CV712848 | Citrus Genome Database

Overview


Name	CV712848
Unique Name	CV712848
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	834

Libraries


Library Name	Type
Parent Washington Navel Orange Callus cDNA Library UCRCS08-1	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CV712848 vs. ExPASy Swiss-Prot
Match: ISPH_MICAN (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Microcystis aeruginosa (strain NIES-843) GN=ispH PE=3 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 3.666e-20
Identity = 35/68 (51.47%), Postives = 52/68 (76.47%), Query Frame = -1

Query:  289 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGELV 492
            QERQDA++ LV+E + L++++G +NSS T+ LQ+IA +R I SY IDS +RI PGN+I HK +  +L+
Sbjct:  292 QERQDAIFELVKEPLSLMVVIGGYNSSNTTHLQEIAIERAIPSYHIDSAERILPGNRIEHKPLGDDLI 359

HSP 2 Score: 47.3654 bits (111), Expect = 3.666e-20
Identity = 19/31 (61.29%), Postives = 26/31 (83.87%), Query Frame = -3

Query:  188 NWLPKGQITIGVTAGASIPDKAVENVLKKVF 280
            NWL +G+I +GVT+GAS PDK VE V++K+F
Sbjct:  363 NWLNEGKIVVGVTSGASTPDKVVEEVIEKIF 393

[Close]

BLAST of CV712848 vs. ExPASy Swiss-Prot
Match: ISPH_SYNP2 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain ATCC 27264 / PCC 7002 / PR-6) GN=ispH PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 6.201e-20
Identity = 36/67 (53.73%), Postives = 50/67 (74.63%), Query Frame = -1

Query:  292 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGEL 492
            QERQDAM+ LVE+ +DL++++G +NSS T+ LQ+IA +R I SY IDS  R+   N IAHK + GE+
Sbjct:  292 QERQDAMFDLVEKDLDLMVVIGGFNSSNTTHLQEIAVERNIPSYHIDSGDRLLGNNVIAHKPLDGEI 358

HSP 2 Score: 47.7506 bits (112), Expect = 6.201e-20
Identity = 20/40 (50.00%), Postives = 30/40 (75.00%), Query Frame = -3

Query:  167 EKNWLPKGQITIGVTAGASIPDKAVENVLKKVFEIEREEA 286
            + +WLP G++ IGVT+GAS PDK VE+V+ K+F  + + A
Sbjct:  361 QTHWLPAGKLKIGVTSGASTPDKVVEDVIAKIFAEKAQPA 400

[Close]

BLAST of CV712848 vs. ExPASy Swiss-Prot
Match: ISPH_PROM4 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Prochlorococcus marinus (strain MIT 9211) GN=ispH PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 6.201e-20
Identity = 35/72 (48.61%), Postives = 53/72 (73.61%), Query Frame = -1

Query:  280 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIG-PGNKIAHKLMHGELVEKK 492
            +ERQDAM+ LV+E +DL++++G +NSS T+ LQ+IA  RGI S+ ID+ +RIG   N I HK +  +L+ +K
Sbjct:  292 EERQDAMFSLVDEPLDLLVVIGGFNSSNTTHLQEIAISRGIRSFHIDTPERIGDQENTITHKPLGDDLITEK 363

HSP 2 Score: 50.447 bits (119), Expect = 6.201e-20
Identity = 20/40 (50.00%), Postives = 32/40 (80.00%), Query Frame = -3

Query:  167 EKNWLPKGQITIGVTAGASIPDKAVENVLKKVFEIEREEA 286
            EKN+LP+G I++G+T+GAS PD+ VE+V+ K+  +  E+A
Sbjct:  362 EKNFLPEGNISVGITSGASTPDRVVEHVIHKLINLSEEKA 401

[Close]

BLAST of CV712848 vs. ExPASy Swiss-Prot
Match: ISPH_GLOVI (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Gloeobacter violaceus GN=ispH PE=3 SV=1)

HSP 1 Score: 65.4698 bits (158), Expect = 8.059e-20
Identity = 31/66 (46.97%), Postives = 47/66 (71.21%), Query Frame = -1

Query:  295 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGE 492
            QERQDAM+ LVEE +DL++++G +NSS T+ LQ+IA +RG+ S+ ID   R+     I H+ +H +
Sbjct:  286 QERQDAMFKLVEEPLDLLVVIGGYNSSNTTHLQEIAVERGLPSFHIDGADRLVSAQFIEHRDLHSK 351

HSP 2 Score: 52.7582 bits (125), Expect = 8.059e-20
Identity = 20/35 (57.14%), Postives = 29/35 (82.86%), Query Frame = -3

Query:  179 KNWLPKGQITIGVTAGASIPDKAVENVLKKVFEIE 283
            KNWLP G++T+GVTAGAS PD+ V  V+ ++FE++
Sbjct:  357 KNWLPAGEVTVGVTAGASTPDRVVAEVIARIFELK 391

[Close]

BLAST of CV712848 vs. ExPASy Swiss-Prot
Match: ISPH_CYAP7 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC 7424) GN=ispH PE=3 SV=1)

