DY257175

Overview
NameDY257175
Unique NameDY257175
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length969
Libraries
Library NameType
AbsCFruitcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DY257175 vs. ExPASy Swiss-Prot
Match: IF4A_CHAGB (ATP-dependent RNA helicase eIF4A OS=Chaetomium globosum GN=TIF1 PE=3 SV=1)

HSP 1 Score: 342.428 bits (877), Expect = 2.285e-93
Identity = 162/219 (73.97%), Postives = 197/219 (89.95%), Query Frame = 2 
Query:    2 LSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMRGYNFTVSSMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKNDDIKILRDIEQYYSTQIDEMPMNVADLI 658
            LSRGF +QIYD+++ LP   QVVL+SAT+P ++LE+TTKFM DPV+ILVK+DELTLEGIKQF++AVE+E+WK DTL DLY+T+TITQAVIFCNT+RKVDWLT+K++  +FTVS+MHGDM Q +RD IM EFRSG++RVLI TD+ ARG+DVQQVSLVINYDLP NRE YIHRIGR GRFGRKGVAINFV  DD++++R+IEQ+YSTQI+EMPMNVADLI
Sbjct:  179 LSRGFTEQIYDIFQLLPQSTQVVLLSATMPQDVLEVTTKFMRDPVRILVKKDELTLEGIKQFYIAVEKEDWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLQARDFTVSAMHGDMDQTQRDLIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 397
[Close]
BLAST of DY257175 vs. ExPASy Swiss-Prot
Match: IF4A_PHANO (ATP-dependent RNA helicase eIF4A OS=Phaeosphaeria nodorum GN=TIF1 PE=3 SV=1)

HSP 1 Score: 342.043 bits (876), Expect = 2.984e-93
Identity = 162/219 (73.97%), Postives = 196/219 (89.50%), Query Frame = 2 
Query:    2 LSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMRGYNFTVSSMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKNDDIKILRDIEQYYSTQIDEMPMNVADLI 658
            LSRGF +QIYD+++ LP   QVVL+SAT+P ++LE+TTKFM DPV+ILVK+DELTLEGIKQF++AVE+E+WK DTL DLY+T+TITQAVIFCNT+RKVDWLT+K+   +FTVS+MHGDM Q +RD IM EFRSG++RVLI TD+ ARG+DVQQVSLVINYDLP NRE YIHRIGR GRFGRKGVAINFV  DD++++R+IEQ+YSTQI+EMPMNVADLI
Sbjct:  178 LSRGFTEQIYDIFQLLPQSTQVVLLSATMPQDVLEVTTKFMRDPVRILVKKDELTLEGIKQFYIAVEKEDWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMDQAQRDVIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 396
[Close]
BLAST of DY257175 vs. ExPASy Swiss-Prot
Match: IF4A_NEUCR (ATP-dependent RNA helicase eIF4A OS=Neurospora crassa GN=tif-1 PE=3 SV=2)

HSP 1 Score: 341.273 bits (874), Expect = 5.090e-93
Identity = 162/219 (73.97%), Postives = 196/219 (89.50%), Query Frame = 2 
Query:    2 LSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMRGYNFTVSSMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKNDDIKILRDIEQYYSTQIDEMPMNVADLI 658
            LSRGF +QIYD+++ LP   QVVL+SAT+P ++LE+TTKFM +PV+ILVK+DELTLEGIKQF++AVE+EEWK DTL DLY+T+TITQAVIFCNT+RKVDWLT+K+   +FTVS+MHGDM Q +RD IM EFRSG++RVLI TD+ ARG+DVQQVSLVINYDLP NRE YIHRIGR GRFGRKGVAINFV  DD++++R+IEQ+YSTQI+EMPMNVADLI
Sbjct:  179 LSRGFTEQIYDIFQLLPQSTQVVLLSATMPQDVLEVTTKFMREPVRILVKKDELTLEGIKQFYIAVEKEEWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMDQAQRDLIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 397
[Close]
BLAST of DY257175 vs. ExPASy Swiss-Prot
Match: IF4A_ASPOR (ATP-dependent RNA helicase eIF4A OS=Aspergillus oryzae GN=tif1 PE=3 SV=1)

