EY665446 | Citrus Genome Database

Overview


Name	EY665446
Unique Name	EY665446
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	843

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 14335_ORYSJ (14-3-3-like protein GF14-E OS=Oryza sativa subsp. japonica GN=GF14E PE=2 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 5.025e-30
Identity = 54/121 (44.63%), Postives = 75/121 (61.98%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPH---CDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISSI+Q EES G + R  L   + E  G         CDGIL L++SHL+PS+++ ES   YL M GD++    +F+T   + D A N++  YK+  +IA+ +L PT PIRLGLALN S
Sbjct:   68 RIISSIEQKEESRGNEDRCTL---IKEYRGKIETELSKICDGILKLLDSHLVPSSTAPESKVFYLKMKGDYYRYLAEFKTGAERKDAAENTMVAYKAAQDIALAELPPTHPIRLGLALNFS 185

HSP 2 Score: 60.8474 bits (146), Expect = 5.025e-30
Identity = 31/65 (47.69%), Postives = 42/65 (64.62%), Query Frame = 3

Query:    3 PNESSPLWDVYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            P E S   +VYMAK+A+Q   Y +M EFM   AK+  +++LT ++R  LS AY NVIG   ASW+
Sbjct:    4 PAELSREENVYMAKLAEQAERYEEMVEFMEKVAKTVDSEELTVEERNLLSVAYKNVIGARRASWR 68

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433_LILLO (14-3-3-like protein OS=Lilium longiflorum PE=2 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 6.545e-30
Identity = 55/121 (45.45%), Postives = 78/121 (64.46%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFL---N*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISSI+Q EES G +  V L     G +E + +     CDGIL L++SHL+PS+++ ES   YL M GD+H    +F++   + + A ++L  YKS  +IA+ +LAPT PIRLGLALN S
Sbjct:   67 RIISSIEQKEESRGNEDHVALIKDYRGKIEAELS---KICDGILKLLDSHLVPSSTAPESKVFYLKMKGDYHRYLAEFKSGAERKEAAESTLLAYKSAQDIALAELAPTHPIRLGLALNFS 184

HSP 2 Score: 59.6918 bits (143), Expect = 6.545e-30
Identity = 30/65 (46.15%), Postives = 41/65 (63.08%), Query Frame = 3

Query:    3 PNESSPLWDVYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            P E S   +VYMAK+A+Q   Y +M EFM   A++   ++LT ++R  LS AY NVIG   ASW+
Sbjct:    3 PAEPSREENVYMAKLAEQAERYEEMVEFMEKVARTVDTEELTVEERNLLSVAYKNVIGARRASWR 67

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 14336_ORYSJ (14-3-3-like protein GF14-F OS=Oryza sativa subsp. japonica GN=GF14F PE=1 SV=2)

HSP 1 Score: 88.1965 bits (217), Expect = 2.449e-29
Identity = 53/126 (42.06%), Postives = 74/126 (58.73%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFD--------YRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISSI+Q EES G +        YR  +   + ++        CDGIL L++SHL+PSA++ ES   YL M GD+H    +F++   + + A N+L  YKS  +IA+  L  T PIRLGLALN S
Sbjct:   67 RIISSIEQKEESRGNEAYVASIKEYRSRIETELSKI--------CDGILKLLDSHLVPSATAAESKVFYLKMKGDYHRYLAEFKSGAERKEAAENTLVAYKSAQDIALADLPTTHPIRLGLALNFS 184

HSP 2 Score: 62.3882 bits (150), Expect = 2.449e-29
Identity = 32/65 (49.23%), Postives = 42/65 (64.62%), Query Frame = 3

Query:    3 PNESSPLWDVYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            P E+S   +VYMAK+A+Q   Y +M EFM   AK+T   +LT ++R  LS AY NVIG   ASW+
Sbjct:    3 PAEASREENVYMAKLAEQAERYEEMVEFMEKVAKTTDVGELTVEERNLLSVAYKNVIGARRASWR 67

