CX672009 | Citrus Genome Database

Overview


Name	CX672009
Unique Name	CX672009
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	833

Libraries


Library Name	Type
Madame Vinous Sweet Orange Multiple Pathogen-Infected cDNA Library UCRCS10	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_LISW6 (tRNA-specific 2-thiouridylase mnmA OS=Listeria welshimeri serovar 6b (strain ATCC 35897 / DSM 20650 / SLCC5334) GN=mnmA PE=3 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 6.657e-19
Identity = 38/83 (45.78%), Postives = 49/83 (59.04%), Query Frame = 1

Query:  274 CPLEEDMKYAKAVYDQVDVPL-EVHLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYANV 519
            C   ED      V +Q+ +P   V+   EYW  V +Y +EEY   RTPNPDV+CN  IKF  F++   S+  DYVA+GHYA V
Sbjct:   51 CTATEDYDDVIRVANQIGIPYYAVNFEKEYWDKVFTYFLEEYKLGRTPNPDVMCNKEIKFKAFLEHAESLGADYVATGHYAQV 133

HSP 2 Score: 36.5798 bits (83), Expect = 6.657e-19
Identity = 18/44 (40.91%), Postives = 26/44 (59.09%), Query Frame = 2

Query:  140 LKVAVLLSGGVDSSVALRLLNAAGHSCIAFYLKIWFQTSRTFGL 271
            ++V V +SGGVDSSV   +L   G+  I  ++K W  T   FG+
Sbjct:    8 IRVVVGMSGGVDSSVTAHILKEQGYDVIGIFMKNWDDTDE-FGV 50

[Close]

BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_BACA2 (tRNA-specific 2-thiouridylase mnmA OS=Bacillus amyloliquefaciens (strain FZB42) GN=mnmA PE=3 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 6.657e-19
Identity = 37/87 (42.53%), Postives = 52/87 (59.77%), Query Frame = 1

Query:  274 CPLEEDMKYAKAVYDQVDVPL-EVHLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYANVVHSS 531
            C   ED +    V +Q+ +P   V+   +YW+ V  Y ++EY   RTPNPDV+CN  IKF  F+D   S+  DY+A+GHYA V  S+
Sbjct:   51 CTATEDYEDVIRVCNQIGIPYYAVNFEKQYWEKVFQYFLDEYKAGRTPNPDVMCNKEIKFKAFLDHALSLGADYLATGHYARVDRSA 137

HSP 2 Score: 36.965 bits (84), Expect = 6.657e-19
Identity = 18/37 (48.65%), Postives = 23/37 (62.16%), Query Frame = 2

Query:  143 KVAVLLSGGVDSSVALRLLNAAGHSCIAFYLKIWFQT 253
            +V V +SGGVDSSVA  LL   G+  I  ++K W  T
Sbjct:    9 RVVVGMSGGVDSSVAALLLKEQGYDVIGIFMKNWDDT 45

[Close]

BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA2_GEOKA (tRNA-specific 2-thiouridylase mnmA 2 OS=Geobacillus kaustophilus GN=mnmA2 PE=3 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 6.657e-19
Identity = 36/91 (39.56%), Postives = 52/91 (57.14%), Query Frame = 1

Query:  274 CPLEEDMKYAKAVYDQVDVPL-EVHLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYANVVHSSADHK 543
            C   ED +    V +Q+ +P   V+   +YW  V +Y + EY   RTPNPDV+CN  IKF  F++   S+  DY+A+GHYA V     ++K
Sbjct:   51 CTATEDYEDVVRVCNQIGIPYYAVNFEKQYWDKVFTYFLNEYKAGRTPNPDVMCNKEIKFKAFLEHAMSIGADYIATGHYARVEFRDGEYK 141

HSP 2 Score: 37.3502 bits (85), Expect = 6.657e-19
Identity = 20/43 (46.51%), Postives = 25/43 (58.14%), Query Frame = 2

Query:  125 PHRKGLKVAVLLSGGVDSSVALRLLNAAGHSCIAFYLKIWFQT 253
            PH    +V V +SGGVDSSVA  LL   G+  I  ++K W  T
Sbjct:    5 PHET--RVVVGMSGGVDSSVAALLLKEQGYDVIGIFMKNWDDT 45

[Close]

BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_NITMU (tRNA-specific 2-thiouridylase mnmA OS=Nitrosospira multiformis (strain ATCC 25196 / NCIMB 11849) GN=mnmA PE=3 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 6.660e-19
Identity = 41/95 (43.16%), Postives = 60/95 (63.16%), Query Frame = 1

Query:  244 VSNFENFWTE--CPLEEDMKYAKAVYDQVDVPLE-VHLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYANV 519
            + N+E+  T+  C   +D+  A +V D + +PLE V+ + EY + V S+ + EY   RTPNPDVLCN  IKF  F+D  + +  DY+A+GHYA V
Sbjct:   35 MKNWEDDDTDEYCSSRQDLIDAASVADVIGIPLEAVNFSAEYKERVFSHFLAEYKAGRTPNPDVLCNAEIKFKAFLDHAAGLGADYIATGHYAQV 129

HSP 2 Score: 36.1946 bits (82), Expect = 6.660e-19
Identity = 17/34 (50.00%), Postives = 22/34 (64.71%), Query Frame = 2

Query:  143 KVAVLLSGGVDSSVALRLLNAAGHSCIAFYLKIW 244
            +V V +SGGVDSSVA  LL   G+  I  ++K W
Sbjct:    5 RVVVGMSGGVDSSVAALLLKQQGYEVIGLFMKNW 38

[Close]

BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_FRATO (tRNA-specific 2-thiouridylase mnmA OS=Francisella tularensis subsp. holarctica (strain OSU18) GN=mnmA PE=3 SV=2)

HSP 1 Score: 81.6481 bits (200), Expect = 6.662e-19
Identity = 36/81 (44.44%), Postives = 52/81 (64.20%), Query Frame = 1

Query:  274 CPLEEDMKYAKAVYDQVDVPLE-VHLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYA 513
            C  E+D+  A+AV D + +P + ++ T EYW NV  + + EY   RTPNPD+LCN  IKF  F+  +  +  DY+A+GHYA
Sbjct:   47 CSAEQDIADAQAVCDSIGIPFKKINFTAEYWDNVFEHFLIEYKAGRTPNPDILCNKEIKFKAFLSYVHLLGGDYIATGHYA 127

HSP 2 Score: 33.4982 bits (75), Expect = 6.662e-19
Identity = 16/34 (47.06%), Postives = 21/34 (61.76%), Query Frame = 2

Query:  143 KVAVLLSGGVDSSVALRLLNAAGHSCIAFYLKIW 244
            KV V +SGGVDSSV+  LL   G+     ++K W
Sbjct:    5 KVIVGISGGVDSSVSALLLKQQGYDVTGVFMKNW 38

[Close]

BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_FRATH (tRNA-specific 2-thiouridylase mnmA OS=Francisella tularensis subsp. holarctica (strain LVS) GN=mnmA PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 6.662e-19
Identity = 36/81 (44.44%), Postives = 52/81 (64.20%), Query Frame = 1

Query:  274 CPLEEDMKYAKAVYDQVDVPLE-VHLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYA 513
            C  E+D+  A+AV D + +P + ++ T EYW NV  + + EY   RTPNPD+LCN  IKF  F+  +  +  DY+A+GHYA
Sbjct:   47 CSAEQDIADAQAVCDSIGIPFKKINFTAEYWDNVFEHFLIEYKAGRTPNPDILCNKEIKFKAFLSYVHLLGGDYIATGHYA 127

HSP 2 Score: 33.4982 bits (75), Expect = 6.662e-19
Identity = 16/34 (47.06%), Postives = 21/34 (61.76%), Query Frame = 2

Query:  143 KVAVLLSGGVDSSVALRLLNAAGHSCIAFYLKIW 244
            KV V +SGGVDSSV+  LL   G+     ++K W
Sbjct:    5 KVIVGISGGVDSSVSALLLKQQGYDVTGVFMKNW 38

[Close]

BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_FRATF (tRNA-specific 2-thiouridylase mnmA OS=Francisella tularensis subsp. holarctica (strain FTNF002-00 / FTA) GN=mnmA PE=3 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 6.662e-19
Identity = 36/81 (44.44%), Postives = 52/81 (64.20%), Query Frame = 1

Query:  274 CPLEEDMKYAKAVYDQVDVPLE-VHLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYA 513
            C  E+D+  A+AV D + +P + ++ T EYW NV  + + EY   RTPNPD+LCN  IKF  F+  +  +  DY+A+GHYA
Sbjct:   47 CSAEQDIADAQAVCDSIGIPFKKINFTAEYWDNVFEHFLIEYKAGRTPNPDILCNKEIKFKAFLSYVHLLGGDYIATGHYA 127

