EY679003

Overview
NameEY679003
Unique NameEY679003
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length876
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_LACS1 (Formate--tetrahydrofolate ligase OS=Lactobacillus salivarius subsp. salivarius (strain UCC118) GN=fhs PE=3 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 5.646e-17
Identity = 44/101 (43.56%), Postives = 64/101 (63.37%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRD 334
            GG+GA +L  AV  +C+N  Q    LY  D S++EKI+ I    YG  GVE+S +A++Q+  +   G++ LP+CMA TQYS S +A   GAP GF + +R+
Sbjct:  409 GGEGATELAKAVIESCDN-DQEFTRLYNSDDSVEEKINKIVTEIYGGDGVEFSAKAKRQLRQFEKLGWNHLPVCMAKTQYSLSDNAKVLGAPKGFKIHVRE 508

HSP 2 Score: 27.7202 bits (60), Expect = 5.646e-17
Identity = 12/23 (52.17%), Postives = 15/23 (65.22%), Query Frame = 3 
Query:  348 IGAGFIYPLVGTMSTMPGLPIRP 416
            +GA F+  L G + TMPGLP  P
Sbjct:  513 LGAQFLVALTGNILTMPGLPKVP 535
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_VIBSL (Formate--tetrahydrofolate ligase OS=Vibrio splendidus (strain LGP32) GN=fhs PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 9.529e-17
Identity = 40/103 (38.83%), Postives = 64/103 (62.14%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIAR-SYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG+GA  L  AV +AC+  ++  K LY  + S++EK+  +A   YGA+ +  S  A+KQ+  +   G+S L +C+A T  S S +A  KGAP+ F +P+R++K
Sbjct:  439 GGEGATQLAHAVVKACQKQSE-FKPLYHSEQSLEEKLMAVAEVGYGAASISLSPLAKKQLAEFKLHGYSDLSVCLAKTPLSISTEAHIKGAPSQFDVPVRELK 540

HSP 2 Score: 36.5798 bits (83), Expect = 9.529e-17
Identity = 15/25 (60.00%), Postives = 18/25 (72.00%), Query Frame = 3 
Query:  351 GAGFIYPLVGTMSTMPGLPIRPCLL 425
            GAGFIY L G + TMPGLP +P  +
Sbjct:  544 GAGFIYALCGNVMTMPGLPDKPAFM 568
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS2_DESHY (Formate--tetrahydrofolate ligase 2 OS=Desulfitobacterium hafniense (strain Y51) GN=fhs2 PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 9.538e-17
Identity = 43/103 (41.75%), Postives = 60/103 (58.25%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIA-RSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG G V+L   V +  ++  +   F Y +D+ +KEKI  IA + YGA GV++   + K IE     G+  +P+CMA TQYS S D  + G PTGF + IR VK
Sbjct:  415 GGAGGVELAEEVLKLMDS-PKNFTFAYDIDLGLKEKITAIATKIYGADGVDFIGSSTKDIEGIESIGYRNIPVCMAKTQYSLSDDQKKLGRPTGFRISIRSVK 516

HSP 2 Score: 30.8018 bits (68), Expect = 9.538e-17
Identity = 14/26 (53.85%), Postives = 16/26 (61.54%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            + S GAGF   L G + TMPGLP  P
Sbjct:  516 KVSAGAGFAVALTGDIMTMPGLPKVP 541
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_METNO (Formate--tetrahydrofolate ligase OS=Methylobacterium nodulans (strain ORS2060 / LMG 21967) GN=fhs PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 9.538e-17
Identity = 41/104 (39.42%), Postives = 62/104 (59.62%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACEN-VTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG GA +L  AV    E    +   F+YP D  + +KI TIA++ YGA+ ++   +A  ++  +   G+  LP+CMA TQYSFS D    GAP+G ++ +RDV+
Sbjct:  413 GGAGAEELARAVVALAEGGAPKTPNFVYPEDAKLTDKIRTIAQTLYGAADIQVESKAATKLAQFEKDGYGKLPVCMAKTQYSFSTDPNLMGAPSGHVVAVRDVR 516

HSP 2 Score: 31.5722 bits (70), Expect = 9.538e-17
Identity = 14/26 (53.85%), Postives = 17/26 (65.38%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGF+  + G + TMPGLP  P
Sbjct:  516 RLSAGAGFVVVICGEIMTMPGLPKVP 541
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_METS4 (Formate--tetrahydrofolate ligase OS=Methylobacterium sp. (strain 4-46) GN=fhs PE=3 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.611e-16
Identity = 41/104 (39.42%), Postives = 61/104 (58.65%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPL-KFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG GA DL  AV    E        F+YP +  + +KI TIA++ YGA+ ++   +A  ++  +   G+  LP+CMA TQYSFS D    GAP+G ++ +RDV+
Sbjct:  413 GGAGAEDLARAVVSLAEGGAPATPNFVYPEEAKLTDKIRTIAQTLYGAADIQVESKAAAKLAQFEKDGYGRLPVCMAKTQYSFSTDPGLIGAPSGHVVAVRDVR 516

