CV712161 | Citrus Genome Database

Overview


Name	CV712161
Unique Name	CV712161
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	825

Libraries


Library Name	Type
Parent Washington Navel Orange Callus cDNA Library UCRCS08-1	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig3195	contig	Csv1_Contig3195:92..915.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A3_CHICK (Eukaryotic initiation factor 4A-III OS=Gallus gallus GN=EIF4A3 PE=2 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 1.375e-62
Identity = 122/188 (64.89%), Postives = 147/188 (78.19%), Query Frame = 1

Query:  205 DFFTSYD-EVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            +F TS + +V  +FD MGL+E+LLRGIYAYGFEKPSAIQQR I    KG DVI Q+QSGTGKTATF   +LQ LD  + + QAL+LAPTRELA QI+K + ALGDY+ V+ HAC+GGT+V ED R L  G HVV GTPGRVFDM+RR+SLR   IKM VLDEADEML++GFK+QIYD+++ LP   QV
Sbjct:   28 EFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQV 215

HSP 2 Score: 26.1794 bits (56), Expect = 1.375e-62
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SAT+P E LE+T KFM
Sbjct:  217 LISATLPHEILEMTNKFM 234

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A3_TAEGU (Eukaryotic initiation factor 4A-III OS=Taeniopygia guttata GN=EIF4A3 PE=2 SV=1)

HSP 1 Score: 234.958 bits (598), Expect = 1.375e-62
Identity = 122/188 (64.89%), Postives = 147/188 (78.19%), Query Frame = 1

Query:  205 DFFTSYD-EVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            +F TS + +V  +FD MGL+E+LLRGIYAYGFEKPSAIQQR I    KG DVI Q+QSGTGKTATF   +LQ LD  + + QAL+LAPTRELA QI+K + ALGDY+ V+ HAC+GGT+V ED R L  G HVV GTPGRVFDM+RR+SLR   IKM VLDEADEML++GFK+QIYD+++ LP   QV
Sbjct:   26 EFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQV 213

HSP 2 Score: 26.1794 bits (56), Expect = 1.375e-62
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SAT+P E LE+T KFM
Sbjct:  215 LISATLPHEILEMTNKFM 232

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A3_MOUSE (Eukaryotic initiation factor 4A-III OS=Mus musculus GN=Eif4a3 PE=2 SV=3)

HSP 1 Score: 234.958 bits (598), Expect = 1.375e-62
Identity = 122/188 (64.89%), Postives = 147/188 (78.19%), Query Frame = 1

Query:  205 DFFTSYD-EVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            +F TS + +V  +FD MGL+E+LLRGIYAYGFEKPSAIQQR I    KG DVI Q+QSGTGKTATF   +LQ LD  + + QAL+LAPTRELA QI+K + ALGDY+ V+ HAC+GGT+V ED R L  G HVV GTPGRVFDM+RR+SLR   IKM VLDEADEML++GFK+QIYD+++ LP   QV
Sbjct:   27 EFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSVSVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQV 214

HSP 2 Score: 26.1794 bits (56), Expect = 1.375e-62
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SAT+P E LE+T KFM
Sbjct:  216 LISATLPHEILEMTNKFM 233

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A3_HUMAN (Eukaryotic initiation factor 4A-III OS=Homo sapiens GN=EIF4A3 PE=1 SV=4)

HSP 1 Score: 234.958 bits (598), Expect = 1.375e-62
Identity = 122/188 (64.89%), Postives = 147/188 (78.19%), Query Frame = 1

Query:  205 DFFTSYD-EVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            +F TS + +V  +FD MGL+E+LLRGIYAYGFEKPSAIQQR I    KG DVI Q+QSGTGKTATF   +LQ LD  + + QAL+LAPTRELA QI+K + ALGDY+ V+ HAC+GGT+V ED R L  G HVV GTPGRVFDM+RR+SLR   IKM VLDEADEML++GFK+QIYD+++ LP   QV
Sbjct:   27 EFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPTRELAVQIQKGLLALGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQV 214

HSP 2 Score: 26.1794 bits (56), Expect = 1.375e-62
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SAT+P E LE+T KFM
Sbjct:  216 LISATLPHEILEMTNKFM 233

