EY679003

Overview
NameEY679003
Unique NameEY679003
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length876
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_ARTAT (Formate--tetrahydrofolate ligase OS=Arthrobacter aurescens (strain TC1) GN=fhs PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 2.961e-19
Identity = 45/102 (44.12%), Postives = 64/102 (62.75%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDV 337
            GG G  +L   V  A E  +     LYPL++ +++KI TI +  YGA GVE+S  A K++      G+SGLP+CMA TQYSF+ DA+  GAP GF + +R++
Sbjct:  419 GGDGGDELAAKVAAALEAPSN-FHHLYPLELGVEDKIRTIVQEIYGADGVEFSVPALKRLAEIEKNGWSGLPVCMAKTQYSFTDDASRLGAPKGFRVHVREL 519

HSP 2 Score: 34.6538 bits (78), Expect = 2.961e-19
Identity = 15/26 (57.69%), Postives = 17/26 (65.38%), Query Frame = 3 
Query:  351 GAGFIYPLVGTMSTMPGLPIRPCLLR 428
            GAGFI  L G + TMPGLP  P  +R
Sbjct:  524 GAGFIVALTGAVMTMPGLPKEPAAMR 549
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_BACLD (Formate--tetrahydrofolate ligase OS=Bacillus licheniformis (strain DSM 13 / ATCC 14580) GN=fhs PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 2.962e-19
Identity = 43/103 (41.75%), Postives = 61/103 (59.22%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GGKG  DL   V    E       +LY L  SI++K+  I+R+ YGA GVE++E+A+KQ+      G  GLP+C+A TQYS S D  + G P  F + +R++K
Sbjct:  415 GGKGGTDLAREVIHVMEQKDNRFSYLYELTDSIEDKLAKISRTVYGAEGVEFTEKAKKQLLELKKNGLDGLPVCVAKTQYSLSDDPGKIGRPQDFSITVRELK 517

HSP 2 Score: 35.039 bits (79), Expect = 2.962e-19
Identity = 16/30 (53.33%), Postives = 20/30 (66.67%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPCLLR 428
            + S GAGFI  L G++ TMPGLP  P  L+
Sbjct:  517 KPSRGAGFIVALTGSILTMPGLPKHPAALK 546
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_SYNWW (Formate--tetrahydrofolate ligase OS=Syntrophomonas wolfei subsp. wolfei (strain Goettingen) GN=fhs PE=3 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 2.962e-19
Identity = 48/103 (46.60%), Postives = 65/103 (63.11%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG+G +DL   +  A E  +Q  KFLY L+  +K KI+TI R  YGA  V+Y+ EA++ ++ Y   GF  LP+C+A TQYS S +A   G PTGF + IR VK
Sbjct:  412 GGEGGIDLAEKLVLATEQGSQ-FKFLYELNQPLKNKIETICREIYGAGSVKYAAEAKRMLQKYEESGFGDLPVCIAKTQYSLSDNAQLLGRPTGFEVNIRQVK 513

HSP 2 Score: 28.8758 bits (63), Expect = 2.962e-19
Identity = 12/22 (54.55%), Postives = 15/22 (68.18%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGL 404
            + S GAGF+  L G + TMPGL
Sbjct:  513 KLSAGAGFVVALAGDIMTMPGL 534
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_SHEB5 (Formate--tetrahydrofolate ligase OS=Shewanella baltica (strain OS155 / ATCC BAA-1091) GN=fhs PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 3.848e-19
Identity = 44/102 (43.14%), Postives = 63/102 (61.76%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIAR-SYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDV 337
            G  GA+ L   V RA E  +Q  K LYP + S++ K+ T+A   YGA+GV  S EA++Q +  T  G+  LP+CMA T  S SHD + KG P  F++P+R++
Sbjct:  442 GEAGAIALAQTVVRAAETESQ-FKLLYPDEASLEAKLSTLAEVGYGATGVSLSIEAKQQAQQLTALGYGHLPLCMAKTPLSISHDPSLKGVPKDFVVPVREL 542

HSP 2 Score: 33.4982 bits (75), Expect = 3.848e-19
Identity = 15/26 (57.69%), Postives = 19/26 (73.08%), Query Frame = 3 
Query:  351 GAGFIYPLVGTMSTMPGLPIRPCLLR 428
            GAGFI  LVG + TMPGL ++P  L+
Sbjct:  547 GAGFITALVGNVMTMPGLGLKPGYLK 572
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_SYMTH (Formate--tetrahydrofolate ligase OS=Symbiobacterium thermophilum GN=fhs PE=3 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 3.852e-19
Identity = 45/105 (42.86%), Postives = 66/105 (62.86%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQP--LKFLYPLDVSIKEKIDTIA-RSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG G ++L    QR  E V QP   +F Y  + S+K KI+ +A R YGA GV+++ EA + ++    +GF   P+C+A TQYSFS D  + GAPTG+ L +R+V+
Sbjct:  413 GGDGGIELA---QRVVELVEQPNRFRFAYDDEDSLKTKIEKVATRIYGADGVDFTREASRMLKKLESEGFGRAPVCIAKTQYSFSDDPKKLGAPTGWRLTVREVR 514