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 14331_SOLTU (14-3-3-like protein OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 3.189e-29
Identity = 60/127 (47.24%), Postives = 79/127 (62.20%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFD--------YRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFET-APFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISSI+Q EES G +        YRV +   + ++        CDGILSL+ESHL+PSAS+ ES   YL M GD+H    +F+T A  + + A N+L  YKS  +IA+ +L PT PIRLGLALN S
Sbjct:   63 RIISSIEQKEESRGNEDHVSSIKEYRVKIEAELSKI--------CDGILSLLESHLVPSASTAESKVFYLKMKGDYHRYLAEFKTGARERKEAAENTLLAYKSAQDIALAELTPTHPIRLGLALNFS 181

HSP 2 Score: 53.9138 bits (128), Expect = 3.189e-29
Identity = 28/57 (49.12%), Postives = 37/57 (64.91%), Query Frame = 3

Query:   27 DVYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +VYMAK+A+Q   Y +M EFM   AK    ++LT ++R  LS AY NVIG   ASW+
Sbjct:    8 NVYMAKLAEQAERYEEMVEFMEKVAK-VDVEELTVEERNLLSVAYKNVIGARRASWR 63

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 14333_ARATH (14-3-3-like protein GF14 psi OS=Arabidopsis thaliana GN=GRF3 PE=1 SV=2)

HSP 1 Score: 92.4337 bits (228), Expect = 4.153e-29
Identity = 55/121 (45.45%), Postives = 76/121 (62.81%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFL---N*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISSI+Q EES G +  V +     G +E + +     CDGIL+++E+HL+PSAS  ES   YL M GD+H    +F+    + + A ++L  YKS  +IA  +LAPT PIRLGLALN S
Sbjct:   63 RIISSIEQKEESKGNEDHVAIIKDYRGKIESELS---KICDGILNVLEAHLIPSASPAESKVFYLKMKGDYHRYLAEFKAGAERKEAAESTLVAYKSASDIATAELAPTHPIRLGLALNFS 180

HSP 2 Score: 57.3806 bits (137), Expect = 4.153e-29
Identity = 27/57 (47.37%), Postives = 38/57 (66.67%), Query Frame = 3

Query:   27 DVYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +VYMAK+A+Q   Y +M EFM   AK+   ++L+ ++R  LS AY NVIG   ASW+
Sbjct:    7 NVYMAKLAEQAERYEEMVEFMEKVAKTVDVEELSVEERNLLSVAYKNVIGARRASWR 63

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 14333_ORYSJ (14-3-3-like protein GF14-C OS=Oryza sativa subsp. japonica GN=GF14C PE=1 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 7.041e-29
Identity = 52/121 (42.98%), Postives = 74/121 (61.16%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPH---CDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +I+SSI+Q EE  G +  V L   + E  G         CDGIL L++SHL+PS+++ ES   YL M GD+H    +F+T   + + A +++  YK+  +IA+  LAPT PIRLGLALN S
Sbjct:   62 RIVSSIEQKEEGRGNEEHVTL---IKEYRGKIEAELSKICDGILKLLDSHLVPSSTAAESKVFYLKMKGDYHRYLAEFKTGAERKEAAESTMVAYKAAQDIALADLAPTHPIRLGLALNFS 179

HSP 2 Score: 57.7658 bits (138), Expect = 7.041e-29
Identity = 27/57 (47.37%), Postives = 38/57 (66.67%), Query Frame = 3

Query:   27 DVYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +VYMAK+A+Q   Y +M E+M   AK+   ++LT ++R  LS AY NVIG   ASW+
Sbjct:    6 NVYMAKLAEQAERYEEMVEYMEKVAKTVDVEELTVEERNLLSVAYKNVIGARRASWR 62

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 14332_ARATH (14-3-3-like protein GF14 omega OS=Arabidopsis thaliana GN=GRF2 PE=1 SV=2)