HSP 2 Score: 31.5722 bits (70), Expect = 1.611e-16
Identity = 14/26 (53.85%), Postives = 17/26 (65.38%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGF+  + G + TMPGLP  P
Sbjct:  516 RLSAGAGFVVVICGEIMTMPGLPKVP 541
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_BEII9 (Formate--tetrahydrofolate ligase OS=Beijerinckia indica subsp. indica (strain ATCC 9039 / DSM 1715 / NCIB 8712) GN=fhs PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 1.612e-16
Identity = 44/103 (42.72%), Postives = 60/103 (58.25%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GGKGA+ L   V    E  T   K LY   + + EK+  IA+  YGA+ +    +A+KQ+     QGF   P+C+A TQYSF+ D    GAPTG I+PIR+V+
Sbjct:  411 GGKGALALAEKVIALVEGGTAQFKMLYEDTLPLIEKMRRIAQEIYGAADISLDAKAKKQLADIEAQGFGHFPVCVAKTQYSFAADPKLLGAPTGHIVPIREVR 513

HSP 2 Score: 30.0314 bits (66), Expect = 1.612e-16
Identity = 13/26 (50.00%), Postives = 17/26 (65.38%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGF+  + G + TMPGL  +P
Sbjct:  513 RLSAGAGFVVMICGDIMTMPGLSRQP 538
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_CLOBB (Formate--tetrahydrofolate ligase OS=Clostridium botulinum (strain Eklund 17B / Type B) GN=fhs PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.612e-16
Identity = 45/103 (43.69%), Postives = 59/103 (57.28%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG G ++LG AV    E      K LY  D +I+EKI TIA+  YGA GV YS EA+KQI          LPICMA TQYS S +      P+GF + +++++
Sbjct:  412 GGDGGIELGEAVLDVIEKDKSNFKTLYDTDKTIEEKILTIAKEIYGADGVVYSNEAKKQIGELVKFNLDKLPICMAKTQYSLSDNPNLLAKPSGFNINVQEIR 514

HSP 2 Score: 30.8018 bits (68), Expect = 1.612e-16
Identity = 15/26 (57.69%), Postives = 16/26 (61.54%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGFI    G + TMPGLP  P
Sbjct:  514 RVSNGAGFIVVQTGNIMTMPGLPKVP 539
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_CLOBA (Formate--tetrahydrofolate ligase OS=Clostridium botulinum (strain Alaska E43 / Type E3) GN=fhs PE=3 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.612e-16
Identity = 45/103 (43.69%), Postives = 59/103 (57.28%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG G ++LG AV    E      K LY  D +I+EKI TIA+  YGA GV YS EA+KQI          LPICMA TQYS S +      P+GF + +++++
Sbjct:  412 GGDGGIELGEAVLDVIEKDKSSFKTLYKADNTIEEKILTIAKEIYGADGVVYSNEAKKQIGELVKFNLDKLPICMAKTQYSLSDNPNLLAKPSGFNINVQEIR 514

HSP 2 Score: 30.8018 bits (68), Expect = 1.612e-16
Identity = 15/26 (57.69%), Postives = 16/26 (61.54%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGFI    G + TMPGLP  P
Sbjct:  514 RVSNGAGFIVVQTGNIMTMPGLPKVP 539
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_FUSNN (Formate--tetrahydrofolate ligase OS=Fusobacterium nucleatum subsp. nucleatum GN=fhs PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 1.612e-16
Identity = 38/103 (36.89%), Postives = 62/103 (60.19%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG+G +DL   V +A +N      + Y ++++IKEKI+ I +  YGA GV ++   +K  ++   +G + LP+CM+ TQ S S + A  G PTGF + I D++
Sbjct:  400 GGEGGIDLAEKVLKAIDNNKTEFDYFYDINLTIKEKIEKICKEIYGADGVIFAPATKKVFDVIEAEGLNKLPVCMSKTQKSISDNPALLGKPTGFKVTINDLR 502

HSP 2 Score: 31.9574 bits (71), Expect = 1.612e-16
Identity = 12/26 (46.15%), Postives = 18/26 (69.23%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R ++GAGF+  + G +  MPGLP +P
Sbjct:  502 RLAVGAGFVIAMAGDIIDMPGLPKKP 527
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_SHESH (Formate--tetrahydrofolate ligase OS=Shewanella sediminis (strain HAW-EB3) GN=fhs PE=3 SV=1)