HSP 1 Score: 341.273 bits (874), Expect = 5.090e-93
Identity = 162/219 (73.97%), Postives = 195/219 (89.04%), Query Frame = 2 
Query:    2 LSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMRGYNFTVSSMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKNDDIKILRDIEQYYSTQIDEMPMNVADLI 658
            LSRGF +QIYD+++ LP   QVVL+SAT+P ++LE+TTKFM DPV+ILVK+ ELTLEGIKQF++AVE+EEWK DTL DLY+T+TITQAVIFCNT+RKVDWLT+K+   +FTVS+MHGDM Q +RD IM EFRSG++RVLI TD+ ARG+DVQQVSLVINYDLP NRE YIHRIGR GRFGRKGVAINFV  DD++++R+IEQ+YSTQI+EMPMNVADLI
Sbjct:  203 LSRGFTEQIYDIFQLLPQSTQVVLLSATMPQDVLEVTTKFMRDPVRILVKKQELTLEGIKQFYIAVEKEEWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMEQSQRDVIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 421
[Close]
BLAST of DY257175 vs. ExPASy Swiss-Prot
Match: IF4A_ASPCL (ATP-dependent RNA helicase eIF4A OS=Aspergillus clavatus GN=tif1 PE=3 SV=1)

HSP 1 Score: 341.273 bits (874), Expect = 5.090e-93
Identity = 162/219 (73.97%), Postives = 195/219 (89.04%), Query Frame = 2 
Query:    2 LSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMRGYNFTVSSMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKNDDIKILRDIEQYYSTQIDEMPMNVADLI 658
            LSRGF +QIYD+++ LP   QVVL+SAT+P ++LE+TTKFM DPV+ILVK+ ELTLEGIKQF++AVE+EEWK DTL DLY+T+TITQAVIFCNT+RKVDWLT+K+   +FTVS+MHGDM Q +RD IM EFRSG++RVLI TD+ ARG+DVQQVSLVINYDLP NRE YIHRIGR GRFGRKGVAINFV  DD++++R+IEQ+YSTQI+EMPMNVADLI
Sbjct:  180 LSRGFTEQIYDIFQLLPQSTQVVLLSATMPQDVLEVTTKFMRDPVRILVKKQELTLEGIKQFYIAVEKEEWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMEQSQRDVIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 398
[Close]
BLAST of DY257175 vs. ExPASy Swiss-Prot
Match: IF4A_NEOFI (ATP-dependent RNA helicase eIF4A OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) GN=tif1 PE=3 SV=1)

HSP 1 Score: 340.887 bits (873), Expect = 6.648e-93
Identity = 162/219 (73.97%), Postives = 195/219 (89.04%), Query Frame = 2 
Query:    2 LSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMRGYNFTVSSMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKNDDIKILRDIEQYYSTQIDEMPMNVADLI 658
            LSRGF +QIYD+++ LP   QVVL+SAT+P ++LE+TTKFM DPV+ILVK+ ELTLEGIKQF++AVE+EEWK DTL DLY+T+TITQAVIFCNT+RKVDWLT+K+   +FTVS+MHGDM Q +RD IM EFRSG++RVLI TD+ ARG+DVQQVSLVINYDLP NRE YIHRIGR GRFGRKGVAINFV  DD++++R+IEQ+YSTQI+EMPMNVADLI
Sbjct:  180 LSRGFTEQIYDIFQLLPQSTQVVLLSATMPQDVLEVTTKFMRDPVRILVKKQELTLEGIKQFYIAVEKEEWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMEQAQRDVIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 398
[Close]
BLAST of DY257175 vs. ExPASy Swiss-Prot
Match: IF4A_EMENI (ATP-dependent RNA helicase eIF4A OS=Emericella nidulans GN=tif1 PE=3 SV=1)

HSP 1 Score: 340.887 bits (873), Expect = 6.648e-93
Identity = 162/219 (73.97%), Postives = 195/219 (89.04%), Query Frame = 2 
Query:    2 LSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMRGYNFTVSSMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKNDDIKILRDIEQYYSTQIDEMPMNVADLI 658
            LSRGF +QIYD+++ LP   QVVL+SAT+P ++LE+TTKFM DPV+ILVK+ ELTLEGIKQF++AVE+EEWK DTL DLY+T+TITQAVIFCNT+RKVDWLT+K+   +FTVS+MHGDM Q +RD IM EFRSG++RVLI TD+ ARG+DVQQVSLVINYDLP NRE YIHRIGR GRFGRKGVAINFV  DD++++R+IEQ+YSTQI+EMPMNVADLI
Sbjct:  203 LSRGFTEQIYDIFQLLPQSTQVVLLSATMPQDVLEVTTKFMRDPVRILVKKQELTLEGIKQFYIAVEKEEWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMEQAQRDVIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 421
[Close]
BLAST of DY257175 vs. ExPASy Swiss-Prot
Match: IF4A_ASPTN (ATP-dependent RNA helicase eIF4A OS=Aspergillus terreus (strain NIH 2624 / FGSC A1156) GN=tif1 PE=3 SV=2)

