CX671696 | Citrus Genome Database

Overview


Name	CX671696
Unique Name	CX671696
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	797

Libraries


Library Name	Type
Madame Vinous Sweet Orange Multiple Pathogen-Infected cDNA Library UCRCS10	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CX671696 vs. ExPASy Swiss-Prot
Match: CALR_CRIGR (Calreticulin OS=Cricetulus griseus GN=CALR PE=2 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 4.912e-21
Identity = 36/74 (48.65%), Postives = 52/74 (70.27%), Query Frame = 1

Query:  223 QKIKNPNYKGKWKAPMIDNPDFKDDPDLYVYPNLKYVGIELWQVKSGTMFDNVLVSDDPEYANKLAEETWGKHK 444
            ++I NP+YKG W  P IDNP++  D ++Y Y +   +G++LWQVKSGT+FDN L+++D  YA +   ETWG  K
Sbjct:  278 RQIDNPDYKGTWIHPEIDNPEYSPDANIYAYDSFAVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTK 351

HSP 2 Score: 32.3426 bits (72), Expect = 4.912e-21
Identity = 17/48 (35.42%), Postives = 20/48 (41.67%), Query Frame = 2

Query:    5 KPEGYDDIPKEIXXXXXXXXXXXXXXXXXXXXXPTIPNPEYKGPWKPK 148
            KPE +D  P+ I                     P I NPEYKG WKP+
Sbjct:  232 KPEDWDK-PEHIPDPDAKKPEDWDEEMDGEWEPPVIQNPEYKGEWKPR 278

[Close]

BLAST of CX671696 vs. ExPASy Swiss-Prot
Match: CALR_RAT (Calreticulin OS=Rattus norvegicus GN=Calr PE=1 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 4.913e-21
Identity = 36/74 (48.65%), Postives = 52/74 (70.27%), Query Frame = 1

Query:  223 QKIKNPNYKGKWKAPMIDNPDFKDDPDLYVYPNLKYVGIELWQVKSGTMFDNVLVSDDPEYANKLAEETWGKHK 444
            ++I NP+YKG W  P IDNP++  D ++Y Y +   +G++LWQVKSGT+FDN L+++D  YA +   ETWG  K
Sbjct:  278 RQIDNPDYKGTWIHPEIDNPEYSPDANIYAYDSFAVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTK 351

HSP 2 Score: 32.3426 bits (72), Expect = 4.913e-21
Identity = 17/48 (35.42%), Postives = 20/48 (41.67%), Query Frame = 2

Query:    5 KPEGYDDIPKEIXXXXXXXXXXXXXXXXXXXXXPTIPNPEYKGPWKPK 148
            KPE +D  P+ I                     P I NPEYKG WKP+
Sbjct:  232 KPEDWDK-PEHIPDPDAKKPEDWDEEMDGEWEPPVIQNPEYKGEWKPR 278

[Close]

BLAST of CX671696 vs. ExPASy Swiss-Prot
Match: CALR_MOUSE (Calreticulin OS=Mus musculus GN=Calr PE=1 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 4.913e-21
Identity = 36/74 (48.65%), Postives = 52/74 (70.27%), Query Frame = 1

Query:  223 QKIKNPNYKGKWKAPMIDNPDFKDDPDLYVYPNLKYVGIELWQVKSGTMFDNVLVSDDPEYANKLAEETWGKHK 444
            ++I NP+YKG W  P IDNP++  D ++Y Y +   +G++LWQVKSGT+FDN L+++D  YA +   ETWG  K
Sbjct:  278 RQIDNPDYKGTWIHPEIDNPEYSPDANIYAYDSFAVLGLDLWQVKSGTIFDNFLITNDEAYAEEFGNETWGVTK 351

HSP 2 Score: 32.3426 bits (72), Expect = 4.913e-21
Identity = 17/48 (35.42%), Postives = 20/48 (41.67%), Query Frame = 2

Query:    5 KPEGYDDIPKEIXXXXXXXXXXXXXXXXXXXXXPTIPNPEYKGPWKPK 148
            KPE +D  P+ I                     P I NPEYKG WKP+
Sbjct:  232 KPEDWDK-PEHIPDPDAKKPEDWDEEMDGEWEPPVIQNPEYKGEWKPR 278

[Close]

