EY665235

Overview
NameEY665235
Unique NameEY665235
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1063
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY665235 vs. ExPASy Swiss-Prot
Match: UB2G1_RAT (Ubiquitin-conjugating enzyme E2 G1 OS=Rattus norvegicus GN=Ube2g1 PE=2 SV=3)

HSP 1 Score: 70.4774 bits (171), Expect = 3.172e-12
Identity = 30/77 (38.96%), Postives = 51/77 (66.23%), Query Frame = 1 
Query:  148 ETMASKRILKELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFMTKVF 375
            E  ++  + ++L +L K+P    SAG + + D++ W+  I+GPPD+ Y GGVF   + FP DYP +PPK+ F+T+++
Sbjct:    3 ELQSALLLRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLYEGGVFKAHLTFPKDYPLRPPKMKFITEIW 79

HSP 2 Score: 22.7126 bits (47), Expect = 3.172e-12
Identity = 7/15 (46.67%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  387 LNSNGSICLDILKEP 431
            ++ NG +C+ IL EP
Sbjct:   83 VDKNGDVCISILHEP 97
[Close]
BLAST of EY665235 vs. ExPASy Swiss-Prot
Match: UB2G1_MOUSE (Ubiquitin-conjugating enzyme E2 G1 OS=Mus musculus GN=Ube2g1 PE=2 SV=3)

HSP 1 Score: 70.4774 bits (171), Expect = 3.172e-12
Identity = 30/77 (38.96%), Postives = 51/77 (66.23%), Query Frame = 1 
Query:  148 ETMASKRILKELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFMTKVF 375
            E  ++  + ++L +L K+P    SAG + + D++ W+  I+GPPD+ Y GGVF   + FP DYP +PPK+ F+T+++
Sbjct:    3 ELQSALLLRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLYEGGVFKAHLTFPKDYPLRPPKMKFITEIW 79

HSP 2 Score: 22.7126 bits (47), Expect = 3.172e-12
Identity = 7/15 (46.67%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  387 LNSNGSICLDILKEP 431
            ++ NG +C+ IL EP
Sbjct:   83 VDKNGDVCISILHEP 97
[Close]
BLAST of EY665235 vs. ExPASy Swiss-Prot
Match: UB2G1_MACFA (Ubiquitin-conjugating enzyme E2 G1 OS=Macaca fascicularis GN=UBE2G1 PE=2 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 3.172e-12
Identity = 30/77 (38.96%), Postives = 51/77 (66.23%), Query Frame = 1 
Query:  148 ETMASKRILKELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFMTKVF 375
            E  ++  + ++L +L K+P    SAG + + D++ W+  I+GPPD+ Y GGVF   + FP DYP +PPK+ F+T+++
Sbjct:    3 ELQSALLLRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLYEGGVFKAHLTFPKDYPLRPPKMKFITEIW 79

HSP 2 Score: 22.7126 bits (47), Expect = 3.172e-12
Identity = 7/15 (46.67%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  387 LNSNGSICLDILKEP 431
            ++ NG +C+ IL EP
Sbjct:   83 VDKNGDVCISILHEP 97
[Close]
BLAST of EY665235 vs. ExPASy Swiss-Prot
Match: UB2G1_HUMAN (Ubiquitin-conjugating enzyme E2 G1 OS=Homo sapiens GN=UBE2G1 PE=1 SV=3)

HSP 1 Score: 70.4774 bits (171), Expect = 3.172e-12
Identity = 30/77 (38.96%), Postives = 51/77 (66.23%), Query Frame = 1 
Query:  148 ETMASKRILKELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFMTKVF 375
            E  ++  + ++L +L K+P    SAG + + D++ W+  I+GPPD+ Y GGVF   + FP DYP +PPK+ F+T+++
Sbjct:    3 ELQSALLLRRQLAELNKNPVEGFSAGLIDDNDLYRWEVLIIGPPDTLYEGGVFKAHLTFPKDYPLRPPKMKFITEIW 79