HSP 1 Score: 66.2402 bits (160), Expect = 1.774e-19
Identity = 36/71 (50.70%), Postives = 49/71 (69.01%), Query Frame = -1

Query:  280 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGELVEKK 492
            QERQDAM  LV+EK+DL++++G +NSS T+ LQ+I+   GI SY IDS  RI   N I HK +  +L  K+
Sbjct:  292 QERQDAMLDLVKEKLDLMVVIGGFNSSNTTHLQEISIHHGIVSYHIDSAARIDLNNHIEHKPLGKDLEVKE 362

HSP 2 Score: 50.8322 bits (120), Expect = 1.774e-19
Identity = 22/41 (53.66%), Postives = 31/41 (75.61%), Query Frame = -3

Query:  164 EKNWLPKGQITIGVTAGASIPDKAVENVLKKVFEIEREEAL 286
            ++NWLP G+I +GVT+GAS PD  VE VL+K+F I+   A+
Sbjct:  361 KENWLPDGKIVVGVTSGASTPDIVVEQVLQKIFAIKSAVAM 401

[Close]

BLAST of CV712848 vs. ExPASy Swiss-Prot
Match: ISPH_SYNPW (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain WH7803) GN=ispH PE=3 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 6.601e-19
Identity = 35/70 (50.00%), Postives = 52/70 (74.29%), Query Frame = -1

Query:  283 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGELVEK 492
            QERQDAM+ LV+E +DL++++G +NSS T+ LQ+IA +RGI S+ ID+ +RI   N I HK +  EL+ +
Sbjct:  292 QERQDAMFSLVDEPLDLMVVIGGYNSSNTTHLQEIAINRGIRSFHIDTPERISADNSIEHKPLGQELIRE 361

HSP 2 Score: 43.5134 bits (101), Expect = 6.601e-19
Identity = 16/38 (42.11%), Postives = 28/38 (73.68%), Query Frame = -3

Query:  173 EKNWLPKGQITIGVTAGASIPDKAVENVLKKVFEIERE 286
            E  +LP G +T+G+T+GAS PD+ VE+V++++  +  E
Sbjct:  361 ELEFLPSGTVTVGITSGASTPDRVVEHVIQRLISLSEE 398

[Close]

BLAST of CV712848 vs. ExPASy Swiss-Prot
Match: ISPH_CYAP8 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Cyanothece sp. (strain PCC 8801) GN=ispH PE=3 SV=1)

HSP 1 Score: 67.3958 bits (163), Expect = 8.581e-19
Identity = 36/71 (50.70%), Postives = 51/71 (71.83%), Query Frame = -1

Query:  280 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGELVEKK 492
            QERQDAM  LV+EK+DL++++G +NSS T+ LQ+I   +GI+SY ID+  RI P N I HK +  +L  K+
Sbjct:  292 QERQDAMLELVKEKLDLMVVIGGFNSSNTTHLQEIPIYQGITSYHIDNATRIKPNNCIEHKPLGKDLEIKE 362

HSP 2 Score: 47.3654 bits (111), Expect = 8.581e-19
Identity = 19/39 (48.72%), Postives = 29/39 (74.36%), Query Frame = -3

Query:  170 EKNWLPKGQITIGVTAGASIPDKAVENVLKKVFEIEREE 286
            ++NWLP+G I +G+T+GAS PD  VE V++K+F  +  E
Sbjct:  361 KENWLPEGSIVVGITSGASTPDIIVEAVIEKIFSAKELE 399

[Close]

BLAST of CV712848 vs. ExPASy Swiss-Prot
Match: ISPH_SYNR3 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Synechococcus sp. (strain RCC307) GN=ispH PE=3 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 1.451e-18
Identity = 36/67 (53.73%), Postives = 51/67 (76.12%), Query Frame = -1

Query:  292 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGEL 492
            QERQDAM+ LV+E +DL++++G +NSS T+ LQ+IA  RGI S  ID+ +RIGPGN + HK +  +L
Sbjct:  292 QERQDAMFSLVDEPLDLMVVIGGYNSSNTTHLQEIAVSRGIPSVHIDAPERIGPGNAVEHKPLGQDL 358

HSP 2 Score: 39.2762 bits (90), Expect = 1.451e-18
Identity = 18/41 (43.90%), Postives = 30/41 (73.17%), Query Frame = -3

Query:  155 WLPKGQITIGVTAGASIPDKAVENVLKKVFEIEREEALQLA 277
            +LP+G + IG+T+GAS PD+ VE V+ ++ ++   EA Q+A
Sbjct:  364 FLPEGDLRIGITSGASTPDRVVEGVIDRLLQL--AEAGQIA 402

[Close]

BLAST of CV712848 vs. ExPASy Swiss-Prot
Match: ISPH_PROMM (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Prochlorococcus marinus (strain MIT 9313) GN=ispH PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 3.191e-18
Identity = 33/67 (49.25%), Postives = 49/67 (73.13%), Query Frame = -1