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: FAL1_USTMA (ATP-dependent RNA helicase FAL1 OS=Ustilago maydis GN=FAL1 PE=3 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 8.812e-62
Identity = 117/180 (65.00%), Postives = 142/180 (78.89%), Query Frame = 1

Query:  226 EVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            +V  +FDAMGL+E+LLRGIYAY FEKPSAIQQR I+P  +G DVI QAQSGTGKTATF   +LQ +D  L + QALVL+PTRELA QI+ V+ ALGDYL V+ HAC+GGTSV ED R L  G H+V GTPGRV+DM+RR+ LR   IKM +LDE+DE+L+ GFKDQIYD+++ LP   QV
Sbjct:   21 KVATTFDAMGLKEDLLRGIYAYNFEKPSAIQQRAILPIIRGRDVIAQAQSGTGKTATFSISMLQSIDTTLRETQALVLSPTRELAIQIQSVVLALGDYLNVQCHACIGGTSVGEDIRKLDYGQHIVSGTPGRVYDMIRRRHLRTKNIKMLILDESDELLNMGFKDQIYDVYRYLPPSTQV 200

HSP 2 Score: 25.409 bits (54), Expect = 8.812e-62
Identity = 10/18 (55.56%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SAT+P + LE+T KFM
Sbjct:  202 LLSATLPQDVLEMTSKFM 219

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A3_MACFA (Eukaryotic initiation factor 4A-III OS=Macaca fascicularis GN=EIF4A3 PE=2 SV=3)

HSP 1 Score: 231.491 bits (589), Expect = 1.498e-61
Identity = 120/188 (63.83%), Postives = 145/188 (77.13%), Query Frame = 1

Query:  205 DFFTSYD-EVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            +F TS + +V  +FD MGL+E+LLRGIYAYGFEKPSAIQQR I    KG DVI Q+QSGTGKTATF   +LQ LD  + + QAL+LAP RELA QI+K +  LGDY+ V+ HAC+GGT+V ED R L  G HVV GTPGRVFDM+RR+SLR   IKM VLDEADEML++GFK+QIYD+++ LP   QV
Sbjct:   27 EFETSEEVDVTPTFDTMGLREDLLRGIYAYGFEKPSAIQQRAIKQIIKGRDVIAQSQSGTGKTATFSISVLQCLDIQVRETQALILAPARELAVQIQKGLLTLGDYMNVQCHACIGGTNVGEDIRKLDYGQHVVAGTPGRVFDMIRRRSLRTRAIKMLVLDEADEMLNKGFKEQIYDVYRYLPPATQV 214

HSP 2 Score: 26.1794 bits (56), Expect = 1.498e-61
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SAT+P E LE+T KFM
Sbjct:  216 LISATLPHEILEMTNKFM 233

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: FAL1_CRYNE (ATP-dependent RNA helicase FAL1 OS=Cryptococcus neoformans GN=FAL1 PE=3 SV=1)

HSP 1 Score: 231.106 bits (588), Expect = 2.547e-61
Identity = 116/179 (64.80%), Postives = 142/179 (79.33%), Query Frame = 1

Query:  229 VYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            V  +F+A+ L+E+LLRGIYAY FEKPSAIQQR I+P  +G DVI QAQSGTGKTATF   +LQ +D  L + QALVL+PTRELA QI+ V+ ALGDY+ V  HAC+GGTSV ED R L+AG  VV GTPGRVFDM+RR++LR   IKM +LDE+DE+L++GFKDQIYDI++ LP   QV
Sbjct:   21 VAPTFEALNLKEDLLRGIYAYNFEKPSAIQQRAIIPIIRGRDVIAQAQSGTGKTATFSISMLQSIDTNLRETQALVLSPTRELAVQIQTVVLALGDYMNVSCHACIGGTSVGEDIRKLEAGQQVVSGTPGRVFDMIRRRNLRTKDIKMLILDESDELLNKGFKDQIYDIYRYLPPATQV 199

HSP 2 Score: 25.7942 bits (55), Expect = 2.547e-61
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            V SAT+P + LE+T KFM
Sbjct:  201 VVSATLPHDVLEMTTKFM 218

