EY679003

Overview
NameEY679003
Unique NameEY679003
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length876
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_DEHE1 (Formate--tetrahydrofolate ligase OS=Dehalococcoides ethenogenes (strain 195) GN=fhs1 PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 4.592e-16
Identity = 48/103 (46.60%), Postives = 62/103 (60.19%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSG-LPICMA*TQYSFSHDAAEKGAPTGFILPIRDV 337
            GG+GA +L  AV  AC N      FLYP D+ ++E+I TIAR  YGA GV Y++ A +++      G +  +P CM  T  S SHD A KG P GF LPIRD+
Sbjct:  452 GGEGAAELAEAVIDAC-NEPNDFHFLYPEDMPLRERIYTIARKVYGADGVSYTQTALEKLARLENTGNTQFMPSCMVKTHLSLSHDPALKGRPGGFTLPIRDI 553

HSP 2 Score: 24.2534 bits (51), Expect = 4.592e-16
Identity = 9/23 (39.13%), Postives = 13/23 (56.52%), Query Frame = 3 
Query:  348 IGAGFIYPLVGTMSTMPGLPIRP 416
            +GAG + P+ G +  MPG    P
Sbjct:  557 MGAGLVVPVAGDIKLMPGTSSDP 579
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_STACT (Formate--tetrahydrofolate ligase OS=Staphylococcus carnosus (strain TM300) GN=fhs PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 4.599e-16
Identity = 40/103 (38.83%), Postives = 58/103 (56.31%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GGKG V+L   VQ    N     K  Y LD+ I+EKI+ +  + YG + V ++  A KQ++     G+   P+CMA TQYSF+ D    GAP  F + IR+++
Sbjct:  412 GGKGGVELAKQVQEVL-NEKHDFKHTYDLDLPIEEKIEKVVTNIYGGNKVTFTSGALKQLKQIKENGWDNYPVCMAKTQYSFTDDKDRLGAPDDFEITIRELQ 513

HSP 2 Score: 34.2686 bits (77), Expect = 4.599e-16
Identity = 15/25 (60.00%), Postives = 17/25 (68.00%), Query Frame = 3 
Query:  351 GAGFIYPLVGTMSTMPGLPIRPCLL 425
            GAGFI  L G + TMPGLP +P  L
Sbjct:  517 GAGFIVALTGAIMTMPGLPKKPAAL 541
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_NATTJ (Formate--tetrahydrofolate ligase OS=Natranaerobius thermophilus (strain ATCC BAA-1301 / DSM 18059 / JW/NM-WN-LF) GN=fhs PE=3 SV=1)

HSP 1 Score: 73.1738 bits (178), Expect = 5.978e-16
Identity = 38/103 (36.89%), Postives = 61/103 (59.22%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG+G  ++   V    ++     K+LY +D S++EKI+TIA+  YGAS VE++  A + I+    +G   +P+CMA TQ+SFS D    G P  F + ++ V+
Sbjct:  412 GGEGGEEVAKEVLDIIDSKESNFKYLYDVDKSMEEKIETIAKEIYGASDVEFTPTARRNIKQLAQKGLDQVPVCMAKTQFSFSDDPKLLGRPKDFSITVKRVR 514

HSP 2 Score: 32.3426 bits (72), Expect = 5.978e-16
Identity = 14/26 (53.85%), Postives = 17/26 (65.38%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGF   + G + TMPGLP +P
Sbjct:  514 RISAGAGFAVAMTGDIMTMPGLPKQP 539
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_LACRJ (Formate--tetrahydrofolate ligase OS=Lactobacillus reuteri (strain JCM 1112) GN=fhs PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 5.978e-16
Identity = 39/105 (37.14%), Postives = 61/105 (58.10%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKE---KIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDV 337
            GGKG  DL     +  E    P +F +  DV + +   K++ IA+  YGA  V +S +A+ Q++ +   G++ LP+C+A TQYSF+ D  + GAPT F   IR++
Sbjct:  409 GGKGTHDLA---NKVVELADSPSEFTHIYDVQVDDLQTKLEKIAKQIYGAKEVSFSRKAQNQLKRFAKYGWNDLPVCIAKTQYSFTDDQKQLGAPTDFTFHIREL 510

HSP 2 Score: 35.039 bits (79), Expect = 5.978e-16
Identity = 15/23 (65.22%), Postives = 16/23 (69.57%), Query Frame = 3 
Query:  348 IGAGFIYPLVGTMSTMPGLPIRP 416
            IGAGF+  L G M TMPGLP  P
Sbjct:  514 IGAGFVVALAGNMMTMPGLPKEP 536
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_LACRD (Formate--tetrahydrofolate ligase OS=Lactobacillus reuteri (strain DSM 20016) GN=fhs PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 5.978e-16
Identity = 39/105 (37.14%), Postives = 61/105 (58.10%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKE---KIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDV 337
            GGKG  DL     +  E    P +F +  DV + +   K++ IA+  YGA  V +S +A+ Q++ +   G++ LP+C+A TQYSF+ D  + GAPT F   IR++
Sbjct:  409 GGKGTHDLA---NKVVELADSPSEFTHIYDVQVDDLQTKLEKIAKQIYGAKEVSFSRKAQNQLKRFAKYGWNDLPVCIAKTQYSFTDDQKQLGAPTDFTFHIREL 510

