CN189604 | Citrus Genome Database

Overview


Name	CN189604
Unique Name	CN189604
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	769

Libraries


Library Name	Type
Washington Navel Orange Stored Fruit Rind cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig2496	contig	Csv1_Contig2496:8..777.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_EMENI (Homogentisate 1,2-dioxygenase OS=Emericella nidulans GN=hmgA PE=2 SV=1)

HSP 1 Score: 147.517 bits (371), Expect = 4.084e-52
Identity = 78/160 (48.75%), Postives = 101/160 (63.12%), Query Frame = 2

Query:  311 AHGKLVSEFDKSNSYTT---------PTQLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDF 763
            AH   V E D S+ +T          P QLRW P D+ D   D++ GL+ + G+G   ++ G  I +Y A K M   AF +ADGDFL+V Q G L I TE G+L V P EI V+P+G R+ V+LPDGP RGYI E++  H+QLP+LGPIG+NGLA  RDF
Sbjct:   75 AHENFVEE-DASSYHTLSDAKKLQHIPNQLRWDPFDL-DETVDWVHGLHLVAGSGDPTVKQGLGILLYAAGKDMGKEAFYSADGDFLIVAQHGVLDIQTELGRLLVRPNEICVIPRGVRYRVTLPDGPVRGYICELYQGHYQLPELGPIGSNGLANARDF 232

HSP 2 Score: 78.9518 bits (193), Expect = 4.084e-52
Identity = 34/60 (56.67%), Postives = 48/60 (80.00%), Query Frame = 3

Query:   99 YESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPS 278
            Y++GF +   SEAI+GALP G NSP   P+GLYAE++SGT+FT+PR  N+++W+YRI P+
Sbjct:   14 YQNGFDSYHESEAIEGALPVGHNSPQKAPYGLYAEKLSGTAFTAPRHENKQTWVYRILPA 73

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_RHOPB (Homogentisate 1,2-dioxygenase OS=Rhodopseudomonas palustris (strain BisB18) GN=hmgA PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 9.045e-52
Identity = 67/133 (50.38%), Postives = 86/133 (64.66%), Query Frame = 2

Query:  368 QLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDFL 766
            QLRW P  +P     F+ G+ T+  AG    + G A HMY  + SM N  F NADG+ + VPQ+G L + TE G + V+PGEIAV+P+G +F V L DGP+RGY+ E +G  F LP+ GPIGAN LA  RDFL
Sbjct:  109 QLRWDPPPLPQRAQTFLQGVETMTTAGDVNTQAGMAAHMYLISASMVNQHFYNADGELMFVPQQGGLRLVTEFGVIGVAPGEIAVIPRGVKFRVELIDGPARGYLCENYGGGFTLPERGPIGANCLANARDFL 241

HSP 2 Score: 93.2041 bits (230), Expect = 9.045e-52
Identity = 44/66 (66.67%), Postives = 53/66 (80.30%), Query Frame = 3

Query:   99 YESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPS-QHMNR 293
            Y SGFGNSF +EA+ GALP G+NSP   P+GLYAEQ+SG+ FT+PR  N+RSWLYRI+PS QH  R
Sbjct:   21 YMSGFGNSFETEALPGALPIGRNSPQRAPYGLYAEQLSGSPFTAPRGSNERSWLYRIRPSVQHSGR 86

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_RHILO (Homogentisate 1,2-dioxygenase OS=Rhizobium loti GN=hmgA PE=3 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 2.003e-51
Identity = 71/132 (53.79%), Postives = 84/132 (63.64%), Query Frame = 2

Query:  368 QLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDF 763
            Q RW PV +P  PTDFI G+ +I  AG    + G A H+Y AN+SM +  F NADG+ LVVPQ G L   TE G +E+ PGEIAVLP+G  F   L D   RGY+ E +G    LPD GPIGAN LA PRDF
Sbjct:  114 QYRWNPVPMPKEPTDFIQGMRSITTAGDVLGQTGMAAHVYVANRSMVDDHFFNADGELLVVPQVGALRFVTEMGVIELRPGEIAVLPRGLVFKAELVDKDVRGYVCENYGAKLTLPDRGPIGANCLANPRDF 245

