EY665446 | Citrus Genome Database

Overview


Name	EY665446
Unique Name	EY665446
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	843

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433B_MOUSE (14-3-3 protein beta/alpha OS=Mus musculus GN=Ywhab PE=1 SV=3)

HSP 1 Score: 63.929 bits (154), Expect = 2.149e-15
Identity = 41/124 (33.06%), Postives = 64/124 (51.61%), Query Frame = 1

Query:  196 KIISSIDQNEE------SMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E       MG +YR  +   + ++        C+ +L L++ +L+ +A+  ES   YL M GD+     +  +   K  T  NS   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   62 RVISSIEQKTERNEKKQQMGKEYREKIEAELQDI--------CNDVLELLDKYLILNATQAESKVFYLKMKGDYFRYLSEVASGENKQTTVSNSQQAYQEAFEISKKEMQPTHPIRLGLALNFS 177

HSP 2 Score: 39.6614 bits (91), Expect = 2.149e-15
Identity = 22/56 (39.29%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +  H  +L+ ++R  LS AY NV+G   +SW+
Sbjct:    9 VQKAKLAEQAERYDDMAAAMKAVTEQGH--ELSNEERNLLSVAYKNVVGARRSSWR 62

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433B_RAT (14-3-3 protein beta/alpha OS=Rattus norvegicus GN=Ywhab PE=1 SV=3)

HSP 1 Score: 63.1586 bits (152), Expect = 3.630e-15
Identity = 41/124 (33.06%), Postives = 63/124 (50.81%), Query Frame = 1

Query:  196 KIISSIDQNEE------SMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E       MG +YR  +   + ++        C  +L L++ +L+ +A+  ES   YL M GD+     +  +   K  T  NS   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   62 RVISSIEQKTERNEKKQQMGKEYREKIEAELQDI--------CSDVLELLDKYLILNATHAESKVFYLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKKEMQPTHPIRLGLALNFS 177

HSP 2 Score: 39.6614 bits (91), Expect = 3.630e-15
Identity = 22/56 (39.29%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +  H  +L+ ++R  LS AY NV+G   +SW+
Sbjct:    9 VQKAKLAEQAERYDDMAAAMKAVTEQGH--ELSNEERNLLSVAYKNVVGARRSSWR 62

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433S_HUMAN (14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 4.715e-15
Identity = 40/123 (32.52%), Postives = 61/123 (49.59%), Query Frame = 1

Query:  196 KIISSIDQNE-----ESMGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +++SSI+Q       E  G + R +      E+ G      CD +L L++SHL+  A   ES   YL M GD++    +  T   K     ++   Y+   +I+  ++ PT PIRLGLALN S
Sbjct:   60 RVLSSIEQKSNEEGSEEKGPEVREYREKVETELQGV-----CDTVLGLLDSHLIKEAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFS 177

HSP 2 Score: 40.4318 bits (93), Expect = 4.715e-15
Identity = 21/56 (37.50%), Postives = 33/56 (58.93%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +  AK+A+Q   Y DMA FM    +    ++L+ ++R  LS AY NV+G   A+W+
Sbjct:    7 IQKAKLAEQAERYEDMAAFMKGAVEK--GEELSCEERNLLSVAYKNVVGGQRAAWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433Z_CHICK (14-3-3 protein zeta OS=Gallus gallus GN=YWHAZ PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.035e-14
Identity = 41/124 (33.06%), Postives = 62/124 (50.00%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +++SSI+Q  E       M  +YR  +   + ++        C+ +LSL+E  L+P+AS  ES   YL M GD++    +      K      S   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   60 RVVSSIEQKTEGAEKKQQMAREYREKIETELRDI--------CNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVEQSQQAYQEAFEISKKEMQPTHPIRLGLALNFS 175

HSP 2 Score: 37.3502 bits (85), Expect = 1.035e-14
Identity = 21/56 (37.50%), Postives = 31/56 (55.36%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +     +L+ ++R  LS AY NV+G   +SW+
Sbjct:    7 VQKAKLAEQAERYDDMASCMKSVTEQ--GAELSNEERNLLSVAYKNVVGARRSSWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 143B2_DANRE (14-3-3 protein beta/alpha-2 OS=Danio rerio GN=ywhab2 PE=2 SV=1)

HSP 1 Score: 64.3142 bits (155), Expect = 1.036e-14
Identity = 41/124 (33.06%), Postives = 62/124 (50.00%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            ++ISSI+Q  E       M  +YR  +   + ++        C  +L L+E +L+ +AS  ES   YL M GD++    +  +   K  T  NS   Y+   +I+   + PT PIRLGLALN S
Sbjct:   60 RVISSIEQKTEGNEKKQQMAREYREKIETELQDI--------CSDVLGLLEKYLIANASQAESKVFYLKMKGDYYRYLSEVASGDSKATTVENSQKAYQDAFDISKKDMQPTHPIRLGLALNFS 175

