EY665446 | Citrus Genome Database

Overview


Name	EY665446
Unique Name	EY665446
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	843

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433Z_SHEEP (14-3-3 protein zeta/delta OS=Ovis aries GN=YWHAZ PE=1 SV=1)

HSP 1 Score: 61.2326 bits (147), Expect = 8.408e-14
Identity = 40/124 (32.26%), Postives = 61/124 (49.19%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +++SSI+Q  E       M  +YR  +   + ++        C+ +LSL+E  L+P+ S  ES   YL M GD++    +      K      S   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   60 RVVSSIEQKTEGAEKKQQMAREYREKIETELRDI--------CNDVLSLLEKFLIPNRSQPESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFS 175

HSP 2 Score: 36.965 bits (84), Expect = 8.408e-14
Identity = 21/56 (37.50%), Postives = 31/56 (55.36%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +     +L+ ++R  LS AY NV+G   +SW+
Sbjct:    7 VQKAKLAEQAERYDDMAACMKSVTEQ--GAELSNEERNLLSVAYKNVVGARRSSWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433Z_DROME (14-3-3 protein zeta OS=Drosophila melanogaster GN=14-3-3zeta PE=1 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 1.092e-13
Identity = 39/124 (31.45%), Postives = 64/124 (51.61%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E+      +  +YR  +   + E+        C  +L L++ +L+P AS+ ES   YL M GD++    +  T   +     +S   Y+   +I+  ++ PT PIRLGLALN S
Sbjct:   63 RVISSIEQKTEASARKQQLAREYRERVEKELREI--------CYEVLGLLDKYLIPKASNPESKVFYLKMKGDYYRYLAEVATGDARNTVVDDSQTAYQDAFDISKGKMQPTHPIRLGLALNFS 178

HSP 2 Score: 37.3502 bits (85), Expect = 1.092e-13
Identity = 21/56 (37.50%), Postives = 33/56 (58.93%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA+ M    ++    +L+ ++R  LS AY NV+G   +SW+
Sbjct:   10 VQKAKLAEQSERYDDMAQAMKSVTET--GVELSNEERNLLSVAYKNVVGARRSSWR 63

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 143BA_XENLA (14-3-3 protein beta/alpha-A OS=Xenopus laevis GN=ywhab-A PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.093e-13
Identity = 40/124 (32.26%), Postives = 64/124 (51.61%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E       M  +YR  +   + ++        C  +L L++  L+P+A+  ES   YL M GD++    +  +   K +T  +S   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   60 RVISSIEQKTEGNDKRQQMAREYREKVETELQDI--------CKDVLDLLDRFLVPNATPPESKVFYLKMKGDYYRYLSEVASGDSKQETVASSQQAYQEAFEISKSEMQPTHPIRLGLALNFS 175

HSP 2 Score: 35.8094 bits (81), Expect = 1.093e-13
Identity = 21/56 (37.50%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+++Q   Y DMA  M   A +    +L+ ++R  LS AY NV+G   +SW+
Sbjct:    7 VQKAKLSEQAERYDDMAASMK--AVTELGAELSNEERNLLSVAYKNVVGARRSSWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433B_XENTR (14-3-3 protein beta/alpha OS=Xenopus tropicalis GN=ywhab PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.093e-13
Identity = 40/124 (32.26%), Postives = 64/124 (51.61%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E       M  +YR  +   + ++        C  +L L++ +L+P+A+  ES   YL M GD++    +  +   K +T   S   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   60 RVISSIEQKTEGNDKRQQMAREYREKVETELQDI--------CKDVLGLLDKYLVPNATPPESKVFYLKMKGDYYRYLSEVASGDSKQETVTCSQQAYQEAFEISKSEMQPTHPIRLGLALNFS 175

HSP 2 Score: 35.8094 bits (81), Expect = 1.093e-13
Identity = 21/56 (37.50%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+++Q   Y DMA  M   A +    +L+ ++R  LS AY NV+G   +SW+
Sbjct:    7 VQKAKLSEQAERYDDMAASMK--AVTELGAELSNEERNLLSVAYKNVVGARRSSWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433Z_XENLA (14-3-3 protein zeta OS=Xenopus laevis GN=ywhaz PE=2 SV=2)

HSP 1 Score: 59.6918 bits (143), Expect = 1.419e-13
Identity = 38/124 (30.65%), Postives = 63/124 (50.81%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +++SSI+Q  E       M  +YR  +   + E+        C+ +L+L++  L+ +A+  ES   YL M GD++    +      KT+    S   Y+   +I+  ++ PT PIRLGLALN S
Sbjct:   60 RVVSSIEQKTEGAEKKQEMSREYREKIEAELREI--------CNDVLNLLDKFLIANATQPESKVFYLKMKGDYYRYLAEVAAGNAKTEIVGQSQKAYQDAFDISKTEMQPTHPIRLGLALNFS 175