HSP 1 Score: 340.117 bits (871), Expect = 1.134e-92
Identity = 162/219 (73.97%), Postives = 195/219 (89.04%), Query Frame = 2 
Query:    2 LSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMRGYNFTVSSMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKNDDIKILRDIEQYYSTQIDEMPMNVADLI 658
            LSRGF +QIYD+++ LP   QVVL+SAT+P ++LE+TTKFM DPV+ILVK+ ELTLEGIKQF++AVE+EEWK DTL DLY+T+TITQAVIFCNT+RKVDWLT+K+   +FTVS+MHGDM Q +RD IM EFRSG++RVLI TD+ ARG+DVQQVSLVINYDLP NRE YIHRIGR GRFGRKGVAINFV  DD++++R+IEQ+YSTQI+EMPMNVADLI
Sbjct:  178 LSRGFTEQIYDIFQLLPQATQVVLLSATMPQDVLEVTTKFMRDPVRILVKKQELTLEGIKQFYIAVEKEEWKLDTLSDLYETVTITQAVIFCNTRRKVDWLTDKLTARDFTVSAMHGDMEQSQRDIIMKEFRSGSSRVLIATDLLARGIDVQQVSLVINYDLPANRENYIHRIGRGGRFGRKGVAINFVTADDVRMMREIEQFYSTQIEEMPMNVADLI 396
[Close]
BLAST of DY257175 vs. ExPASy Swiss-Prot
Match: IF4A2_RAT (Eukaryotic initiation factor 4A-II OS=Rattus norvegicus GN=Eif4a2 PE=1 SV=1)

HSP 1 Score: 339.732 bits (870), Expect = 1.481e-92
Identity = 163/219 (74.43%), Postives = 194/219 (88.58%), Query Frame = 2 
Query:    2 LSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMRGYNFTVSSMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKNDDIKILRDIEQYYSTQIDEMPMNVADLI 658
            LSRGFKDQIY++++ L   +QVVL+SAT+P ++LE+T KFM DP++ILVK++ELTLEGIKQF++ VEREEWK DTLCDLY+TLTITQAVIF NT+RKVDWLTEKM   +FTVS++HGDM QKERD IM EFRSG++RVLITTD+ ARG+DVQQVSLVINYDLP NRE YIHRIGR GRFGRKGVAINFV  +D +ILRDIE +Y+T ++EMPMNVADLI
Sbjct:  189 LSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKEELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTVSALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI 407
[Close]
BLAST of DY257175 vs. ExPASy Swiss-Prot
Match: IF4A2_PONAB (Eukaryotic initiation factor 4A-II OS=Pongo abelii GN=EIF4A2 PE=2 SV=1)