HSP 2 Score: 33.113 bits (74), Expect = 3.852e-19
Identity = 15/26 (57.69%), Postives = 17/26 (65.38%), Query Frame = 3 
Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGF+  L G + TMPGLP  P
Sbjct:  514 RPSAGAGFVVALTGEIMTMPGLPPVP 539
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_STAAW (Formate--tetrahydrofolate ligase OS=Staphylococcus aureus (strain MW2) GN=fhs PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 5.009e-19
Identity = 45/106 (42.45%), Postives = 66/106 (62.26%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKF--LYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            GGKG VDL   V    E + QP  F  LY L++ +++KI+ I    YG S V +S +A+KQ++ +   G+   P+CMA TQYSFS D    GAP+GF + IR++++
Sbjct:  412 GGKGGVDLANEV---LEVIDQPNSFKPLYELELPLEQKIEKIVTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEITIRELEA 514

HSP 2 Score: 35.4242 bits (80), Expect = 5.009e-19
Identity = 16/28 (57.14%), Postives = 18/28 (64.29%), Query Frame = 3 
Query:  342 ASIGAGFIYPLVGTMSTMPGLPIRPCLL 425
            A  GAGFI  L G + TMPGLP +P  L
Sbjct:  514 AKTGAGFIVALTGAIMTMPGLPKKPAAL 541
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_STAAT (Formate--tetrahydrofolate ligase OS=Staphylococcus aureus (strain USA300 / TCH1516) GN=fhs PE=3 SV=2)

HSP 1 Score: 80.4925 bits (197), Expect = 5.009e-19
Identity = 45/106 (42.45%), Postives = 66/106 (62.26%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKF--LYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            GGKG VDL   V    E + QP  F  LY L++ +++KI+ I    YG S V +S +A+KQ++ +   G+   P+CMA TQYSFS D    GAP+GF + IR++++
Sbjct:  412 GGKGGVDLANEV---LEVIDQPNSFKPLYELELPLEQKIEKIVTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEITIRELEA 514

HSP 2 Score: 35.4242 bits (80), Expect = 5.009e-19
Identity = 16/28 (57.14%), Postives = 18/28 (64.29%), Query Frame = 3 
Query:  342 ASIGAGFIYPLVGTMSTMPGLPIRPCLL 425
            A  GAGFI  L G + TMPGLP +P  L
Sbjct:  514 AKTGAGFIVALTGAIMTMPGLPKKPAAL 541
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_STAAS (Formate--tetrahydrofolate ligase OS=Staphylococcus aureus (strain MSSA476) GN=fhs PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 5.009e-19
Identity = 45/106 (42.45%), Postives = 66/106 (62.26%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKF--LYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            GGKG VDL   V    E + QP  F  LY L++ +++KI+ I    YG S V +S +A+KQ++ +   G+   P+CMA TQYSFS D    GAP+GF + IR++++
Sbjct:  412 GGKGGVDLANEV---LEVIDQPNSFKPLYELELPLEQKIEKIVTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEITIRELEA 514

HSP 2 Score: 35.4242 bits (80), Expect = 5.009e-19
Identity = 16/28 (57.14%), Postives = 18/28 (64.29%), Query Frame = 3 
Query:  342 ASIGAGFIYPLVGTMSTMPGLPIRPCLL 425
            A  GAGFI  L G + TMPGLP +P  L
Sbjct:  514 AKTGAGFIVALTGAIMTMPGLPKKPAAL 541
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_STAAR (Formate--tetrahydrofolate ligase OS=Staphylococcus aureus (strain MRSA252) GN=fhs PE=3 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 5.009e-19
Identity = 45/106 (42.45%), Postives = 66/106 (62.26%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKF--LYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            GGKG VDL   V    E + QP  F  LY L++ +++KI+ I    YG S V +S +A+KQ++ +   G+   P+CMA TQYSFS D    GAP+GF + IR++++
Sbjct:  412 GGKGGVDLANEV---LEVIDQPNSFKPLYELELPLEQKIEKIVTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEITIRELEA 514

HSP 2 Score: 35.4242 bits (80), Expect = 5.009e-19
Identity = 16/28 (57.14%), Postives = 18/28 (64.29%), Query Frame = 3 
Query:  342 ASIGAGFIYPLVGTMSTMPGLPIRPCLL 425
            A  GAGFI  L G + TMPGLP +P  L
Sbjct:  514 AKTGAGFIVALTGAIMTMPGLPKKPAAL 541
[Close]
BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_STAAN (Formate--tetrahydrofolate ligase OS=Staphylococcus aureus (strain N315) GN=fhs PE=1 SV=1)

