EY679003

Overview
NameEY679003
Unique NameEY679003
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length876
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: C1TC_SCHPO (C-1-tetrahydrofolate synthase, cytoplasmic OS=Schizosaccharomyces pombe GN=SPBC839.16 PE=2 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 2.244e-27
Identity = 51/103 (49.51%), Postives = 72/103 (69.90%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GGKGA+ +  A+  ACENV    + LY +   I++KI+ IA+  YGA G+E S  A++++E +T QG++ LPIC+A TQYS SHD   KGAPT F +PIRD++
Sbjct:  793 GGKGALGVARALINACENVDSEFRLLYDVHEPIEKKIEIIAKEMYGADGIELSPLAKERLETFTKQGYNNLPICIAKTQYSLSHDPDLKGAPTNFTVPIRDMR 895

HSP 2 Score: 40.817 bits (94), Expect = 2.244e-27
Identity = 17/26 (65.38%), Postives = 20/26 (76.92%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGFIYPL   +ST+PGLP +P
Sbjct:  895 RLSAGAGFIYPLAAAISTIPGLPTKP 920
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_ROSCS (Formate--tetrahydrofolate ligase OS=Roseiflexus castenholzii (strain DSM 13941 / HLO8) GN=fhs PE=3 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 5.348e-26
Identity = 52/105 (49.52%), Postives = 71/105 (67.62%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKF--LYPLDVSIKEKIDTIA-RSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG G  DL  A  +ACE    P  F  LYPLD+S++EKI+T+A + YGA+ VEY+ EA +++  +  QG+  LPICMA T  S SHD   +GAP+G++ PIRDV+
Sbjct:  421 GGAGGCDLARAAIQACE---APGTFTPLYPLDMSLREKIETLATKVYGATRVEYTPEASRRLAQFEHQGYGNLPICMAKTHLSISHDPKLRGAPSGYVFPIRDVR 522

HSP 2 Score: 40.817 bits (94), Expect = 5.348e-26
Identity = 18/30 (60.00%), Postives = 20/30 (66.67%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPCLLR 428
            R + GAGFIYPL G + TMPGLP  P   R
Sbjct:  522 RLAAGAGFIYPLAGDIRTMPGLPAHPAAER 551
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_DESRM (Formate--tetrahydrofolate ligase OS=Desulfotomaculum reducens (strain MI-1) GN=fhs PE=3 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 7.456e-25
Identity = 49/104 (47.12%), Postives = 67/104 (64.42%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIA-RSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            GG+GA++L  AV  ACE  T   + LYP ++SIKEKI+ +A + Y A GV +   AE++I+ +   G   LPICMA T  S SHD A KG P  +I PIRD+++
Sbjct:  424 GGEGAIELAEAVVAACEK-TADFQLLYPDNLSIKEKIEVLATKVYNADGVVFEPLAERKIKQFEDLGLGHLPICMAKTHLSISHDPAMKGLPKNYIFPIRDIRA 526

HSP 2 Score: 43.1282 bits (100), Expect = 7.456e-25
Identity = 18/26 (69.23%), Postives = 21/26 (80.77%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            RAS+GAGF+YPL G M TMPGL  +P
Sbjct:  525 RASVGAGFLYPLAGAMRTMPGLGSKP 550
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS2_RUBXD (Formate--tetrahydrofolate ligase 2 OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129) GN=fhs2 PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 2.138e-24
Identity = 50/112 (44.64%), Postives = 70/112 (62.50%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQP--------LKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            GGKGAV+L  A++   E   +          +FLYPL++ +K+KI+TIAR  YGA GVEY  EA + +E +   GF  LP+C+A T  S S D A KGAP G+ L +R+V++
Sbjct:  424 GGKGAVELARALEETIEENERERRRGGGGSFRFLYPLEMPLKQKIETIAREVYGAEGVEYDAEALRALEGFERAGFGRLPVCLAKTHLSLSSDPALKGAPRGWKLSVREVRA 535

HSP 2 Score: 42.743 bits (99), Expect = 2.138e-24
Identity = 19/30 (63.33%), Postives = 21/30 (70.00%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPCLLR 428
            RAS+GAGFIYP+ G M TMPGL   P   R
Sbjct:  534 RASVGAGFIYPICGQMRTMPGLSAHPAAER 563
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS1_RUBXD (Formate--tetrahydrofolate ligase 1 OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129) GN=fhs1 PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 2.138e-24
Identity = 50/112 (44.64%), Postives = 70/112 (62.50%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQP--------LKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            GGKGAV+L  A++   E   +          +FLYPL++ +K+KI+TIAR  YGA GVEY  EA + +E +   GF  LP+C+A T  S S D A KGAP G+ L +R+V++
Sbjct:  424 GGKGAVELARALEETIEENERERRRGGGGSFRFLYPLEMPLKQKIETIAREVYGAEGVEYDAEALRALEGFERAGFGRLPVCLAKTHLSLSSDPALKGAPRGWKLSVREVRA 535

