EY665137 | Citrus Genome Database

Overview


Name	EY665137
Unique Name	EY665137
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	862

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY665137 vs. ExPASy Swiss-Prot
Match: NEDD8_CAEEL (NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.401e-19
Identity = 46/76 (60.53%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  103 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 330
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

HSP 2 Score: 97.0561 bits (240), Expect = 1.401e-19
Identity = 46/76 (60.53%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  331 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 558
            M I VKTLTGK I L++E +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T ADY +   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAADYKVLGGSVLHLVLALRGG 76

HSP 3 Score: 72.4034 bits (176), Expect = 3.695e-12
Identity = 33/57 (57.89%), Postives = 44/57 (77.19%), Query Frame = 1

Query:  559 MQIFVKTLTGKTITLEVXESDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLA 729
            M I VKTLTGK I L++  +D ++ +K K+++KEGIPP QQRLIFAGKQ+ D +T A
Sbjct:    1 MLIKVKTLTGKEIELDIEPNDRVERIKEKVEEKEGIPPPQQRLIFAGKQMNDDKTAA 57

[Close]

BLAST of EY665137 vs. ExPASy Swiss-Prot
Match: RUB2_ARATH (NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN=RUB2 PE=2 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 1.829e-19
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = 1

Query:  109 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 333
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

HSP 2 Score: 96.6709 bits (239), Expect = 1.829e-19
Identity = 46/75 (61.33%), Postives = 59/75 (78.67%), Query Frame = 1

Query:  337 IFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 561
            I VKTLTGK I +++E +DTID +K ++++KEGIPP QQRLI+AGKQL D +T  DY I+  S LHLVL LRGG+
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKTAKDYAIEGGSVLHLVLALRGGL 77

HSP 3 Score: 70.8626 bits (172), Expect = 1.075e-11
Identity = 32/53 (60.38%), Postives = 43/53 (81.13%), Query Frame = 1

Query:  565 IFVKTLTGKTITLEVXESDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRT 723
            I VKTLTGK I +++  +DTID +K ++++KEGIPP QQRLI+AGKQL D +T
Sbjct:    3 IKVKTLTGKEIEIDIEPTDTIDRIKERVEEKEGIPPVQQRLIYAGKQLADDKT 55

[Close]

BLAST of EY665137 vs. ExPASy Swiss-Prot
Match: NEDD8_RAT (NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 2.023e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  103 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 330
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 93.2041 bits (230), Expect = 2.023e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  331 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 558
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 3 Score: 68.1662 bits (165), Expect = 6.968e-11
Identity = 29/57 (50.88%), Postives = 44/57 (77.19%), Query Frame = 1

Query:  559 MQIFVKTLTGKTITLEVXESDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLA 729
            M I VKTLTGK I +++  +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T A
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAA 57

[Close]

BLAST of EY665137 vs. ExPASy Swiss-Prot
Match: NEDD8_RABIT (NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 2.023e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  103 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 330
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 93.2041 bits (230), Expect = 2.023e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  331 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 558
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 3 Score: 68.1662 bits (165), Expect = 6.968e-11
Identity = 29/57 (50.88%), Postives = 44/57 (77.19%), Query Frame = 1

Query:  559 MQIFVKTLTGKTITLEVXESDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLA 729
            M I VKTLTGK I +++  +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T A
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAA 57

[Close]

BLAST of EY665137 vs. ExPASy Swiss-Prot
Match: NEDD8_MOUSE (NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 2.023e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  103 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 330
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 93.2041 bits (230), Expect = 2.023e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  331 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 558
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 3 Score: 68.1662 bits (165), Expect = 6.968e-11
Identity = 29/57 (50.88%), Postives = 44/57 (77.19%), Query Frame = 1

Query:  559 MQIFVKTLTGKTITLEVXESDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLA 729
            M I VKTLTGK I +++  +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T A
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAA 57

[Close]

