CV884745

Overview
NameCV884745
Unique NameCV884745
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length755
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Library UCRCS04-UCRcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig4591 contig Csv1_Contig4591:53..809. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CV884745 vs. ExPASy Swiss-Prot
Match: UB2D1_BOVIN (Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus GN=UBE2D1 PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 8.925e-33
Identity = 69/143 (48.25%), Postives = 94/143 (65.73%), Query Frame = -3 
Query:  223 RRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYA 651
            +RI KE   L  +P    SA P  D++ ++   I+GP  S Y+GGVF L +  P +YP   PK+ F TKIYHPNI+  G ICLDIL+ +WSPAL +  VLLSI +LL  PNPDDPL  +IA+ +K+++ +    A+EWT+ YA
Sbjct:    4 KRIQKELSDLQRDPPAHCSAGPVGDDLFHWQATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTVSKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYA 146
[Close]
BLAST of CV884745 vs. ExPASy Swiss-Prot
Match: UB2E1_MOUSE (Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus GN=Ube2e1 PE=2 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 1.166e-32
Identity = 75/148 (50.68%), Postives = 92/148 (62.16%), Query Frame = -3 
Query:  220 SNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYAS 663
            S   +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    EYP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP DPL  +IA  + TN AE    A++WT+ YA+
Sbjct:   46 STSAKRIQKELADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT 193
[Close]
BLAST of CV884745 vs. ExPASy Swiss-Prot
Match: UB2E1_HUMAN (Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens GN=UBE2E1 PE=1 SV=1)

HSP 1 Score: 140.198 bits (352), Expect = 1.166e-32
Identity = 75/148 (50.68%), Postives = 92/148 (62.16%), Query Frame = -3 
Query:  220 SNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYAS 663
            S   +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    EYP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP DPL  +IA  + TN AE    A++WT+ YA+
Sbjct:   46 STSAKRIQKELADITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFTPEYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT 193
[Close]
BLAST of CV884745 vs. ExPASy Swiss-Prot
Match: UB2E3_MOUSE (Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus GN=Ube2e3 PE=1 SV=2)

HSP 1 Score: 139.813 bits (351), Expect = 1.522e-32
Identity = 74/148 (50.00%), Postives = 92/148 (62.16%), Query Frame = -3 
Query:  220 SNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYAS 663
            S   +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    +YP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP DPL  +IA  + TN AE    A++WT+ YA+
Sbjct:   60 STSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYAT 207
[Close]
BLAST of CV884745 vs. ExPASy Swiss-Prot
Match: UB2E3_HUMAN (Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens GN=UBE2E3 PE=1 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 1.522e-32
Identity = 74/148 (50.00%), Postives = 92/148 (62.16%), Query Frame = -3 
Query:  220 SNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYAS 663
            S   +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    +YP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP DPL  +IA  + TN AE    A++WT+ YA+
Sbjct:   60 STSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYAT 207
[Close]
BLAST of CV884745 vs. ExPASy Swiss-Prot
Match: UB2E3_BOVIN (Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus GN=UBE2E3 PE=2 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 1.522e-32
Identity = 74/148 (50.00%), Postives = 92/148 (62.16%), Query Frame = -3 
Query:  220 SNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYAS 663
            S   +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    +YP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP DPL  +IA  + TN AE    A++WT+ YA+
Sbjct:   60 STSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSSDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYLTNRAEHDRIARQWTKRYAT 207
[Close]
BLAST of CV884745 vs. ExPASy Swiss-Prot
Match: UB2E2_MOUSE (Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus GN=Ube2e2 PE=2 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 1.522e-32
Identity = 74/148 (50.00%), Postives = 92/148 (62.16%), Query Frame = -3 
Query:  220 SNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYAS 663
            S   +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    +YP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP DPL  +IA  + TN AE    A++WT+ YA+
Sbjct:   54 STSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT 201
[Close]
BLAST of CV884745 vs. ExPASy Swiss-Prot
Match: UB2E2_HUMAN (Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens GN=UBE2E2 PE=1 SV=1)

HSP 1 Score: 139.813 bits (351), Expect = 1.522e-32
Identity = 74/148 (50.00%), Postives = 92/148 (62.16%), Query Frame = -3 
Query:  220 SNLPRRIIKETQRLLSEPAPGISASPSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYAS 663
            S   +RI KE   +  +P P  SA P  DN+  +   ILGP  S YEGGVF L++    +YP   PKV F T+IYH NI+  G ICLDILKD WSPAL I  VLLSI +LL+  NP DPL  +IA  + TN AE    A++WT+ YA+
Sbjct:   54 STSAKRIQKELAEITLDPPPNCSAGPKGDNIYEWRSTILGPPGSVYEGGVFFLDITFSPDYPFKPPKVTFRTRIYHCNINSQGVICLDILKDNWSPALTISKVLLSICSLLTDCNPADPLVGSIATQYMTNRAEHDRMARQWTKRYAT 201
[Close]
BLAST of CV884745 vs. ExPASy Swiss-Prot
Match: UBC12_ARATH (Probable ubiquitin-conjugating enzyme E2 12 OS=Arabidopsis thaliana GN=UBC12 PE=2 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.289e-31
Identity = 67/146 (45.89%), Postives = 95/146 (65.07%), Query Frame = -3 
Query:  217 RRIIKETQRLLSEPAPGISASP-SEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKLGRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYASG 651
            +RI +E + +   P    SA P +E+++ ++   I+GP  SPY GGVF + +    +YP   PKV F TK+YHPNID  G ICLDILK++WSPA     VLLSI +LL+ PNP+DPL   IA  +K ++++   TA++WT+ YA G
Sbjct:    4 KRISRELRDMQRHPPANCSAGPVAEEDIFHWQATIMGPHDSPYSGGVFTVSIDFSSDYPFKPPKVNFKTKVYHPNIDSKGSICLDILKEQWSPAPTTSKVLLSICSLLTDPNPNDPLVPEIAHLYKVDKSKYESTAQKWTQKYAMG 149
[Close]
BLAST of CV884745 vs. ExPASy Swiss-Prot
Match: UBC1_YEAST (Ubiquitin-conjugating enzyme E2-24 kDa OS=Saccharomyces cerevisiae GN=UBC1 PE=1 SV=1)