HSP 2 Score: 85.5001 bits (210), Expect = 2.003e-51
Identity = 40/73 (54.79%), Postives = 53/73 (72.60%), Query Frame = 3

Query:   78 EDFSDLNYESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPS-QHMNR 293
            ED    +Y  GFGN F +E + G+LP+G+NSP    +GLYAEQ+SG+ FT+PR  N+RSWLYRI+PS +H  R
Sbjct:   18 EDRMAFSYMPGFGNDFETETLPGSLPQGRNSPQRPAYGLYAEQLSGSPFTAPRGTNERSWLYRIRPSVKHTGR 90

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_NITHX (Homogentisate 1,2-dioxygenase OS=Nitrobacter hamburgensis (strain X14 / DSM 10229) GN=hmgA PE=3 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 2.611e-51
Identity = 64/133 (48.12%), Postives = 84/133 (63.16%), Query Frame = 2

Query:  368 QLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDFL 766
            QLRW P  IP+    F+  + T+  AG +  + G A H+Y   +SM +  F NADG+ L VPQ G L   TE G ++  PGEIAV+P+G +F V +P GP+RGY+ E +G  F LP+ GPIGAN LA  RDFL
Sbjct:  109 QLRWDPTPIPEDELTFVQSVRTMTTAGDAHTQTGMAAHIYLITRSMVDQHFYNADGEMLFVPQGGSLRFVTEFGVIDTEPGEIAVIPRGVKFRVEIPSGPARGYLCENYGGAFTLPERGPIGANCLANSRDFL 241

HSP 2 Score: 92.0485 bits (227), Expect = 2.611e-51
Identity = 41/60 (68.33%), Postives = 50/60 (83.33%), Query Frame = 3

Query:   99 YESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPS 278
            Y SGFGNSF +EA+ GALP G+NSP  C +GLYAEQ+SG+ FT+PR  N+RSWLYRI+PS
Sbjct:   21 YMSGFGNSFETEALPGALPIGRNSPQRCAYGLYAEQLSGSPFTAPRGANERSWLYRIRPS 80

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_BRAJA (Homogentisate 1,2-dioxygenase OS=Bradyrhizobium japonicum GN=hmgA PE=3 SV=1)

HSP 1 Score: 130.183 bits (326), Expect = 5.783e-51
Identity = 63/141 (44.68%), Postives = 88/141 (62.41%), Query Frame = 2

Query:  347 NSYTTP-TQLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDFL 766
            + Y  P  Q+RW P  +P     F+ G+ T+  AG    + G A H+Y   KSM +  F NADG+ + V Q+G L + TE G+++  PGEI V+P+G +F V +P+GP+RGY+ E +G  F LP+ GPIGAN LA  RDFL
Sbjct:  101 HEYDLPIAQMRWDPTPVPKEEVTFVQGVQTMTTAGDVNTQAGMAAHVYLITKSMVDQHFYNADGELMFVLQQGNLRLVTEFGRIDAEPGEIVVIPRGVKFRVEIPNGPARGYLCENYGGAFTLPERGPIGANCLANARDFL 241

HSP 2 Score: 92.4337 bits (228), Expect = 5.783e-51
Identity = 43/66 (65.15%), Postives = 53/66 (80.30%), Query Frame = 3

Query:   99 YESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPS-QHMNR 293
            Y SGFGNSF +EA+ GALP G+NSP  C +GLYAEQ+SG+ FT+PR  N+RSWLYRI+PS +H  R
Sbjct:   21 YMSGFGNSFETEALPGALPIGRNSPQRCAYGLYAEQLSGSPFTAPRGTNERSWLYRIRPSVKHSGR 86

