EY715966 | Citrus Genome Database

Overview


Name	EY715966
Unique Name	EY715966
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	948

Libraries


Library Name	Type
Sweet orange fruit, development stadium (3 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
EY715966_ssr767	microsatellite	EY715966_ssr767:767..778.	BLAST: Citrus ESTs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBE2B_MOUSE (Ubiquitin-conjugating enzyme E2 B OS=Mus musculus GN=Ube2b PE=1 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 4.002e-38
Identity = 64/89 (71.91%), Postives = 79/89 (88.76%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICL 321
            MSTPA++RLMRDFKRL+ DPP G+SGAP +NNIM WNAVIFGP+ TP++ GTFKL ++F+E+YPNKPPT RF+ +MFHP +YADGSICL
Sbjct:    1 MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICL 89

HSP 2 Score: 30.4166 bits (67), Expect = 4.002e-38
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = 2

Query:  323 DILQNQWSPIFDVVAIL 373
            DILQN+WSP +DV +IL
Sbjct:   90 DILQNRWSPTYDVSSIL 106

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBE2B_HUMAN (Ubiquitin-conjugating enzyme E2 B OS=Homo sapiens GN=UBE2B PE=1 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 4.002e-38
Identity = 64/89 (71.91%), Postives = 79/89 (88.76%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICL 321
            MSTPA++RLMRDFKRL+ DPP G+SGAP +NNIM WNAVIFGP+ TP++ GTFKL ++F+E+YPNKPPT RF+ +MFHP +YADGSICL
Sbjct:    1 MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICL 89

HSP 2 Score: 30.4166 bits (67), Expect = 4.002e-38
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = 2

Query:  323 DILQNQWSPIFDVVAIL 373
            DILQN+WSP +DV +IL
Sbjct:   90 DILQNRWSPTYDVSSIL 106

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBE2B_BOVIN (Ubiquitin-conjugating enzyme E2 B OS=Bos taurus GN=UBE2B PE=2 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 4.002e-38
Identity = 64/89 (71.91%), Postives = 79/89 (88.76%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICL 321
            MSTPA++RLMRDFKRL+ DPP G+SGAP +NNIM WNAVIFGP+ TP++ GTFKL ++F+E+YPNKPPT RF+ +MFHP +YADGSICL
Sbjct:    1 MSTPARRRLMRDFKRLQEDPPVGVSGAPSENNIMQWNAVIFGPEGTPFEDGTFKLVIEFSEEYPNKPPTVRFLSKMFHPNVYADGSICL 89

HSP 2 Score: 30.4166 bits (67), Expect = 4.002e-38
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = 2

Query:  323 DILQNQWSPIFDVVAIL 373
            DILQN+WSP +DV +IL
Sbjct:   90 DILQNRWSPTYDVSSIL 106

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBC1_CAEEL (Ubiquitin-conjugating enzyme E2 1 OS=Caenorhabditis elegans GN=ubc-1 PE=1 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 3.296e-37
Identity = 59/89 (66.29%), Postives = 80/89 (89.89%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICL 321
            M+TP+++RLMRDFK+L+ DPPAG+SGAP ++NI+ W A+IFGP +TP++ GTFKL+L+FTE+YPNKPPT +F+ +MFHP +YADGSICL
Sbjct:    1 MTTPSRRRLMRDFKKLQEDPPAGVSGAPTEDNILTWEAIIFGPQETPFEDGTFKLSLEFTEEYPNKPPTVKFISKMFHPNVYADGSICL 89

HSP 2 Score: 32.3426 bits (72), Expect = 3.296e-37
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = 2

Query:  323 DILQNQWSPIFDVVAIL 373
            DILQN+WSP +DV AIL
Sbjct:   90 DILQNRWSPTYDVAAIL 106

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBC2_USTMA (Ubiquitin-conjugating enzyme E2 2 OS=Ustilago maydis GN=UBC2 PE=3 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 2.269e-35
Identity = 61/89 (68.54%), Postives = 74/89 (83.15%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICL 321
            MSTP+KKRL+RDFKRL  DPP GISGAP  +N+M+WNAVIFGP DTP++ GTFKL L F E YPNKPPT +F+ +MFHP +YA+G +CL
Sbjct:    1 MSTPSKKRLIRDFKRLSTDPPGGISGAPCADNLMIWNAVIFGPADTPFEDGTFKLVLTFDESYPNKPPTVKFLSKMFHPNVYANGELCL 89

HSP 2 Score: 32.3426 bits (72), Expect = 2.269e-35
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = 2

Query:  323 DILQNQWSPIFDVVAIL 373
            DILQN+WSP +DV AIL
Sbjct:   90 DILQNRWSPTYDVAAIL 106

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBC2_DICDI (Ubiquitin-conjugating enzyme E2 2 OS=Dictyostelium discoideum GN=ubc2 PE=3 SV=2)