HSP 2 Score: 35.039 bits (79), Expect = 5.978e-16
Identity = 15/23 (65.22%), Postives = 16/23 (69.57%), Query Frame = 3 
Query:  348 IGAGFIYPLVGTMSTMPGLPIRP 416
            IGAGF+  L G M TMPGLP  P
Sbjct:  514 IGAGFVVALAGNMMTMPGLPKEP 536
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_SYNFM (Formate--tetrahydrofolate ligase OS=Syntrophobacter fumaroxidans (strain DSM 10017 / MPOB) GN=fhs PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 7.758e-16
Identity = 41/103 (39.81%), Postives = 64/103 (62.14%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQG-FSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDV 337
            GG+GA++   AV  AC+  +Q  KFLYPL++ +++++ T+AR  YGA GV ++ +AE + +M      +      M  T  S +HD + KG P G++LPIRDV
Sbjct:  442 GGEGALEFADAVIDACKQESQ-FKFLYPLEMKLRDRVSTVAREVYGADGVSWTPDAEAKAKMLENDPKYDDYATMMVKTHLSLTHDPSVKGVPKGWVLPIRDV 543

HSP 2 Score: 27.335 bits (59), Expect = 7.758e-16
Identity = 12/26 (46.15%), Postives = 14/26 (53.85%), Query Frame = 3 
Query:  351 GAGFIYPLVGTMSTMPGLPIRPCLLR 428
            GA F+ P  GT+S MPG    P   R
Sbjct:  548 GAKFLCPCAGTISLMPGTSSNPAFRR 573
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_SHEHH (Formate--tetrahydrofolate ligase OS=Shewanella halifaxensis (strain HAW-EB4) GN=fhs PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 7.764e-16
Identity = 41/103 (39.81%), Postives = 58/103 (56.31%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIAR-SYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            G +GA  L   V  A +  +   K LY  D S++ K+ T+A   YGAS V  S++A+ Q+   T   +  LPIC+A T  S SHD + KG PT F LPI +++
Sbjct:  427 GAEGATKLADMVYHATQTPSD-FKLLYQSDTSLEAKLMTLAEVGYGASSVTLSDKAKSQLHWLTKHNYGQLPICVAKTPMSISHDPSIKGIPTEFELPITELR 528

HSP 2 Score: 34.2686 bits (77), Expect = 7.764e-16
Identity = 15/26 (57.69%), Postives = 19/26 (73.08%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R + GAGF+  LVG + TMPGL I+P
Sbjct:  528 RLNAGAGFVTALVGQVMTMPGLGIKP 553
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_LISMH (Formate--tetrahydrofolate ligase OS=Listeria monocytogenes serotype 4a (strain HCC23) GN=fhs PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 7.767e-16
Identity = 42/101 (41.58%), Postives = 57/101 (56.44%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIA-RSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRD 334
            GG G ++L   V  A E+     K +Y    SI+EK++ I  + YG  GVE S +A+KQI  +   G+   PICMA TQYS S D    G PT F++ IR+
Sbjct:  416 GGDGGLELADKVIAAVESGEADYKRIYDDAWSIEEKLEAIVTKVYGGIGVELSSKAQKQIVEFKKYGWDRYPICMAKTQYSLSDDPTLLGRPTDFVIHIRE 516

HSP 2 Score: 34.2686 bits (77), Expect = 7.767e-16
Identity = 14/26 (53.85%), Postives = 18/26 (69.23%), Query Frame = 3 
Query:  348 IGAGFIYPLVGTMSTMPGLPIRPCLL 425
            +GAGF+  L G + TMPGLP +P  L
Sbjct:  521 LGAGFVVALTGDVMTMPGLPKKPAAL 546
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_LISMF (Formate--tetrahydrofolate ligase OS=Listeria monocytogenes serotype 4b (strain F2365) GN=fhs PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 7.767e-16
Identity = 42/101 (41.58%), Postives = 57/101 (56.44%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIA-RSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRD 334
            GG G ++L   V  A E+     K +Y    SI+EK++ I  + YG  GVE S +A+KQI  +   G+   PICMA TQYS S D    G PT F++ IR+
Sbjct:  416 GGDGGLELADKVIAAVESGEADYKRIYDDAWSIEEKLEAIVTKVYGGIGVELSSKAQKQIVEFKKYGWDRYPICMAKTQYSLSDDPTLLGRPTDFVIHIRE 516

