EY679003

Overview
NameEY679003
Unique NameEY679003
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length876
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_SHELP (Formate--tetrahydrofolate ligase OS=Shewanella loihica (strain ATCC BAA-1088 / PV-4) GN=fhs PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 5.320e-18
Identity = 46/103 (44.66%), Postives = 63/103 (61.17%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            G +GA+ L  AV RA E  +Q  ++LY    SI+ K+ TIA + YGA+G+  S+ A+ Q+     QGF  L +C+A T  S SHD   KGAP GF LPI ++K
Sbjct:  421 GAEGAMALARAVVRATEQPSQ-FRYLYETKQSIEAKLLTIAEAGYGANGISLSDTAKAQLAQLQRQGFDKLALCIAKTPMSVSHDPKLKGAPQGFELPIAELK 522

HSP 2 Score: 34.6538 bits (78), Expect = 5.320e-18
Identity = 16/26 (61.54%), Postives = 19/26 (73.08%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            + + GAGFI  LVG + TMPGL IRP
Sbjct:  522 KLNAGAGFITALVGKVMTMPGLGIRP 547
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_ALKOO (Formate--tetrahydrofolate ligase OS=Alkaliphilus oremlandii (strain OhILAs) GN=fhs PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 5.322e-18
Identity = 41/103 (39.81%), Postives = 64/103 (62.14%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG G +++   V    E+     K LY ++ SI EKI+TIA+  YGA GV++++ A+ QI+ Y   G   +PICMA TQYS S D +  G P+GF + +++++
Sbjct:  412 GGDGGIEMAKKVVEVVESKESNFKPLYDVNASIVEKINTIAKEVYGADGVDFTKSAQTQIKKYEDLGLDKMPICMAKTQYSLSDDPSLIGRPSGFRITVKEIR 514

HSP 2 Score: 30.8018 bits (68), Expect = 5.322e-18
Identity = 14/26 (53.85%), Postives = 16/26 (61.54%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGF+  L G +  MPGLP  P
Sbjct:  514 RLSAGAGFLVALTGDIMVMPGLPKVP 539
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_AERHH (Formate--tetrahydrofolate ligase OS=Aeromonas hydrophila subsp. hydrophila (strain ATCC 7966 / NCIB 9240) GN=fhs PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 8.989e-18
Identity = 44/103 (42.72%), Postives = 62/103 (60.19%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIAR-SYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG GA +L  AV  ACE   + +K LYP   S+  K+ T+    YG  GV+ S++A +Q+   + +G+  LP+C+A T  S SHD A KG PT F +PI +VK
Sbjct:  433 GGAGASELAQAVVAACEQPGK-VKLLYPDQASLGAKLATLVECGYGGRGVQLSDKARQQLAQLSAEGWDHLPVCVAKTPLSISHDPALKGVPTDFEVPIEEVK 534

HSP 2 Score: 30.8018 bits (68), Expect = 8.989e-18
Identity = 13/22 (59.09%), Postives = 15/22 (68.18%), Query Frame = 3 
Query:  351 GAGFIYPLVGTMSTMPGLPIRP 416
            GAGF+Y L G + TMPGL   P
Sbjct:  538 GAGFVYALAGPIMTMPGLGSLP 559
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_GRABC (Formate--tetrahydrofolate ligase OS=Granulibacter bethesdensis (strain ATCC BAA-1260 / CGDNIH1) GN=fhs PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 8.990e-18
Identity = 44/103 (42.72%), Postives = 62/103 (60.19%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG GA DL   V +  E  +   K LYP D+ + +K+ TIA   YGAS +       K+ +    +GF  LPIC+A TQYSFS DA  +GAP+G ++P+RD++
Sbjct:  428 GGAGAADLAHEVVKLVEGGSADFKPLYPEDMPLWDKLRTIATEIYGASDITADAAVRKRFDELQKEGFGHLPICVAKTQYSFSTDANLRGAPSGHVIPVRDLR 530

HSP 2 Score: 28.8758 bits (63), Expect = 8.990e-18
Identity = 13/26 (50.00%), Postives = 16/26 (61.54%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GA F+  + G + TMPGLP  P
Sbjct:  530 RLSAGAEFVVAICGDIMTMPGLPKVP 555
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_SHEAM (Formate--tetrahydrofolate ligase OS=Shewanella amazonensis (strain ATCC BAA-1098 / SB2B) GN=fhs PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 8.994e-18
Identity = 42/98 (42.86%), Postives = 59/98 (60.20%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPI 328
            G +GA  L  AV RAC+      ++LY  ++    ++ T+A  YGA+GV  SE+A + + M T  G   LP+CMA T  S SHD + KGAP+GF LP+
Sbjct:  418 GAEGARALATAVARACDAPVN-FEYLYDDEMDFGARLMTLAERYGAAGVSLSEDARRDLAMITELGMDKLPLCMAKTPLSISHDPSLKGAPSGFELPV 514