HSP 2 Score: 36.965 bits (84), Expect = 1.036e-14
Identity = 22/56 (39.29%), Postives = 32/56 (57.14%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M   A +    +L+ ++R  LS AY NV+G   +SW+
Sbjct:    7 VQKAKLAEQAERYDDMAAAMK--AVTEGGVELSNEERNLLSVAYKNVVGARRSSWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433S_SHEEP (14-3-3 protein sigma OS=Ovis aries GN=SFN PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.344e-14
Identity = 41/123 (33.33%), Postives = 64/123 (52.03%), Query Frame = 1

Query:  196 KIISSIDQ--NEESM---GFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +++SSI+Q  NEES    G + + +      E+ G      CD +L L+++HL+  A   ES   YL M GD++    +  T   K     ++   Y+   +I+  ++ PT PIRLGLALN S
Sbjct:   60 RVLSSIEQKSNEESSEEKGPEVQEYREKVETELRGV-----CDTVLGLLDTHLIKEAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFS 177

HSP 2 Score: 40.817 bits (94), Expect = 1.344e-14
Identity = 21/56 (37.50%), Postives = 33/56 (58.93%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +  AK+A+Q   Y DMA FM    +    ++L+ ++R  LS AY NV+G   A+W+
Sbjct:    7 IQKAKLAEQAERYEDMAAFMKSAVEK--GEELSCEERNLLSVAYKNVVGGQRAAWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433S_MOUSE (14-3-3 protein sigma OS=Mus musculus GN=Sfn PE=1 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.344e-14
Identity = 31/82 (37.80%), Postives = 45/82 (54.88%), Query Frame = 1

Query:  304 CDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            CD +L L++SHL+  A   ES   YL M GD++    +  T   K     ++   Y+   +I+  ++ PT PIRLGLALN S
Sbjct:   96 CDTVLGLLDSHLIKGAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFS 177

HSP 2 Score: 40.817 bits (94), Expect = 1.344e-14
Identity = 21/56 (37.50%), Postives = 33/56 (58.93%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +  AK+A+Q   Y DMA FM    +    ++L+ ++R  LS AY NV+G   A+W+
Sbjct:    7 IQKAKLAEQAERYEDMAAFMKSAVEK--GEELSCEERNLLSVAYKNVVGGQRAAWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433S_BOVIN (14-3-3 protein sigma OS=Bos taurus GN=SFN PE=2 SV=1)

HSP 1 Score: 60.077 bits (144), Expect = 1.344e-14
Identity = 41/123 (33.33%), Postives = 64/123 (52.03%), Query Frame = 1

Query:  196 KIISSIDQ--NEESM---GFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +++SSI+Q  NEES    G + + +      E+ G      CD +L L+++HL+  A   ES   YL M GD++    +  T   K     ++   Y+   +I+  ++ PT PIRLGLALN S
Sbjct:   60 RVLSSIEQKSNEESSEEKGPEVQEYREKVETELRGV-----CDTVLGLLDTHLIKEAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFS 177

HSP 2 Score: 40.817 bits (94), Expect = 1.344e-14
Identity = 21/56 (37.50%), Postives = 33/56 (58.93%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            +  AK+A+Q   Y DMA FM    +    ++L+ ++R  LS AY NV+G   A+W+
Sbjct:    7 IQKAKLAEQAERYEDMAAFMKSAVEK--GEELSCEERNLLSVAYKNVVGGQRAAWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433Z_PONAB (14-3-3 protein zeta/delta OS=Pongo abelii GN=YWHAZ PE=2 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.345e-14
Identity = 41/124 (33.06%), Postives = 62/124 (50.00%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +++SSI+Q  E       M  +YR  +   + ++        C+ +LSL+E  L+P+AS  ES   YL M GD++    +      K      S   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   60 RVVSSIEQKTEGAEKKQQMAREYREKIETELRDI--------CNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFS 175

HSP 2 Score: 36.965 bits (84), Expect = 1.345e-14
Identity = 21/56 (37.50%), Postives = 31/56 (55.36%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +     +L+ ++R  LS AY NV+G   +SW+
Sbjct:    7 VQKAKLAEQAERYDDMAACMKSVTEQ--GAELSNEERNLLSVAYKNVVGARRSSWR 60

[Close]

BLAST of EY665446 vs. ExPASy Swiss-Prot
Match: 1433Z_HUMAN (14-3-3 protein zeta/delta OS=Homo sapiens GN=YWHAZ PE=1 SV=1)

HSP 1 Score: 63.929 bits (154), Expect = 1.345e-14
Identity = 41/124 (33.06%), Postives = 62/124 (50.00%), Query Frame = 1