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_RHOPT (Homogentisate 1,2-dioxygenase OS=Rhodopseudomonas palustris (strain TIE-1) GN=hmgA PE=3 SV=1)

HSP 1 Score: 127.487 bits (319), Expect = 2.176e-50
Identity = 63/133 (47.37%), Postives = 84/133 (63.16%), Query Frame = 2

Query:  368 QLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDFL 766
            QLRW P  +P     F+ G+ T+  AG    + G A H+Y   +SM +  F NADG+ + VPQ+G L + TE G + + P EIAV+P+G +F V L DGP+RGY+ E +G  F LP+ GPIGAN LA  RDFL
Sbjct:  109 QLRWDPPPMPQEKLTFLQGVRTMTTAGDVNTQAGMATHLYLITQSMVDQHFYNADGEMMFVPQQGSLRLVTEFGIITIEPAEIAVIPRGIKFRVELVDGPARGYLCENYGGAFTLPERGPIGANCLANSRDFL 241

HSP 2 Score: 93.2041 bits (230), Expect = 2.176e-50
Identity = 46/77 (59.74%), Postives = 57/77 (74.03%), Query Frame = 3

Query:   99 YESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPS-QHMNRLSLECLLMGSW 326
            Y SGFGNSF +EA+ GALP G+NSP  C +GLYAEQ+SG+ FT+PR  N+RSWLYRI+PS +H  R +     MG W
Sbjct:   21 YMSGFGNSFETEALPGALPVGRNSPQRCAYGLYAEQLSGSPFTAPRGANERSWLYRIRPSVKHSGRFAKTD--MGLW 95

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_RHOPA (Homogentisate 1,2-dioxygenase OS=Rhodopseudomonas palustris GN=hmgA PE=3 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 2.837e-50
Identity = 63/133 (47.37%), Postives = 84/133 (63.16%), Query Frame = 2

Query:  368 QLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDFL 766
            QLRW P  +P     F+ G+ T+  AG    + G A H+Y   +SM +  F NADG+ + VPQ+G L + TE G + + P EIAV+P+G +F V L DGP+RGY+ E +G  F LP+ GPIGAN LA  RDFL
Sbjct:  109 QLRWDPPPMPQEALTFLQGVRTMTTAGDVNTQAGMATHLYLITQSMVDQHFYNADGEMMFVPQQGSLRLVTEFGIITIEPAEIAVIPRGVKFRVELVDGPARGYLCENYGGAFTLPERGPIGANCLANSRDFL 241

HSP 2 Score: 93.2041 bits (230), Expect = 2.837e-50
Identity = 46/77 (59.74%), Postives = 57/77 (74.03%), Query Frame = 3

Query:   99 YESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPS-QHMNRLSLECLLMGSW 326
            Y SGFGNSF +EA+ GALP G+NSP  C +GLYAEQ+SG+ FT+PR  N+RSWLYRI+PS +H  R +     MG W
Sbjct:   21 YMSGFGNSFETEALPGALPVGRNSPQRCAYGLYAEQLSGSPFTAPRGANERSWLYRIRPSVKHSGRFAKTD--MGLW 95

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_RHOP5 (Homogentisate 1,2-dioxygenase OS=Rhodopseudomonas palustris (strain BisA53) GN=hmgA PE=3 SV=1)

HSP 1 Score: 129.413 bits (324), Expect = 8.187e-50
Identity = 63/133 (47.37%), Postives = 84/133 (63.16%), Query Frame = 2

Query:  368 QLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDFL 766
            QLRW P  +P     F+ G+ T+  AG +  + G A H+Y    SM + AF NADG+ + VPQ+G L   TE G+++  PGEI V+P+G +F V L  GP+RGY+ E +G  F LP+ GPIGAN LA  RDFL
Sbjct:  109 QLRWDPPPLPQHEQTFLQGVVTMTTAGDANTQAGMAAHIYLITASMVDQAFYNADGELMFVPQQGSLRFVTEFGRIDAGPGEIVVIPRGVKFRVELTGGPARGYLCENYGGAFTLPERGPIGANCLANARDFL 241