BLAST of CX671696 vs. ExPASy Swiss-Prot
Match: CALR_ONCVO (Calreticulin OS=Onchocerca volvulus GN=crt-1 PE=2 SV=2)

HSP 1 Score: 82.8037 bits (203), Expect = 5.244e-20
Identity = 39/71 (54.93%), Postives = 51/71 (71.83%), Query Frame = 1

Query:  217 PLQKIKNPNYKGKWKAPMIDNPDFKDDPDLYVYPNLKYVGIELWQVKSGTMFDNVLVSDDPEYANKLAEET 429
            P QK KNP YKGKW  P I+ PD+  D +LYVY ++  +G +LWQVKSGT+FD+V+V+D  E A K  E+T
Sbjct:  275 PKQK-KNPAYKGKWIHPEIEIPDYTPDDNLYVYDDIGAIGFDLWQVKSGTIFDDVIVTDSVEEAKKFGEKT 344

HSP 2 Score: 35.4242 bits (80), Expect = 5.244e-20
Identity = 19/52 (36.54%), Postives = 22/52 (42.31%), Query Frame = 2

Query:    2 KKPEGYDDIPKEIXXXXXXXXXXXXXXXXXXXXXPTIPNPEYKGPWKPKVTK 157
            KKPE +D  P+ I                     P + NPEYKG WKPK  K
Sbjct:  229 KKPEDWDK-PEHIPDPDAKKPEDWDDEMDGEWEPPMVDNPEYKGEWKPKQKK 279

[Close]

BLAST of CX671696 vs. ExPASy Swiss-Prot
Match: CALR_CAEEL (Calreticulin OS=Caenorhabditis elegans GN=crt-1 PE=1 SV=1)

HSP 1 Score: 84.7297 bits (208), Expect = 6.818e-20
Identity = 38/76 (50.00%), Postives = 53/76 (69.74%), Query Frame = 1

Query:  223 QKIKNPNYKGKWKAPMIDNPDFKDDPDLYVYPNLKYVGIELWQVKSGTMFDNVLVSDDPEYANKLAEETWGKHKDV 450
            ++IKNP YKGKW  P I+NP++  D +LY Y +   +G +LWQVKSGT+FDN++++D  E A   A ET+ K K V
Sbjct:  273 KQIKNPAYKGKWIHPEIENPEYTPDDELYSYESWGAIGFDLWQVKSGTIFDNIIITDSVEEAEAHAAETFDKLKTV 348

HSP 2 Score: 33.113 bits (74), Expect = 6.818e-20
Identity = 13/18 (72.22%), Postives = 13/18 (72.22%), Query Frame = 2

Query:  104 PTIPNPEYKGPWKPKVTK 157
            P I NPEYKG WKPK  K
Sbjct:  259 PMIDNPEYKGEWKPKQIK 276

[Close]

BLAST of CX671696 vs. ExPASy Swiss-Prot
Match: CALR_DROME (Calreticulin OS=Drosophila melanogaster GN=Crc PE=1 SV=2)

HSP 1 Score: 78.9518 bits (193), Expect = 7.704e-18
Identity = 35/68 (51.47%), Postives = 48/68 (70.59%), Query Frame = 1

Query:  223 QKIKNPNYKGKWKAPMIDNPDFKDDPDLYVYPNLKYVGIELWQVKSGTMFDNVLVSDDPEYANKLAEE 426
            +++ NPNYKG W+ P I NP++  D  LY+   +  +G +LWQVKSGT+FDNVL++DD E A K A E
Sbjct:  278 KQLDNPNYKGAWEHPEIANPEYVPDDKLYLRKEICTLGFDLWQVKSGTIFDNVLITDDVELAAKAAAE 345

HSP 2 Score: 31.9574 bits (71), Expect = 7.704e-18
Identity = 17/49 (34.69%), Postives = 21/49 (42.86%), Query Frame = 2

Query:    2 KKPEGYDDIPKEIXXXXXXXXXXXXXXXXXXXXXPTIPNPEYKGPWKPK 148
            KKPE +D  P+ I                     P I NPE+KG W+PK
Sbjct:  231 KKPEDWDK-PEHIPDPDATKPEDWDDEMDGEWEPPMIDNPEFKGEWQPK 278

[Close]