BLAST of EY665137 vs. ExPASy Swiss-Prot
Match: NEDD8_HUMAN (NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 2.023e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  103 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 330
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 93.2041 bits (230), Expect = 2.023e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  331 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 558
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 3 Score: 68.1662 bits (165), Expect = 6.968e-11
Identity = 29/57 (50.88%), Postives = 44/57 (77.19%), Query Frame = 1

Query:  559 MQIFVKTLTGKTITLEVXESDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLA 729
            M I VKTLTGK I +++  +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T A
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAA 57

[Close]

BLAST of EY665137 vs. ExPASy Swiss-Prot
Match: NEDD8_BOVIN (NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 2.023e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  103 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 330
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 2 Score: 93.2041 bits (230), Expect = 2.023e-18
Identity = 43/76 (56.58%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  331 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 558
            M I VKTLTGK I +++E +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T ADY I   S LHLVL LRGG
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGG 76

HSP 3 Score: 68.1662 bits (165), Expect = 6.968e-11
Identity = 29/57 (50.88%), Postives = 44/57 (77.19%), Query Frame = 1

Query:  559 MQIFVKTLTGKTITLEVXESDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLA 729
            M I VKTLTGK I +++  +D ++ +K ++++KEGIPP QQRLI++GKQ+ D +T A
Sbjct:    1 MLIKVKTLTGKEIEIDIEPTDKVERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAA 57

[Close]

BLAST of EY665137 vs. ExPASy Swiss-Prot
Match: RUB3_ORYSJ (NEDD8-like protein RUB3 OS=Oryza sativa subsp. japonica GN=RUB3 PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.642e-18
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  103 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 330
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

HSP 2 Score: 92.8189 bits (229), Expect = 2.642e-18
Identity = 41/76 (53.95%), Postives = 58/76 (76.32%), Query Frame = 1

Query:  331 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 558
            + + V+TLTGK I +++E +DT+D +K +++++EGIPP QQRLI+ GKQL D +T  DY I+  S LHLVL LRGG
Sbjct:    1 LNVKVRTLTGKEIDIDIEMTDTVDRIKERVEEREGIPPVQQRLIYGGKQLADDKTAHDYKIEAGSVLHLVLALRGG 76

[Close]

BLAST of EY665137 vs. ExPASy Swiss-Prot
Match: RUB3_ARATH (NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN=RUB3 PE=2 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 1.712e-17
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 1

Query:  103 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 330
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

HSP 2 Score: 90.1225 bits (222), Expect = 1.712e-17
Identity = 42/76 (55.26%), Postives = 57/76 (75.00%), Query Frame = 1

Query:  331 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGG 558
            M+I VKTLT K I +E+E +DTI+ +K +I++KEGIPP  QR+++ GKQL D  T   YN+++ S LHLVL LRGG
Sbjct:    1 MEIKVKTLTEKQIDIEIELTDTIERIKERIEEKEGIPPVHQRIVYTGKQLADDLTAKHYNLERGSVLHLVLALRGG 76

[Close]

BLAST of EY665137 vs. ExPASy Swiss-Prot
Match: NEDD8_DICDI (NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV=2)

HSP 1 Score: 89.7373 bits (221), Expect = 2.236e-17
Identity = 41/77 (53.25%), Postives = 58/77 (75.32%), Query Frame = 1

Query:  103 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 333
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77

HSP 2 Score: 89.7373 bits (221), Expect = 2.236e-17
Identity = 41/77 (53.25%), Postives = 58/77 (75.32%), Query Frame = 1

Query:  331 MQIFVKTLTGKTITLEVESSDTIDNVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLADYNIQKESTLHLVLRLRGGM 561
            M I VKTLTGK I ++++ +D I  +K ++++KEGIPP QQRLIF GKQ+ D +  ++Y+I+  S LHLVL LRGG+
Sbjct:    1 MLIKVKTLTGKEIEIDIDPTDKIQRIKERVEEKEGIPPSQQRLIFGGKQMGDDKPASEYSIEGGSVLHLVLALRGGL 77

