CN186363

Overview
NameCN186363
Unique NameCN186363
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length802
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_PLAFK (Elongation factor 1-alpha OS=Plasmodium falciparum (isolate K1 / Thailand) GN=MEF-1 PE=3 SV=1)

HSP 1 Score: 369.392 bits (947), Expect = 1.297e-101
Identity = 178/241 (73.86%), Postives = 205/241 (85.06%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEKTHIN+VVIGHVDSGKSTTTGH+IYKLGGID+R IE+FEKE+AEM K SFKYAWVLDKLKAERERGITIDIALWKFET +Y+ TVIDAPGH+DFIKNMITGTSQAD A+L++ +  GGF+   SK+GQT+EH LLAFTLGVKQ++   NKMD  T KYS+ RY+EI KEV  YLKKVGY  +KV F+PISGFEGDN+IE+S    WYKG TL++ALD +  PKRP DKPLR+PLQ V
Sbjct:    1 MGKEKTHINLVVIGHVDSGKSTTTGHIIYKLGGIDRRTIEKFEKESAEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETPRYFFTVIDAPGHKDFIKNMITGTSQADVALLVVPADVGGFDGAFSKEGQTKEHVLLAFTLGVKQIVVGVNKMD--TVKYSEDRYEEIKKEVKDYLKKVGYQADKVDFIPISGFEGDNLIEKSDKTPWYKGRTLIEALDTMQPPKRPYDKPLRIPLQGV 239          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_PORYE (Elongation factor 1-alpha OS=Porphyra yezoensis PE=2 SV=1)

HSP 1 Score: 369.007 bits (946), Expect = 1.693e-101
Identity = 183/245 (74.69%), Postives = 204/245 (83.27%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNL----DWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEK H++IVVIGHVDSGKSTTTGHLIYK GGI+KR IE+FEKEAAEM K SFKYAWVLDKLKAERERGITIDIALWKFET KY  T+IDAPGHRDFIKNMITGTSQAD A+L+I S  G FEAGIS++GQTREHALLA+TLGVKQMI  CNKMD     +S+ RY+E+ KE+  YLKKVGYNP KVP VP SG+ G+N+ ER+        WYKGP LL+ALDN + PKRP DKPLRLPLQDV
Sbjct:    1 MGKEKQHVSIVVIGHVDSGKSTTTGHLIYKCGGIEKRAIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETEKYSFTIIDAPGHRDFIKNMITGTSQADLAILVIASPPGEFEAGISQNGQTREHALLAYTLGVKQMIVACNKMDDKNVNWSQDRYEEVSKEMDLYLKKVGYNPAKVPKVPTSGWTGENLFERTDKTHALGKWYKGPCLLEALDNCDPPKRPVDKPLRLPLQDV 245          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1AC_PORPU (Elongation factor 1-alpha C OS=Porphyra purpurea GN=TEF-C PE=2 SV=1)

HSP 1 Score: 367.851 bits (943), Expect = 3.773e-101
Identity = 184/245 (75.10%), Postives = 203/245 (82.86%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTN----LDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEK H++IVVIGHVDSGKSTTTGHLIYK GGIDKR IE+FEKEAAEM K SFKYAWVLDKLKAERERGITIDIALWKFET KY  T+IDAPGHRDFIKNMITGTSQAD A+L+I S  G FEAGIS++GQTREHALLA+TLGVKQMI  CNKMD     +SK RY+E+ KE+  YLKKVGYNP KVP VP SG+ G+N+ ER+        WYKGP LL+ALD  + PKRP DKPLRLPLQDV
Sbjct:    1 MGKEKQHVSIVVIGHVDSGKSTTTGHLIYKCGGIDKRAIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETDKYNFTIIDAPGHRDFIKNMITGTSQADLAILVIASPPGEFEAGISQNGQTREHALLAYTLGVKQMIVACNKMDDKNVNWSKERYEEVSKEMDLYLKKVGYNPPKVPKVPTSGWTGENLFERTGGDHALGKWYKGPCLLEALDACDPPKRPVDKPLRLPLQDV 245          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_BLAHO (Elongation factor 1-alpha OS=Blastocystis hominis PE=2 SV=1)