HSP 2 Score: 22.7126 bits (47), Expect = 3.172e-12
Identity = 7/15 (46.67%), Postives = 11/15 (73.33%), Query Frame = 3 
Query:  387 LNSNGSICLDILKEP 431
            ++ NG +C+ IL EP
Sbjct:   83 VDKNGDVCISILHEP 97
[Close]
BLAST of EY665235 vs. ExPASy Swiss-Prot
Match: UBC15_SCHPO (Ubiquitin-conjugating enzyme E2 15 OS=Schizosaccharomyces pombe GN=ubc15 PE=2 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 5.056e-12
Identity = 34/75 (45.33%), Postives = 51/75 (68.00%), Query Frame = 1 
Query:  157 ASKRIL-KELKDLQKDPPTSCSAGPVAE-DMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFMTKVF 375
            AS+++L K+LK++QK+PP   S G V +  +F W+  I+GP D+ Y GG F  T+ FP DYP  PPK+ F T+++
Sbjct:    5 ASEQLLRKQLKEIQKNPPQGFSVGLVDDKSIFEWEVMIIGPEDTLYEGGFFHATLSFPQDYPLMPPKMKFTTEIW 79
[Close]
BLAST of EY665235 vs. ExPASy Swiss-Prot
Match: UBC6_CANGA (Ubiquitin-conjugating enzyme E2 6 OS=Candida glabrata GN=UBC6 PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.471e-11
Identity = 31/70 (44.29%), Postives = 43/70 (61.43%), Query Frame = 1 
Query:  157 ASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFMT 366
            A KR+ KE K + ++PP    A P  E++  W   I GPPD+PY GG +  T+ FP DYP+KPP +  +T
Sbjct:    6 AQKRLTKEYKMMVENPPPFIIARPNEENILEWHYVISGPPDTPYDGGQYHGTLTFPSDYPYKPPAIRMIT 75
[Close]
BLAST of EY665235 vs. ExPASy Swiss-Prot
Match: UBC2_CRYNE (Ubiquitin-conjugating enzyme E2 2 OS=Cryptococcus neoformans GN=UBC2 PE=3 SV=1)

HSP 1 Score: 70.4774 bits (171), Expect = 1.921e-11
Identity = 34/97 (35.05%), Postives = 54/97 (55.67%), Query Frame = 1 
Query:  145 VETMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFMTKVFSS*H*IAMGAFALTS*KNQW 435
            + T A +R++++ K L  D P   S  P  +++  W A I GPP++P+  G F +T+ F   YP KPP V F++K+F   +  A G   L   +N+W
Sbjct:    1 MSTAAKRRLIRDFKRLTSDAPIGISGSPNPDNIMVWNAVIFGPPETPFEDGSFRLTLTFTDAYPNKPPTVRFISKMFHP-NIYANGELCLDILQNRW 96
[Close]
BLAST of EY665235 vs. ExPASy Swiss-Prot
Match: UBC13_SCHPO (Ubiquitin-conjugating enzyme E2 13 OS=Schizosaccharomyces pombe GN=ubc13 PE=1 SV=1)

HSP 1 Score: 69.707 bits (169), Expect = 3.277e-11
Identity = 32/91 (35.16%), Postives = 53/91 (58.24%), Query Frame = 1 
Query:  163 KRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFMTKVFSS*H*IAMGAFALTS*KNQW 435
            KRI+KE++ L +DPP    A P  +++ +++ T+ GP  S Y GG F + +  P +YP  PP V F+TK++   +   +G   L++ K  W
Sbjct:    5 KRIIKEIETLTRDPPPGIVAAPTEDNLRYFKITMEGPQQSAYEGGKFHLELFLPDEYPMMPPNVRFLTKIYHP-NVDKLGRICLSTLKKDW 94
[Close]
BLAST of EY665235 vs. ExPASy Swiss-Prot
Match: UB2J1_MOUSE (Ubiquitin-conjugating enzyme E2 J1 OS=Mus musculus GN=Ube2j1 PE=1 SV=2)

HSP 1 Score: 69.3218 bits (168), Expect = 4.280e-11
Identity = 31/74 (41.89%), Postives = 47/74 (63.51%), Query Frame = 1 
Query:  145 VETMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFMT 366
            +++ A KR++KE  +L KDP     A P+ +++F W  T+ GPPDS + GGV+   I  PP+YP KPP +  +T
Sbjct:    7 LKSPAVKRLMKEAAEL-KDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLT 79
[Close]
BLAST of EY665235 vs. ExPASy Swiss-Prot
Match: UB2J1_HUMAN (Ubiquitin-conjugating enzyme E2 J1 OS=Homo sapiens GN=UBE2J1 PE=1 SV=2)