HSP 2 Score: 42.743 bits (99), Expect = 2.138e-24
Identity = 19/30 (63.33%), Postives = 21/30 (70.00%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPCLLR 428
            RAS+GAGFIYP+ G M TMPGL   P   R
Sbjct:  534 RASVGAGFIYPICGQMRTMPGLSAHPAAER 563
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS3_RUBXD (Formate--tetrahydrofolate ligase 3 OS=Rubrobacter xylanophilus (strain DSM 9941 / NBRC 16129) GN=fhs3 PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 2.138e-24
Identity = 50/112 (44.64%), Postives = 70/112 (62.50%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQP--------LKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            GGKGAV+L  A++   E   +          +FLYPL++ +K+KI+TIAR  YGA GVEY  EA + +E +   GF  LP+C+A T  S S D A KGAP G+ L +R+V++
Sbjct:  421 GGKGAVELARALEETIEENERERRRGGGGSFRFLYPLEMPLKQKIETIAREVYGAEGVEYDAEALRALEGFERAGFGRLPVCLAKTHLSLSSDPALKGAPRGWKLSVREVRA 532

HSP 2 Score: 42.743 bits (99), Expect = 2.138e-24
Identity = 19/30 (63.33%), Postives = 21/30 (70.00%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPCLLR 428
            RAS+GAGFIYP+ G M TMPGL   P   R
Sbjct:  531 RASVGAGFIYPICGQMRTMPGLSAHPAAER 560
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: C1TC_SPOFR (C-1-tetrahydrofolate synthase, cytoplasmic OS=Spodoptera frugiperda PE=2 SV=3)

HSP 1 Score: 90.1225 bits (222), Expect = 6.083e-24
Identity = 49/102 (48.04%), Postives = 67/102 (65.69%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDV 337
            GG GA++L  AV  AC++ ++   FLYPL +SI+EKI  IA+  YGA  VEY++E  ++I+ +T  G+   PICMA T  S + D A KGAPTGF L I  +
Sbjct:  789 GGLGALELADAVIEACDSKSK-FDFLYPLQLSIQEKIQIIAKEMYGAGQVEYTDEVLEKIKTFTDMGYDKFPICMAKTSNSLTGDPAVKGAPTGFTLKINGI 889

HSP 2 Score: 42.3578 bits (98), Expect = 6.083e-24
Identity = 17/27 (62.96%), Postives = 22/27 (81.48%), Query Frame = 3 
Query:  342 ASIGAGFIYPLVGTMSTMPGLPIRPCL 422
            AS+GAGF+ P+VG +S MPGLP RP +
Sbjct:  891 ASVGAGFVVPMVGEISKMPGLPTRPSI 917
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_BACHK (Formate--tetrahydrofolate ligase OS=Bacillus thuringiensis subsp. konkukian GN=fhs PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 6.134e-24
Identity = 48/103 (46.60%), Postives = 67/103 (65.05%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG+G VDL   V +  E        LY L++ ++EKI TIA+  YGA  +E++ +A KQ+  Y G+G+S LPICMA TQYS S DA + G P+ FI+ IR++K
Sbjct:  418 GGQGGVDLAEKVLKEIEKGENNYAPLYELELPLEEKIRTIAQKVYGAKDIEFAPKARKQLAQYEGEGWSNLPICMAKTQYSLSDDATKLGRPSDFIVTIRELK 520

HSP 2 Score: 40.817 bits (94), Expect = 6.134e-24
Identity = 19/30 (63.33%), Postives = 22/30 (73.33%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPCLLR 428
            + SIGAGFI  L GTM TMPGLP +P  L+
Sbjct:  520 KPSIGAGFIVALTGTMLTMPGLPKQPAALQ 549
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_BACCZ (Formate--tetrahydrofolate ligase OS=Bacillus cereus (strain ZK / E33L) GN=fhs PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 6.134e-24
Identity = 48/103 (46.60%), Postives = 67/103 (65.05%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG+G VDL   V +  E        LY L++ ++EKI TIA+  YGA  +E++ +A KQ+  Y G+G+S LPICMA TQYS S DA + G P+ FI+ IR++K
Sbjct:  418 GGQGGVDLAEKVLKEIEKGENNYAPLYELELPLEEKIRTIAQKVYGAKDIEFAPKARKQLAQYEGEGWSNLPICMAKTQYSLSDDATKLGRPSDFIVTIRELK 520