HSP 1 Score: 363.614 bits (932), Expect = 7.115e-100
Identity = 179/243 (73.66%), Postives = 202/243 (83.13%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYN--PEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            MGKEK HIN+VVIGHV +GKSTTTGHLIY  GGIDKR IERFE+    + K SFKYAWVL K+KAERERGITIDI+LWKFET K + T+IDAPGHRDFIKNMITGTSQAD A+L+I S  G FEAG SK+GQTREHALLA TLGVKQMI CCNKMD  +  YS+ARY EI  E++S+L KVGY    E++PF+PISGF GDNMIE S N+ WYKGPTLL+ALDN++ PKRP DKPLRLPLQDV
Sbjct:    1 MGKEKPHINLVVIGHVVAGKSTTTGHLIYACGGIDKRTIERFEEGGQRIGKGSFKYAWVLAKMKAERERGITIDISLWKFETRKDFFTIIDAPGHRDFIKNMITGTSQADVAILVIASGAGEFEAGYSKNGQTREHALLANTLGVKQMIVCCNKMDDKSVNYSEARYKEIKNEMTSFLTKVGYAKVEERIPFIPISGFNGDNMIEHSANMPWYKGPTLLEALDNVHPPKRPVDKPLRLPLQDV 243          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_STYLE (Elongation factor 1-alpha OS=Stylonychia lemnae GN=EFAA PE=3 SV=1)

HSP 1 Score: 361.688 bits (927), Expect = 2.704e-99
Identity = 176/241 (73.03%), Postives = 201/241 (83.40%), Query Frame = 3
Query:   78 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYKGPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            M KEK H+N+VVIGHVDSGKST+TGHLIYK GGIDKR IE+FEKEAAEM K SFKYAWVLDKLKAERERGITIDIALW FET K   T+IDAPGHRDFIKNMITGTSQAD A+LII S  G FEAGISK+GQTREHALLAFT+GVKQMI   NKMD  +  + + R+ EI KE+S YLKK+   P + PF+PISG+ GDNM+E+S N+ W+ G TL+DALD +++PKRP DKPLRLPLQDV
Sbjct:    1 MPKEKNHLNLVVIGHVDSGKSTSTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDIALWNFETAKSVFTIIDAPGHRDFIKNMITGTSQADAAILIIASGQGEFEAGISKEGQTREHALLAFTMGVKQMIVAVNKMDDKSVNWDQGRFIEIKKELSDYLKKIWLQPRQDPFIPISGWHGDNMLEKSPNMPWFTGSTLIDALDALDQPKRPKDKPLRLPLQDV 241          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_SPOFR (Elongation factor 1-alpha (Fragment) OS=Spodoptera frugiperda PE=1 SV=1)

HSP 1 Score: 357.451 bits (916), Expect = 5.099e-98
Identity = 178/239 (74.48%), Postives = 198/239 (82.85%), Query Frame = 3
Query:  120 HVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            HVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVLDKLKAERERGITIDIALWKFET KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+GQTREHALLAFTLGVKQ+I   NKMD+T P YS++R++EI KEVSSY+KK+GYNP  V FVPISG+ GDNM+E ST + W+K            G  L++ALD I  P RP+DKPLRLPLQDV
Sbjct:    1 HVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEASTKMPWFKGWNVERKEGKAEGKCLIEALDAILPPARPTDKPLRLPLQDV 239          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_HELZE (Elongation factor 1-alpha (Fragment) OS=Heliothis zea PE=3 SV=1)

HSP 1 Score: 354.369 bits (908), Expect = 4.316e-97
Identity = 177/239 (74.06%), Postives = 197/239 (82.43%), Query Frame = 3
Query:  120 HVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            HVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVLDKLKAERERGITIDIALWKFET KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+GQTREHALLAFTLGVKQ+I   NKMD+T P YS++R++EI KEVSSY+KK+GYNP  V FVPISG+ GDNM+E ST + W+K            G  L++ALD I  P RP+DK LRLPLQDV
Sbjct:    1 HVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEASTKMPWFKGWNVERKEGKAEGKCLIEALDAILPPARPTDKALRLPLQDV 239          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_HELVI (Elongation factor 1-alpha (Fragment) OS=Heliothis virescens PE=3 SV=1)

HSP 1 Score: 354.369 bits (908), Expect = 4.316e-97
Identity = 177/239 (74.06%), Postives = 197/239 (82.43%), Query Frame = 3
Query:  120 HVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            HVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVLDKLKAERERGITIDIALWKFET KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+GQTREHALLAFTLGVKQ+I   NKMD+T P YS++R++EI KEVSSY+KK+GYNP  V FVPISG+ GDNM+E ST + W+K            G  L++ALD I  P RP+DK LRLPLQDV
Sbjct:    1 HVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEASTKMPWFKGWNVERKEGKAEGKCLIEALDAILPPARPTDKALRLPLQDV 239          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_HELGL (Elongation factor 1-alpha (Fragment) OS=Helicoverpa gelotopoeon PE=3 SV=1)

HSP 1 Score: 354.369 bits (908), Expect = 4.316e-97
Identity = 177/239 (74.06%), Postives = 197/239 (82.43%), Query Frame = 3
Query:  120 HVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            HVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVLDKLKAERERGITIDIALWKFET KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+GQTREHALLAFTLGVKQ+I   NKMD+T P YS++R++EI KEVSSY+KK+GYNP  V FVPISG+ GDNM+E ST + W+K            G  L++ALD I  P RP+DK LRLPLQDV
Sbjct:    1 HVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEASTKMPWFKGWNVERKEGKAEGKCLIEALDAILPPARPTDKALRLPLQDV 239          
BLAST of CN186363 vs. ExPASy Swiss-Prot
Match: EF1A_HELDI (Elongation factor 1-alpha (Fragment) OS=Heliocheilus discalis PE=3 SV=1)

