CN189604 | Citrus Genome Database

Overview


Name	CN189604
Unique Name	CN189604
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	769

Libraries


Library Name	Type
Washington Navel Orange Stored Fruit Rind cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig2496	contig	Csv1_Contig2496:8..777.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_XANOM (Homogentisate 1,2-dioxygenase OS=Xanthomonas oryzae pv. oryzae (strain MAFF 311018) GN=hmgA PE=3 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 1.452e-58
Identity = 76/134 (56.72%), Postives = 94/134 (70.15%), Query Frame = 2

Query:  362 PTQLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDF 763
            P Q+RW P+ +P +PTDF+DGLYT+ G GS     G A+H+Y AN SM    F NADG+ L+VPQ GRL + TE G LE+ P +I V+P+G RF V L D  +RGY+ E FG   +LPDLGPIGANGLA PRDF
Sbjct:   91 PDQMRWSPLPLPATPTDFVDGLYTMAGNGSPEAMTGVAVHLYAANASMHGRFFYNADGELLLVPQLGRLRVCTELGVLELEPQQIGVIPRGVRFRVELLDSAARGYVCENFGGLLRLPDLGPIGANGLANPRDF 224

HSP 2 Score: 87.8113 bits (216), Expect = 1.452e-58
Identity = 41/69 (59.42%), Postives = 53/69 (76.81%), Query Frame = 3

Query:   96 NYESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPSQHMNRLSL 302
            +Y +GFGN F++EA+ G+LP GQNSP     GLYAEQ+SGT+FT+PR  N+RSWLYRI+P+    R SL
Sbjct:    6 HYMTGFGNEFATEAVAGSLPVGQNSPQRVAHGLYAEQLSGTAFTAPRGENRRSWLYRIRPAAVHGRFSL 74

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_XANOR (Homogentisate 1,2-dioxygenase OS=Xanthomonas oryzae pv. oryzae GN=hmgA PE=3 SV=1)

HSP 1 Score: 159.844 bits (403), Expect = 1.893e-58
Identity = 75/134 (55.97%), Postives = 94/134 (70.15%), Query Frame = 2

Query:  362 PTQLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDF 763
            P Q+RW P+ +P +PTDF+DGLYT+ G GS     G A+H+Y AN SM    F NADG+ L+VPQ GRL + TE G LE+ P ++ V+P+G RF V L D  +RGY+ E FG   +LPDLGPIGANGLA PRDF
Sbjct:   91 PDQMRWSPLPLPATPTDFVDGLYTMAGNGSPEAMTGVAVHLYAANASMHGRFFYNADGELLLVPQLGRLRVCTELGVLELEPQQVGVIPRGVRFRVELLDSAARGYVCENFGGLLRLPDLGPIGANGLANPRDF 224

HSP 2 Score: 87.8113 bits (216), Expect = 1.893e-58
Identity = 41/69 (59.42%), Postives = 53/69 (76.81%), Query Frame = 3

Query:   96 NYESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPSQHMNRLSL 302
            +Y +GFGN F++EA+ G+LP GQNSP     GLYAEQ+SGT+FT+PR  N+RSWLYRI+P+    R SL
Sbjct:    6 HYMTGFGNEFATEAVAGSLPVGQNSPQRVAHGLYAEQLSGTAFTAPRGENRRSWLYRIRPAAVHGRFSL 74

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_XANC5 (Homogentisate 1,2-dioxygenase OS=Xanthomonas campestris pv. vesicatoria (strain 85-10) GN=hmgA PE=3 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 3.502e-57
Identity = 74/134 (55.22%), Postives = 94/134 (70.15%), Query Frame = 2

Query:  362 PTQLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDF 763
            P Q+RW P+ +P + TDF+DGLYT+ G GS     G A+H+Y AN SM +  F NADG+ L+VPQ GRL + TE G LE+ P +I V+P+G RF V L D  +RGY+ E FG   +LP+LGPIGANGLA PRDF
Sbjct:   93 PDQMRWSPLPLPATRTDFVDGLYTMAGNGSPEAMTGVAVHLYAANASMRDRFFYNADGELLLVPQLGRLRVCTELGVLELEPQQIGVIPRGLRFRVELLDSEARGYVCENFGGLLRLPELGPIGANGLANPRDF 226

HSP 2 Score: 87.8113 bits (216), Expect = 3.502e-57
Identity = 41/69 (59.42%), Postives = 53/69 (76.81%), Query Frame = 3

Query:   96 NYESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPSQHMNRLSL 302
            +Y +GFGN F++EA+ G+LP GQNSP     GLYAEQ+SGT+FT+PR  N+RSWLYRI+P+    R SL
Sbjct:    8 SYMTGFGNEFATEAVAGSLPVGQNSPQRVAHGLYAEQLSGTAFTAPRGENRRSWLYRIRPAAVHGRFSL 76