HSP 1 Score: 92.0485 bits (227), Expect = 9.165e-29
Identity = 56/123 (45.53%), Postives = 75/123 (60.98%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFD-----YRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISSI+Q EES G D      R + +    E+ G      CDGIL L++S L+P+A+S +S   YL M GD+H    +F+T   + D A ++L  YKS  +IA  +LAPT PIRLGLALN S
Sbjct:   64 RIISSIEQKEESRGNDDHVTAIREYRSKIETELSGI-----CDGILKLLDSRLIPAAASGDSKVFYLKMKGDYHRYLAEFKTGQERKDAAEHTLAAYKSAQDIANAELAPTHPIRLGLALNFS 181

HSP 2 Score: 56.6102 bits (135), Expect = 9.165e-29
Identity = 27/56 (48.21%), Postives = 36/56 (64.29%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            VYMAK+A+Q   Y +M EFM   + +   D+LT ++R  LS AY NVIG   ASW+
Sbjct:    9 VYMAKLAEQAERYEEMVEFMEKVSAAVDGDELTVEERNLLSVAYKNVIGARRASWR 64

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 14331_ARATH (14-3-3-like protein GF14 chi OS=Arabidopsis thaliana GN=GRF1 PE=1 SV=3)

HSP 1 Score: 87.4261 bits (215), Expect = 2.631e-28
Identity = 52/118 (44.07%), Postives = 71/118 (60.17%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISSI+Q EES G D  V L               CDGIL L+++ L+P+A+S +S   YL M GD+H    +F++   + D A ++L  YK+  +IA  +LAPT PIRLGLALN S
Sbjct:   69 RIISSIEQKEESRGNDDHVSLIRDYRSKIETELSDICDGILKLLDTILVPAAASGDSKVFYLKMKGDYHRYLAEFKSGQERKDAAEHTLTAYKAAQDIANSELAPTHPIRLGLALNFS 186

HSP 2 Score: 59.6918 bits (143), Expect = 2.631e-28
Identity = 29/56 (51.79%), Postives = 37/56 (66.07%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            VYMAK+A+Q   Y +M EFM   AK+   D+LT ++R  LS AY NVIG   ASW+
Sbjct:   14 VYMAKLAEQAERYEEMVEFMEKVAKAVDKDELTVEERNLLSVAYKNVIGARRASWR 69

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433A_HORVU (14-3-3-like protein A OS=Hordeum vulgare PE=2 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 4.462e-28
Identity = 53/126 (42.06%), Postives = 73/126 (57.94%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFD--------YRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISSI+Q EES G +        YR  +   + ++        CDGIL L++SHL+PSA++ ES   YL M GD+H    +F+    + + A N+L  YKS  +IA+  L  T PIRLGLALN S
Sbjct:   67 RIISSIEQKEESRGNEAYVASIKEYRTRIETELSKI--------CDGILKLLDSHLVPSATAAESKVFYLKMKGDYHRYLAEFKAGAERKEAAENTLVAYKSAQDIALADLPTTHPIRLGLALNFS 184

HSP 2 Score: 57.7658 bits (138), Expect = 4.462e-28
Identity = 28/57 (49.12%), Postives = 37/57 (64.91%), Query Frame = 3

Query:   27 DVYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +VYMAK+A+Q   Y +M EFM   AK+    +LT ++R  LS AY NVIG   ASW+
Sbjct:   11 NVYMAKLAEQAERYEEMVEFMEKVAKTADVGELTVEERNLLSVAYKNVIGARRASWR 67

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433C_TOBAC (14-3-3-like protein C OS=Nicotiana tabacum PE=1 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 9.845e-28
Identity = 52/118 (44.07%), Postives = 71/118 (60.17%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISSI+Q EES G +  V           N     CDGIL L+++ L+PSA+S +S   YL M GD+H    +F+T   + + A ++L  YK+  +IA  +LAPT PIRLGLALN S
Sbjct:   67 RIISSIEQKEESRGNEEHVNSIREYRSKIENELSKICDGILKLLDAKLIPSAASGDSKVFYLKMKGDYHRYLAEFKTGAERKEAAESTLTAYKAAQDIATTELAPTHPIRLGLALNFS 184