HSP 2 Score: 33.4982 bits (75), Expect = 6.662e-19
Identity = 16/34 (47.06%), Postives = 21/34 (61.76%), Query Frame = 2

Query:  143 KVAVLLSGGVDSSVALRLLNAAGHSCIAFYLKIW 244
            KV V +SGGVDSSV+  LL   G+     ++K W
Sbjct:    5 KVIVGISGGVDSSVSALLLKQQGYDVTGVFMKNW 38

[Close]

BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_PEDPA (tRNA-specific 2-thiouridylase mnmA OS=Pediococcus pentosaceus (strain ATCC 25745 / 183-1w) GN=mnmA PE=3 SV=1)

HSP 1 Score: 80.1073 bits (196), Expect = 8.653e-19
Identity = 39/91 (42.86%), Postives = 51/91 (56.04%), Query Frame = 1

Query:  274 CPLEEDMKYAKAVYDQVDVPL-EVHLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYANVVHSSADHK 543
            C   ED K    V  ++ +P   V+   EYW  V +Y ++EY   RTPNPDV+CN  IKF  F+D   S+  DYVA+GHYA V      H+
Sbjct:   50 CTATEDYKDVAKVAAKIGIPYYSVNFEKEYWDRVFTYFLDEYKKGRTPNPDVICNNEIKFKAFLDYAISLGADYVATGHYAQVERDENGHQ 140

HSP 2 Score: 34.6538 bits (78), Expect = 8.653e-19
Identity = 16/37 (43.24%), Postives = 22/37 (59.46%), Query Frame = 2

Query:  143 KVAVLLSGGVDSSVALRLLNAAGHSCIAFYLKIWFQT 253
            +V V +SGGVDSSV   LL   G+  +  ++K W  T
Sbjct:    8 RVVVGMSGGVDSSVVAYLLKQQGYDVVGVFMKNWDDT 44

[Close]

BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_BACSK (tRNA-specific 2-thiouridylase mnmA OS=Bacillus clausii (strain KSM-K16) GN=mnmA PE=3 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 8.660e-19
Identity = 36/91 (39.56%), Postives = 53/91 (58.24%), Query Frame = 1

Query:  274 CPLEEDMKYAKAVYDQVDVPL-EVHLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYANVVHSSADHK 543
            C   ED +  + V  Q+ +P   V+   +YW  V +Y +EEY   RTPNPDV+CN  IKF  F++   S+  DYVA+GHYA +     +++
Sbjct:   50 CSATEDYEDVERVAQQLGIPYYAVNFEKQYWDKVFTYFLEEYKAGRTPNPDVMCNKEIKFKAFLNHAISLGADYVATGHYAQLQELDGEYR 140

HSP 2 Score: 37.3502 bits (85), Expect = 8.660e-19
Identity = 18/41 (43.90%), Postives = 24/41 (58.54%), Query Frame = 2

Query:  122 MPHRKGLKVAVLLSGGVDSSVALRLLNAAGHSCIAFYLKIW 244
            M  R+  +V V +SGGVDSSV   LL   G+  I  ++K W
Sbjct:    1 MKKREDTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNW 41

[Close]

BLAST of CX672009 vs. ExPASy Swiss-Prot
Match: MNMA_LACGA (tRNA-specific 2-thiouridylase mnmA OS=Lactobacillus gasseri (strain ATCC 33323 / DSM 20243) GN=mnmA PE=3 SV=2)

HSP 1 Score: 79.7221 bits (195), Expect = 1.125e-18
Identity = 41/111 (36.94%), Postives = 60/111 (54.05%), Query Frame = 1

Query:  274 CPLEEDMKYAKAVYDQVDVPL-EVHLTDEYWKNVVSYIIEEY*CDRTPNPDVLCNTRIKFGVFMDAISSMEFDYVASGHYA--NVVHSSADHKDKPSV*NYQKTSCLSWIN 597
            C   ED +  K V D++ +P   ++   +YW+ V  Y + EY   RTPNPDV+CN  IKF  F+D    ++ DY+A GHYA   V  +   H  +P   N  +T  LS ++
Sbjct:   50 CTATEDYEDVKKVADEIGIPYYSINFEKDYWERVFEYFLNEYKKGRTPNPDVMCNKEIKFKSFLDFAMDLDADYIAMGHYAATKVDENGIVHMMRPKDGNKDQTYFLSQLS 160