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: RH34_ORYSJ (DEAD-box ATP-dependent RNA helicase 34 OS=Oryza sativa subsp. japonica GN=Os03g0566800 PE=2 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 5.645e-61
Identity = 111/180 (61.67%), Postives = 142/180 (78.89%), Query Frame = 1

Query:  226 EVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            EV  SFD MG++E+LLRGIYAYGFEKPSAIQQR ++P   G DVI QAQSGTGKT+     + Q +D  + + QAL+L+PTRELA Q E+VM A+GDY+ ++VHAC+GG S+ ED R L+ GVHVV GTPGRV DM++R++LR   IK+ +LDEADEML RGFKDQIYD+++ LP ++QV
Sbjct:   28 EVISSFDQMGIREDLLRGIYAYGFEKPSAIQQRAVLPIISGRDVIAQAQSGTGKTSMISLSVCQIVDTAVREVQALILSPTRELAAQTERVMLAIGDYINIQVHACIGGKSIGEDIRKLEHGVHVVSGTPGRVCDMIKRRTLRTRAIKLLILDEADEMLGRGFKDQIYDVYRYLPPELQV 207

HSP 2 Score: 25.7942 bits (55), Expect = 5.645e-61
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SAT+P E LE+T KFM
Sbjct:  209 LISATLPHEILEMTSKFM 226

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: IF4A_ASHGO (ATP-dependent RNA helicase eIF4A OS=Ashbya gossypii GN=TIF1 PE=3 SV=1)

HSP 1 Score: 227.639 bits (579), Expect = 1.252e-60
Identity = 112/196 (57.14%), Postives = 144/196 (73.47%), Query Frame = 1

Query:  178 NELLSTDGQDFFTSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            + + + +  +  T+YD++   FD + L+E LLRGIY YGF  PSAIQQR I+P  +G DV+ QAQSGTGKT TF    LQ++D  +   QAL+LAPTRELA QI+KV+ AL  ++ VKVHAC+GGT  RED   L+AG  +VVGTPGRVFDM+ R++ + D+IKMF+LDEADEMLS GFK+QIY IF +LP   QV
Sbjct:    3 DSITNPENSEIQTNYDKIVHKFDELKLKEVLLRGIYGYGFVDPSAIQQRAILPIIEGHDVLAQAQSGTGKTGTFSIAALQRIDESIKAPQALILAPTRELALQIQKVVMALALHMDVKVHACIGGTDPREDAEALRAGAQIVVGTPGRVFDMIERRNFKTDHIKMFILDEADEMLSSGFKEQIYKIFTMLPPTTQV 198

HSP 2 Score: 26.9498 bits (58), Expect = 1.252e-60
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SATMP E L++T KFM
Sbjct:  200 LLSATMPKEVLDVTDKFM 217

[Close]

BLAST of CV712161 vs. ExPASy Swiss-Prot
Match: RH2_ORYSJ (DEAD-box ATP-dependent RNA helicase 2 OS=Oryza sativa subsp. japonica GN=Os01g0639100 PE=2 SV=1)

HSP 1 Score: 227.254 bits (578), Expect = 3.616e-60
Identity = 109/180 (60.56%), Postives = 142/180 (78.89%), Query Frame = 1

Query:  226 EVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADEMLSRGFKDQIYDIFQLLPAKVQV 765
            EV  SFD MG++++LLRGIYAYGFEKPSAIQQR ++P   G DVI QAQSGTGKT+     + Q +D  + + QAL+L+PTRELA Q E+VM A+GD++ ++VHAC+GG S+ ED R L+ GVHVV GTPGRV DM++R++LR   IK+ +LDEADEML RGFKDQIYD+++ LP ++QV
Sbjct:   28 EVISSFDQMGIRDDLLRGIYAYGFEKPSAIQQRAVLPIISGRDVIAQAQSGTGKTSMISLSVCQIVDTAVREVQALILSPTRELAAQTERVMLAIGDFINIQVHACIGGKSIGEDIRKLEHGVHVVSGTPGRVCDMIKRRTLRTRAIKLLILDEADEMLGRGFKDQIYDVYRYLPPELQV 207

