EY665446 | Citrus Genome Database

Overview


Name	EY665446
Unique Name	EY665446
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	843

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433C_SOYBN (14-3-3-like protein C OS=Glycine max GN=GF14C PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 2.634e-16
Identity = 41/126 (32.54%), Postives = 69/126 (54.76%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFD--------YRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +I+SSI+Q EE+ G D        YR+ +   +  +        C  I+++I+ +L+PS+SS E +  +  M GD++    +F++   + + A +S+  Y+     A  +LA T PIRLGLALN S
Sbjct:   64 RILSSIEQKEEAKGNDVSVKRIKEYRLKVESELSNI--------CSDIMTVIDEYLIPSSSSGEPSVFFYKMKGDYYRYLAEFKSGDERKEAADHSMKAYQLASTTAEAELASTHPIRLGLALNFS 181

HSP 2 Score: 42.743 bits (99), Expect = 2.634e-16
Identity = 23/56 (41.07%), Postives = 33/56 (58.93%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            VY+AK+A+Q   Y +M E M + A      +LT ++R  LS  Y NV+G   ASW+
Sbjct:   11 VYVAKLAEQAERYEEMVEAMKNVANLNV--ELTVEERNLLSVGYKNVVGARRASWR 64

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433B_PONAB (14-3-3 protein beta/alpha OS=Pongo abelii GN=YWHAB PE=3 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 2.638e-16
Identity = 42/124 (33.87%), Postives = 65/124 (52.42%), Query Frame = 1

Query:  196 KIISSIDQNEE------SMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E       MG +YR  +   + ++        C+ +L L++ +L+P+A+  ES   YL M GD+     +  +   K  T  NS   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   62 RVISSIEQKTERNEKKQQMGKEYREKIEAELQDI--------CNDVLELLDKYLIPNATQPESKVFYLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKKEMQPTHPIRLGLALNFS 177

HSP 2 Score: 39.6614 bits (91), Expect = 2.638e-16
Identity = 22/56 (39.29%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +  H  +L+ ++R  LS AY NV+G   +SW+
Sbjct:    9 VQKAKLAEQAERYDDMAAAMKAVTEQGH--ELSNEERNLLSVAYKNVVGARRSSWR 62

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433B_MACFA (14-3-3 protein beta/alpha OS=Macaca fascicularis GN=YWHAB PE=2 SV=3)

HSP 1 Score: 67.0106 bits (162), Expect = 2.638e-16
Identity = 42/124 (33.87%), Postives = 65/124 (52.42%), Query Frame = 1

Query:  196 KIISSIDQNEE------SMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E       MG +YR  +   + ++        C+ +L L++ +L+P+A+  ES   YL M GD+     +  +   K  T  NS   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   62 RVISSIEQKTERNEKKQQMGKEYREKIEAELQDI--------CNDVLELLDKYLIPNATQPESKVFYLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKKEMQPTHPIRLGLALNFS 177

HSP 2 Score: 39.6614 bits (91), Expect = 2.638e-16
Identity = 22/56 (39.29%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +  H  +L+ ++R  LS AY NV+G   +SW+
Sbjct:    9 VQKAKLAEQAERYDDMAAAMKAVTEQGH--ELSNEERNLLSVAYKNVVGARRSSWR 62

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433B_HUMAN (14-3-3 protein beta/alpha OS=Homo sapiens GN=YWHAB PE=1 SV=3)

HSP 1 Score: 67.0106 bits (162), Expect = 2.638e-16
Identity = 42/124 (33.87%), Postives = 65/124 (52.42%), Query Frame = 1

Query:  196 KIISSIDQNEE------SMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E       MG +YR  +   + ++        C+ +L L++ +L+P+A+  ES   YL M GD+     +  +   K  T  NS   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   62 RVISSIEQKTERNEKKQQMGKEYREKIEAELQDI--------CNDVLELLDKYLIPNATQPESKVFYLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKKEMQPTHPIRLGLALNFS 177

HSP 2 Score: 39.6614 bits (91), Expect = 2.638e-16
Identity = 22/56 (39.29%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +  H  +L+ ++R  LS AY NV+G   +SW+
Sbjct:    9 VQKAKLAEQAERYDDMAAAMKAVTEQGH--ELSNEERNLLSVAYKNVVGARRSSWR 62

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433B_BOVIN (14-3-3 protein beta/alpha OS=Bos taurus GN=YWHAB PE=1 SV=2)

HSP 1 Score: 67.0106 bits (162), Expect = 2.638e-16
Identity = 42/124 (33.87%), Postives = 65/124 (52.42%), Query Frame = 1

Query:  196 KIISSIDQNEE------SMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E       MG +YR  +   + ++        C+ +L L++ +L+P+A+  ES   YL M GD+     +  +   K  T  NS   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   62 RVISSIEQKTERNEKKQQMGKEYREKIEAELQDI--------CNDVLQLLDKYLIPNATQPESKVFYLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKKEMQPTHPIRLGLALNFS 177

HSP 2 Score: 39.6614 bits (91), Expect = 2.638e-16
Identity = 22/56 (39.29%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +  H  +L+ ++R  LS AY NV+G   +SW+
Sbjct:    9 VQKAKLAEQAERYDDMAAAMKAVTEQGH--ELSNEERNLLSVAYKNVVGARRSSWR 62

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433_FUCVE (14-3-3-like protein OS=Fucus vesiculosus PE=2 SV=1)

HSP 1 Score: 63.1586 bits (152), Expect = 9.781e-16
Identity = 33/82 (40.24%), Postives = 49/82 (59.76%), Query Frame = 1

Query:  304 CDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            C  IL +IE+ L+P+++S E    Y  M GD+H    +F++A  +  +A ++L  Y+   + A   L PT PIRLGLALN S
Sbjct:   94 CADILKIIEAELIPNSTSEEGKVFYYKMKGDYHRYLAEFQSADERKTSASDALDAYQLASDHANQDLPPTHPIRLGLALNFS 175

HSP 2 Score: 41.5874 bits (96), Expect = 9.781e-16
Identity = 23/56 (41.07%), Postives = 34/56 (60.71%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            VYMAK+A+Q   + +M + M   A+     +L+ ++R  LS AY NVIG   ASW+
Sbjct:    8 VYMAKLAEQAERFDEMVDHMKAVAQQPK--ELSVEERNLLSVAYKNVIGSRRASWR 61

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433Z_BOMMO (14-3-3 protein zeta OS=Bombyx mori GN=14-3-3zeta PE=2 SV=2)

HSP 1 Score: 67.3958 bits (163), Expect = 9.789e-16
Identity = 43/124 (34.68%), Postives = 64/124 (51.61%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E       M  +YRV +   + E+        C  +L L++ HL+P AS+ ES   YL M GD++    +  T   +     +S   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   62 RVISSIEQKTEGSERKQQMAKEYRVKVEKELREI--------CYDVLGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFS 177

HSP 2 Score: 37.3502 bits (85), Expect = 9.789e-16
Identity = 21/56 (37.50%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    ++    +L+ ++R  LS AY NV+G   +SW+
Sbjct:    9 VQRAKLAEQAERYDDMAAAMKEVTET--GVELSNEERNLLSVAYKNVVGARRSSWR 62

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 14311_ARATH (14-3-3-like protein GF14 omicron OS=Arabidopsis thaliana GN=GRF11 PE=2 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 9.797e-16
Identity = 45/126 (35.71%), Postives = 67/126 (53.17%), Query Frame = 1

Query:  196 KIISSIDQNEESMGF--------DYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +I+SSI+Q EES G         DYR  +   + ++        C  IL++I+ HL+P A+S ES   Y  M GD+     +F++   + + A  SL  Y++    A  +L+ T PIRLGLALN S
Sbjct:   62 RILSSIEQKEESKGNEQNAKRIKDYRTKVEEELSKI--------CYDILAVIDKHLVPFATSGESTVFYYKMKGDYFRYLAEFKSGADREEAADLSLKAYEAATSSASTELSTTHPIRLGLALNFS 179

HSP 2 Score: 39.2762 bits (90), Expect = 9.797e-16
Identity = 23/56 (41.07%), Postives = 31/56 (55.36%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            VY+AK+ +Q   Y +M E M   A      +LT ++R  LS  Y NVIG   ASW+
Sbjct:    9 VYLAKLNEQAERYDEMVEAMKKVAALDV--ELTIEERNLLSVGYKNVIGARRASWR 62

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433B_CHICK (14-3-3 protein beta/alpha OS=Gallus gallus GN=YWHAB PE=2 SV=1)

HSP 1 Score: 64.6994 bits (156), Expect = 1.273e-15
Identity = 41/124 (33.06%), Postives = 65/124 (52.42%), Query Frame = 1

Query:  196 KIISSIDQNEE------SMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E       MG +YR  +   + ++        C+ +L L++ +L+ +A+  ES   YL M GD++    +  +   K  T  NS   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   60 RVISSIEQKTERNEKKQQMGREYREKIEAELQDI--------CNDVLELLDKYLIVNATQPESKVFYLKMKGDYYRYLSEVASGDNKQTTVANSQQAYQEAFEISKKEMQPTHPIRLGLALNFS 175

HSP 2 Score: 39.6614 bits (91), Expect = 1.273e-15
Identity = 22/56 (39.29%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +  H  +L+ ++R  LS AY NV+G   +SW+
Sbjct:    7 VQKAKLAEQAERYDDMAAAMKAVTEQGH--ELSNEERNLLSVAYKNVVGARRSSWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 14332_ENTHI (14-3-3 protein 2 OS=Entamoeba histolytica PE=3 SV=1)