HSP 2 Score: 56.6102 bits (135), Expect = 9.845e-28
Identity = 27/57 (47.37%), Postives = 38/57 (66.67%), Query Frame = 3

Query:   27 DVYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +VYMAK+A+Q   Y +M EFM   + S  +++LT ++R  LS AY NVIG   ASW+
Sbjct:   11 NVYMAKLAEQAERYEEMVEFMEKVSNSLGSEELTVEERNLLSVAYKNVIGARRASWR 67

The following BLAST results are available for this feature:

BLAST of EY665446 vs. ExPASy Swiss-Prot

BLAST of EY665446 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 130


Match Name	E-value	Identity	Description
14335_ORYSJ	5.025e-30	44.63	14-3-3-like protein GF14-E OS=Oryza sativa subsp. ...	[more]
1433_LILLO	6.545e-30	45.45	14-3-3-like protein OS=Lilium longiflorum PE=2 SV=...	[more]
14336_ORYSJ	2.449e-29	42.06	14-3-3-like protein GF14-F OS=Oryza sativa subsp. ...	[more]
14331_SOLTU	3.189e-29	47.24	14-3-3-like protein OS=Solanum tuberosum PE=2 SV=1	[more]
14333_ARATH	4.153e-29	45.45	14-3-3-like protein GF14 psi OS=Arabidopsis thalia...	[more]
14333_ORYSJ	7.041e-29	42.98	14-3-3-like protein GF14-C OS=Oryza sativa subsp. ...	[more]
14332_ARATH	9.165e-29	45.53	14-3-3-like protein GF14 omega OS=Arabidopsis thal...	[more]
14331_ARATH	2.631e-28	44.07	14-3-3-like protein GF14 chi OS=Arabidopsis thalia...	[more]
1433A_HORVU	4.462e-28	42.06	14-3-3-like protein A OS=Hordeum vulgare PE=2 SV=1	[more]
1433C_TOBAC	9.845e-28	44.07	14-3-3-like protein C OS=Nicotiana tabacum PE=1 SV...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-102-002-B09-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY665446 ID=EY665446; Name=EY665446; organism=Citrus sinensis; type=EST; length=843bp
CGCCTAATGAGTCTTCTCCCTTATGGGATGTCTACATGGCCAAGATGGCC
GACCAGGTTGTCCTTTATTGGGATATGGCTGAGTTCATGCACCATTTCGC
AAAATCAACCCATGCTGATGACCTAACCTGGGATGACCGGAAACATCTCT
CATTTGCTTACAACAATGTGATTGGGGTGGGAATGGCTTCATGGAAAGAT
CATCTCTTCTATTGATCAGAATGAGGAGAGCATGGGATTCGATTACCGTG
TCTTCTTGAATTAAGGAGTACTAGAGATGGATGGAAATTGACCTCTCCCT
CATTGTGATGGGATTCTTAGCCTCATTGAATCTCATCTCCTTCCCTCTGC
CTCTTCGGTTGAGTCTAATGCGCTCTACCTTCCTATGGATGGTGATCACC
ACTTGGATCCGTTTCAGTTTGAGACTGCACCTTTTAAGACCGATACTGCG
CCGAACTCTTTGCCGGACTACAAGTCAACTCACGAAATCGCTATACCTCA
ATTGGCCCCTACTCCCCCCATACGGCTGGGACTGGCACTCAATGTCTCCT
GTGTTCGACTATGACATCCGTTGATCCCCCGATTTTGGATGGCATCCTTG
CAAAGCAAGCTTTTGATTGACCGCCTTTTCTGAGCTTGATAACCTTGGGT
GAAAGATCATGACTAGGAATTTTCTTTGGTATATAACTTTCTCTCGAAAA
AAATCTAACCCTTTAGGACATCTTGTAATCACTGGTGATGGTGGGAAATA
AATTACCGGAAAACAAAAACTAATCCTGAATTCAGGGGAAAACCGGACAA
CTGCTTGAAGTATTGATTTCCCGGTCCTACGATTTGTTACCCG