HSP 2 Score: 31.5722 bits (70), Expect = 8.994e-18
Identity = 15/26 (57.69%), Postives = 17/26 (65.38%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGFI  L G + TMPGL + P
Sbjct:  518 RLSAGAGFITVLTGKVMTMPGLGLLP 543
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_CLOD6 (Formate--tetrahydrofolate ligase OS=Clostridium difficile (strain 630) GN=fhs PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 8.995e-18
Identity = 42/100 (42.00%), Postives = 62/100 (62.00%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIA-RSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIR 331
            GG+G + +   V R  +      +F Y  D+SIK+KI+ IA + YGA GV+Y+ EA+K+I      GF+ +P+CMA TQYS + D  + G PTGF + +R
Sbjct:  414 GGEGGIAVAKEVLRLLDEEENNFRFCYEDDLSIKDKINAIATKIYGADGVDYTPEADKEIANLEKLGFTKVPVCMAKTQYSLTDDQTKLGRPTGFRITVR 513

HSP 2 Score: 29.6462 bits (65), Expect = 8.995e-18
Identity = 14/24 (58.33%), Postives = 15/24 (62.50%), Query Frame = 3 
Query:  345 SIGAGFIYPLVGTMSTMPGLPIRP 416
            S GAGFI  L G +  MPGLP  P
Sbjct:  518 SAGAGFIVALTGEIMKMPGLPKVP 541
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_METPB (Formate--tetrahydrofolate ligase OS=Methylobacterium populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001) GN=fhs PE=3 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 8.995e-18
Identity = 41/103 (39.81%), Postives = 61/103 (59.22%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIA-RSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG GA +L  AV +  E   +PL F Y  +  + +KI  IA + YGA+ ++   +A  ++  +   G+  LPICMA TQYSFS D    GAP+G ++ +RDV+
Sbjct:  413 GGAGAEELAQAVVKLAEGEQKPLTFAYDTETKLTDKIKAIATKLYGAADIQIESKAATKLAGFEKDGYGHLPICMAKTQYSFSTDPTLMGAPSGHLVSVRDVR 515

HSP 2 Score: 31.9574 bits (71), Expect = 8.995e-18
Identity = 14/26 (53.85%), Postives = 17/26 (65.38%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGF+  + G + TMPGLP  P
Sbjct:  515 RLSAGAGFVVAICGEIMTMPGLPKVP 540
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_AERS4 (Formate--tetrahydrofolate ligase OS=Aeromonas salmonicida (strain A449) GN=fhs PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 1.519e-17
Identity = 45/103 (43.69%), Postives = 63/103 (61.17%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIAR-SYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG GA +L  AV  ACE  +Q ++ LYP ++S+  K+ T+    YG  GV  S++A +Q+   +  G+  LPIC+A T  S SHD A KG PT F +PI +VK
Sbjct:  437 GGAGASELARAVVAACEMPSQ-IQLLYPDEMSLCAKLATLVECGYGGRGVILSDKAHRQLAALSAAGWDHLPICVAKTPLSISHDPARKGVPTDFEVPIDEVK 538

HSP 2 Score: 30.8018 bits (68), Expect = 1.519e-17
Identity = 13/22 (59.09%), Postives = 15/22 (68.18%), Query Frame = 3 
Query:  351 GAGFIYPLVGTMSTMPGLPIRP 416
            GAGF+Y L G + TMPGL   P
Sbjct:  542 GAGFVYALAGPIMTMPGLGSLP 563
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_FINM2 (Formate--tetrahydrofolate ligase OS=Finegoldia magna (strain ATCC 29328) GN=fhs PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.521e-17
Identity = 45/105 (42.86%), Postives = 64/105 (60.95%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQP--LKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG+GA +L    +   EN  +    +FLY L++ IKEKI+ IA+  Y A GVE+S +A+K+++     G    P+CMA TQYSFS +    GAPTGF + + D K
Sbjct:  411 GGEGAKELA---EYVVENTKEENDFEFLYDLELPIKEKIEKIAKEIYRADGVEFSAKAKKKLKQIKELGLDNYPVCMAKTQYSFSDNKKLIGAPTGFTITVSDFK 512