HSP 1 Score: 73.559 bits (179), Expect = 2.094e-16
Identity = 44/103 (42.72%), Postives = 59/103 (57.28%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            G KGA  L   V  A E  +   KFLY LD SI+ K+ TIA + YGA+G++ S +A+ Q++     G   L +C+A T  S SH+   KG PT F LPI  +K
Sbjct:  427 GAKGAEALARVVVSATEQKSD-FKFLYQLDESIESKLLTIAEAGYGAAGIKLSPDAKIQLQEINSMGLDKLAVCVAKTPLSISHEPKVKGVPTDFELPITQLK 528

HSP 2 Score: 33.4982 bits (75), Expect = 2.094e-16
Identity = 15/26 (57.69%), Postives = 19/26 (73.08%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            + + GAGFI  LVG + TMPGL I+P
Sbjct:  528 KINAGAGFITALVGKVMTMPGLGIKP 553
The following BLAST results are available for this feature:
BLAST of EY679003 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 293
Match NameE-valueIdentityDescription
FTHS_LACS15.646e-1743.56Formate--tetrahydrofolate ligase OS=Lactobacillus ... [more]
FTHS_VIBSL9.529e-1738.83Formate--tetrahydrofolate ligase OS=Vibrio splendi... [more]
FTHS2_DESHY9.538e-1741.75Formate--tetrahydrofolate ligase 2 OS=Desulfitobac... [more]
FTHS_METNO9.538e-1739.42Formate--tetrahydrofolate ligase OS=Methylobacteri... [more]
FTHS_METS41.611e-1639.42Formate--tetrahydrofolate ligase OS=Methylobacteri... [more]
FTHS_BEII91.612e-1642.72Formate--tetrahydrofolate ligase OS=Beijerinckia i... [more]
FTHS_CLOBB1.612e-1643.69Formate--tetrahydrofolate ligase OS=Clostridium bo... [more]
FTHS_CLOBA1.612e-1643.69Formate--tetrahydrofolate ligase OS=Clostridium bo... [more]
FTHS_FUSNN1.612e-1636.89Formate--tetrahydrofolate ligase OS=Fusobacterium ... [more]
FTHS_SHESH2.094e-1642.72Formate--tetrahydrofolate ligase OS=Shewanella sed... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-053-H07-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY679003 ID=EY679003; Name=EY679003; organism=Citrus sinensis; type=EST; length=876bp
GCCAGTTTAGTTTTCGTTCTCACCATCGCCCATGGGTGGAAAAGGAGCAG
TGGACCTTGGAATTGCAGTTCAAAGAGCCTGTGAGAATGTTACACAGCCA
TTGAAGTTCTTATATCCTTTAGATGTCAGTATTAAAGAGAAAATAGATAC
AATAGCAAGGTCATATGGTGCTAGCGGCGTTGAGTACTCAGAGGAGGCTG
AGAAACAGATCGAAATGTACACCGGGCAAGGGTTTTCTGGCTTACCAATT
TGCATGGCATAAACCCAGTATTCATTTTCACACGATGCCGCCGAGAAGGG
AGCCCCAACTGGGTTTATCTTACCGATAAGGGATGTAAAGAGCTAGCATT
GGGGCTGGATTCATTTATCCTTTGGTTGGAACAATGAGTACAATGCCAGG
TCTCCCAATAAGGCCATGCCTTTTAAGAGAATGGAGTCAACACTGCAACT
GGAAAGGTTGTTGGGGCTGTCTTAAGATCCCACAAAGCACTCCCGTGTGG
GTCCTCAAGAAGCTCTCCTTTAGATGTTCTTAAATGCTCCTGAGCAAACA
ATGAGCTTGAAGGGCTCCCCCCCCCCCCCACTGGCTGATATTCTAACCCC
GATCTGTAACACTTATGGGTACCTCGGGCTATTTGCTGTGGTAAACAAAA
CCCGACACCAAATAGAACCTGGAAAAAAAAAATCTTGGTACTCACCTTGC
GGACTTTAATTTTCCCTATTAATGTGTCCTTACTCCCATTTGATCTTGAA
TCCCGTTACGAAAATTTACCCTATGGGTGGGGGTGTGATATTTGTAAAAT
AAACAAAAAAAAAAAAAAAATAAAATAAAAGCAAAAGGGCGCGGGCGCGG
GTTAATAAATTATGAAAGGTATCAGA
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