HSP 2 Score: 89.3521 bits (220), Expect = 8.187e-50
Identity = 42/68 (61.76%), Postives = 53/68 (77.94%), Query Frame = 3

Query:   99 YESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPS-QHMNRLS 299
            Y SGF NSF +EA+ GALP G+NSP  C +GLYAEQ+SG+ FT+PR  N+RSWLYRI+PS +H  R +
Sbjct:   21 YMSGFKNSFETEALPGALPIGRNSPQRCAYGLYAEQLSGSPFTAPRGSNERSWLYRIRPSVKHSGRFA 88

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_RHOPS (Homogentisate 1,2-dioxygenase OS=Rhodopseudomonas palustris (strain BisB5) GN=hmgA PE=3 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 8.187e-50
Identity = 64/133 (48.12%), Postives = 85/133 (63.91%), Query Frame = 2

Query:  368 QLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDFL 766
            QLRW    +P     F+ G+ T+  AG    + G A HMY  ++SM +  F NADG+ + VPQ+GRL + TE G + + P EIAV+P+G +F V L DGP+RGY+ E +G  F LP+ GPIGAN LA  RDFL
Sbjct:  109 QLRWDAPPMPTEEVTFVQGVRTMTTAGDVNTQAGMAAHMYLISRSMVDQHFYNADGELMFVPQQGRLRLVTEFGVIAIEPAEIAVIPRGVKFRVELVDGPARGYLCENYGGAFTLPERGPIGANCLANSRDFL 241

HSP 2 Score: 89.7373 bits (221), Expect = 8.187e-50
Identity = 45/77 (58.44%), Postives = 56/77 (72.73%), Query Frame = 3

Query:   99 YESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPS-QHMNRLSLECLLMGSW 326
            Y SGFGNSF +EA+ GALP G+NSP    +GLYAEQ+SG+ FT+PR  N+RSWLYRI+PS +H  R +     MG W
Sbjct:   21 YMSGFGNSFETEALPGALPVGRNSPQRAAYGLYAEQLSGSPFTAPRGANERSWLYRIRPSVKHSGRFAKAD--MGLW 95

[Close]

BLAST of CN189604 vs. ExPASy Swiss-Prot
Match: HGD_RHOP2 (Homogentisate 1,2-dioxygenase OS=Rhodopseudomonas palustris (strain HaA2) GN=hmgA PE=3 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 5.233e-49
Identity = 63/133 (47.37%), Postives = 83/133 (62.41%), Query Frame = 2

Query:  368 QLRWKPVDIPDSPTDFIDGLYTICGAGSSFLRHGYAIHMYTANKSMDNCAFCNADGDFLVVPQKGRLWIATECGKLEVSPGEIAVLPQGFRFAVSLPDGPSRGYIAEIFGTHFQLPDLGPIGANGLAAPRDFL 766
            QLRW    +P     F+ G+ T+  AG    + G A HMY   +SM +  F NADG+ + VPQ+G L + TE G + + P EIAV+P+G +F V L DGP+RGY+ E +G  F LP+ GPIGAN LA  RDFL
Sbjct:  109 QLRWDAPSMPQEDLTFLQGVRTMTTAGDVNTQAGMATHMYLITQSMVDQHFYNADGELMFVPQQGSLRLVTEFGVISIEPAEIAVIPRGVKFRVELVDGPARGYLCENYGGAFTLPERGPIGANCLANSRDFL 241

HSP 2 Score: 89.7373 bits (221), Expect = 5.233e-49
Identity = 45/77 (58.44%), Postives = 56/77 (72.73%), Query Frame = 3