HSP 2 Score: 40.817 bits (94), Expect = 6.134e-24
Identity = 19/30 (63.33%), Postives = 22/30 (73.33%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPCLLR 428
            + SIGAGFI  L GTM TMPGLP +P  L+
Sbjct:  520 KPSIGAGFIVALTGTMLTMPGLPKQPAALQ 549
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_BACCQ (Formate--tetrahydrofolate ligase OS=Bacillus cereus (strain Q1) GN=fhs PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 6.134e-24
Identity = 48/103 (46.60%), Postives = 67/103 (65.05%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG+G VDL   V +  E        LY L++ ++EKI TIA+  YGA  +E++ +A KQ+  Y G+G+S LPICMA TQYS S DA + G P+ FI+ IR++K
Sbjct:  418 GGQGGVDLAEKVLKEIEKGENNYAPLYELELPLEEKIRTIAQKVYGAKDIEFAPKARKQLAQYEGEGWSNLPICMAKTQYSLSDDATKLGRPSDFIVTIRELK 520

HSP 2 Score: 40.817 bits (94), Expect = 6.134e-24
Identity = 19/30 (63.33%), Postives = 22/30 (73.33%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPCLLR 428
            + SIGAGFI  L GTM TMPGLP +P  L+
Sbjct:  520 KPSIGAGFIVALTGTMLTMPGLPKQPAALQ 549
The following BLAST results are available for this feature:
BLAST of EY679003 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 293
Match NameE-valueIdentityDescription
C1TC_SCHPO2.244e-2749.51C-1-tetrahydrofolate synthase, cytoplasmic OS=Schi... [more]
FTHS_ROSCS5.348e-2649.52Formate--tetrahydrofolate ligase OS=Roseiflexus ca... [more]
FTHS_DESRM7.456e-2547.12Formate--tetrahydrofolate ligase OS=Desulfotomacul... [more]
FTHS2_RUBXD2.138e-2444.64Formate--tetrahydrofolate ligase 2 OS=Rubrobacter ... [more]
FTHS1_RUBXD2.138e-2444.64Formate--tetrahydrofolate ligase 1 OS=Rubrobacter ... [more]
FTHS3_RUBXD2.138e-2444.64Formate--tetrahydrofolate ligase 3 OS=Rubrobacter ... [more]
C1TC_SPOFR6.083e-2448.04C-1-tetrahydrofolate synthase, cytoplasmic OS=Spod... [more]
FTHS_BACHK6.134e-2446.60Formate--tetrahydrofolate ligase OS=Bacillus thuri... [more]
FTHS_BACCZ6.134e-2446.60Formate--tetrahydrofolate ligase OS=Bacillus cereu... [more]
FTHS_BACCQ6.134e-2446.60Formate--tetrahydrofolate ligase OS=Bacillus cereu... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-053-H07-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY679003 ID=EY679003; Name=EY679003; organism=Citrus sinensis; type=EST; length=876bp
GCCAGTTTAGTTTTCGTTCTCACCATCGCCCATGGGTGGAAAAGGAGCAG
TGGACCTTGGAATTGCAGTTCAAAGAGCCTGTGAGAATGTTACACAGCCA
TTGAAGTTCTTATATCCTTTAGATGTCAGTATTAAAGAGAAAATAGATAC
AATAGCAAGGTCATATGGTGCTAGCGGCGTTGAGTACTCAGAGGAGGCTG
AGAAACAGATCGAAATGTACACCGGGCAAGGGTTTTCTGGCTTACCAATT
TGCATGGCATAAACCCAGTATTCATTTTCACACGATGCCGCCGAGAAGGG
AGCCCCAACTGGGTTTATCTTACCGATAAGGGATGTAAAGAGCTAGCATT
GGGGCTGGATTCATTTATCCTTTGGTTGGAACAATGAGTACAATGCCAGG
TCTCCCAATAAGGCCATGCCTTTTAAGAGAATGGAGTCAACACTGCAACT
GGAAAGGTTGTTGGGGCTGTCTTAAGATCCCACAAAGCACTCCCGTGTGG
GTCCTCAAGAAGCTCTCCTTTAGATGTTCTTAAATGCTCCTGAGCAAACA
ATGAGCTTGAAGGGCTCCCCCCCCCCCCCACTGGCTGATATTCTAACCCC
GATCTGTAACACTTATGGGTACCTCGGGCTATTTGCTGTGGTAAACAAAA
CCCGACACCAAATAGAACCTGGAAAAAAAAAATCTTGGTACTCACCTTGC
GGACTTTAATTTTCCCTATTAATGTGTCCTTACTCCCATTTGATCTTGAA
TCCCGTTACGAAAATTTACCCTATGGGTGGGGGTGTGATATTTGTAAAAT
AAACAAAAAAAAAAAAAAAATAAAATAAAAGCAAAAGGGCGCGGGCGCGG
GTTAATAAATTATGAAAGGTATCAGA
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