HSP 2 Score: 34.6538 bits (78), Expect = 1.125e-18
Identity = 16/38 (42.11%), Postives = 23/38 (60.53%), Query Frame = 2

Query:  140 LKVAVLLSGGVDSSVALRLLNAAGHSCIAFYLKIWFQT 253
            ++V V +SGGVDSSV+  LL   G   +  ++K W  T
Sbjct:    7 IRVVVGMSGGVDSSVSALLLKQQGFDVVGVFMKNWDDT 44

The following BLAST results are available for this feature:

BLAST of CX672009 vs. ExPASy Swiss-Prot

BLAST of CX672009 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 471


Match Name	E-value	Identity	Description
MNMA_LISW6	6.657e-19	45.78	tRNA-specific 2-thiouridylase mnmA OS=Listeria wel...	[more]
MNMA_BACA2	6.657e-19	42.53	tRNA-specific 2-thiouridylase mnmA OS=Bacillus amy...	[more]
MNMA2_GEOKA	6.657e-19	39.56	tRNA-specific 2-thiouridylase mnmA 2 OS=Geobacillu...	[more]
MNMA_NITMU	6.660e-19	43.16	tRNA-specific 2-thiouridylase mnmA OS=Nitrosospira...	[more]
MNMA_FRATO	6.662e-19	44.44	tRNA-specific 2-thiouridylase mnmA OS=Francisella ...	[more]
MNMA_FRATH	6.662e-19	44.44	tRNA-specific 2-thiouridylase mnmA OS=Francisella ...	[more]
MNMA_FRATF	6.662e-19	44.44	tRNA-specific 2-thiouridylase mnmA OS=Francisella ...	[more]
MNMA_PEDPA	8.653e-19	42.86	tRNA-specific 2-thiouridylase mnmA OS=Pediococcus ...	[more]
MNMA_BACSK	8.660e-19	39.56	tRNA-specific 2-thiouridylase mnmA OS=Bacillus cla...	[more]
MNMA_LACGA	1.125e-18	36.94	tRNA-specific 2-thiouridylase mnmA OS=Lactobacillu...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS10_14D10_g Madame Vinous Sweet Orange Multiple Pathogen-Infected cDNA Library UCRCS10 Citrus sinensis cDNA clone UCRCS10-14D10-G20-5.g, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CX672009 ID=CX672009; Name=CX672009; organism=Citrus sinensis; type=EST; length=833bp
CAAAAACTAATCTTCCTTAACTCTTGTTCTCTGTCAACATCAACCCAGCT
CACACTCTTTACAACACAATCCTAATTCGAATGTTAGTGTTGATTTTGAA
CCCCACTATCTCTCTTGCTGCATGCCCCATCGAAAGGGCCTTAAGGTTGC
TGTTCTTTTGAGCGGCGGCGTTGATAGCAGTGTTGCACTTAGACTTCTTA
ATGCTGCTGGTCATTCTTGCATAGCCTTTTACCTCAAAATTTGGTTTCAA
ACTTCGAGAACTTTTGGACTGAATGCCCATTGGAAGAAGATATGAAGTAT
GCAAAAGCTGTTTATGATCAGGTTGATGTACCACTAGAAGTGCATTTAAC
AGATGAATACTGGAAAAATGTGGTATCCTACATTATTGAAGAATATTGAT
GCGACCGTACACCCAACCCAGATGTCCTTTGTAATACAAGAATAAAATTT
GGTGTGTTCATGGATGCCATCAGCAGTATGGAATTTGATTACGTTGCTTC
TGGGCATTATGCCAATGTTGTCCACTCATCTGCGGATCATAAGGATAAGC
CTTCTGTGTAGAATTATCAAAAGACAAGTTGCTTATCCTGGATAAACATT
TGTGCTTTCATATTTCTCTCCTGTATTGGTGACTTGATTGTTTCCATTTG
GCATGTTTTTCTAAGGAATTCTGCTGAAGCTTTTGCTTTTATAAATGCTC
TTATTAGATATTATGACCATTATTCACATAAAAAATAGTGATTGTCACTT
AAATATGAAAAGCCCAGAGGCACCATGAATTCATCCCCAATTTGTTTATC
CTTTTCAAATCTCTTTTATCATAATCTATGGCC