HSP 1 Score: 354.369 bits (908), Expect = 4.316e-97
Identity = 177/239 (74.06%), Postives = 197/239 (82.43%), Query Frame = 3
Query:  120 HVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDKLKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGGFEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIVKEVSSYLKKVGYNPEKVPFVPISGFEGDNMIERSTNLDWYK------------GPTLLDALDNINEPKRPSDKPLRLPLQDV 800
            HVDSGKSTTTGHLIYK GGIDKR IE+FEKEA EM K SFKYAWVLDKLKAERERGITIDIALWKFET KYY T+IDAPGHRDFIKNMITGTSQADCAVLI+ + TG FEAGISK+GQTREHALLAFTLGVKQ+I   NKMD+T P YS++R++EI KEVSSY+KK+GYNP  V FVPISG+ GDNM+E ST + W+K            G  L++ALD I  P RP+DK LRLPLQDV
Sbjct:    1 HVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLDKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSESRFEEIKKEVSSYIKKIGYNPAAVAFVPISGWHGDNMLEASTKMPWFKGWNVERKEGKAEGKCLIEALDAILPPARPTDKALRLPLQDV 239          
The following BLAST results are available for this feature:
BLAST of CN186363 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
EF1A_PLAFK1.297e-10173.86Elongation factor 1-alpha OS=Plasmodium falciparum... [more]
EF1A_PORYE1.693e-10174.69Elongation factor 1-alpha OS=Porphyra yezoensis PE... [more]
EF1AC_PORPU3.773e-10175.10Elongation factor 1-alpha C OS=Porphyra purpurea G... [more]
EF1A_BLAHO7.115e-10073.66Elongation factor 1-alpha OS=Blastocystis hominis ... [more]
EF1A_STYLE2.704e-9973.03Elongation factor 1-alpha OS=Stylonychia lemnae GN... [more]
EF1A_SPOFR5.099e-9874.48Elongation factor 1-alpha (Fragment) OS=Spodoptera... [more]
EF1A_HELZE4.316e-9774.06Elongation factor 1-alpha (Fragment) OS=Heliothis ... [more]
EF1A_HELVI4.316e-9774.06Elongation factor 1-alpha (Fragment) OS=Heliothis ... [more]
EF1A_HELGL4.316e-9774.06Elongation factor 1-alpha (Fragment) OS=Helicoverp... [more]
EF1A_HELDI4.316e-9774.06Elongation factor 1-alpha (Fragment) OS=Heliocheil... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0002M13_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0002M13, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN186363 ID=CN186363; Name=CN186363; organism=Citrus sinensis; type=EST; length=802bp
GCGGTTTTCCGAAGGGCGGCGAGAGAGAACCATTTTCGCGAATATCATTT
TAATAAGGTTTTTTCTAGATTTGCCTAATGGGTAAGGAGAAGACTCACAT
CAACATTGTGGTCATCGGCCATGTCGACTCTGGAAAGTCGACCACCACCG
GACACTTGATCTACAAGCTTGGTGGTATTGACAAGCGTGTGATCGAGAGG
TTCGAGAAGGAAGCTGCTGAGATGAACAAGAGGTCATTCAAATACGCCTG
GGTGCTCGACAAGCTCAAGGCTGAGCGTGAACGTGGTATTACCATTGATA
TTGCCTTGTGGAAGTTCGAGACCACCAAGTACTACTGCACCGTCATTGAT
GCTCCCGGACATCGTGACTTTATCAAGAACATGATTACCGGTACCTCACA
GGCTGACTGTGCTGTCCTCATCATTGACTCTACCACTGGTGGTTTTGAAG
CTGGTATCTCCAAGGATGGTCAGACTCGTGAGCATGCTTTGCTTGCTTTC
ACCCTTGGTGTGAAGCAGATGATTTGCTGCTGTAACAAGATGGACGCCAC
CACCCCCAAGTATTCAAAGGCTAGGTACGATGAAATTGTGAAGGAGGTCT
CTTCCTACCTGAAGAAGGTTGGATACAACCCCGAGAAAGTCCCATTCGTG
CCCATCTCTGGTTTCGAGGGTGACAACATGATTGAGAGGTCTACCAACCT
TGACTGGTACAAGGGTCCAACCCTGCTGGACGCTCTTGACAATATCAATG
AGCCGAAGAGGCCCTCAGACAAGCCCCTTCGTCTGCCACTCCAGGACGTG
TA
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