HSP 2 Score: 34.2686 bits (77), Expect = 7.767e-16
Identity = 14/26 (53.85%), Postives = 18/26 (69.23%), Query Frame = 3 
Query:  348 IGAGFIYPLVGTMSTMPGLPIRPCLL 425
            +GAGF+  L G + TMPGLP +P  L
Sbjct:  521 LGAGFVVALTGDVMTMPGLPKKPAAL 546
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_LISMC (Formate--tetrahydrofolate ligase OS=Listeria monocytogenes serotype 4b (strain Clip81459) GN=fhs PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 7.767e-16
Identity = 42/101 (41.58%), Postives = 57/101 (56.44%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIA-RSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRD 334
            GG G ++L   V  A E+     K +Y    SI+EK++ I  + YG  GVE S +A+KQI  +   G+   PICMA TQYS S D    G PT F++ IR+
Sbjct:  416 GGDGGLELADKVIAAVESGEADYKRIYDDAWSIEEKLEAIVTKVYGGIGVELSSKAQKQIVEFKKYGWDRYPICMAKTQYSLSDDPTLLGRPTDFVIHIRE 516

HSP 2 Score: 34.2686 bits (77), Expect = 7.767e-16
Identity = 14/26 (53.85%), Postives = 18/26 (69.23%), Query Frame = 3 
Query:  348 IGAGFIYPLVGTMSTMPGLPIRPCLL 425
            +GAGF+  L G + TMPGLP +P  L
Sbjct:  521 LGAGFVVALTGDVMTMPGLPKKPAAL 546
The following BLAST results are available for this feature:
BLAST of EY679003 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 293
Match NameE-valueIdentityDescription
FTHS_DEHE14.592e-1646.60Formate--tetrahydrofolate ligase OS=Dehalococcoide... [more]
FTHS_STACT4.599e-1638.83Formate--tetrahydrofolate ligase OS=Staphylococcus... [more]
FTHS_NATTJ5.978e-1636.89Formate--tetrahydrofolate ligase OS=Natranaerobius... [more]
FTHS_LACRJ5.978e-1637.14Formate--tetrahydrofolate ligase OS=Lactobacillus ... [more]
FTHS_LACRD5.978e-1637.14Formate--tetrahydrofolate ligase OS=Lactobacillus ... [more]
FTHS_SYNFM7.758e-1639.81Formate--tetrahydrofolate ligase OS=Syntrophobacte... [more]
FTHS_SHEHH7.764e-1639.81Formate--tetrahydrofolate ligase OS=Shewanella hal... [more]
FTHS_LISMH7.767e-1641.58Formate--tetrahydrofolate ligase OS=Listeria monoc... [more]
FTHS_LISMF7.767e-1641.58Formate--tetrahydrofolate ligase OS=Listeria monoc... [more]
FTHS_LISMC7.767e-1641.58Formate--tetrahydrofolate ligase OS=Listeria monoc... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-053-H07-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY679003 ID=EY679003; Name=EY679003; organism=Citrus sinensis; type=EST; length=876bp
GCCAGTTTAGTTTTCGTTCTCACCATCGCCCATGGGTGGAAAAGGAGCAG
TGGACCTTGGAATTGCAGTTCAAAGAGCCTGTGAGAATGTTACACAGCCA
TTGAAGTTCTTATATCCTTTAGATGTCAGTATTAAAGAGAAAATAGATAC
AATAGCAAGGTCATATGGTGCTAGCGGCGTTGAGTACTCAGAGGAGGCTG
AGAAACAGATCGAAATGTACACCGGGCAAGGGTTTTCTGGCTTACCAATT
TGCATGGCATAAACCCAGTATTCATTTTCACACGATGCCGCCGAGAAGGG
AGCCCCAACTGGGTTTATCTTACCGATAAGGGATGTAAAGAGCTAGCATT
GGGGCTGGATTCATTTATCCTTTGGTTGGAACAATGAGTACAATGCCAGG
TCTCCCAATAAGGCCATGCCTTTTAAGAGAATGGAGTCAACACTGCAACT
GGAAAGGTTGTTGGGGCTGTCTTAAGATCCCACAAAGCACTCCCGTGTGG
GTCCTCAAGAAGCTCTCCTTTAGATGTTCTTAAATGCTCCTGAGCAAACA
ATGAGCTTGAAGGGCTCCCCCCCCCCCCCACTGGCTGATATTCTAACCCC
GATCTGTAACACTTATGGGTACCTCGGGCTATTTGCTGTGGTAAACAAAA
CCCGACACCAAATAGAACCTGGAAAAAAAAAATCTTGGTACTCACCTTGC
GGACTTTAATTTTCCCTATTAATGTGTCCTTACTCCCATTTGATCTTGAA
TCCCGTTACGAAAATTTACCCTATGGGTGGGGGTGTGATATTTGTAAAAT
AAACAAAAAAAAAAAAAAAATAAAATAAAAGCAAAAGGGCGCGGGCGCGG
GTTAATAAATTATGAAAGGTATCAGA
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