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_XANAC (Homogentisate 1,2-dioxygenase OS=Xanthomonas axonopodis pv. citri GN=hmgA PE=3 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 5.950e-57
Identity = 73/134 (54.48%), Postives = 92/134 (68.66%), Query Frame = 2

Query:  362 PTQLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDF 763
            P QLRW P+ +P  PTDF+DGLYT+ G G      G  +H+Y AN SM +  F +ADG+ L+VPQ+GRL + TE G L + P +I V+P+G RF V L D  +RGY+ E FG   +LPDLGPIGANGLA PRDF
Sbjct:  112 PDQLRWSPLPLPSVPTDFVDGLYTMAGNGGPEAMSGVGVHVYAANASMQDRFFYDADGELLLVPQQGRLRVHTELGVLALEPQQIGVIPRGMRFRVELLDAAARGYVCENFGGLLRLPDLGPIGANGLANPRDF 245

HSP 2 Score: 87.8113 bits (216), Expect = 5.950e-57
Identity = 42/77 (54.55%), Postives = 54/77 (70.13%), Query Frame = 3

Query:   72 DGEDFSDLNYESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPSQHMNRLSL 302
            D    +D  Y +GFGN F++EA+ G+LP GQNSP     GLYAEQ+SGT+FT+PR  N+RSWLYRI+P+      SL
Sbjct:   19 DAPMHNDQRYMTGFGNEFATEAVAGSLPIGQNSPQRVAHGLYAEQLSGTAFTAPRGQNRRSWLYRIRPAAVHGSFSL 95

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_PSEPW (Homogentisate 1,2-dioxygenase OS=Pseudomonas putida (strain W619) GN=hmgA PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 1.720e-56
Identity = 75/136 (55.15%), Postives = 97/136 (71.32%), Query Frame = 2

Query:  359 TPTQLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDFL 766
            TP +LRW P  +P  PTDFI+G   +    ++    G ++++Y AN+SM+   F NADG+ L+VP++GRL IATE G LEV P EIAV+P+G +F V LPDG +RGYIAE  G   +LPDLGPIG+NGLA PRDFL
Sbjct:   92 TPNRLRWNPQPLPTEPTDFIEGWLPMVANSAADKPAGVSVYIYCANRSMER-VFFNADGELLIVPEQGRLRIATELGLLEVEPQEIAVVPRGMKFRVELPDGQARGYIAENHGAPLRLPDLGPIGSNGLANPRDFL 226

HSP 2 Score: 87.8113 bits (216), Expect = 1.720e-56
Identity = 41/63 (65.08%), Postives = 49/63 (77.78%), Query Frame = 3

Query:   90 DLNYESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPS 278
            DL Y SGFGN F+SEA+ GALP GQNSP   P+GLYAE +SGT+FT  R   +R+WLYRI+PS
Sbjct:    8 DLQYLSGFGNEFASEALPGALPVGQNSPQKAPYGLYAELLSGTAFTMARSELRRTWLYRIRPS 70

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_PSEAE (Homogentisate 1,2-dioxygenase OS=Pseudomonas aeruginosa GN=hmgA PE=3 SV=2)

HSP 1 Score: 150.599 bits (379), Expect = 8.443e-56
Identity = 74/136 (54.41%), Postives = 95/136 (69.85%), Query Frame = 2

Query:  359 TPTQLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDFL 766
            TP +LRW P+ +P+ PTDF+DGL  +          G +I+ Y AN+SM+ C F +ADG+ L+VPQ GRL + TE G L+V P EIAV+P+G +F V L DG +RGYIAE  G   +LPDLGPIG+NGLA PRDFL
Sbjct:   91 TPNRLRWDPLALPEQPTDFLDGLLRMAANAPGDKPAGVSIYQYLANRSMERC-FYDADGELLLVPQLGRLRLCTELGALQVEPLEIAVIPRGMKFRVELLDGEARGYIAENHGAPLRLPDLGPIGSNGLANPRDFL 225

HSP 2 Score: 88.1965 bits (217), Expect = 8.443e-56
Identity = 42/66 (63.64%), Postives = 50/66 (75.76%), Query Frame = 3

Query:   81 DFSDLNYESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPS 278
            D + L Y+SGFGN FSSEA+ GALP GQNSP   P+GLYAE +SGT+FT  R   +R+WLYRI PS
Sbjct:    4 DSTALAYQSGFGNEFSSEALPGALPVGQNSPQKAPYGLYAELLSGTAFTMARSEARRTWLYRITPS 69

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_PSEAB (Homogentisate 1,2-dioxygenase OS=Pseudomonas aeruginosa (strain UCBPP-PA14) GN=hmgA PE=3 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 8.443e-56
Identity = 74/136 (54.41%), Postives = 95/136 (69.85%), Query Frame = 2