BLAST of CX671696 vs. ExPASy Swiss-Prot
Match: CALR_EUGGR (Calreticulin OS=Euglena gracilis PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 3.721e-17
Identity = 33/75 (44.00%), Postives = 49/75 (65.33%), Query Frame = 1

Query:  223 QKIKNPNYKGKWKAPMIDNPDFKDDPDLYVYPNLKYVGIELWQVKSGTMFDNVLVSDDPEYANKLAEETWGKHKD 447
            +KI NP YKG WK   I NP+++ D  ++++  +  VG +LWQVKSGT+FDN++V+D    A    ++T G  KD
Sbjct:  275 KKIPNPAYKGVWKPRDIPNPEYEADDKVHIFDEIAAVGFDLWQVKSGTIFDNIIVTDSLAEAKAFYDQTNGATKD 349

HSP 2 Score: 33.8834 bits (76), Expect = 3.721e-17
Identity = 17/49 (34.69%), Postives = 20/49 (40.82%), Query Frame = 2

Query:    2 KKPEGYDDIPKEIXXXXXXXXXXXXXXXXXXXXXPTIPNPEYKGPWKPK 148
            KKPE +D  P +I                     P I NP+YKG WK K
Sbjct:  227 KKPEDWDKEPAQIPDPDATQPDDWDEEEDGKWEAPMISNPKYKGEWKAK 275

[Close]

BLAST of CX671696 vs. ExPASy Swiss-Prot
Match: CALR_BOMMO (Calreticulin OS=Bombyx mori GN=crt PE=1 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 1.796e-16
Identity = 33/67 (49.25%), Postives = 46/67 (68.66%), Query Frame = 1

Query:  223 QKIKNPNYKGKWKAPMIDNPDFKDDPDLYVYPNLKYVGIELWQVKSGTMFDNVLVSDDPEYANKLAE 423
            ++I NP YKG W  P IDNP++  D +LY    +  VG++LWQVKSGT+FD+ L++DDP  A +  E
Sbjct:  278 KQIDNPAYKGPWVHPEIDNPEYTPDSNLYKRDEICAVGLDLWQVKSGTIFDDFLITDDPAAAKERGE 344

HSP 2 Score: 31.187 bits (69), Expect = 1.796e-16
Identity = 17/49 (34.69%), Postives = 20/49 (40.82%), Query Frame = 2

Query:    2 KKPEGYDDIPKEIXXXXXXXXXXXXXXXXXXXXXPTIPNPEYKGPWKPK 148
            KKPE +D  P+ I                     P I NP+YKG W PK
Sbjct:  231 KKPEDWDK-PEHIPDPDATKPEDWDDEMDGEWEPPMIDNPDYKGVWAPK 278

[Close]

BLAST of CX671696 vs. ExPASy Swiss-Prot
Match: CALX_SOYBN (Calnexin homolog OS=Glycine max PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 1.370e-11
Identity = 32/80 (40.00%), Postives = 44/80 (55.00%), Query Frame = 1

Query:  232 KNPNYKGKWKAPMIDNPDFKDDPDLYVYPNLKY-------------VGIELWQVKSGTMFDNVLVSDDPEYANKLAEETW 432
            +NP YKGKW AP IDNP +K        PN +Y             +GIE+W ++ G +FDNVL+++D + A    E TW
Sbjct:  331 RNPAYKGKWSAPYIDNPSYKGIWKPREIPNPEYFELAKPDFEPIAAIGIEIWTMQDGILFDNVLIANDDKVAESYRETTW 410

HSP 2 Score: 22.7126 bits (47), Expect = 1.370e-11
Identity = 8/12 (66.67%), Postives = 8/12 (66.67%), Query Frame = 2

Query:  104 PTIPNPEYKGPW 139
            PT  NP YKG W
Sbjct:  328 PTKRNPAYKGKW 339

[Close]

BLAST of CX671696 vs. ExPASy Swiss-Prot
Match: CALX_PEA (Calnexin homolog OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.779e-11
Identity = 32/79 (40.51%), Postives = 43/79 (54.43%), Query Frame = 1

Query:  235 NPNYKGKWKAPMIDNPDFKDDPDLYVYPNLKY-------------VGIELWQVKSGTMFDNVLVSDDPEYANKLAEETW 432
            NP YKGKW AP IDNP++K        PN +Y             +GIE+W ++ G +FDNVL++ D + A    E TW
Sbjct:  331 NPAYKGKWSAPYIDNPNYKGIWKPQEIPNPEYFELEKPDFEPIAAIGIEIWTMQDGILFDNVLIAKDDKIAESYRETTW 409