HSP 1 Score: 144.821 bits (364), Expect = 6.788e-34
Identity = 72/148 (48.65%), Postives = 96/148 (64.86%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICLGYIAKSVESYI*CCSYTGPVFGRWCGIRVRFFLLTPKTTRVLLDAKRNNRPKSREILEQ 498
            MST A++RLMRDFKRL+ DPPAGISGAP +NNI++W AVIFGPDD+ W+G TFKL+LQF+E+YPN  PT +F+ +MFHP +YADGSICL  +           +    +    C           ++ R+  + KR    K +EI+EQ
Sbjct:    1 MSTAARRRLMRDFKRLQSDPPAGISGAPLENNILMWQAVIFGPDDSIWEGATFKLSLQFSEEYPNDAPTVKFLSKMFHPNVYADGSICLDILQNQWSPIYDIAAILTSIQSLLCDPNPN-SPANSESARLFRENKREYNRKVKEIVEQ 147

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBC2_CRYNE (Ubiquitin-conjugating enzyme E2 2 OS=Cryptococcus neoformans GN=UBC2 PE=3 SV=1)

HSP 1 Score: 133.65 bits (335), Expect = 7.069e-34
Identity = 59/89 (66.29%), Postives = 74/89 (83.15%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICL 321
            MST AK+RL+RDFKRL  D P GISG+P  +NIM+WNAVIFGP +TP++ G+F+LTL FT+ YPNKPPT RF+ +MFHP IYA+G +CL
Sbjct:    1 MSTAAKRRLIRDFKRLTSDAPIGISGSPNPDNIMVWNAVIFGPPETPFEDGSFRLTLTFTDAYPNKPPTVRFISKMFHPNIYANGELCL 89

HSP 2 Score: 32.3426 bits (72), Expect = 7.069e-34
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = 2

Query:  323 DILQNQWSPIFDVVAIL 373
            DILQN+WSP +DV AIL
Sbjct:   90 DILQNRWSPTYDVAAIL 106

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBC2_SCHPO (Ubiquitin-conjugating enzyme E2 2 OS=Schizosaccharomyces pombe GN=rhp6 PE=1 SV=3)

HSP 1 Score: 132.494 bits (332), Expect = 1.185e-33
Identity = 57/89 (64.04%), Postives = 72/89 (80.90%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICL 321
            MST A++RLMRDFKR+++DPPAG+S +P  +N+MLWNAVI GP DTP++ GTFKL L F E YPNKPP  +FV  MFHP +YA+G +CL
Sbjct:    1 MSTTARRRLMRDFKRMQQDPPAGVSASPVSDNVMLWNAVIIGPADTPFEDGTFKLVLSFDEQYPNKPPLVKFVSTMFHPNVYANGELCL 89

HSP 2 Score: 32.3426 bits (72), Expect = 1.185e-33
Identity = 13/17 (76.47%), Postives = 15/17 (88.24%), Query Frame = 2

Query:  323 DILQNQWSPIFDVVAIL 373
            DILQN+WSP +DV AIL
Sbjct:   90 DILQNRWSPTYDVAAIL 106

HSP 3 Score: 20.7866 bits (42), Expect = 1.185e-33
Identity = 9/19 (47.37%), Postives = 13/19 (68.42%), Query Frame = 3

Query:  375 TCIRSLVWDPGEIFPAHSE 431
            T I+SL+ DP    PA++E
Sbjct:  107 TSIQSLLNDPNNASPANAE 125

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBC2_DEBHA (Ubiquitin-conjugating enzyme E2 2 OS=Debaryomyces hansenii GN=UBC2 PE=3 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 2.194e-32
Identity = 55/89 (61.80%), Postives = 72/89 (80.90%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICL 321
            MSTPAK+RLMRDFKR+++D P+G+S +P  +N+M WNAVI GP DTP++ GTF+L LQF E YPNKPP+ +F+  MFHP +YA G +CL
Sbjct:    1 MSTPAKRRLMRDFKRMQQDAPSGVSASPLPDNVMSWNAVIIGPADTPFEDGTFRLILQFDEQYPNKPPSVKFISEMFHPNVYASGELCL 89

HSP 2 Score: 30.4166 bits (67), Expect = 2.194e-32
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = 2

Query:  323 DILQNQWSPIFDVVAIL 373
            DILQN+WSP +DV +IL
Sbjct:   90 DILQNRWSPTYDVSSIL 106

[Close]

BLAST of EY715966 vs. ExPASy Swiss-Prot
Match: UBC2_NEUCR (Ubiquitin-conjugating enzyme E2 2 OS=Neurospora crassa GN=mus-8 PE=3 SV=3)

HSP 1 Score: 127.487 bits (319), Expect = 6.341e-32
Identity = 53/89 (59.55%), Postives = 70/89 (78.65%), Query Frame = 1