HSP 2 Score: 37.7354 bits (86), Expect = 1.419e-13
Identity = 21/56 (37.50%), Postives = 31/56 (55.36%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +     +L+ ++R  LS AY NV+G   +SW+
Sbjct:    7 VQKAKLAEQAERYDDMAACMKRVTEE--GGELSNEERNLLSVAYKNVVGARRSSWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433_XENLA (14-3-3-like protein (Fragment) OS=Xenopus laevis PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.847e-13
Identity = 40/124 (32.26%), Postives = 64/124 (51.61%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E       M  +YR  +   + ++        C  +L L++  L+P+A+  ES   YL M GD++    +  +   K +T  +S   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   51 RVISSIEQKTEGNDKRQQMAREYREKVETELQDI--------CKDVLDLLDRFLVPNATPPESKVFYLKMKGDYYRYLSEVASGDSKQETVASSQQAYQEAFEISKSEMQPTHPIRLGLALNFS 166

HSP 2 Score: 35.039 bits (79), Expect = 1.847e-13
Identity = 20/53 (37.74%), Postives = 31/53 (58.49%), Query Frame = 3

Query:   39 AKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            AK+++Q   Y DMA  M   A +    +L+ ++R  LS AY NV+G   +SW+
Sbjct:    1 AKLSEQAERYDDMAASMK--AVTELGAELSNEERNLLSVAYKNVVGARRSSWR 51

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433G_BOVIN (14-3-3 protein gamma OS=Bos taurus GN=YWHAG PE=1 SV=2)

HSP 1 Score: 60.4622 bits (145), Expect = 2.395e-13
Identity = 38/120 (31.67%), Postives = 61/120 (50.83%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASS--VESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q   + G + ++ +     E         C  +LSL++++L+ + S   +ES   YL M GD++    +  T   +     +S   Y   HEI+   + PT PIRLGLALN S
Sbjct:   61 RVISSIEQKTSADGNEKKIEMVRAYREKIEKELEAVCQDVLSLLDNYLIKNCSETQIESKVFYLKMKGDYYRYLAEVATGEKRATVVESSEKAYSEAHEISKEHMQPTHPIRLGLALNYS 180

HSP 2 Score: 36.1946 bits (82), Expect = 2.395e-13
Identity = 20/56 (35.71%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  A++A+Q   Y DMA  M +  +    + L+ ++R  LS AY NV+G   +SW+
Sbjct:    8 VQKARLAEQAERYDDMAAAMKNVTELN--EPLSNEERNLLSVAYKNVVGARRSSWR 61

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 14332_CAEEL (14-3-3-like protein 2 OS=Caenorhabditis elegans GN=ftt-2 PE=1 SV=1)

HSP 1 Score: 60.4622 bits (145), Expect = 3.109e-13
Identity = 38/124 (30.65%), Postives = 61/124 (49.19%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E       M  +YR  +   + ++        C  +L+L++  L+P A + ES   YL M GD++    +  +   +      S   Y+   +IA  ++ PT PIRLGLALN S
Sbjct:   62 RVISSIEQKTEGSEKKQQMAKEYREKVEKELRDI--------CQDVLNLLDKFLIPKAGAAESKVFYLKMKGDYYRYLAEVASGDDRNSVVEKSQQSYQEAFDIAKDKMQPTHPIRLGLALNFS 177

HSP 2 Score: 35.8094 bits (81), Expect = 3.109e-13
Identity = 21/56 (37.50%), Postives = 31/56 (55.36%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +     +L+ ++R  LS AY NV+G   +SW+
Sbjct:    9 VNRAKLAEQAERYDDMAASMKKVTEL--GAELSNEERNLLSVAYKNVVGARRSSWR 62

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433G_RAT (14-3-3 protein gamma OS=Rattus norvegicus GN=Ywhag PE=1 SV=2)

HSP 1 Score: 59.3066 bits (142), Expect = 5.248e-13
Identity = 38/120 (31.67%), Postives = 60/120 (50.00%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSV--ESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q   + G + ++ +     E         C  +LSL++++L+ + S    ES   YL M GD++    +  T   +     +S   Y   HEI+   + PT PIRLGLALN S
Sbjct:   61 RVISSIEQKTSADGNEKKIEMVRAYREKIEKELEAVCQDVLSLLDNYLIKNCSETQYESKVFYLKMKGDYYRYLAEVATGEKRATVVESSEKAYSEAHEISKEHMQPTHPIRLGLALNYS 180

HSP 2 Score: 36.1946 bits (82), Expect = 5.248e-13
Identity = 20/56 (35.71%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  A++A+Q   Y DMA  M +  +    + L+ ++R  LS AY NV+G   +SW+
Sbjct:    8 VQKARLAEQAERYDDMAAAMKNVTELN--EPLSNEERNLLSVAYKNVVGARRSSWR 61

