CN186245

Overview
NameCN186245
Unique NameCN186245
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length717
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN186245 vs. ExPASy Swiss-Prot
Match: ARF1_XENLA (ADP-ribosylation factor 1 OS=Xenopus laevis GN=arf1 PE=2 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 7.861e-12
Identity = 43/131 (32.82%), Postives = 64/131 (48.85%), Query Frame = 2 
Query:  155 QKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWXXXXXXXXXXXXXXXXXXXERFAESKRELDALLSDESLANVPFLILGNKIDIPYAASEDELRYHLGL 547
            +KE +IL +GLD AGKTT+L+ LK   +V   PT     E +    I F  +D+GG    R +W                   ER  E++ EL  +L+++ L +   L+  NK D+P A +  E+   LGL
Sbjct:   15 KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGL 145
[Close]
BLAST of CN186245 vs. ExPASy Swiss-Prot
Match: ARF1_RAT (ADP-ribosylation factor 1 OS=Rattus norvegicus GN=Arf1 PE=1 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 7.861e-12
Identity = 43/131 (32.82%), Postives = 64/131 (48.85%), Query Frame = 2 
Query:  155 QKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWXXXXXXXXXXXXXXXXXXXERFAESKRELDALLSDESLANVPFLILGNKIDIPYAASEDELRYHLGL 547
            +KE +IL +GLD AGKTT+L+ LK   +V   PT     E +    I F  +D+GG    R +W                   ER  E++ EL  +L+++ L +   L+  NK D+P A +  E+   LGL
Sbjct:   15 KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGL 145
[Close]
BLAST of CN186245 vs. ExPASy Swiss-Prot
Match: ARF1_MOUSE (ADP-ribosylation factor 1 OS=Mus musculus GN=Arf1 PE=1 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 7.861e-12
Identity = 43/131 (32.82%), Postives = 64/131 (48.85%), Query Frame = 2 
Query:  155 QKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWXXXXXXXXXXXXXXXXXXXERFAESKRELDALLSDESLANVPFLILGNKIDIPYAASEDELRYHLGL 547
            +KE +IL +GLD AGKTT+L+ LK   +V   PT     E +    I F  +D+GG    R +W                   ER  E++ EL  +L+++ L +   L+  NK D+P A +  E+   LGL
Sbjct:   15 KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGL 145
[Close]
BLAST of CN186245 vs. ExPASy Swiss-Prot
Match: ARF1_MACFA (ADP-ribosylation factor 1 OS=Macaca fascicularis GN=ARF1 PE=2 SV=3)

HSP 1 Score: 70.8626 bits (172), Expect = 7.861e-12
Identity = 43/131 (32.82%), Postives = 64/131 (48.85%), Query Frame = 2 
Query:  155 QKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWXXXXXXXXXXXXXXXXXXXERFAESKRELDALLSDESLANVPFLILGNKIDIPYAASEDELRYHLGL 547
            +KE +IL +GLD AGKTT+L+ LK   +V   PT     E +    I F  +D+GG    R +W                   ER  E++ EL  +L+++ L +   L+  NK D+P A +  E+   LGL
Sbjct:   15 KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGL 145
[Close]
BLAST of CN186245 vs. ExPASy Swiss-Prot
Match: ARF1_HUMAN (ADP-ribosylation factor 1 OS=Homo sapiens GN=ARF1 PE=1 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 7.861e-12
Identity = 43/131 (32.82%), Postives = 64/131 (48.85%), Query Frame = 2 
Query:  155 QKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWXXXXXXXXXXXXXXXXXXXERFAESKRELDALLSDESLANVPFLILGNKIDIPYAASEDELRYHLGL 547
            +KE +IL +GLD AGKTT+L+ LK   +V   PT     E +    I F  +D+GG    R +W                   ER  E++ EL  +L+++ L +   L+  NK D+P A +  E+   LGL