Query:  292 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGEL 492
            +ERQ AM+ LV+E +DL++++G +NSS T+ LQ+IA  RGI S+ ID+ +RIG  N I HK +  +L
Sbjct:  292 EERQQAMFSLVDEPLDLLVVIGGFNSSNTTHLQEIAISRGIRSFHIDTPERIGDNNSIQHKPLGEDL 358

HSP 2 Score: 45.0542 bits (105), Expect = 3.191e-18
Identity = 16/35 (45.71%), Postives = 29/35 (82.86%), Query Frame = -3

Query:  182 EKNWLPKGQITIGVTAGASIPDKAVENVLKKVFEI 286
            E N+LP G +++G+T+GAS PD+ VE+V++K+ ++
Sbjct:  361 ESNFLPAGSVSVGITSGASTPDRVVEHVIQKLIDL 395

[Close]

BLAST of CV712848 vs. ExPASy Swiss-Prot
Match: ISPH_PROM3 (4-hydroxy-3-methylbut-2-enyl diphosphate reductase OS=Prochlorococcus marinus (strain MIT 9303) GN=ispH PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 4.149e-18
Identity = 33/67 (49.25%), Postives = 49/67 (73.13%), Query Frame = -1

Query:  292 QERQDAMY*LVEEKIDLILLVGRWNSSTTSSLQKIAEDRGISSYWIDSEKRIGPGNKIAHKLMHGEL 492
            +ERQ AM+ LV+E +DL++++G +NSS T+ LQ+IA  RGI S+ ID+ +RIG  N I HK +  +L
Sbjct:  292 EERQQAMFSLVDEPLDLLVVIGGFNSSNTTHLQEIAISRGIRSFHIDTPERIGDNNSIQHKPLGEDL 358

HSP 2 Score: 44.669 bits (104), Expect = 4.149e-18
Identity = 16/35 (45.71%), Postives = 28/35 (80.00%), Query Frame = -3

Query:  182 EKNWLPKGQITIGVTAGASIPDKAVENVLKKVFEI 286
            E N+LP G + +G+T+GAS PD+ VE+V++K+ ++
Sbjct:  361 ESNFLPAGSVNVGITSGASTPDRVVEHVIQKLIDL 395

The following BLAST results are available for this feature:

BLAST of CV712848 vs. ExPASy Swiss-Prot

BLAST of CV712848 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 33


Match Name	E-value	Identity	Description
ISPH_MICAN	3.666e-20	51.47	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_SYNP2	6.201e-20	53.73	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_PROM4	6.201e-20	48.61	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_GLOVI	8.059e-20	46.97	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_CYAP7	1.774e-19	50.70	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_SYNPW	6.601e-19	50.00	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_CYAP8	8.581e-19	50.70	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_SYNR3	1.451e-18	53.73	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_PROMM	3.191e-18	49.25	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]
ISPH_PROM3	4.149e-18	49.25	4-hydroxy-3-methylbut-2-enyl diphosphate reductase...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS08_0001P17_r Parent Washington Navel Orange Callus cDNA Library UCRCS08-1 Citrus sinensis cDNA clone CS_AEa0001P17, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CV712848 ID=CV712848; Name=CV712848; organism=Citrus sinensis; type=EST; length=834bp
CCCCGGGCTGCAGGTTTACACAAAAACTAAAAATTTCAGATCACATGGGC
ACTAAGAAAAGACTCAGAAGAGGGTTGTGTTTATAATTTTACAATATCTA
ATTGAGAATCACATTGAAATTTATTGATAATATGTACGTTTGGACCTCAG
TTTACGCCAATTGCAAGGCTTCTTCACGCTCGATCTCGAACACCTTTTTA
AGGACATTTTCAACTGCCTTATCTGGGATGGAGGCACCGGCTGTTACACC
GATTGTGATCTGTCCTTTCGGCAGCCAATTTTTTTCTCCACCAGCTCACC
ATGCATTAATTTATGTGCTATTTTGTTTCCGGGACCTATTCTTTTCTCAC
TGTCGATCCAGTATGAGGAGATTCCACGGTCTTCTGCAATCTTTTGAAGG
CTTGAGGTAGTGCTCGAGTTCCACCTGCCAACAAGCAAAATAAGATCGAT
CTTTTCCTCAACCAACTAATACATTGCATCTTGCCGCTCCTGCAAAACTT
GAAACCAAAATCTTTAGCTTAAATTTTACTGAACGCAACTGAATGTTGCA
CAACTAGCTGTTTAACTAGTGGCTGAGTATTGCACTCTTGCGGGCGGAGC
AGTTCGGTCATTGAAAACAAATGGTCAAGTATGTTGTTAGGTTTGGCATA
TCGAGGTTCAAAATTCTGGGTTTTATAAAGATCGTTAAAAGACATGCAGT
GGACAGGAAGTTCTATGACACACAAAATTATAAAAATCTGAAAAGGAAAA
GCCATGAGTTAGAAGAAGAAATTACTTGTGCAGCATAACAAATTGTGTTG
AAGCTTATGAAGTGTTCATTGACATTTTCAACTC