Query:   55 MSTPAKKRLMRDFKRLRRDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLTLQFTEDYPNKPPTGRFVIRMFHPYIYADGSICL 321
            MST A++RLMRDFKR++ DPPAG+S +P  +N+M WNAVI GP DTP++ GTF+L + F E YPNKPP+ +F+  MFHP +YA G +CL
Sbjct:    1 MSTAARRRLMRDFKRMQTDPPAGVSASPVPDNVMTWNAVIIGPADTPFEDGTFRLVMHFEEQYPNKPPSVKFISEMFHPNVYATGELCL 89

HSP 2 Score: 31.9574 bits (71), Expect = 6.341e-32
Identity = 12/17 (70.59%), Postives = 15/17 (88.24%), Query Frame = 2

Query:  323 DILQNQWSPIFDVVAIL 373
            DILQN+WSP +DV A+L
Sbjct:   90 DILQNRWSPTYDVAAVL 106

The following BLAST results are available for this feature:

BLAST of EY715966 vs. ExPASy Swiss-Prot

BLAST of EY715966 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 147


Match Name	E-value	Identity	Description
UBE2B_MOUSE	4.002e-38	71.91	Ubiquitin-conjugating enzyme E2 B OS=Mus musculus ...	[more]
UBE2B_HUMAN	4.002e-38	71.91	Ubiquitin-conjugating enzyme E2 B OS=Homo sapiens ...	[more]
UBE2B_BOVIN	4.002e-38	71.91	Ubiquitin-conjugating enzyme E2 B OS=Bos taurus GN...	[more]
UBC1_CAEEL	3.296e-37	66.29	Ubiquitin-conjugating enzyme E2 1 OS=Caenorhabditi...	[more]
UBC2_USTMA	2.269e-35	68.54	Ubiquitin-conjugating enzyme E2 2 OS=Ustilago mayd...	[more]
UBC2_DICDI	6.788e-34	48.65	Ubiquitin-conjugating enzyme E2 2 OS=Dictyostelium...	[more]
UBC2_CRYNE	7.069e-34	66.29	Ubiquitin-conjugating enzyme E2 2 OS=Cryptococcus ...	[more]
UBC2_SCHPO	1.185e-33	64.04	Ubiquitin-conjugating enzyme E2 2 OS=Schizosacchar...	[more]
UBC2_DEBHA	2.194e-32	61.80	Ubiquitin-conjugating enzyme E2 2 OS=Debaryomyces ...	[more]
UBC2_NEUCR	6.341e-32	59.55	Ubiquitin-conjugating enzyme E2 2 OS=Neurospora cr...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-702-060-H01-CT.F Sweet orange fruit, development stadium (3 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY715966 ID=EY715966; Name=EY715966; organism=Citrus sinensis; type=EST; length=948bp
CTTTTCTCTAGAACTTTCCTCTCTCGTTCTCTCTCTGTAGAACACGACGT
CGCCATGTCAACACCGGCGAAGAAGCGGCTGATGCGGGACTTCAAGCGCC
TCCGGCGAGATCCACCCGCCGGCATCAGCGGTGCCCCTCAAGACAACAAC
ATCATGTTGTGGAACGCCGTTATTTTTGGGCCCGATGATACTCCCTGGGA
CGGAGGTACGTTCAAGTTGACTCTACAATTCACAGAGGATTATCCAAATA
AACCCCCAACAGGTCGTTTCGTCATCCGGATGTTTCATCCCTATATCTAT
GCAGATGGAAGTATTTGTTTGGGATATATTGCAAAATCAGTGGAGTCCTA
TATTTGATGTTGTAGCTATACTGGACCTGTATTCGGTCGCTGGTGTGGGA
TCCGGGTGAGATTTTTCCTGCTCACTCCGAAGACGACTCGGGTGTTGCTT
GATGCTAAGCGAAATAATAGACCAAAGAGTCGGGAAATTCTGGAACAAAA
ATAGATAAGCGGAGTATAAGGCAGAGACAGAAGGGGGGGTGCGCCGCGCA
GAGTAATGAGGAAATGGTCGGGGGGAGACTTTGTGTTACTTCAACGGGCG
GCCCTCACAACGGCCCGAGAGAGGGGGGGTGGGGAATTATGTTCGGTAGG
TGTGGGGGGTTCGGAGGGGGAGGGGCGCGGGGGGTCGCGAGACGCGGGAG
AAAACGAGAACAGGGGGGGAGGGGAGGAAGACGGGATAGAACATAGCGGG
GGGGGAAGTATAGGCCGGGAGGGAGGGAAGGATAAAGGGGAGGTGGGAGG
GGGAAGGGAGGATAGAAGAGGGGGAAGCGGAGAAGGAGAAGAAGGAGGAG
GGGAGGGGAGANAGGGGGGAGGGGATGAGTGGGACGGAGGGATGGAGGAG
GGAGGAGAGAGGGAGAGAGAGGGTGATGGAAGGAAAGCGAGGTGCCTG