Sbjct:   15 KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGL 145
[Close]
BLAST of CN186245 vs. ExPASy Swiss-Prot
Match: ARF1_DAUCA (ADP-ribosylation factor 1 OS=Daucus carota GN=ARF1 PE=2 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 7.861e-12
Identity = 52/177 (29.38%), Postives = 78/177 (44.07%), Query Frame = 2 
Query:  155 QKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWXXXXXXXXXXXXXXXXXXXERFAESKRELDALLSDESLANVPFLILGNKIDIPYAASEDELRYHLGLTNFTTGKGKVNLVDSNVRPLEVFMCSIVRKMGYG--EGFKWLSQYI 679
            +KE +IL +GLD AGKTT+L+ LK   +V   PT     E +    I F  +D+GG    R +W                   +R  E++ EL  +L+++ L +   L+  NK D+P A +  E+   LGL               ++R    ++ S     G G  EG  WLS  I
Sbjct:   15 KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRDRVVEARDELHRMLNEDELRDAVLLVFANKQDLPNAMNAAEIIDKLGL--------------HSLRQRHWYIQSTCATSGEGLYEGLDWLSNNI 177
[Close]
BLAST of CN186245 vs. ExPASy Swiss-Prot
Match: ARF1_BOVIN (ADP-ribosylation factor 1 OS=Bos taurus GN=ARF1 PE=1 SV=2)

HSP 1 Score: 70.8626 bits (172), Expect = 7.861e-12
Identity = 43/131 (32.82%), Postives = 64/131 (48.85%), Query Frame = 2 
Query:  155 QKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWXXXXXXXXXXXXXXXXXXXERFAESKRELDALLSDESLANVPFLILGNKIDIPYAASEDELRYHLGL 547
            +KE +IL +GLD AGKTT+L+ LK   +V   PT     E +    I F  +D+GG    R +W                   ER  E++ EL  +L+++ L +   L+  NK D+P A +  E+   LGL
Sbjct:   15 KKEMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNISFTVWDVGGQDKIRPLWRHYFQNTQGLIFVVDSNDRERVNEAREELMRMLAEDELRDAVLLVFANKQDLPNAMNAAEITDKLGL 145
[Close]
BLAST of CN186245 vs. ExPASy Swiss-Prot
Match: ARL5B_MOUSE (ADP-ribosylation factor-like protein 5B OS=Mus musculus GN=Arl5b PE=2 SV=3)

HSP 1 Score: 70.4774 bits (171), Expect = 1.027e-11
Identity = 46/174 (26.44%), Postives = 73/174 (41.95%), Query Frame = 2 
Query:  158 KEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWXXXXXXXXXXXXXXXXXXXERFAESKRELDALLSDESLANVPFLILGNKIDIPYAASEDELRYHLGLTNFTTGKGKVNLVDSNVRPLEVFMCSIVRKMGYGEGFKWLSQYI 679
            +E K++ +GLDNAGKTT+L+      +V   PT     EE+ +    F  +D+GG +  R  W                   ER A +K EL  +L+ E L     LI  NK D+    +  E+  +L L++                P  +  C  +   G  +G +W++  I
Sbjct:   15 QEHKVIIVGLDNAGKTTILYQFLMNEVVHTSPTIGSNVEEIVVKNTHFLMWDIGGQESLRSSWNTYYSNTEFIILVVDSIDRERLAITKEELYRMLAHEDLRKAAVLIFANKQDMKGCMTAAEISKYLTLSSIKD------------HPWHIQSCCALTGEGLCQGLEWMTSRI 176
[Close]
BLAST of CN186245 vs. ExPASy Swiss-Prot
Match: ARL5B_HUMAN (ADP-ribosylation factor-like protein 5B OS=Homo sapiens GN=ARL5B PE=1 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.027e-11
Identity = 46/174 (26.44%), Postives = 73/174 (41.95%), Query Frame = 2 
Query:  158 KEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWXXXXXXXXXXXXXXXXXXXERFAESKRELDALLSDESLANVPFLILGNKIDIPYAASEDELRYHLGLTNFTTGKGKVNLVDSNVRPLEVFMCSIVRKMGYGEGFKWLSQYI 679
            +E K++ +GLDNAGKTT+L+      +V   PT     EE+ +    F  +D+GG +  R  W                   ER A +K EL  +L+ E L     LI  NK D+    +  E+  +L L++                P  +  C  +   G  +G +W++  I