HSP 2 Score: 25.7942 bits (55), Expect = 3.616e-60
Identity = 11/18 (61.11%), Postives = 14/18 (77.78%), Query Frame = 2

Query:  770 VFSATMPPEALEITRKFM 823
            + SAT+P E LE+T KFM
Sbjct:  209 LISATLPHEILEMTSKFM 226

The following BLAST results are available for this feature:

BLAST of CV712161 vs. ExPASy Swiss-Prot

BLAST of CV712161 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
IF4A3_CHICK	1.375e-62	64.89	Eukaryotic initiation factor 4A-III OS=Gallus gall...	[more]
IF4A3_TAEGU	1.375e-62	64.89	Eukaryotic initiation factor 4A-III OS=Taeniopygia...	[more]
IF4A3_MOUSE	1.375e-62	64.89	Eukaryotic initiation factor 4A-III OS=Mus musculu...	[more]
IF4A3_HUMAN	1.375e-62	64.89	Eukaryotic initiation factor 4A-III OS=Homo sapien...	[more]
FAL1_USTMA	8.812e-62	65.00	ATP-dependent RNA helicase FAL1 OS=Ustilago maydis...	[more]
IF4A3_MACFA	1.498e-61	63.83	Eukaryotic initiation factor 4A-III OS=Macaca fasc...	[more]
FAL1_CRYNE	2.547e-61	64.80	ATP-dependent RNA helicase FAL1 OS=Cryptococcus ne...	[more]
RH34_ORYSJ	5.645e-61	61.67	DEAD-box ATP-dependent RNA helicase 34 OS=Oryza sa...	[more]
IF4A_ASHGO	1.252e-60	57.14	ATP-dependent RNA helicase eIF4A OS=Ashbya gossypi...	[more]
RH2_ORYSJ	3.616e-60	60.56	DEAD-box ATP-dependent RNA helicase 2 OS=Oryza sat...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS08_0001A04_f Parent Washington Navel Orange Callus cDNA Library UCRCS08-1 Citrus sinensis cDNA clone CS_AEa0001A04, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CV712161 ID=CV712161; Name=CV712161; organism=Citrus sinensis; type=EST; length=825bp
AGTTTGGTAACCCTAGCAACTATTTTCTCTTAAATCAATCTTCTCCTTCT
CTTTGAAGAAAAAAGAAAACCAACTCTTTCTCTCGATTTTAATCACCATC
ATCAAAGCTTTGCAGATATGGCTGGTTTGGCACCCGAAGGATCTCAATTT
GATGCTCGTCAGTATGACACCAAGATGAATGAACTGCTCTCTACTGATGG
GCAAGATTTCTTCACTTCTTATGATGAAGTTTATGACAGTTTTGATGCAA
TGGGACTGCAGGAAAACCTTTTGAGGGGAATCTATGCCTATGGGTTTGAG
AAGCCCTCTGCTATTCAGCAAAGGGGAATTGTTCCCTTCTGCAAGGGACT
TGACGTCATTCAGCAGGCTCAGTCTGGAACTGGGAAAACAGCAACTTTCT
GCTCTGGAATTTTGCAGCAACTTGATTATGGTCTTGTCCAATGTCAGGCC
CTGGTATTGGCACCCACTAGGGAACTAGCTCAACAGATTGAGAAAGTAAT
GAGGGCACTTGGTGATTATCTTGGTGTTAAGGTTCATGCTTGTGTCGGTG
GGACAAGTGTTCGCGAGGATCAGCGCATTCTTCAAGCTGGTGTTCATGTT
GTTGTTGGCACTCCTGGTCGTGTGTTTGACATGCTGCGCCGACAGTCTTT
ACGCCCTGATTACATTAAGATGTTTGTACTGGATGAGGCTGATGAAATGC
TTTCCCGTGGTTTTAAGGATCAGATCTATGATATTTTCCAGCTGCTTCCA
GCTAAAGTTCAGGTGGGGGGTTTTCTCTGCTACTATGCCTCCTGAGGCCT
TGGAGATTACAAGGAAGTTTATGAA