EY679003 | Citrus Genome Database

Overview


Name	EY679003
Unique Name	EY679003
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	876

Libraries


Library Name	Type
Sweet orange leaf, infected with CiLV	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_CLOBM (Formate--tetrahydrofolate ligase OS=Clostridium botulinum (strain Loch Maree / Type A3) GN=fhs PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.862e-18
Identity = 44/103 (42.72%), Postives = 60/103 (58.25%), Query Frame = 2

Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG G ++L   V    EN       +Y   ++IK+KI+TIA+  YGA GV+YS+EAEKQI           P+CMA TQYS S DA   G P GF + +++V+
Sbjct:  413 GGNGGLELADKVLDTIENKESKFNPIYEETLNIKQKIETIAQEIYGAEGVDYSKEAEKQISEIEKLDLDKKPVCMAKTQYSLSDDAKLLGRPCGFRINVKEVR 515

HSP 2 Score: 34.6538 bits (78), Expect = 1.862e-18
Identity = 16/26 (61.54%), Postives = 18/26 (69.23%), Query Frame = 3

Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGFI  L G + TMPGLP +P
Sbjct:  515 RISNGAGFIVVLTGNVMTMPGLPKKP 540

[Close]

BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_CLOBL (Formate--tetrahydrofolate ligase OS=Clostridium botulinum (strain Langeland / NCTC 10281 / Type F) GN=fhs PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.862e-18
Identity = 44/103 (42.72%), Postives = 60/103 (58.25%), Query Frame = 2

Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG G ++L   V    EN       +Y   ++IK+KI+TIA+  YGA GV+YS+EAEKQI           P+CMA TQYS S DA   G P GF + +++V+
Sbjct:  413 GGNGGLELADKVLDTIENKESKFNPIYEETLNIKQKIETIAQEIYGAEGVDYSKEAEKQISEIEKLDLDKKPVCMAKTQYSLSDDAKLLGRPCGFRINVKEVR 515

HSP 2 Score: 34.6538 bits (78), Expect = 1.862e-18
Identity = 16/26 (61.54%), Postives = 18/26 (69.23%), Query Frame = 3

Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGFI  L G + TMPGLP +P
Sbjct:  515 RISNGAGFIVVLTGNVMTMPGLPKKP 540

[Close]

BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_CLOBK (Formate--tetrahydrofolate ligase OS=Clostridium botulinum (strain Okra / Type B1) GN=fhs PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.862e-18
Identity = 44/103 (42.72%), Postives = 60/103 (58.25%), Query Frame = 2

Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG G ++L   V    EN       +Y   ++IK+KI+TIA+  YGA GV+YS+EAEKQI           P+CMA TQYS S DA   G P GF + +++V+
Sbjct:  413 GGNGGLELADKVLDTIENKESKFNPIYEETLNIKQKIETIAQEIYGAEGVDYSKEAEKQISEIEKLDLDKKPVCMAKTQYSLSDDAKLLGRPCGFRINVKEVR 515

HSP 2 Score: 34.6538 bits (78), Expect = 1.862e-18
Identity = 16/26 (61.54%), Postives = 18/26 (69.23%), Query Frame = 3

Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGFI  L G + TMPGLP +P
Sbjct:  515 RISNGAGFIVVLTGNVMTMPGLPKKP 540

[Close]

BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_CLOBJ (Formate--tetrahydrofolate ligase OS=Clostridium botulinum (strain Kyoto / Type A2) GN=fhs PE=3 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.862e-18
Identity = 44/103 (42.72%), Postives = 60/103 (58.25%), Query Frame = 2

Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG G ++L   V    EN       +Y   ++IK+KI+TIA+  YGA GV+YS+EAEKQI           P+CMA TQYS S DA   G P GF + +++V+
Sbjct:  413 GGNGGLELADKVLDTIENKESKFNPIYEETLNIKQKIETIAQEIYGAEGVDYSKEAEKQISEIEKLDLDKKPVCMAKTQYSLSDDARLLGRPCGFRINVKEVR 515

HSP 2 Score: 34.6538 bits (78), Expect = 1.862e-18
Identity = 16/26 (61.54%), Postives = 18/26 (69.23%), Query Frame = 3

Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGFI  L G + TMPGLP +P
Sbjct:  515 RISNGAGFIVVLTGNVMTMPGLPKKP 540

[Close]

BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_NOCSJ (Formate--tetrahydrofolate ligase OS=Nocardioides sp. (strain BAA-499 / JS614) GN=fhs PE=3 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 2.421e-18
Identity = 43/104 (41.35%), Postives = 61/104 (58.65%), Query Frame = 2

Query:   35 GGKGAVDLGIAVQRACENVTQP-LKFLYPLDVSIKEKIDTIA-RSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG GA DL   V +A E   +    F YP ++S+ EK++ +A R YGA  V +  +A K++      G+  LP+C+A TQYSFS D    GAPTG  L +R+V+
Sbjct:  414 GGIGAQDLAKGVLQALEEPARDEFSFTYPDELSLTEKVEAVATRVYGAGQVTWDGKARKRLARIERDGYGTLPVCVAKTQYSFSTDPGLLGAPTGHELRVREVR 517

