EL492485

Overview
NameEL492485
Unique NameEL492485
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length195
Libraries
Library NameType
Citrus sinensis flesh cDNA-AFLP Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EL492485 vs. ExPASy Swiss-Prot
Match: UBC2_YEAST (Ubiquitin-conjugating enzyme E2 2 OS=Saccharomyces cerevisiae GN=RAD6 PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 8.928e-12
Identity = 28/53 (52.83%), Postives = 39/53 (73.58%), Query Frame = 1
Query:   22 IMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDIL 180
            I+GP+D+PY  G F + + F  +YP KPP V F +++FHPN+ +NG ICLDIL
Sbjct:   40 IIGPADTPYEDGTFRLLLEFDEEYPNKPPHVKFLSEMFHPNVYANGEICLDIL 92          
BLAST of EL492485 vs. ExPASy Swiss-Prot
Match: UBC2_KLULA (Ubiquitin-conjugating enzyme E2 2 OS=Kluyveromyces lactis GN=UBC2 PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 8.928e-12
Identity = 28/53 (52.83%), Postives = 39/53 (73.58%), Query Frame = 1
Query:   22 IMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDIL 180
            I+GP+D+PY  G F + + F  +YP KPP V F +++FHPN+ +NG ICLDIL
Sbjct:   40 IIGPADTPYEDGTFRLLLEFDEEYPNKPPHVKFLSEMFHPNVYANGEICLDIL 92          
BLAST of EL492485 vs. ExPASy Swiss-Prot
Match: UBC2_ASHGO (Ubiquitin-conjugating enzyme E2 2 OS=Ashbya gossypii GN=UBC2 PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 8.928e-12
Identity = 28/53 (52.83%), Postives = 39/53 (73.58%), Query Frame = 1
Query:   22 IMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDIL 180
            I+GP+D+PY  G F + + F  +YP KPP V F +++FHPN+ +NG ICLDIL
Sbjct:   40 IIGPADTPYEDGTFRLLLEFDEEYPNKPPHVKFLSEMFHPNVYANGEICLDIL 92          
BLAST of EL492485 vs. ExPASy Swiss-Prot
Match: UBE2T_MOUSE (Ubiquitin-conjugating enzyme E2 T OS=Mus musculus GN=Ube2t PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.166e-11
Identity = 29/57 (50.88%), Postives = 42/57 (73.68%), Query Frame = 1
Query:   10 LASTIMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDIL 180
            L + I+G +++PY  GVF + +  P  YPF+PP+V F T ++HPNI+S+G ICLDIL
Sbjct:   34 LRAQILGGANTPYEKGVFTLEVIIPERYPFEPPQVRFLTPIYHPNIDSSGRICLDIL 90          
BLAST of EL492485 vs. ExPASy Swiss-Prot
Match: UBE2N_RAT (Ubiquitin-conjugating enzyme E2 N OS=Rattus norvegicus GN=Ube2n PE=1 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.166e-11
Identity = 28/53 (52.83%), Postives = 36/53 (67.92%), Query Frame = 1
Query:   22 IMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDIL 180
            I GP DSP+ GG F + +  P +YP   PKV F TK++HPN++  G ICLDIL
Sbjct:   39 IAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDIL 91          
BLAST of EL492485 vs. ExPASy Swiss-Prot
Match: UBE2N_PONAB (Ubiquitin-conjugating enzyme E2 N OS=Pongo abelii GN=UBE2N PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.166e-11
Identity = 28/53 (52.83%), Postives = 36/53 (67.92%), Query Frame = 1
Query:   22 IMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDIL 180
            I GP DSP+ GG F + +  P +YP   PKV F TK++HPN++  G ICLDIL
Sbjct:   39 IAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDIL 91          
BLAST of EL492485 vs. ExPASy Swiss-Prot
Match: UBE2N_MOUSE (Ubiquitin-conjugating enzyme E2 N OS=Mus musculus GN=Ube2n PE=1 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.166e-11