HSP 1 Score: 134.035 bits (336), Expect = 8.354e-31
Identity = 65/146 (44.52%), Postives = 96/146 (65.75%), Query Frame = -3 
Query:  220 RRIIKETQRLLSEPAPGISAS-PSEDNMRYFNVMILGPTQSPYEGGVFKLELFLPEEYPMAAPKVRFLTKIYHPNIDKL-GRICLDILKDKWSPALQIRTVLLSIQALLSAPNPDDPLSENIAKHWKTNEAEAVETAKEWTRLYAS 651
            +RI+KE Q +  +PA  I+    SE ++ +     LGP  +PYEGG F +++ +P EYP   PK++F TK+YHPNI  + G ICLDILK+ WSP + +++ L+S+QALL +P P+DP    +A+H+  +     +TA  WTRLYAS
Sbjct:    5 KRIMKEIQAVKDDPAAHITLEFVSESDIHHLKGTFLGPPGTPYEGGKFVVDIEVPMEYPFKPPKMQFDTKVYHPNISSVTGAICLDILKNAWSPVITLKSALISLQALLQSPEPNDPQDAEVAQHYLRDRESFNKTAALWTRLYAS 150
The following BLAST results are available for this feature:
BLAST of CV884745 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 250
Match NameE-valueIdentityDescription
UB2D1_BOVIN8.925e-3348.25Ubiquitin-conjugating enzyme E2 D1 OS=Bos taurus G... [more]
UB2E1_MOUSE1.166e-3250.68Ubiquitin-conjugating enzyme E2 E1 OS=Mus musculus... [more]
UB2E1_HUMAN1.166e-3250.68Ubiquitin-conjugating enzyme E2 E1 OS=Homo sapiens... [more]
UB2E3_MOUSE1.522e-3250.00Ubiquitin-conjugating enzyme E2 E3 OS=Mus musculus... [more]
UB2E3_HUMAN1.522e-3250.00Ubiquitin-conjugating enzyme E2 E3 OS=Homo sapiens... [more]
UB2E3_BOVIN1.522e-3250.00Ubiquitin-conjugating enzyme E2 E3 OS=Bos taurus G... [more]
UB2E2_MOUSE1.522e-3250.00Ubiquitin-conjugating enzyme E2 E2 OS=Mus musculus... [more]
UB2E2_HUMAN1.522e-3250.00Ubiquitin-conjugating enzyme E2 E2 OS=Homo sapiens... [more]
UBC12_ARATH1.289e-3145.89Probable ubiquitin-conjugating enzyme E2 12 OS=Ara... [more]
UBC1_YEAST8.354e-3144.52Ubiquitin-conjugating enzyme E2-24 kDa OS=Saccharo... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionUCRCS04_2_010C10_T7 Ruby Orange Developing Flower cDNA Library UCRCS04-UCR Citrus sinensis cDNA clone UCRCS04_010_T7_C10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CV884745 ID=CV884745; Name=CV884745; organism=Citrus sinensis; type=EST; length=755bp
TGTATATCATCTAATCATACAGTTCAAAGACATGGCTTCCACAAGAAGAA
AATAATTGACATGTGCGGTAAGATGGAAGCTTTCAGAATGTCAAACTCCG
AATGTTCAAACATCAATTCAATTCTTCCAAATTTTCCATTTTAAACAGAC
AGTGAATAGCATACATTGCACAATTGTTATTTGAATGAAGTGATATTTTC
CACCATGTCATCAAGCACCACTGGCATATAAACGTGTCCATTCCTTCGCT
GTTTCAACTGCTTCGGCCTCATTTGTTTTCCAGTGTTTGGCAATGTTCTC
GGAGAGAGGATCATCGGGGTTAGGAGCACTCAGAAGTGCTTGAATGCTCA
ACAGTACAGTCCGAATCTGGAGAGCTGGACTCCACTTGTCTTTCAGAATA
TCAAGACAAATCCTTCCAAGCTTATCAATGTTAGGATGATATATCTTGGT
AAGAAATCGGACCTTTGGAGCAGCCATTGGATATTCTTCAGGCAAAAATA
ATTCCAATTTGAACACCCCTCCTTCATATGGAGATTGCGTCGGACCAAGG
ATCATCACATTAAAGTATCGCATATTATCTTCCGAGGGAGATGCACTTAT
TCCTGGTGCTGGTTCACTGAGGAGACGCTGTGTTTCCTTGATGATACGTC
GAGGGAGATTACTGTTCGCCATTTCCAAGATCGATTTTTTTTGTTTTTTA
ACCTTCTTGTTGTTGCAGAGAAAGAACGCTGATAGGGTTTTCTAAGTCTA
ACCCT
back to top