Query:  359 TPTQLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDFL 766
            TP +LRW P+ +P+ PTDF+DGL  +          G +I+ Y AN+SM+ C F +ADG+ L+VPQ GRL + TE G L+V P EIAV+P+G +F V L DG +RGYIAE  G   +LPDLGPIG+NGLA PRDFL
Sbjct:   91 TPNRLRWDPLALPEQPTDFLDGLLRMAANAPGDKPAGVSIYQYLANRSMERC-FYDADGELLLVPQLGRLRLCTELGALQVEPLEIAVIPRGMKFRVELLDGEARGYIAENHGAPLRLPDLGPIGSNGLANPRDFL 225

HSP 2 Score: 88.1965 bits (217), Expect = 8.443e-56
Identity = 42/66 (63.64%), Postives = 50/66 (75.76%), Query Frame = 3

Query:   81 DFSDLNYESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPS 278
            D + L Y+SGFGN FSSEA+ GALP GQNSP   P+GLYAE +SGT+FT  R   +R+WLYRI PS
Sbjct:    4 DSTALAYQSGFGNEFSSEALPGALPVGQNSPQKAPYGLYAELLSGTAFTMARSEARRTWLYRITPS 69

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_PSEA8 (Homogentisate 1,2-dioxygenase OS=Pseudomonas aeruginosa (strain LESB58) GN=hmgA PE=3 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 8.443e-56
Identity = 74/136 (54.41%), Postives = 95/136 (69.85%), Query Frame = 2

Query:  359 TPTQLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDFL 766
            TP +LRW P+ +P+ PTDF+DGL  +          G +I+ Y AN+SM+ C F +ADG+ L+VPQ GRL + TE G L+V P EIAV+P+G +F V L DG +RGYIAE  G   +LPDLGPIG+NGLA PRDFL
Sbjct:   91 TPNRLRWDPLALPEQPTDFLDGLLRMAANAPGDKPAGVSIYQYLANRSMERC-FYDADGELLLVPQLGRLRLCTELGALQVEPLEIAVIPRGMKFRVELLDGEARGYIAENHGAPLRLPDLGPIGSNGLANPRDFL 225

HSP 2 Score: 88.1965 bits (217), Expect = 8.443e-56
Identity = 42/66 (63.64%), Postives = 50/66 (75.76%), Query Frame = 3

Query:   81 DFSDLNYESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPS 278
            D + L Y+SGFGN FSSEA+ GALP GQNSP   P+GLYAE +SGT+FT  R   +R+WLYRI PS
Sbjct:    4 DSTALAYQSGFGNEFSSEALPGALPVGQNSPQKAPYGLYAELLSGTAFTMARSEARRTWLYRITPS 69

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_PSEA7 (Homogentisate 1,2-dioxygenase OS=Pseudomonas aeruginosa (strain PA7) GN=hmgA PE=3 SV=1)

HSP 1 Score: 150.599 bits (379), Expect = 8.443e-56
Identity = 74/136 (54.41%), Postives = 95/136 (69.85%), Query Frame = 2

Query:  359 TPTQLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDFL 766
            TP +LRW P+ +P+ PTDF+DGL  +          G +I+ Y AN+SM+ C F +ADG+ L+VPQ GRL + TE G L+V P EIAV+P+G +F V L DG +RGYIAE  G   +LPDLGPIG+NGLA PRDFL
Sbjct:   91 TPNRLRWDPLALPEQPTDFLDGLLRMAANAPGDKPAGVSIYQYLANRSMERC-FYDADGELLLVPQLGRLRLCTELGALQVEPLEIAVIPRGMKFRVELLDGEARGYIAENHGAPLRLPDLGPIGSNGLANPRDFL 225

HSP 2 Score: 88.1965 bits (217), Expect = 8.443e-56
Identity = 42/66 (63.64%), Postives = 50/66 (75.76%), Query Frame = 3

Query:   81 DFSDLNYESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPS 278
            D + L Y+SGFGN FSSEA+ GALP GQNSP   P+GLYAE +SGT+FT  R   +R+WLYRI PS
Sbjct:    4 DSTALAYQSGFGNEFSSEALPGALPVGQNSPQKAPYGLYAELLSGTAFTMARSEARRTWLYRITPS 69

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_PSEPF (Homogentisate 1,2-dioxygenase OS=Pseudomonas fluorescens (strain Pf0-1) GN=hmgA PE=3 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 8.444e-56
Identity = 77/136 (56.62%), Postives = 97/136 (71.32%), Query Frame = 2