HSP 1 Score: 62.3882 bits (150), Expect = 1.656e-15
Identity = 41/118 (34.75%), Postives = 61/118 (51.69%), Query Frame = 1

Query:  196 KIISSIDQNEESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +IISS++Q E++ G   RV L               CD +L +I   LL +++S+ES   +  M+GD++    +F     + + A  SL  Y+   + A   L PT PIRLGLALN S
Sbjct:   61 RIISSLEQKEQAKGNTQRVELIKTYRAKIEQELSQKCDDVLKIITEFLLKNSTSIESKVFFKKMEGDYYRYYAEFTVDEKRKEVADKSLAAYQEATDTA-ASLVPTHPIRLGLALNFS 177

HSP 2 Score: 41.5874 bits (96), Expect = 1.656e-15
Identity = 20/56 (35.71%), Postives = 35/56 (62.50%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            VY++K+A+Q   Y +M ++M   A+     +L+ ++R  +S AY NV+G   ASW+
Sbjct:    8 VYLSKLAEQSERYEEMVQYMKQVAEM--GTELSVEERNLISVAYKNVVGSRRASWR 61

The following BLAST results are available for this feature:

BLAST of EY665446 vs. ExPASy Swiss-Prot

BLAST of EY665446 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 130


Match Name	E-value	Identity	Description
1433C_SOYBN	2.634e-16	32.54	14-3-3-like protein C OS=Glycine max GN=GF14C PE=2...	[more]
1433B_PONAB	2.638e-16	33.87	14-3-3 protein beta/alpha OS=Pongo abelii GN=YWHAB...	[more]
1433B_MACFA	2.638e-16	33.87	14-3-3 protein beta/alpha OS=Macaca fascicularis G...	[more]
1433B_HUMAN	2.638e-16	33.87	14-3-3 protein beta/alpha OS=Homo sapiens GN=YWHAB...	[more]
1433B_BOVIN	2.638e-16	33.87	14-3-3 protein beta/alpha OS=Bos taurus GN=YWHAB P...	[more]
1433_FUCVE	9.781e-16	40.24	14-3-3-like protein OS=Fucus vesiculosus PE=2 SV=1	[more]
1433Z_BOMMO	9.789e-16	34.68	14-3-3 protein zeta OS=Bombyx mori GN=14-3-3zeta P...	[more]
14311_ARATH	9.797e-16	35.71	14-3-3-like protein GF14 omicron OS=Arabidopsis th...	[more]
1433B_CHICK	1.273e-15	33.06	14-3-3 protein beta/alpha OS=Gallus gallus GN=YWHA...	[more]
14332_ENTHI	1.656e-15	34.75	14-3-3 protein 2 OS=Entamoeba histolytica PE=3 SV=...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-102-002-B09-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY665446 ID=EY665446; Name=EY665446; organism=Citrus sinensis; type=EST; length=843bp
CGCCTAATGAGTCTTCTCCCTTATGGGATGTCTACATGGCCAAGATGGCC
GACCAGGTTGTCCTTTATTGGGATATGGCTGAGTTCATGCACCATTTCGC
AAAATCAACCCATGCTGATGACCTAACCTGGGATGACCGGAAACATCTCT
CATTTGCTTACAACAATGTGATTGGGGTGGGAATGGCTTCATGGAAAGAT
CATCTCTTCTATTGATCAGAATGAGGAGAGCATGGGATTCGATTACCGTG
TCTTCTTGAATTAAGGAGTACTAGAGATGGATGGAAATTGACCTCTCCCT
CATTGTGATGGGATTCTTAGCCTCATTGAATCTCATCTCCTTCCCTCTGC
CTCTTCGGTTGAGTCTAATGCGCTCTACCTTCCTATGGATGGTGATCACC
ACTTGGATCCGTTTCAGTTTGAGACTGCACCTTTTAAGACCGATACTGCG
CCGAACTCTTTGCCGGACTACAAGTCAACTCACGAAATCGCTATACCTCA
ATTGGCCCCTACTCCCCCCATACGGCTGGGACTGGCACTCAATGTCTCCT
GTGTTCGACTATGACATCCGTTGATCCCCCGATTTTGGATGGCATCCTTG
CAAAGCAAGCTTTTGATTGACCGCCTTTTCTGAGCTTGATAACCTTGGGT
GAAAGATCATGACTAGGAATTTTCTTTGGTATATAACTTTCTCTCGAAAA
AAATCTAACCCTTTAGGACATCTTGTAATCACTGGTGATGGTGGGAAATA
AATTACCGGAAAACAAAAACTAATCCTGAATTCAGGGGAAAACCGGACAA
CTGCTTGAAGTATTGATTTCCCGGTCCTACGATTTGTTACCCG