Sbjct:   15 QEHKVIIVGLDNAGKTTILYQFLMNEVVHTSPTIGSNVEEIVVKNTHFLMWDIGGQESLRSSWNTYYSNTEFIILVVDSIDRERLAITKEELYRMLAHEDLRKAAVLIFANKQDMKGCMTAAEISKYLTLSSIKD------------HPWHIQSCCALTGEGLCQGLEWMTSRI 176
[Close]
BLAST of CN186245 vs. ExPASy Swiss-Prot
Match: ARL5A_MOUSE (ADP-ribosylation factor-like protein 5A OS=Mus musculus GN=Arl5a PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.027e-11
Identity = 44/175 (25.14%), Postives = 73/175 (41.71%), Query Frame = 2 
Query:  158 KEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWXXXXXXXXXXXXXXXXXXXERFAESKRELDALLSDESLANVPFLILGNKIDIPYAASEDELRYHLGLTNFTTGKGKVNLVDSNVRPLEVFMCSIVRKMGYGEGFKWLSQYIK 682
            +E K++ +GLDNAGKTT+L+      +V   PT     EE+ +   +F  +D+GG +  R  W                   ER + ++ EL  +L+ E L     LI  NK D+    +  E+   L LT+    +  +              C  +   G  +G +W+   +K
Sbjct:   15 QEHKVIIVGLDNAGKTTILYQFSMNEVVHTSPTIGSNVEEIVVNNTRFLMWDIGGQESLRPSWNTYYTNTEFVIVVVDSTDRERISVTREELYKMLAHEDLRKAGLLIFANKQDVKECMTVAEISQFLKLTSIKDHQWHIQ------------ACCALTGEGLCQGLEWMMSRLK 177
The following BLAST results are available for this feature:
BLAST of CN186245 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 167
Match NameE-valueIdentityDescription
ARF1_XENLA7.861e-1232.82ADP-ribosylation factor 1 OS=Xenopus laevis GN=arf... [more]
ARF1_RAT7.861e-1232.82ADP-ribosylation factor 1 OS=Rattus norvegicus GN=... [more]
ARF1_MOUSE7.861e-1232.82ADP-ribosylation factor 1 OS=Mus musculus GN=Arf1 ... [more]
ARF1_MACFA7.861e-1232.82ADP-ribosylation factor 1 OS=Macaca fascicularis G... [more]
ARF1_HUMAN7.861e-1232.82ADP-ribosylation factor 1 OS=Homo sapiens GN=ARF1 ... [more]
ARF1_DAUCA7.861e-1229.38ADP-ribosylation factor 1 OS=Daucus carota GN=ARF1... [more]
ARF1_BOVIN7.861e-1232.82ADP-ribosylation factor 1 OS=Bos taurus GN=ARF1 PE... [more]
ARL5B_MOUSE1.027e-1126.44ADP-ribosylation factor-like protein 5B OS=Mus mus... [more]
ARL5B_HUMAN1.027e-1126.44ADP-ribosylation factor-like protein 5B OS=Homo sa... [more]
ARL5A_MOUSE1.027e-1125.14ADP-ribosylation factor-like protein 5A OS=Mus mus... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS05_0002J15_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0002J15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN186245 ID=CN186245; Name=CN186245; organism=Citrus sinensis; type=EST; length=717bp
GAGGGTTGATTCCTCCTAATTATCTCAAAGATTCATCATATTTGAGCCAA
AATTCGATCTTTTTGATAGATTCATTATAAATAAGGAGTAATAAAAGAAA
CCCATGTTTTTGTTTGATTGGTTTTATGGGATTCTTGCATCTCTTGGGCT
CTGGCAAAAAGAGGCCAAGATCTTGTTTCTCGGCCTCGATAATGCCGGTA
AAACGACGCTGCTTCATATGTTGAAAGATGAGAGATTAGTTCAACATCAA
CCAACACAACATCCCACTTCGGAGGAACTGAGTATTGGGAAAATCAAGTT
TAAGGCTTTTGATTTGGGAGGGCATCAGATTGCTCGTAGAGTTTGGAAAG
ATTACTACGCCAAGGTTGATGCGGTTGTGTACCTGGTAGATGCGTATGAC
AAGGAGAGGTTTGCTGAGTCAAAAAGGGAGCTTGATGCCCTGCTTTCAGA
TGAATCCTTGGCCAATGTTCCATTCCTAATTTTGGGCAACAAAATTGATA
TCCCATATGCTGCATCAGAAGATGAGTTGCGATATCACCTTGGACTGACC
AACTTCACCACTGGCAAGGGTAAGGTGAATCTGGTTGATTCAAACGTTCG
TCCCCTTGAGGTATTCATGTGCAGCATTGTCCGCAAAATGGGTTACGGAG
AGGGATTTAAGTGGCTGTCTCAGTACATCAAGTAGAGCAAGAAGTAGAAT
TTGTTCGGTTCTTTCTT
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