Query:  196 KIISSIDQNEES------MGFDYRVFLN*GVLEMDGN*PLPHCDGILSLIESHLLPSASSVESNALYLPMDGDHHLDPFQFETAPFKTDTAPNSLPDYKSTHEIAIPQLAPTPPIRLGLALNVS 549
            +++SSI+Q  E       M  +YR  +   + ++        C+ +LSL+E  L+P+AS  ES   YL M GD++    +      K      S   Y+   EI+  ++ PT PIRLGLALN S
Sbjct:   60 RVVSSIEQKTEGAEKKQQMAREYREKIETELRDI--------CNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFS 175

HSP 2 Score: 36.965 bits (84), Expect = 1.345e-14
Identity = 21/56 (37.50%), Postives = 31/56 (55.36%), Query Frame = 3

Query:   30 VYMAKMADQVVLYWDMAEFMHHFAKSTHADDLTWDDRKHLSFAYNNVIGVGMASWK 197
            V  AK+A+Q   Y DMA  M    +     +L+ ++R  LS AY NV+G   +SW+
Sbjct:    7 VQKAKLAEQAERYDDMAACMKSVTEQ--GAELSNEERNLLSVAYKNVVGARRSSWR 60

The following BLAST results are available for this feature:

BLAST of EY665446 vs. ExPASy Swiss-Prot

BLAST of EY665446 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 130


Match Name	E-value	Identity	Description
1433B_MOUSE	2.149e-15	33.06	14-3-3 protein beta/alpha OS=Mus musculus GN=Ywhab...	[more]
1433B_RAT	3.630e-15	33.06	14-3-3 protein beta/alpha OS=Rattus norvegicus GN=...	[more]
1433S_HUMAN	4.715e-15	32.52	14-3-3 protein sigma OS=Homo sapiens GN=SFN PE=1 S...	[more]
1433Z_CHICK	1.035e-14	33.06	14-3-3 protein zeta OS=Gallus gallus GN=YWHAZ PE=2...	[more]
143B2_DANRE	1.036e-14	33.06	14-3-3 protein beta/alpha-2 OS=Danio rerio GN=ywha...	[more]
1433S_SHEEP	1.344e-14	33.33	14-3-3 protein sigma OS=Ovis aries GN=SFN PE=2 SV=...	[more]
1433S_MOUSE	1.344e-14	37.80	14-3-3 protein sigma OS=Mus musculus GN=Sfn PE=1 S...	[more]
1433S_BOVIN	1.344e-14	33.33	14-3-3 protein sigma OS=Bos taurus GN=SFN PE=2 SV=...	[more]
1433Z_PONAB	1.345e-14	33.06	14-3-3 protein zeta/delta OS=Pongo abelii GN=YWHAZ...	[more]
1433Z_HUMAN	1.345e-14	33.06	14-3-3 protein zeta/delta OS=Homo sapiens GN=YWHAZ...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-102-002-B09-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY665446 ID=EY665446; Name=EY665446; organism=Citrus sinensis; type=EST; length=843bp
CGCCTAATGAGTCTTCTCCCTTATGGGATGTCTACATGGCCAAGATGGCC
GACCAGGTTGTCCTTTATTGGGATATGGCTGAGTTCATGCACCATTTCGC
AAAATCAACCCATGCTGATGACCTAACCTGGGATGACCGGAAACATCTCT
CATTTGCTTACAACAATGTGATTGGGGTGGGAATGGCTTCATGGAAAGAT
CATCTCTTCTATTGATCAGAATGAGGAGAGCATGGGATTCGATTACCGTG
TCTTCTTGAATTAAGGAGTACTAGAGATGGATGGAAATTGACCTCTCCCT
CATTGTGATGGGATTCTTAGCCTCATTGAATCTCATCTCCTTCCCTCTGC
CTCTTCGGTTGAGTCTAATGCGCTCTACCTTCCTATGGATGGTGATCACC
ACTTGGATCCGTTTCAGTTTGAGACTGCACCTTTTAAGACCGATACTGCG
CCGAACTCTTTGCCGGACTACAAGTCAACTCACGAAATCGCTATACCTCA
ATTGGCCCCTACTCCCCCCATACGGCTGGGACTGGCACTCAATGTCTCCT
GTGTTCGACTATGACATCCGTTGATCCCCCGATTTTGGATGGCATCCTTG
CAAAGCAAGCTTTTGATTGACCGCCTTTTCTGAGCTTGATAACCTTGGGT
GAAAGATCATGACTAGGAATTTTCTTTGGTATATAACTTTCTCTCGAAAA
AAATCTAACCCTTTAGGACATCTTGTAATCACTGGTGATGGTGGGAAATA
AATTACCGGAAAACAAAAACTAATCCTGAATTCAGGGGAAAACCGGACAA
CTGCTTGAAGTATTGATTTCCCGGTCCTACGATTTGTTACCCG