFETTKY  T+IDAPGHRDFIKNMITGTSQADCAVLI+ S+TG FEAGISK+GQTREHALLAFTLGVKQMI   NK+D T P YS+AR++EI KE+S+Y+KKVGY+P+ VP +P+SG+ GDNMIE S N+ WYK             G TLL+ALDNI  P RPS KPLRLPLQDV
Sbjct:    1 MSNKVKPHINIVVIGHVDSGKSTSTGHMIYKCGGIDKRQIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETTKYVVTIIDAPGHRDFIKNMITGTSQADCAVLIVASSTGEFEAGISKNGQTREHALLAFTLGVKQMIVAVNKIDNTEPPYSEARFNEIKKEISAYVKKVGYDPKTVPVLPVSGWHGDNMIEPSPNMSWYKGWEVEYKDTGKHTGKTLLEALDNIPLPARPSSKPLRLPLQDV 255          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_PIRIN (Elongation factor 1-alpha OS=Piriformospora indica GN=TEF1 PE=2 SV=1)

HSP 1 Score: 376.326 bits (965), Expect = 1.061e-103
Identity = 190/255 (74.51%), Postives = 210/255 (82.35%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK--------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEK H+N+VVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAE+ K SFKYAWVLDKLKAERERGITIDIALWK ET KY  TVIDAPGHRDFIKNMITGTSQADCA+LII   TG FEAGISKDGQTREHALLAFTLGV+Q+I   NKMD T   +S+AR++EIVKE S+++KKVGYNP+ V FVPISG+ GDNM+E STN+ WYK              G TL+DA+D I  P RPSDKPLRLPLQDV
Sbjct:    1 MGKEKAHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAELGKGSFKYAWVLDKLKAERERGITIDIALWKSETPKYMVTVIDAPGHRDFIKNMITGTSQADCAILIIAGGTGEFEAGISKDGQTREHALLAFTLGVRQLIVAVNKMDTT--NWSEARFNEIVKETSNFIKKVGYNPKTVAFVPISGWHGDNMLEPSTNMPWYKGWSKEVKGSSSPATGKTLVDAIDAIEPPVRPSDKPLRLPLQDV 253          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_CRYNE (Elongation factor 1-alpha OS=Cryptococcus neoformans GN=TEF1 PE=2 SV=2)

HSP 1 Score: 376.326 bits (965), Expect = 1.061e-103
Identity = 186/253 (73.52%), Postives = 211/253 (83.40%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWY------------KGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGK+K H+N+VVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA E+ K SFKYAWVLDKLKAERERGITIDIALWKFET +Y  TVIDAPGHRDFIKNMITGTSQADCA+LII +  G FEAGISKDGQTREHALLAFTLGV+Q+I  CNKMD  T K+S+ R++EIVKE + ++KKVGYNP+ VPFVPISG+ GDNM+E +TN+ WY            KG TLL+A+D I  P RP+DKPLRLPLQDV
Sbjct:    1 MGKDKLHVNVVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQELGKSSFKYAWVLDKLKAERERGITIDIALWKFETPRYQVTVIDAPGHRDFIKNMITGTSQADCAILIIATGIGEFEAGISKDGQTREHALLAFTLGVRQLIVACNKMD--TCKWSEDRFNEIVKETNGFIKKVGYNPKAVPFVPISGWHGDNMLEETTNMPWYKGWTKETKSGVSKGKTLLEAIDAIEPPTRPTDKPLRLPLQDV 251          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_APIME (Elongation factor 1-alpha OS=Apis mellifera PE=3 SV=1)

HSP 1 Score: 375.17 bits (962), Expect = 2.363e-103
Identity = 190/253 (75.10%), Postives = 206/253 (81.42%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEK HINIVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVLDKLKAERERGITIDIALWKFET KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ +  G FEAGISK+GQTREHALLAFTLGVKQ+I   NKMD T P YS+AR++EI KEVSSY+KK+GYN   V FVPISG+ GDNM+E S    WYK            G TL++ALD I  P RP+DK LRLPLQDV
Sbjct:    1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKIGYNTASVAFVPISGWHGDNMLEPSPKTPWYKGWKVERKDGNADGKTLIEALDAILPPSRPTDKALRLPLQDV 253          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_CRYPV (Elongation factor 1-alpha OS=Cryptosporidium parvum PE=2 SV=1)