Query:  359 TPTQLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDFL 766
            TP +LRW P+D+P  PTDFIDGL ++     +    G +I+ Y AN+SM+   F NADG+ L+VPQ GRL IATE G L+V+P EIAVLP+G +F V L D  +RGY+AE  G   +LPDLGPIG+NGLA PRDFL
Sbjct:   90 TPNRLRWNPLDLPTEPTDFIDGLVSMAANSGADKPAGISIYHYGANRSMER-VFFNADGELLLVPQLGRLRIATELGVLDVAPLEIAVLPRGLKFRVELLDPQARGYVAENHGAPLRLPDLGPIGSNGLANPRDFL 224

HSP 2 Score: 86.6557 bits (213), Expect = 8.444e-56
Identity = 45/79 (56.96%), Postives = 54/79 (68.35%), Query Frame = 3

Query:   87 SDLNYESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPS-QHMNRLSLECLLMG 320
            S L Y+SGFGN FSSEA+ GALP GQNSP   P+GLY E  SGT+FT  R   +R+W+YRI+PS  H   + LE  L G
Sbjct:    5 SALAYQSGFGNEFSSEALPGALPVGQNSPQKAPYGLYTELFSGTAFTMTRSEARRTWMYRIQPSANHPAFVKLERQLAG 83

The following BLAST results are available for this feature:

BLAST of CN189604 vs. ExPASy Swiss-Prot

BLAST of CN189604 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 83


Match Name	E-value	Identity	Description
HGD_XANOM	1.452e-58	56.72	Homogentisate 1,2-dioxygenase OS=Xanthomonas oryza...	[more]
HGD_XANOR	1.893e-58	55.97	Homogentisate 1,2-dioxygenase OS=Xanthomonas oryza...	[more]
HGD_XANC5	3.502e-57	55.22	Homogentisate 1,2-dioxygenase OS=Xanthomonas campe...	[more]
HGD_XANAC	5.950e-57	54.48	Homogentisate 1,2-dioxygenase OS=Xanthomonas axono...	[more]
HGD_PSEPW	1.720e-56	55.15	Homogentisate 1,2-dioxygenase OS=Pseudomonas putid...	[more]
HGD_PSEAE	8.443e-56	54.41	Homogentisate 1,2-dioxygenase OS=Pseudomonas aerug...	[more]
HGD_PSEAB	8.443e-56	54.41	Homogentisate 1,2-dioxygenase OS=Pseudomonas aerug...	[more]
HGD_PSEA8	8.443e-56	54.41	Homogentisate 1,2-dioxygenase OS=Pseudomonas aerug...	[more]
HGD_PSEA7	8.443e-56	54.41	Homogentisate 1,2-dioxygenase OS=Pseudomonas aerug...	[more]
HGD_PSEPF	8.444e-56	56.62	Homogentisate 1,2-dioxygenase OS=Pseudomonas fluor...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS06_0002C02_r Washington Navel Orange Stored Fruit Rind cDNA Library Citrus sinensis cDNA clone CS_WEc0002C02, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN189604 ID=CN189604; Name=CN189604; organism=Citrus sinensis; type=EST; length=769bp
GAGGGTGAGCTGCTGAGACACAAATGGAGAAGAAGAGTCAACATCAATCA
TCATCATTAATCAGAAAAACGGACGGCGAAGATTTCTCTGATCTGAATTA
CGAATCAGGATTCGGCAACAGCTTCTCGTCGGAGGCGATCGACGGTGCGT
TGCCACGTGGACAGAACAGCCCCCTCGTTTGTCCGTTCGGTCTCTACGCC
GAACAGATCTCCGGCACCTCCTTCACCTCCCCTCGCAAGCTCAATCAACG
CAGTTGGTTATACAGAATAAAACCTTCTCAACACATGAACCGTTTAAGCC
TCGAGTGCCTGCTCATGGGAAGCTGGTGAGTGAATTTGACAAGTCTAACA
GTTACACTACGCCGACTCAGCTCCGGTGGAAGCCGGTTGATATCCCTGAT
TCACCGACTGATTTTATTGACGGGTTGTACACGATATGTGGGGCTGGCAG
CTCATTTCTTCGGCATGGATATGCTATTCACATGTACACTGCCAACAAAT
CAATGGACAACTGTGCCTTCTGTAATGCTGATGGAGATTTTTTGGTAGTT
CCCCAAAAAGGAAGGCTGTGGATTGCTACTGAATGTGGAAAACTGGAAGT
CTCTCCTGGTGAAATTGCCGTTTTACCTCAAGGGTTTCGTTTTGCTGTAA
GTTTACCAGATGGTCCATCACGTGGTTACATCGCAGAGATTTTTGGTACC
CATTTTCAGCTTCCTGATCTTGGTCCAATAGGTGCAAATGGTCTTGCTGC
TCCGAGAGATTTCCTTGTT