HSP 2 Score: 38.1206 bits (87), Expect = 2.421e-18
Identity = 17/30 (56.67%), Postives = 19/30 (63.33%), Query Frame = 3

Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPCLLR 428
            R S GAGF+  + G M TMPGLP RP   R
Sbjct:  517 RLSAGAGFVVVICGDMMTMPGLPTRPAATR 546

[Close]

BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: C1TC_DROME (C-1-tetrahydrofolate synthase, cytoplasmic OS=Drosophila melanogaster GN=pug PE=1 SV=4)

HSP 1 Score: 79.7221 bits (195), Expect = 3.113e-18
Identity = 46/102 (45.10%), Postives = 62/102 (60.78%), Query Frame = 2

Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDV 337
            GG GAV L  AV +ACE   Q  + LY L++ + +K++ IA + YGA  V  S  AE++++  T  GF  LPICM+    SF+ DA  KGAP GF L + DV
Sbjct:  824 GGAGAVQLADAVIKACEQGNQ-FRLLYDLELPLVDKMNKIATTMYGAGKVVLSPAAEEKVKRLTDAGFGNLPICMSKVSGSFTGDAKIKGAPKGFTLDVEDV 924

HSP 2 Score: 33.4982 bits (75), Expect = 3.113e-18
Identity = 13/26 (50.00%), Postives = 18/26 (69.23%), Query Frame = 3

Query:  345 SIGAGFIYPLVGTMSTMPGLPIRPCL 422
            S GAGF+  + G ++ MPGLP RP +
Sbjct:  927 SAGAGFVVAMCGEVTKMPGLPTRPAI 952

[Close]

BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_SHEON (Formate--tetrahydrofolate ligase OS=Shewanella oneidensis GN=fhs PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 3.146e-18
Identity = 41/102 (40.20%), Postives = 65/102 (63.73%), Query Frame = 2

Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIAR-SYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDV 337
            G  GA+ L   V RACE  ++  + LYP ++ ++ K+ T+A   YGA+G+  S  A++Q++  +  G++ LP+CMA T  S SHD   KG P GFI+P+R++
Sbjct:  427 GEDGAMALAQTVIRACEQPSE-FRLLYPDNMELEAKLSTLAELGYGAAGINLSAVAKQQLQELSALGYAHLPVCMAKTPLSISHDPQLKGVPQGFIVPVREL 527

HSP 2 Score: 32.3426 bits (72), Expect = 3.146e-18
Identity = 15/26 (57.69%), Postives = 18/26 (69.23%), Query Frame = 3

Query:  351 GAGFIYPLVGTMSTMPGLPIRPCLLR 428
            GAGFI  LVG + TMPGL + P  L+
Sbjct:  532 GAGFITALVGNVMTMPGLGLTPGYLK 557

[Close]

BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_METEA (Formate--tetrahydrofolate ligase OS=Methylobacterium extorquens (strain ATCC 14718 / DSM 1338 / AM1) GN=fhs PE=1 SV=3)

HSP 1 Score: 81.6481 bits (200), Expect = 3.148e-18
Identity = 42/103 (40.78%), Postives = 61/103 (59.22%), Query Frame = 2

Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIA-RSYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG GA  L  AV +  E   +PL F Y  +  I +KI  IA + YGA+ ++   +A  ++  +   G+ GLP+CMA TQYSFS D    GAP+G ++ +RDV+
Sbjct:  413 GGAGAEALAQAVVKLAEGEQKPLTFAYETETKITDKIKAIATKLYGAADIQIESKAATKLAGFEKDGYGGLPVCMAKTQYSFSTDPTLMGAPSGHLVSVRDVR 515

HSP 2 Score: 31.5722 bits (70), Expect = 3.148e-18
Identity = 14/26 (53.85%), Postives = 17/26 (65.38%), Query Frame = 3

Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGF+  + G + TMPGLP  P
Sbjct:  515 RLSAGAGFVVVICGEIMTMPGLPKVP 540

[Close]

BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_CLOAB (Formate--tetrahydrofolate ligase OS=Clostridium acetobutylicum GN=fhs PE=3 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 3.148e-18
Identity = 45/103 (43.69%), Postives = 61/103 (59.22%), Query Frame = 2

Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIARS-YGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG+G  DL   V    EN     K+LY   +SIKEK+D IA+  YGA GV+Y+ +A KQI+         LPIC+A TQYS S + A  G PT F + +++V+
Sbjct:  412 GGEGGTDLANKVLDTLENEKSNFKYLYDEKLSIKEKMDIIAKEIYGADGVQYTPQANKQIKEIEKFNLDKLPICVAKTQYSLSDNPALLGRPTNFTINVKEVR 514

HSP 2 Score: 31.187 bits (69), Expect = 3.148e-18
Identity = 14/26 (53.85%), Postives = 16/26 (61.54%), Query Frame = 3

Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRP 416
            R S GAGF+    G + TMPGLP  P
Sbjct:  514 RVSNGAGFVVVQTGNIMTMPGLPKTP 539

[Close]