HSP 1 Score: 369.777 bits (948), Expect = 9.928e-102
Identity = 183/241 (75.93%), Postives = 206/241 (85.48%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTHIN+VVIGHVDSGKSTTTGHLIYKLGGIDKR IE+FEKE++EM K SFKYAWVLDKLKAERERGITIDIALW+FET KY+ TVIDAPGHRDFIKNMITGTSQAD A+L++ +    FE   SK+GQTREHALLAFTLGV+QMI   NKMD  T +Y ++R+DEI  EV  YLKKVGYN EK+PFV ISGF GDNM+ERS  + WYKG TL++ALD +  PKRP+DKPLRLPLQDV
Sbjct:    1 MGKEKTHINLVVIGHVDSGKSTTTGHLIYKLGGIDKRTIEKFEKESSEMGKGSFKYAWVLDKLKAERERGITIDIALWQFETPKYHYTVIDAPGHRDFIKNMITGTSQADVALLVVPADR--FEGAFSKEGQTREHALLAFTLGVRQMIVGINKMD--TCEYKQSRFDEIFNEVDGYLKKVGYNTEKIPFVAISGFVGDNMVERSDKMPWYKGKTLVEALDTMEPPKRPTDKPLRLPLQDV 237          
The following BLAST results are available for this feature:
BLAST of CN186363 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
EF1A_YEAST2.137e-10474.70Elongation factor 1-alpha OS=Saccharomyces cerevis... [more]
EF1A_TRIRE2.791e-10475.20Elongation factor 1-alpha OS=Trichoderma reesei GN... [more]
EF1A_PUCGR2.791e-10475.10Elongation factor 1-alpha OS=Puccinia graminis GN=... [more]
EF1A_ASPOR3.646e-10476.38Elongation factor 1-alpha OS=Aspergillus oryzae GN... [more]
EF1A_ASHGO4.761e-10474.70Elongation factor 1-alpha OS=Ashbya gossypii GN=TE... [more]
EF1A_HYDAT6.219e-10474.12Elongation factor 1-alpha OS=Hydra attenuata PE=2 ... [more]
EF1A_PIRIN1.061e-10374.51Elongation factor 1-alpha OS=Piriformospora indica... [more]
EF1A_CRYNE1.061e-10373.52Elongation factor 1-alpha OS=Cryptococcus neoforma... [more]
EF1A_APIME2.363e-10375.10Elongation factor 1-alpha OS=Apis mellifera PE=3 S... [more]
EF1A_CRYPV9.928e-10275.93Elongation factor 1-alpha OS=Cryptosporidium parvu... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0002M13_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0002M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN186363 ID=CN186363; Name=CN186363; organism=Citrus sinensis; type=EST; length=802bp
GCGGTTTTCCGAAGGGCGGCGAGAGAGAACCATTTTCGCGAATATCATTT
TAATAAGGTTTTTTCTAGATTTGCCTAATGGGTAAGGAGAAGACTCACAT
CAACATTGTGGTCATCGGCCATGTCGACTCTGGAAAGTCGACCACCACCG
GACACTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATCGAGAGG
TTCGAGAAGGAAGCTGCTGAGATGAACAAGAGGTCATTCAAATACGCCTG
GGTGCTCGACAAGCTCAAGGCTGAGCGTGAACGTGGTATTACCATTGATA
TTGCCTTGTGGAAGTTCGAGACCACCAAGTACTACTGCACCGTCATTGAT
GCTCCCGGACATCGTGACTTTATCAAGAACATGATTACCGGTACCTCACA
GGCTGACTGTGCTGTCCTCATCATTGACTCTACCACTGGTGGTTTTGAAG
CTGGTATCTCCAAGGATGGTCAGACTCGTGAGCATGCTTTGCTTGCTTTC
ACCCTTGGTGTGAAGCAGATGATTTGCTGCTGTAACAAGATGGACGCCAC
CACCCCCAAGTATTCAAAGGCTAGGTACGATGAAATTGTGAAGGAGGTCT
CTTCCTACCTGAAGAAGGTTGGATACAACCCCGAGAAAGTCCCATTCGTG
CCCATCTCTGGTTTCGAGGGTGACAACATGATTGAGAGGTCTACCAACCT
TGACTGGTACAAGGGTCCAACCCTGCTGGACGCTCTTGACAATATCAATG
AGCCGAAGAGGCCCTCAGACAAGCCCCTTCGTCTGCCACTCCAGGACGTG
TA
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