EY679003

Overview
NameEY679003
Unique NameEY679003
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length876
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_VIBPA (Formate--tetrahydrofolate ligase OS=Vibrio parahaemolyticus GN=fhs PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 1.975e-17
Identity = 42/103 (40.78%), Postives = 63/103 (61.17%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG+G  DL   V  ACE  T   + LY    S++EK+ ++  + YGA+ VE SE A KQ+  +   GF+ L +C+A T  S + D++ KGAP GF +PIR+++
Sbjct:  439 GGEGTRDLAQYVVDACEKTTN-FRPLYQKHQSLQEKLMSVCEAGYGATNVEMSELATKQLAHFEKLGFNELAVCIAKTPLSVTTDSSVKGAPVGFTVPIRELR 540

HSP 2 Score: 36.965 bits (84), Expect = 1.975e-17
Identity = 15/29 (51.72%), Postives = 20/29 (68.97%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPCLL 425
            R   GAGF+Y L G++ TMPGLP +P  +
Sbjct:  540 RLCAGAGFVYALSGSVMTMPGLPDKPAFM 568
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_METEP (Formate--tetrahydrofolate ligase OS=Methylobacterium extorquens (strain PA1) GN=fhs PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 2.570e-17
Identity = 41/103 (39.81%), Postives = 60/103 (58.25%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIA-RSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG GA  L  AV +  E   +PL F Y  +  I +KI  IA + YGA+ ++   +A  ++  +   G+  LP+CMA TQYSFS D    GAP+G ++ +RDV+
Sbjct:  413 GGAGAEALAQAVVKLAEGEQKPLTFAYETETKITDKIKAIATKLYGAADIQIESKAATKLAGFEKDGYGKLPVCMAKTQYSFSTDPTLMGAPSGHLVSVRDVR 515

HSP 2 Score: 31.5722 bits (70), Expect = 2.570e-17
Identity = 14/26 (53.85%), Postives = 17/26 (65.38%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGF+  + G + TMPGLP  P
Sbjct:  515 RLSAGAGFVVVICGEIMTMPGLPKVP 540
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_METC4 (Formate--tetrahydrofolate ligase OS=Methylobacterium chloromethanicum (strain CM4 / NCIMB 13688) GN=fhs PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 2.570e-17
Identity = 41/103 (39.81%), Postives = 60/103 (58.25%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIA-RSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG GA  L  AV +  E   +PL F Y  +  I +KI  IA + YGA+ ++   +A  ++  +   G+  LP+CMA TQYSFS D    GAP+G ++ +RDV+
Sbjct:  413 GGAGAEALAQAVVKLAEGEQKPLTFAYETETKITDKIKAIATKLYGAADIQIESKAATKLAGFEKDGYGKLPVCMAKTQYSFSTDPTLMGAPSGHLVSVRDVR 515

HSP 2 Score: 31.5722 bits (70), Expect = 2.570e-17
Identity = 14/26 (53.85%), Postives = 17/26 (65.38%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGF+  + G + TMPGLP  P
Sbjct:  515 RLSAGAGFVVVICGEIMTMPGLPKVP 540
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_PHOPR (Formate--tetrahydrofolate ligase OS=Photobacterium profundum GN=fhs PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 4.338e-17
Identity = 40/103 (38.83%), Postives = 61/103 (59.22%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIAR-SYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG+GA +L  AV +ACE      K LY L  S++EK+  +A   YGA  +E S+ A++Q+      G   L +C+A T  S + D   KGAP+ F++P+R++K
Sbjct:  441 GGEGATELAQAVVKACETPAN-FKPLYSLSQSLEEKLMAVAEVGYGARSIELSDCAKEQLATLKKHGHDNLAVCLAKTPLSITTDPGIKGAPSDFVVPVRELK 542

HSP 2 Score: 35.8094 bits (81), Expect = 4.338e-17
Identity = 14/25 (56.00%), Postives = 18/25 (72.00%), Query Frame = 3 
Query:  351 GAGFIYPLVGTMSTMPGLPIRPCLL 425
            GAGF+Y L G + TMPGLP +P  +
Sbjct:  546 GAGFVYALCGNVMTMPGLPEKPAYM 570
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_STRMU (Formate--tetrahydrofolate ligase OS=Streptococcus mutans GN=fhs PE=3 SV=2)

HSP 1 Score: 74.7146 bits (182), Expect = 4.343e-17
Identity = 43/102 (42.16%), Postives = 58/102 (56.86%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDV 337
            G  G VDL   +    EN     K LY  ++S++EK+  IA+  Y A  V + ++A+ QI      G+  LPICMA TQYSFS D    GAPTGF + IR++
Sbjct:  412 GADGGVDLANTLINTIENNPSHYKRLYDNNLSVEEKVTEIAKEIYRADKVIFEKKAKTQIAQIVKNGWDNLPICMAKTQYSFSDDPKLLGAPTGFDITIREL 513