HSP 1 Score: 69.3218 bits (168), Expect = 4.280e-11
Identity = 31/74 (41.89%), Postives = 47/74 (63.51%), Query Frame = 1 
Query:  145 VETMASKRILKELKDLQKDPPTSCSAGPVAEDMFHWQATIMGPPDSPYAGGVFLVTIHFPPDYPFKPPKVAFMT 366
            +++ A KR++KE  +L KDP     A P+ +++F W  T+ GPPDS + GGV+   I  PP+YP KPP +  +T
Sbjct:    7 LKSPAVKRLMKEAAEL-KDPTDHYHAQPLEDNLFEWHFTVRGPPDSDFDGGVYHGRIVLPPEYPMKPPSIILLT 79
The following BLAST results are available for this feature:
BLAST of EY665235 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 102
Match NameE-valueIdentityDescription
UB2G1_RAT3.172e-1238.96Ubiquitin-conjugating enzyme E2 G1 OS=Rattus norve... [more]
UB2G1_MOUSE3.172e-1238.96Ubiquitin-conjugating enzyme E2 G1 OS=Mus musculus... [more]
UB2G1_MACFA3.172e-1238.96Ubiquitin-conjugating enzyme E2 G1 OS=Macaca fasci... [more]
UB2G1_HUMAN3.172e-1238.96Ubiquitin-conjugating enzyme E2 G1 OS=Homo sapiens... [more]
UBC15_SCHPO5.056e-1245.33Ubiquitin-conjugating enzyme E2 15 OS=Schizosaccha... [more]
UBC6_CANGA1.471e-1144.29Ubiquitin-conjugating enzyme E2 6 OS=Candida glabr... [more]
UBC2_CRYNE1.921e-1135.05Ubiquitin-conjugating enzyme E2 2 OS=Cryptococcus ... [more]
UBC13_SCHPO3.277e-1135.16Ubiquitin-conjugating enzyme E2 13 OS=Schizosaccha... [more]
UB2J1_MOUSE4.280e-1141.89Ubiquitin-conjugating enzyme E2 J1 OS=Mus musculus... [more]
UB2J1_HUMAN4.280e-1141.89Ubiquitin-conjugating enzyme E2 J1 OS=Homo sapiens... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-101-083-E10-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY665235 ID=EY665235; Name=EY665235; organism=Citrus sinensis; type=EST; length=1063bp
AATTTTTCTCCTGCTTCCATTTTTCCTTCGCCGTTCAATTTTTTTAAGTA
TCTCAGTTTTTGTTTCTAATTTTCCCGATCTCCCTGTCTCCCTCACGAAT
CCACATATTGCAAGGCCAAATCAGGAGGATCTGCTGCTTCTCAGGTTGAA
ACTATGGCGTCGAAACGGATCTTGAAGGAGCTCAAAGATTTGCAGAAGGA
TCCTCCTACATCATGCAGTGCCGGTCCTGTTGCTGAAGACATGTTTCATT
GGCAGGCGACAATCATGGGTCCTCCAGATAGTCCGTATGCTGGGGGTGTT
TTTCTAGTTACCATCCATTTTCCCCCAGACTATCCATTCAAGCCTCCCAA
GGTAGCATTCATGACAAAGGTATTTTCATCCTAACATTAAATAGCAATGG
GAGCATTTGCCTTGACATCTTGAAAGAACCAATGGGAGTCCTGCTCTTAC
CCATTTCCAAAGGGGTTGCTAACCAATCCTGGTTCATTATTTGACGGAAC
CAAATCCCGAATGGATCCGTTTGGGTAACCCAAAATTTGCTCACATGTTA
CAAAACCGACAGGGAACAAGTACAAAACCAACTGGAAGGGAACTGGAACC
CAAAAGTATGGCAATGGGCTAAACCCTGGGGTTTATGGGGGGGGAGGGGG
GGTCCCTTTTTCTTTTTTAATTCTGGGTTTTCAAGGTAAAGGAAAAAAAA
ATTCTGGACCGTGTCATATCCATAAAAAAGGTGGAAAATTAAAAAAAAGG
GTCCACATAATATCCCCCCCAAAATTGGAAAAAGGAAAAAAAACCGGGTT
AGATTTCTTAAAACTGGTAAAAAAGAGAAAAATGGTCACACTTGAGGTAC
AAAACAAAGAACTGGACTTTCACTAAAAATAGAGGTGTCCATTATGGACT
CCATCACTAAAATTTGTGTAATCGAACTTACTTTTTAAACAAAAATAAAG
TCAAAAAACAATTTTTATTATTTATTATAATAAGATAAAAAAAAAACAAA
ATAAAAGAAGAGAGGGGGGACGTACTGCTCATCNTATTTATCATGAGAAT
GATATCAATTGTA
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