HSP 1 Score: 80.4925 bits (197), Expect = 5.009e-19
Identity = 45/106 (42.45%), Postives = 66/106 (62.26%), Query Frame = 2 
Query:   35 GGKGAVDLGIAVQRACENVTQPLKF--LYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVKS 343
            GGKG VDL   V    E + QP  F  LY L++ +++KI+ I    YG S V +S +A+KQ++ +   G+   P+CMA TQYSFS D    GAP+GF + IR++++
Sbjct:  412 GGKGGVDLANEV---LEVIDQPNSFKPLYELELPLEQKIEKIVTEIYGGSKVTFSSKAQKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEITIRELEA 514

HSP 2 Score: 35.4242 bits (80), Expect = 5.009e-19
Identity = 16/28 (57.14%), Postives = 18/28 (64.29%), Query Frame = 3 
Query:  342 ASIGAGFIYPLVGTMSTMPGLPIRPCLL 425
            A  GAGFI  L G + TMPGLP +P  L
Sbjct:  514 AKTGAGFIVALTGAIMTMPGLPKKPAAL 541
The following BLAST results are available for this feature:
BLAST of EY679003 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 293
Match NameE-valueIdentityDescription
FTHS_ARTAT2.961e-1944.12Formate--tetrahydrofolate ligase OS=Arthrobacter a... [more]
FTHS_BACLD2.962e-1941.75Formate--tetrahydrofolate ligase OS=Bacillus liche... [more]
FTHS_SYNWW2.962e-1946.60Formate--tetrahydrofolate ligase OS=Syntrophomonas... [more]
FTHS_SHEB53.848e-1943.14Formate--tetrahydrofolate ligase OS=Shewanella bal... [more]
FTHS_SYMTH3.852e-1942.86Formate--tetrahydrofolate ligase OS=Symbiobacteriu... [more]
FTHS_STAAW5.009e-1942.45Formate--tetrahydrofolate ligase OS=Staphylococcus... [more]
FTHS_STAAT5.009e-1942.45Formate--tetrahydrofolate ligase OS=Staphylococcus... [more]
FTHS_STAAS5.009e-1942.45Formate--tetrahydrofolate ligase OS=Staphylococcus... [more]
FTHS_STAAR5.009e-1942.45Formate--tetrahydrofolate ligase OS=Staphylococcus... [more]
FTHS_STAAN5.009e-1942.45Formate--tetrahydrofolate ligase OS=Staphylococcus... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-053-H07-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY679003 ID=EY679003; Name=EY679003; organism=Citrus sinensis; type=EST; length=876bp
GCCAGTTTAGTTTTCGTTCTCACCATCGCCCATGGGTGGAAAAGGAGCAG
TGGACCTTGGAATTGCAGTTCAAAGAGCCTGTGAGAATGTTACACAGCCA
TTGAAGTTCTTATATCCTTTAGATGTCAGTATTAAAGAGAAAATAGATAC
AATAGCAAGGTCATATGGTGCTAGCGGCGTTGAGTACTCAGAGGAGGCTG
AGAAACAGATCGAAATGTACACCGGGCAAGGGTTTTCTGGCTTACCAATT
TGCATGGCATAAACCCAGTATTCATTTTCACACGATGCCGCCGAGAAGGG
AGCCCCAACTGGGTTTATCTTACCGATAAGGGATGTAAAGAGCTAGCATT
GGGGCTGGATTCATTTATCCTTTGGTTGGAACAATGAGTACAATGCCAGG
TCTCCCAATAAGGCCATGCCTTTTAAGAGAATGGAGTCAACACTGCAACT
GGAAAGGTTGTTGGGGCTGTCTTAAGATCCCACAAAGCACTCCCGTGTGG
GTCCTCAAGAAGCTCTCCTTTAGATGTTCTTAAATGCTCCTGAGCAAACA
ATGAGCTTGAAGGGCTCCCCCCCCCCCCCACTGGCTGATATTCTAACCCC
GATCTGTAACACTTATGGGTACCTCGGGCTATTTGCTGTGGTAAACAAAA
CCCGACACCAAATAGAACCTGGAAAAAAAAAATCTTGGTACTCACCTTGC
GGACTTTAATTTTCCCTATTAATGTGTCCTTACTCCCATTTGATCTTGAA
TCCCGTTACGAAAATTTACCCTATGGGTGGGGGTGTGATATTTGTAAAAT
AAACAAAAAAAAAAAAAAAATAAAATAAAAGCAAAAGGGCGCGGGCGCGG
GTTAATAAATTATGAAAGGTATCAGA
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