HSP 2 Score: 34.6538 bits (78), Expect = 4.343e-17
Identity = 15/26 (57.69%), Postives = 18/26 (69.23%), Query Frame = 3 
Query:  348 IGAGFIYPLVGTMSTMPGLPIRPCLL 425
            +GAGFI  L G + TMPGLP +P  L
Sbjct:  517 LGAGFIVALTGDVMTMPGLPKKPAAL 542
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_BACP2 (Formate--tetrahydrofolate ligase OS=Bacillus pumilus (strain SAFR-032) GN=fhs PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 5.644e-17
Identity = 40/103 (38.83%), Postives = 55/103 (53.40%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG+G   L   +    E       +LY  D SI+EK+  +A+  YGA GV  + +A KQ+       +  LP+CMA TQYS S D A  G P GF + IR++K
Sbjct:  416 GGEGGTALAEELTTLIEKKKNSFSYLYEEDDSIEEKLSKVAKVVYGADGVTLTSKARKQLAAIEENSWGHLPVCMAKTQYSLSDDPALIGRPKGFTITIRELK 518

HSP 2 Score: 37.3502 bits (85), Expect = 5.644e-17
Identity = 16/29 (55.17%), Postives = 21/29 (72.41%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPCLL 425
            + S+GAGFI  L G++ TMPGLP +P  L
Sbjct:  518 KPSVGAGFIVALTGSILTMPGLPKKPAAL 546
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_EUBE2 (Formate--tetrahydrofolate ligase OS=Eubacterium eligens (strain ATCC 27750 / VPI C15-48) GN=fhs PE=3 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 5.645e-17
Identity = 44/101 (43.56%), Postives = 54/101 (53.47%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRD 334
            GG+G ++L   V +  E        LY  D  +K KI+TIA+  YGA  V YS  A KQI      GF  LPICMA TQYS S D    G P GF + +R+
Sbjct:  412 GGEGGIELAEKVLKTLETKKSNFHCLYETDQPVKAKIETIAKEIYGAGSVNYSAAANKQIAQLEQLGFGNLPICMAKTQYSLSDDPTLLGRPEGFEMNVRE 512

HSP 2 Score: 30.0314 bits (66), Expect = 5.645e-17
Identity = 14/24 (58.33%), Postives = 16/24 (66.67%), Query Frame = 3 
Query:  345 SIGAGFIYPLVGTMSTMPGLPIRP 416
            S GAGFI  L G++ TMPGL   P
Sbjct:  516 SAGAGFIVVLTGSVMTMPGLSKSP 539
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_CLONN (Formate--tetrahydrofolate ligase OS=Clostridium novyi (strain NT) GN=fhs PE=3 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 5.645e-17
Identity = 45/103 (43.69%), Postives = 59/103 (57.28%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG+G + L   V    +      K LY   +SIKEK+D IAR  YGASGV+Y + AEK I+     G   LPIC+A TQYS S + +  G P  F + +R+VK
Sbjct:  412 GGEGGIALAEKVINVLDTQKSNFKCLYDEKLSIKEKMDIIAREIYGASGVDYDKSAEKDIKDIEKLGLDKLPICVAKTQYSLSDNPSLLGKPEDFRVNVREVK 514

HSP 2 Score: 31.9574 bits (71), Expect = 5.645e-17
Identity = 15/26 (57.69%), Postives = 17/26 (65.38%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            + S GAGFI  L G + TMPGLP  P
Sbjct:  514 KVSNGAGFIVVLTGNIMTMPGLPKVP 539
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_STAES (Formate--tetrahydrofolate ligase OS=Staphylococcus epidermidis (strain ATCC 12228) GN=fhs PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 5.645e-17
Identity = 41/104 (39.42%), Postives = 61/104 (58.65%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            GG+G ++L   V    +   Q  K LY L   ++ KI+TI +  YG S V +S +A+KQ++ +   G+   PICMA TQYSFS D    GAP  F + IR++++
Sbjct:  412 GGEGGIELANQVFDVMQE-PQNFKHLYELKQPLEAKIETIVKEIYGGSKVNFSSKAQKQLKQFKENGWDEYPICMAKTQYSFSDDQTLLGAPNDFEITIRELEA 514