HSP 1 Score: 339.732 bits (870), Expect = 1.481e-92
Identity = 163/219 (74.43%), Postives = 194/219 (88.58%), Query Frame = 2 
Query:    2 LSRGFKDQIYDVYRYLPPDLQVVLISATLPHEILEMTTKFMTDPVKILVKRDELTLEGIKQFFVAVEREEWKFDTLCDLYDTLTITQAVIFCNTKRKVDWLTEKMRGYNFTVSSMHGDMPQKERDAIMGEFRSGTTRVLITTDVWARGLDVQQVSLVINYDLPNNRELYIHRIGRSGRFGRKGVAINFVKNDDIKILRDIEQYYSTQIDEMPMNVADLI 658
            LSRGFKDQIY++++ L   +QVVL+SAT+P ++LE+T KFM DP++ILVK++ELTLEGIKQF++ VEREEWK DTLCDLY+TLTITQAVIF NT+RKVDWLTEKM   +FTVS++HGDM QKERD IM EFRSG++RVLITTD+ ARG+DVQQVSLVINYDLP NRE YIHRIGR GRFGRKGVAINFV  +D +ILRDIE +Y+T ++EMPMNVADLI
Sbjct:  189 LSRGFKDQIYEIFQKLNTSIQVVLLSATMPTDVLEVTKKFMRDPIRILVKKEELTLEGIKQFYINVEREEWKLDTLCDLYETLTITQAVIFLNTRRKVDWLTEKMHARDFTVSALHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINFVTEEDKRILRDIETFYNTTVEEMPMNVADLI 407
The following BLAST results are available for this feature:
BLAST of DY257175 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
IF4A_CHAGB2.285e-9373.97ATP-dependent RNA helicase eIF4A OS=Chaetomium glo... [more]
IF4A_PHANO2.984e-9373.97ATP-dependent RNA helicase eIF4A OS=Phaeosphaeria ... [more]
IF4A_NEUCR5.090e-9373.97ATP-dependent RNA helicase eIF4A OS=Neurospora cra... [more]
IF4A_ASPOR5.090e-9373.97ATP-dependent RNA helicase eIF4A OS=Aspergillus or... [more]
IF4A_ASPCL5.090e-9373.97ATP-dependent RNA helicase eIF4A OS=Aspergillus cl... [more]
IF4A_NEOFI6.648e-9373.97ATP-dependent RNA helicase eIF4A OS=Neosartorya fi... [more]
IF4A_EMENI6.648e-9373.97ATP-dependent RNA helicase eIF4A OS=Emericella nid... [more]
IF4A_ASPTN1.134e-9273.97ATP-dependent RNA helicase eIF4A OS=Aspergillus te... [more]
IF4A2_RAT1.481e-9274.43Eukaryotic initiation factor 4A-II OS=Rattus norve... [more]
IF4A2_PONAB1.481e-9274.43Eukaryotic initiation factor 4A-II OS=Pongo abelii... [more]

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Properties
Property NameValue
Genbank descriptionKN0AAK1BE01FM2 AbsCFruit Citrus sinensis cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY257175 ID=DY257175; Name=DY257175; organism=Citrus sinensis; type=EST; length=969bp
GCTGAGCAGAGGGTTCAAGGATCAAATTTATGATGTGTACCGTTATCTTC
CACCGGATCTTCAGGTTGTTTTGATATCTGCCACCCTTCCTCACGAAATA
TTGGAGATGACGACCAAATTCATGACAGATCCTGTAAAGATTCTTGTGAA
GCGTGATGAGTTGACTTTGGAGGGAATCAAGCAATTTTTTGTTGCAGTGG
AAAGAGAGGAGTGGAAGTTTGATACACTCTGTGATCTTTATGATACCCTT
ACGATTACACAAGCTGTTATTTTCTGCAACACGAAGCGTAAGGTGGATTG
GCTAACAGAGAAGATGCGCGGCTATAACTTTACTGTCTCATCAATGCATG
GAGACATGCCTCAGAAGGAAAGAGATGCAATTATGGGAGAATTTCGGTCT
GGGACAACCCGTGTGCTCATCACAACTGATGTTTGGGCACGAGGACTTGA
TGTTCAGCAGGTTTCTTTGGTGATAAATTATGACCTTCCTAACAATCGTG
AGCTTTATATTCACCGGATTGGTCGTTCTGGACGTTTTGGACGCAAGGGT
GTAGCAATAAATTTTGTCAAAAACGATGATATCAAGATTCTTAGAGATAT
CGAGCAATATTACAGTACCCAGATTGATGAAATGCCAATGAATGTTGCTG
ATCTGATATAAGAGATGTTTGTGATCTCGTCTGCCAGAGTGGAGGTTATG
GGATTGATCAATGAAAGGTTGAAGACAATTGTGTGATTGACAGTTTCTGC
AAGTGTGACATTGTCTTTGTTTTTGTGAGGAGTTTGTATAATACAGGATG
TTCTCATATTTTCTGTATGGTATTGATGACAAAAAAAAAAAAAGAGTAGG
CATTAACATTTGTGATAAAATTCTAGTGATTGCCTTTTTGCACCTTGACC
TGATTTTAAGGGTGGCGAATTGGTTCACGGTTGAATTCAAATTATTTTTA
ACCCTTTTTGAAAAAATTG
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