BLAST of EY679003 vs. ExPASy Swiss-Prot
Match: FTHS_VIBHB (Formate--tetrahydrofolate ligase OS=Vibrio harveyi (strain ATCC BAA-1116 / BB120) GN=fhs PE=3 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 5.317e-18
Identity = 42/103 (40.78%), Postives = 63/103 (61.17%), Query Frame = 2

Query:   35 GGKGAVDLGIAVQRACENVTQPLKFLYPLDVSIKEKIDTIAR-SYGASGVEYSEEAEKQIEMYTGQGFSGLPICMA*TQYSFSHDAAEKGAPTGFILPIRDVK 340
            GG+GA++L   V   CE   Q  + LY    S+KEK+  +    YGAS +E SE A++Q+  +   GF  L +C+A T  S + D+A KGAP+GF +PIR+++
Sbjct:  439 GGEGAIELAQHVVETCEQGAQ-FRPLYTKKQSLKEKLMAVCEVGYGASNIEMSELAKQQLAHFEKLGFDDLAVCIAKTPLSITTDSAIKGAPSGFAVPIRELR 540

HSP 2 Score: 36.1946 bits (82), Expect = 5.317e-18
Identity = 15/29 (51.72%), Postives = 19/29 (65.52%), Query Frame = 3

Query:  339 RASIGAGFIYPLVGTMSTMPGLPIRPCLL 425
            R   GAGF+Y L G + TMPGLP +P  +
Sbjct:  540 RLCAGAGFVYALSGNVMTMPGLPDKPTFM 568

The following BLAST results are available for this feature:

BLAST of EY679003 vs. ExPASy Swiss-Prot

BLAST of EY679003 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 293


Match Name	E-value	Identity	Description
FTHS_CLOBM	1.862e-18	42.72	Formate--tetrahydrofolate ligase OS=Clostridium bo...	[more]
FTHS_CLOBL	1.862e-18	42.72	Formate--tetrahydrofolate ligase OS=Clostridium bo...	[more]
FTHS_CLOBK	1.862e-18	42.72	Formate--tetrahydrofolate ligase OS=Clostridium bo...	[more]
FTHS_CLOBJ	1.862e-18	42.72	Formate--tetrahydrofolate ligase OS=Clostridium bo...	[more]
FTHS_NOCSJ	2.421e-18	41.35	Formate--tetrahydrofolate ligase OS=Nocardioides s...	[more]
C1TC_DROME	3.113e-18	45.10	C-1-tetrahydrofolate synthase, cytoplasmic OS=Dros...	[more]
FTHS_SHEON	3.146e-18	40.20	Formate--tetrahydrofolate ligase OS=Shewanella one...	[more]
FTHS_METEA	3.148e-18	40.78	Formate--tetrahydrofolate ligase OS=Methylobacteri...	[more]
FTHS_CLOAB	3.148e-18	43.69	Formate--tetrahydrofolate ligase OS=Clostridium ac...	[more]
FTHS_VIBHB	5.317e-18	40.78	Formate--tetrahydrofolate ligase OS=Vibrio harveyi...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C1-401-053-H07-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY679003 ID=EY679003; Name=EY679003; organism=Citrus sinensis; type=EST; length=876bp
GCCAGTTTAGTTTTCGTTCTCACCATCGCCCATGGGTGGAAAAGGAGCAG
TGGACCTTGGAATTGCAGTTCAAAGAGCCTGTGAGAATGTTACACAGCCA
TTGAAGTTCTTATATCCTTTAGATGTCAGTATTAAAGAGAAAATAGATAC
AATAGCAAGGTCATATGGTGCTAGCGGCGTTGAGTACTCAGAGGAGGCTG
AGAAACAGATCGAAATGTACACCGGGCAAGGGTTTTCTGGCTTACCAATT
TGCATGGCATAAACCCAGTATTCATTTTCACACGATGCCGCCGAGAAGGG
AGCCCCAACTGGGTTTATCTTACCGATAAGGGATGTAAAGAGCTAGCATT
GGGGCTGGATTCATTTATCCTTTGGTTGGAACAATGAGTACAATGCCAGG
TCTCCCAATAAGGCCATGCCTTTTAAGAGAATGGAGTCAACACTGCAACT
GGAAAGGTTGTTGGGGCTGTCTTAAGATCCCACAAAGCACTCCCGTGTGG
GTCCTCAAGAAGCTCTCCTTTAGATGTTCTTAAATGCTCCTGAGCAAACA
ATGAGCTTGAAGGGCTCCCCCCCCCCCCCACTGGCTGATATTCTAACCCC
GATCTGTAACACTTATGGGTACCTCGGGCTATTTGCTGTGGTAAACAAAA
CCCGACACCAAATAGAACCTGGAAAAAAAAAATCTTGGTACTCACCTTGC
GGACTTTAATTTTCCCTATTAATGTGTCCTTACTCCCATTTGATCTTGAA
TCCCGTTACGAAAATTTACCCTATGGGTGGGGGTGTGATATTTGTAAAAT
AAACAAAAAAAAAAAAAAAATAAAATAAAAGCAAAAGGGCGCGGGCGCGG
GTTAATAAATTATGAAAGGTATCAGA