Query:   99 YESGFGNSFSSEAIDGALPRGQNSPLVCPFGLYAEQISGTSFTSPRKLNQRSWLYRIKPS-QHMNRLSLECLLMGSW 326
            Y SGFGNSF +EA+ GALP G+NSP    +GLYAEQ+SG+ FT+PR  N+RSWLYRI+PS +H  R +     MG W
Sbjct:   21 YMSGFGNSFETEALPGALPIGRNSPQRAAYGLYAEQLSGSPFTAPRGANERSWLYRIRPSVKHSGRFTKAD--MGLW 95

The following BLAST results are available for this feature:

BLAST of CN189604 vs. ExPASy Swiss-Prot

BLAST of CN189604 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 83


Match Name	E-value	Identity	Description
HGD_EMENI	4.084e-52	48.75	Homogentisate 1,2-dioxygenase OS=Emericella nidula...	[more]
HGD_RHOPB	9.045e-52	50.38	Homogentisate 1,2-dioxygenase OS=Rhodopseudomonas ...	[more]
HGD_RHILO	2.003e-51	53.79	Homogentisate 1,2-dioxygenase OS=Rhizobium loti GN...	[more]
HGD_NITHX	2.611e-51	48.12	Homogentisate 1,2-dioxygenase OS=Nitrobacter hambu...	[more]
HGD_BRAJA	5.783e-51	44.68	Homogentisate 1,2-dioxygenase OS=Bradyrhizobium ja...	[more]
HGD_RHOPT	2.176e-50	47.37	Homogentisate 1,2-dioxygenase OS=Rhodopseudomonas ...	[more]
HGD_RHOPA	2.837e-50	47.37	Homogentisate 1,2-dioxygenase OS=Rhodopseudomonas ...	[more]
HGD_RHOP5	8.187e-50	47.37	Homogentisate 1,2-dioxygenase OS=Rhodopseudomonas ...	[more]
HGD_RHOPS	8.187e-50	48.12	Homogentisate 1,2-dioxygenase OS=Rhodopseudomonas ...	[more]
HGD_RHOP2	5.233e-49	47.37	Homogentisate 1,2-dioxygenase OS=Rhodopseudomonas ...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS06_0002C02_r Washington Navel Orange Stored Fruit Rind cDNA Library Citrus sinensis cDNA clone CS_WEc0002C02, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN189604 ID=CN189604; Name=CN189604; organism=Citrus sinensis; type=EST; length=769bp
GAGGGTGAGCTGCTGAGACACAAATGGAGAAGAAGAGTCAACATCAATCA
TCATCATTAATCAGAAAAACGGACGGCGAAGATTTCTCTGATCTGAATTA
CGAATCAGGATTCGGCAACAGCTTCTCGTCGGAGGCGATCGACGGTGCGT
TGCCACGTGGACAGAACAGCCCCCTCGTTTGTCCGTTCGGTCTCTACGCC
GAACAGATCTCCGGCACCTCCTTCACCTCCCCTCGCAAGCTCAATCAACG
CAGTTGGTTATACAGAATAAAACCTTCTCAACACATGAACCGTTTAAGCC
TCGAGTGCCTGCTCATGGGAAGCTGGTGAGTGAATTTGACAAGTCTAACA
GTTACACTACGCCGACTCAGCTCCGGTGGAAGCCGGTTGATATCCCTGAT
TCACCGACTGATTTTATTGACGGGTTGTACACGATATGTGGGGCTGGCAG
CTCATTTCTTCGGCATGGATATGCTATTCACATGTACACTGCCAACAAAT
CAATGGACAACTGTGCCTTCTGTAATGCTGATGGAGATTTTTTGGTAGTT
CCCCAAAAAGGAAGGCTGTGGATTGCTACTGAATGTGGAAAACTGGAAGT
CTCTCCTGGTGAAATTGCCGTTTTACCTCAAGGGTTTCGTTTTGCTGTAA
GTTTACCAGATGGTCCATCACGTGGTTACATCGCAGAGATTTTTGGTACC
CATTTTCAGCTTCCTGATCTTGGTCCAATAGGTGCAAATGGTCTTGCTGC
TCCGAGAGATTTCCTTGTT