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433G_PONAB (14-3-3 protein gamma OS=Pongo abelii GN=YWHAG PE=2 SV=3)

HSP 1 Score: 59.3066 bits (142), Expect = 5.248e-13
Identity = 38/120 (31.67%), Postives = 60/120 (50.00%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSV--ESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q   + G + ++ +     E         C  +LSL++++L+ + S    ES   YL M GD++    +  T   +     +S   Y   HEI+   + PT PIRLGLALN S
Sbjct:   61 RVISSIEQKTSADGNEKKIEMVRAYREKIEKELEAVCQDVLSLLDNYLIKNCSETQYESKVFYLKMKGDYYRYLAEVATGEKRATVVESSEKAYSEAHEISKEHMQPTHPIRLGLALNYS 180

HSP 2 Score: 36.1946 bits (82), Expect = 5.248e-13
Identity = 20/56 (35.71%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  A++A+Q   Y DMA  M +  +    + L+ ++R  LS AY NV+G   +SW+
Sbjct:    8 VQKARLAEQAERYDDMAAAMKNVTELN--EPLSNEERNLLSVAYKNVVGARRSSWR 61

The following BLAST results are available for this feature:

BLAST of EY665446 vs. ExPASy Swiss-Prot

BLAST of EY665446 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 130


Match Name	E-value	Identity	Description
1433Z_SHEEP	8.408e-14	32.26	14-3-3 protein zeta/delta OS=Ovis aries GN=YWHAZ P...	[more]
1433Z_DROME	1.092e-13	31.45	14-3-3 protein zeta OS=Drosophila melanogaster GN=...	[more]
143BA_XENLA	1.093e-13	32.26	14-3-3 protein beta/alpha-A OS=Xenopus laevis GN=y...	[more]
1433B_XENTR	1.093e-13	32.26	14-3-3 protein beta/alpha OS=Xenopus tropicalis GN...	[more]
1433Z_XENLA	1.419e-13	30.65	14-3-3 protein zeta OS=Xenopus laevis GN=ywhaz PE=...	[more]
1433_XENLA	1.847e-13	32.26	14-3-3-like protein (Fragment) OS=Xenopus laevis P...	[more]
1433G_BOVIN	2.395e-13	31.67	14-3-3 protein gamma OS=Bos taurus GN=YWHAG PE=1 S...	[more]
14332_CAEEL	3.109e-13	30.65	14-3-3-like protein 2 OS=Caenorhabditis elegans GN...	[more]
1433G_RAT	5.248e-13	31.67	14-3-3 protein gamma OS=Rattus norvegicus GN=Ywhag...	[more]
1433G_PONAB	5.248e-13	31.67	14-3-3 protein gamma OS=Pongo abelii GN=YWHAG PE=2...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-102-002-B09-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY665446 ID=EY665446; Name=EY665446; organism=Citrus sinensis; type=EST; length=843bp
CGCCTAATGAGTCTTCTCCCTTATGGGATGTCTACATGGCCAAGATGGCC
GACCAGGTTGTCCTTTATTGGGATATGGCTGAGTTCATGCACCATTTCGC
AAAATCAACCCATGCTGATGACCTAACCTGGGATGACCGGAAACATCTCT
CATTTGCTTACAACAATGTGATTGGGGTGGGAATGGCTTCATGGAAAGAT
CATCTCTTCTATTGATCAGAATGAGGAGAGCATGGGATTCGATTACCGTG
TCTTCTTGAATTAAGGAGTACTAGAGATGGATGGAAATTGACCTCTCCCT
CATTGTGATGGGATTCTTAGCCTCATTGAATCTCATCTCCTTCCCTCTGC
CTCTTCGGTTGAGTCTAATGCGCTCTACCTTCCTATGGATGGTGATCACC
ACTTGGATCCGTTTCAGTTTGAGACTGCACCTTTTAAGACCGATACTGCG
CCGAACTCTTTGCCGGACTACAAGTCAACTCACGAAATCGCTATACCTCA
ATTGGCCCCTACTCCCCCCATACGGCTGGGACTGGCACTCAATGTCTCCT
GTGTTCGACTATGACATCCGTTGATCCCCCGATTTTGGATGGCATCCTTG
CAAAGCAAGCTTTTGATTGACCGCCTTTTCTGAGCTTGATAACCTTGGGT
GAAAGATCATGACTAGGAATTTTCTTTGGTATATAACTTTCTCTCGAAAA
AAATCTAACCCTTTAGGACATCTTGTAATCACTGGTGATGGTGGGAAATA
AATTACCGGAAAACAAAAACTAATCCTGAATTCAGGGGAAAACCGGACAA
CTGCTTGAAGTATTGATTTCCCGGTCCTACGATTTGTTACCCG