HSP 2 Score: 32.3426 bits (72), Expect = 1.521e-17
Identity = 14/26 (53.85%), Postives = 19/26 (73.08%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            + S GAGF+  L+G++ TMPGLP  P
Sbjct:  512 KISRGAGFVVALLGSVMTMPGLPKVP 537
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_ALISL (Formate--tetrahydrofolate ligase OS=Aliivibrio salmonicida (strain LFI1238) GN=fhs PE=3 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 1.521e-17
Identity = 42/103 (40.78%), Postives = 63/103 (61.17%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIA-RSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG+G  +L   V  AC   T+    LY  D+S+ +K+  +A + YGA  +E SE+A++Q+ M+   G+  L +CMA T  S S D   KGAPT FI+PIR+++
Sbjct:  411 GGEGTRELAQKVLIACAQETE-FTPLYTPDMSLLDKLTAVAIKGYGAERLELSEKAQQQLAMFEQLGYQHLSVCMAKTPASISTDGNIKGAPTDFIVPIRELR 512

HSP 2 Score: 36.965 bits (84), Expect = 1.521e-17
Identity = 15/29 (51.72%), Postives = 19/29 (65.52%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPCLL 425
            R   GAGF+Y L G + TMPGLP +P  +
Sbjct:  512 RLCAGAGFVYALCGNVMTMPGLPEKPAFM 540
The following BLAST results are available for this feature:
BLAST of EY679003 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 293
Match NameE-valueIdentityDescription
FTHS_SHELP5.320e-1844.66Formate--tetrahydrofolate ligase OS=Shewanella loi... [more]
FTHS_ALKOO5.322e-1839.81Formate--tetrahydrofolate ligase OS=Alkaliphilus o... [more]
FTHS_AERHH8.989e-1842.72Formate--tetrahydrofolate ligase OS=Aeromonas hydr... [more]
FTHS_GRABC8.990e-1842.72Formate--tetrahydrofolate ligase OS=Granulibacter ... [more]
FTHS_SHEAM8.994e-1842.86Formate--tetrahydrofolate ligase OS=Shewanella ama... [more]
FTHS_CLOD68.995e-1842.00Formate--tetrahydrofolate ligase OS=Clostridium di... [more]
FTHS_METPB8.995e-1839.81Formate--tetrahydrofolate ligase OS=Methylobacteri... [more]
FTHS_AERS41.519e-1743.69Formate--tetrahydrofolate ligase OS=Aeromonas salm... [more]
FTHS_FINM21.521e-1742.86Formate--tetrahydrofolate ligase OS=Finegoldia mag... [more]
FTHS_ALISL1.521e-1740.78Formate--tetrahydrofolate ligase OS=Aliivibrio sal... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-053-H07-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY679003 ID=EY679003; Name=EY679003; organism=Citrus sinensis; type=EST; length=876bp
GCCAGTTTAGTTTTCGTTCTCACCATCGCCCATGGGTGGAAAAGGAGCAG
TGGACCTTGGAATTGCAGTTCAAAGAGCCTGTGAGAATGTTACACAGCCA
TTGAAGTTCTTATATCCTTTAGATGTCAGTATTAAAGAGAAAATAGATAC
AATAGCAAGGTCATATGGTGCTAGCGGCGTTGAGTACTCAGAGGAGGCTG
AGAAACAGATCGAAATGTACACCGGGCAAGGGTTTTCTGGCTTACCAATT
TGCATGGCATAAACCCAGTATTCATTTTCACACGATGCCGCCGAGAAGGG
AGCCCCAACTGGGTTTATCTTACCGATAAGGGATGTAAAGAGCTAGCATT
GGGGCTGGATTCATTTATCCTTTGGTTGGAACAATGAGTACAATGCCAGG
TCTCCCAATAAGGCCATGCCTTTTAAGAGAATGGAGTCAACACTGCAACT
GGAAAGGTTGTTGGGGCTGTCTTAAGATCCCACAAAGCACTCCCGTGTGG
GTCCTCAAGAAGCTCTCCTTTAGATGTTCTTAAATGCTCCTGAGCAAACA
ATGAGCTTGAAGGGCTCCCCCCCCCCCCCACTGGCTGATATTCTAACCCC
GATCTGTAACACTTATGGGTACCTCGGGCTATTTGCTGTGGTAAACAAAA
CCCGACACCAAATAGAACCTGGAAAAAAAAAATCTTGGTACTCACCTTGC
GGACTTTAATTTTCCCTATTAATGTGTCCTTACTCCCATTTGATCTTGAA
TCCCGTTACGAAAATTTACCCTATGGGTGGGGGTGTGATATTTGTAAAAT
AAACAAAAAAAAAAAAAAAATAAAATAAAAGCAAAAGGGCGCGGGCGCGG
GTTAATAAATTATGAAAGGTATCAGA
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