HSP 2 Score: 35.4242 bits (80), Expect = 5.645e-17
Identity = 16/28 (57.14%), Postives = 18/28 (64.29%), Query Frame = 3 
Query:  342 ASIGAGFIYPLVGTMSTMPGLPIRPCLL 425
            A  GAGFI  L G + TMPGLP +P  L
Sbjct:  514 AKTGAGFIVALTGAIMTMPGLPKKPAAL 541
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_STAEQ (Formate--tetrahydrofolate ligase OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=fhs PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 5.645e-17
Identity = 41/104 (39.42%), Postives = 61/104 (58.65%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            GG+G ++L   V    +   Q  K LY L   ++ KI+TI +  YG S V +S +A+KQ++ +   G+   PICMA TQYSFS D    GAP  F + IR++++
Sbjct:  412 GGEGGIELANQVFDVMQE-PQNFKHLYELKQPLEAKIETIVKEIYGGSKVNFSSKAQKQLKQFKENGWDEYPICMAKTQYSFSDDQTLLGAPNDFEITIRELEA 514

HSP 2 Score: 35.4242 bits (80), Expect = 5.645e-17
Identity = 16/28 (57.14%), Postives = 18/28 (64.29%), Query Frame = 3 
Query:  342 ASIGAGFIYPLVGTMSTMPGLPIRPCLL 425
            A  GAGFI  L G + TMPGLP +P  L
Sbjct:  514 AKTGAGFIVALTGAIMTMPGLPKKPAAL 541
The following BLAST results are available for this feature:
BLAST of EY679003 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 293
Match NameE-valueIdentityDescription
FTHS_VIBPA1.975e-1740.78Formate--tetrahydrofolate ligase OS=Vibrio parahae... [more]
FTHS_METEP2.570e-1739.81Formate--tetrahydrofolate ligase OS=Methylobacteri... [more]
FTHS_METC42.570e-1739.81Formate--tetrahydrofolate ligase OS=Methylobacteri... [more]
FTHS_PHOPR4.338e-1738.83Formate--tetrahydrofolate ligase OS=Photobacterium... [more]
FTHS_STRMU4.343e-1742.16Formate--tetrahydrofolate ligase OS=Streptococcus ... [more]
FTHS_BACP25.644e-1738.83Formate--tetrahydrofolate ligase OS=Bacillus pumil... [more]
FTHS_EUBE25.645e-1743.56Formate--tetrahydrofolate ligase OS=Eubacterium el... [more]
FTHS_CLONN5.645e-1743.69Formate--tetrahydrofolate ligase OS=Clostridium no... [more]
FTHS_STAES5.645e-1739.42Formate--tetrahydrofolate ligase OS=Staphylococcus... [more]
FTHS_STAEQ5.645e-1739.42Formate--tetrahydrofolate ligase OS=Staphylococcus... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-053-H07-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY679003 ID=EY679003; Name=EY679003; organism=Citrus sinensis; type=EST; length=876bp
GCCAGTTTAGTTTTCGTTCTCACCATCGCCCATGGGTGGAAAAGGAGCAG
TGGACCTTGGAATTGCAGTTCAAAGAGCCTGTGAGAATGTTACACAGCCA
TTGAAGTTCTTATATCCTTTAGATGTCAGTATTAAAGAGAAAATAGATAC
AATAGCAAGGTCATATGGTGCTAGCGGCGTTGAGTACTCAGAGGAGGCTG
AGAAACAGATCGAAATGTACACCGGGCAAGGGTTTTCTGGCTTACCAATT
TGCATGGCATAAACCCAGTATTCATTTTCACACGATGCCGCCGAGAAGGG
AGCCCCAACTGGGTTTATCTTACCGATAAGGGATGTAAAGAGCTAGCATT
GGGGCTGGATTCATTTATCCTTTGGTTGGAACAATGAGTACAATGCCAGG
TCTCCCAATAAGGCCATGCCTTTTAAGAGAATGGAGTCAACACTGCAACT
GGAAAGGTTGTTGGGGCTGTCTTAAGATCCCACAAAGCACTCCCGTGTGG
GTCCTCAAGAAGCTCTCCTTTAGATGTTCTTAAATGCTCCTGAGCAAACA
ATGAGCTTGAAGGGCTCCCCCCCCCCCCCACTGGCTGATATTCTAACCCC
GATCTGTAACACTTATGGGTACCTCGGGCTATTTGCTGTGGTAAACAAAA
CCCGACACCAAATAGAACCTGGAAAAAAAAAATCTTGGTACTCACCTTGC
GGACTTTAATTTTCCCTATTAATGTGTCCTTACTCCCATTTGATCTTGAA
TCCCGTTACGAAAATTTACCCTATGGGTGGGGGTGTGATATTTGTAAAAT
AAACAAAAAAAAAAAAAAAATAAAATAAAAGCAAAAGGGCGCGGGCGCGG
GTTAATAAATTATGAAAGGTATCAGA
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