The following BLAST results are available for this feature:

BLAST of EY665137 vs. ExPASy Swiss-Prot

BLAST of EY665137 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 99


Match Name	E-value	Identity	Description
NEDD8_CAEEL	1.401e-19	60.53	NEDD8 OS=Caenorhabditis elegans GN=ned-8 PE=1 SV=1	[more]
RUB2_ARATH	1.829e-19	61.33	NEDD8-like protein RUB2 OS=Arabidopsis thaliana GN...	[more]
NEDD8_RAT	2.023e-18	56.58	NEDD8 OS=Rattus norvegicus GN=Nedd8 PE=1 SV=1	[more]
NEDD8_RABIT	2.023e-18	56.58	NEDD8 OS=Oryctolagus cuniculus GN=NEDD8 PE=3 SV=1	[more]
NEDD8_MOUSE	2.023e-18	56.58	NEDD8 OS=Mus musculus GN=Nedd8 PE=1 SV=2	[more]
NEDD8_HUMAN	2.023e-18	56.58	NEDD8 OS=Homo sapiens GN=NEDD8 PE=1 SV=1	[more]
NEDD8_BOVIN	2.023e-18	56.58	NEDD8 OS=Bos taurus GN=NEDD8 PE=3 SV=1	[more]
RUB3_ORYSJ	2.642e-18	53.95	NEDD8-like protein RUB3 OS=Oryza sativa subsp. jap...	[more]
RUB3_ARATH	1.712e-17	55.26	NEDD8-like protein RUB3 OS=Arabidopsis thaliana GN...	[more]
NEDD8_DICDI	2.236e-17	53.25	NEDD8 OS=Dictyostelium discoideum GN=nedd8 PE=3 SV...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-101-074-D12-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY665137 ID=EY665137; Name=EY665137; organism=Citrus sinensis; type=EST; length=862bp
CCACGCGTCCGCGGNAAGCAACTCGAAGATGGCCGCACCTTGGCCGATTA
CAACATCCAGAAGGAGTCAACGCTCCACCTCGTGCTCAGGCTCCGTGGCG
GCATGCAGATCTTCGTCAAAACCCTGACCGGGAAGACGATTACCCTCGAG
GTGGAGAGCTCCGACACGATCGATAACGTGAAGGCCAAGATCCAAGACAA
GGAGGGAATCCCTCCCGACCAGCAGAGGCTGATCTTCGCCGGAAAGCAAC
TCGAAGACGGCCGGACCTTGGCCGATTACAACATCCAAAAAGAATCCACC
TTGCATCTTGTCCTCAGGCTCCGTGGTGGCATGCAGATCTTCGTCAAAAC
CCTAACTGGGAAGACGATCACTCTCGAGGTGGAGAGTTCAGATACGATTG
ATAATGTCAAGGCCAAGATCCAAGACAAGGAGGGGATCCCACCAGATCAA
CAAAGATTGATCTTTGCTGGAAAACAATTGGAAGACGGCCGTACGTTGGC
TGACTATAATATCCAGAAAGAGTCAACTCTTCACCTTGTCCTCCGTCTTC
GTGGAGGCATGCAAATTTTTGTCAAGACTTTGACTGGGAAGACTATTACT
CTGGAGGTGGANGAGTCTGATACAATTGATAATGTCAAGGCAAAGATTCA
GGATAAGGAAGGGATTCCACCAGATCAGCAGAGGCTTATCTTCGCTGGGA
AGCAGCTTGAGGATGGCCGTACCCTTGCTGTTATAACATCCAAAAGGATC
CACCTNCATCTCGTCCTCGTCTNCGTGTGGTGAATTTTGAGATATTGGTC
GGTGGATCTGTTTCTTTTGTTACTGGGTATGAATACTGGNTGCTTTTTGT
ACCATGCTCTGT