Identity = 28/53 (52.83%), Postives = 36/53 (67.92%), Query Frame = 1
Query:   22 IMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDIL 180
            I GP DSP+ GG F + +  P +YP   PKV F TK++HPN++  G ICLDIL
Sbjct:   39 IAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDIL 91          
BLAST of EL492485 vs. ExPASy Swiss-Prot
Match: UBE2N_HUMAN (Ubiquitin-conjugating enzyme E2 N OS=Homo sapiens GN=UBE2N PE=1 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.166e-11
Identity = 28/53 (52.83%), Postives = 36/53 (67.92%), Query Frame = 1
Query:   22 IMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDIL 180
            I GP DSP+ GG F + +  P +YP   PKV F TK++HPN++  G ICLDIL
Sbjct:   39 IAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDIL 91          
BLAST of EL492485 vs. ExPASy Swiss-Prot
Match: UBE2N_BOVIN (Ubiquitin-conjugating enzyme E2 N OS=Bos taurus GN=UBE2N PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.166e-11
Identity = 28/53 (52.83%), Postives = 36/53 (67.92%), Query Frame = 1
Query:   22 IMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNINSNGSICLDIL 180
            I GP DSP+ GG F + +  P +YP   PKV F TK++HPN++  G ICLDIL
Sbjct:   39 IAGPQDSPFEGGTFKLELFLPEEYPMAAPKVRFMTKIYHPNVDKLGRICLDIL 91          
BLAST of EL492485 vs. ExPASy Swiss-Prot
Match: UBC21_ARATH (Probable ubiquitin carrier protein E2 21 OS=Arabidopsis thaliana GN=UBC21 PE=1 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.166e-11
Identity = 30/54 (55.56%), Postives = 38/54 (70.37%), Query Frame = 1
Query:   22 IMGPSDSPYAGGVFLVTIHFPPDYPFKPPKVAFRTKVFHPNIN-SNGSICLDIL 180
            I GPS++PY GGVF +    P  YP +PP+V F TK+FHPN++   G ICLDIL
Sbjct:   41 IKGPSETPYEGGVFQLAFSVPEPYPLQPPQVRFLTKIFHPNVHFKTGEICLDIL 94          
The following BLAST results are available for this feature:
BLAST of EL492485 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 121
Match NameE-valueIdentityDescription
UBC2_YEAST8.928e-1252.83Ubiquitin-conjugating enzyme E2 2 OS=Saccharomyces... [more]
UBC2_KLULA8.928e-1252.83Ubiquitin-conjugating enzyme E2 2 OS=Kluyveromyces... [more]
UBC2_ASHGO8.928e-1252.83Ubiquitin-conjugating enzyme E2 2 OS=Ashbya gossyp... [more]
UBE2T_MOUSE1.166e-1150.88Ubiquitin-conjugating enzyme E2 T OS=Mus musculus ... [more]
UBE2N_RAT1.166e-1152.83Ubiquitin-conjugating enzyme E2 N OS=Rattus norveg... [more]
UBE2N_PONAB1.166e-1152.83Ubiquitin-conjugating enzyme E2 N OS=Pongo abelii ... [more]
UBE2N_MOUSE1.166e-1152.83Ubiquitin-conjugating enzyme E2 N OS=Mus musculus ... [more]
UBE2N_HUMAN1.166e-1152.83Ubiquitin-conjugating enzyme E2 N OS=Homo sapiens ... [more]
UBE2N_BOVIN1.166e-1152.83Ubiquitin-conjugating enzyme E2 N OS=Bos taurus GN... [more]
UBC21_ARATH1.166e-1155.56Probable ubiquitin carrier protein E2 21 OS=Arabid... [more]

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Properties
Property NameValue
Genbank descriptionC1180 Citrus sinensis flesh cDNA-AFLP Library Citrus sinensis cDNA clone ClC1180, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EL492485 ID=EL492485; Name=EL492485; organism=Citrus sinensis; type=EST; length=195bp
CATGTTCCACTGGCAAGCACAATTATGGGCCCTTCTGATAGCCCATATGC
AGGAGGTGTATTTTTGGTTACCATTCATTTTCCTCCTGATTATCCNTTCA
AGCCCCCAAAGGTTGCTTTTAGGACCAAGGTCTTCCACCCGAACATCAAC
AGCAATGGAAGCATCTGTCTTGATATCCTTACTNCAGGACTCATC
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