HSP 2 Score: 23.0978 bits (48), Expect = 1.779e-11
Identity = 8/12 (66.67%), Postives = 8/12 (66.67%), Query Frame = 2

Query:  104 PTIPNPEYKGPW 139
            PT  NP YKG W
Sbjct:  327 PTKSNPAYKGKW 338

The following BLAST results are available for this feature:

BLAST of CX671696 vs. ExPASy Swiss-Prot

BLAST of CX671696 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 34


Match Name	E-value	Identity	Description
CALR_CRIGR	4.912e-21	48.65	Calreticulin OS=Cricetulus griseus GN=CALR PE=2 SV...	[more]
CALR_RAT	4.913e-21	48.65	Calreticulin OS=Rattus norvegicus GN=Calr PE=1 SV=...	[more]
CALR_MOUSE	4.913e-21	48.65	Calreticulin OS=Mus musculus GN=Calr PE=1 SV=1	[more]
CALR_ONCVO	5.244e-20	54.93	Calreticulin OS=Onchocerca volvulus GN=crt-1 PE=2 ...	[more]
CALR_CAEEL	6.818e-20	50.00	Calreticulin OS=Caenorhabditis elegans GN=crt-1 PE...	[more]
CALR_DROME	7.704e-18	51.47	Calreticulin OS=Drosophila melanogaster GN=Crc PE=...	[more]
CALR_EUGGR	3.721e-17	44.00	Calreticulin OS=Euglena gracilis PE=2 SV=1	[more]
CALR_BOMMO	1.796e-16	49.25	Calreticulin OS=Bombyx mori GN=crt PE=1 SV=1	[more]
CALX_SOYBN	1.370e-11	40.00	Calnexin homolog OS=Glycine max PE=2 SV=1	[more]
CALX_PEA	1.779e-11	40.51	Calnexin homolog OS=Pisum sativum PE=2 SV=1	[more]

Pages

Properties


Property Name	Value
Genbank description	UCRCS10_12F03_g Madame Vinous Sweet Orange Multiple Pathogen-Infected cDNA Library UCRCS10 Citrus sinensis cDNA clone UCRCS10-12F03-L6-5.g, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CX671696 ID=CX671696; Name=CX671696; organism=Citrus sinensis; type=EST; length=797bp
CAAGAAGCCAGAGGGCTATGATGACATTCCAAAGGAGATAACAGATCCTG
ATGCCAAGAAGCCTGATGATTGGGATGATGAGGAAGATGGTGAATGGACA
GCTCCAACCATCCCTAACCCAGAGTACAAGGGTCCATGGAAGCCAAAGGT
TACTAAAATTCTAGATTAATTTGAACCATGTAAATATGGTTTTGGCTATC
TTGTAACTGTGTTTTGCCTCTGCAGAAAATTAAGAACCCTAATTACAAGG
GCAAGTGGAAGGCTCCAATGATTGACAACCCAGATTTCAAGGATGACCCA
GACCTTTATGTGTATCCCAACTTGAAGTATGTTGGCATTGAGTTGTGGCA
GGTGAAATCTGGAACCATGTTTGACAATGTCTTGGTTAGTGATGATCCAG
AGTATGCCAATAAGTTGGCCGAAGAAACATGGGGAAAGCATAAGGATGTA
TGATTGTTTTCTCCTCATCAAATCTGTCTCATAACTGATACTTACAAGCC
TCAGTATTTGATTTGGAGACCTATTGTTTTTGCAGGCCGAGAAGGCAGCA
TTTGATGAGGCCGAGAAGAAGAGAGAGGAGGAGGTGCTTTTTTGCTCAAT
TACTTTGTTGCACATTTTATTTTTTCTTTGGAATTGGGTTTTTAGCATAC
TTAGCTGAGTTTCCAAAGACATAATCATTTACCTAGATGAAAACTTATCT
GTTGAAAACTTTTTTGATTCTATTCTGTGTTACCAGTTATGCTAACTTTC
TGAAATGTGGTTAACAGGAATCAAAGGACGCACCTGATTCTGATGCT