DR908756 | Citrus Genome Database

Overview


Name	DR908756
Unique Name	DR908756
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	584

Libraries


Library Name	Type
Citrus sinensis phloem	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_YERPS (Methylthioribose kinase OS=Yersinia pseudotuberculosis GN=mtnK PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.455e-27
Identity = 55/147 (37.41%), Postives = 83/147 (56.46%), Query Frame = 1

Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            +A  L  H Q CP H  +V H D  ++++    L   HI  R  LI G  YP  AE + E++A+TLF+TS  Y++    K  V+ +  N ELC++TE + F+DPY   + N++  P L  +  ++R+D  LKL VA LK  F  +A+
Sbjct:   80 EAETLLTHSQFCPQHTVKVLHHDAELAVMVQEDLSDHHI-WRHELIQGNYYPQAAEQLGEYLAQTLFHTSDFYQSAQAKKAAVSRYT-NPELCQITEDLFFTDPYIDHERNNF-DPVLLPEVLSLRQDKALKLAVASLKHRFLSQAE 223

HSP 2 Score: 46.595 bits (109), Expect = 2.455e-27
Identity = 17/30 (56.67%), Postives = 25/30 (83.33%), Query Frame = 3

Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSI 155
            ++KQALPYVRC+GESWP+T +RA +   ++
Sbjct:   55 IVKQALPYVRCVGESWPLTLDRARIEAETL 84

HSP 3 Score: 23.483 bits (49), Expect = 2.455e-27
Identity = 8/11 (72.73%), Postives = 10/11 (90.91%), Query Frame = 2

Query:    5 EVGDGNLNFVY 37
            E+GDGNLN V+
Sbjct:   33 EIGDGNLNLVF 43

[Close]

BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_YERPB (Methylthioribose kinase OS=Yersinia pseudotuberculosis serotype IB (strain PB1/+) GN=mtnK PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.455e-27
Identity = 55/147 (37.41%), Postives = 83/147 (56.46%), Query Frame = 1

Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            +A  L  H Q CP H  +V H D  ++++    L   HI  R  LI G  YP  AE + E++A+TLF+TS  Y++    K  V+ +  N ELC++TE + F+DPY   + N++  P L  +  ++R+D  LKL VA LK  F  +A+
Sbjct:   80 EAETLLTHSQFCPQHTVKVLHHDAELAVMVQEDLSDHHI-WRHELIQGNYYPQAAEQLGEYLAQTLFHTSDFYQSAQAKKAAVSRYT-NPELCQITEDLFFTDPYIDHERNNF-DPVLLPEVLSLRQDKALKLAVASLKHRFLSQAE 223

HSP 2 Score: 46.595 bits (109), Expect = 2.455e-27
Identity = 17/30 (56.67%), Postives = 25/30 (83.33%), Query Frame = 3

Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSI 155
            ++KQALPYVRC+GESWP+T +RA +   ++
Sbjct:   55 IVKQALPYVRCVGESWPLTLDRARIEAETL 84

HSP 3 Score: 23.483 bits (49), Expect = 2.455e-27
Identity = 8/11 (72.73%), Postives = 10/11 (90.91%), Query Frame = 2

Query:    5 EVGDGNLNFVY 37
            E+GDGNLN V+
Sbjct:   33 EIGDGNLNLVF 43

[Close]

BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_YERP3 (Methylthioribose kinase OS=Yersinia pseudotuberculosis serotype O:1b (strain IP 31758) GN=mtnK PE=3 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 2.455e-27
Identity = 55/147 (37.41%), Postives = 83/147 (56.46%), Query Frame = 1

Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            +A  L  H Q CP H  +V H D  ++++    L   HI  R  LI G  YP  AE + E++A+TLF+TS  Y++    K  V+ +  N ELC++TE + F+DPY   + N++  P L  +  ++R+D  LKL VA LK  F  +A+
Sbjct:   80 EAETLLTHSQFCPQHTVKVLHHDAELAVMVQEDLSDHHI-WRHELIQGNYYPQAAEQLGEYLAQTLFHTSDFYQSAQAKKAAVSRYT-NPELCQITEDLFFTDPYIDHERNNF-DPVLLPEVLSLRQDKALKLAVASLKHRFLSQAE 223

HSP 2 Score: 46.595 bits (109), Expect = 2.455e-27
Identity = 17/30 (56.67%), Postives = 25/30 (83.33%), Query Frame = 3

Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSI 155
            ++KQALPYVRC+GESWP+T +RA +   ++
Sbjct:   55 IVKQALPYVRCVGESWPLTLDRARIEAETL 84

HSP 3 Score: 23.483 bits (49), Expect = 2.455e-27
Identity = 8/11 (72.73%), Postives = 10/11 (90.91%), Query Frame = 2

Query:    5 EVGDGNLNFVY 37
            E+GDGNLN V+
Sbjct:   33 EIGDGNLNLVF 43

[Close]

BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_ERWCT (Methylthioribose kinase OS=Erwinia carotovora subsp. atroseptica GN=mtnK PE=3 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 4.138e-27
Identity = 54/147 (36.73%), Postives = 80/147 (54.42%), Query Frame = 1

Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            +A  L  H + CP H   V + D  ++++    L   H I R  L+ G  YP  A  + E++A+TLF+TS  Y+   E K  V++F  N ELC +TE + F+DPY   + N + +  L  D   +R+D  LKL VAGLK  F  +A+
Sbjct:   80 EAETLLTHARFCPQHTVTVLYHDPELAVMVQEDLSD-HRIWRSELVRGADYPQAAAQLGEYLAQTLFHTSDFYQHPHEKKAAVSQFT-NPELCEITEDLFFTDPYIDHERNQFDAA-LTPDVQTLRDDRALKLAVAGLKHRFLSKAE 223

HSP 2 Score: 46.595 bits (109), Expect = 4.138e-27
Identity = 17/30 (56.67%), Postives = 25/30 (83.33%), Query Frame = 3

Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSI 155
            ++KQALPYVRC+GESWP+T +RA +   ++
Sbjct:   55 IVKQALPYVRCVGESWPLTLDRARIEAETL 84

HSP 3 Score: 23.8682 bits (50), Expect = 4.138e-27
Identity = 8/12 (66.67%), Postives = 11/12 (91.67%), Query Frame = 2

Query:    2 KEVGDGNLNFVY 37
            +E+GDGNLN V+
Sbjct:   32 EEIGDGNLNLVF 43

[Close]

BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_PECCP (Methylthioribose kinase OS=Pectobacterium carotovorum subsp. carotovorum (strain PC1) GN=mtnK PE=3 SV=1)

HSP 1 Score: 91.2781 bits (225), Expect = 5.371e-27
Identity = 54/147 (36.73%), Postives = 82/147 (55.78%), Query Frame = 1

Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            +A  L  H + CP H   V + D  ++++    L   H I R  L+ G  YP  A  + E++A+TLF+TS  ++   E K  V++F  N ELC++TE + F+DPY   + N + +  L  D  A+R+D  LKL VAGLK  F  +A+
Sbjct:   80 EAETLLTHARFCPQHTVTVLYHDPELAVMVQEDLSD-HRIWRSELVKGADYPQAAAQLGEYLAQTLFHTSDFHQHPHEKKAAVSQFT-NPELCQITEDLFFTDPYIEHERNQFDAA-LTPDVQALRDDKALKLAVAGLKHGFLSKAE 223

HSP 2 Score: 46.595 bits (109), Expect = 5.371e-27
Identity = 17/30 (56.67%), Postives = 25/30 (83.33%), Query Frame = 3

Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSI 155
            ++KQALPYVRC+GESWP+T +RA +   ++
Sbjct:   55 IVKQALPYVRCVGESWPLTLDRARIEAETL 84

HSP 3 Score: 23.8682 bits (50), Expect = 5.371e-27
Identity = 8/12 (66.67%), Postives = 11/12 (91.67%), Query Frame = 2

Query:    2 KEVGDGNLNFVY 37
            +E+GDGNLN V+
Sbjct:   32 EEIGDGNLNLVF 43

[Close]

BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_YERPP (Methylthioribose kinase OS=Yersinia pestis (strain Pestoides F) GN=mtnK PE=3 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 9.043e-27
Identity = 55/147 (37.41%), Postives = 83/147 (56.46%), Query Frame = 1

Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            +A  L  H Q CP H  +V H D  ++++    L   HI  R  LI G  YP  AE + E++A+TLF+TS  Y++    K  V+ +  N ELC++TE + F+DPY   + N++  P L  +  ++R+D  LKL VA LK  F  +A+
Sbjct:   80 EAETLLTHSQFCPQHTVKVLHHDAELAVMVQEDLSDHHI-WRHELIQGNYYPQAAEQLGEYLAQTLFHTSDFYQSAQAKKAAVSRYT-NPELCQITEDLFFTDPYIEHERNNF-DPVLLPEVLSLRQDKALKLAVASLKHRFLSQAE 223

HSP 2 Score: 44.2838 bits (103), Expect = 9.043e-27
Identity = 16/30 (53.33%), Postives = 24/30 (80.00%), Query Frame = 3

Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSI 155
            ++KQALPYVRC+GESWP+  +RA +   ++
Sbjct:   55 IVKQALPYVRCVGESWPLMLDRARIEAETL 84

HSP 3 Score: 23.483 bits (49), Expect = 9.043e-27
Identity = 8/11 (72.73%), Postives = 10/11 (90.91%), Query Frame = 2

Query:    5 EVGDGNLNFVY 37
            E+GDGNLN V+
Sbjct:   33 EIGDGNLNLVF 43

[Close]

BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_YERPG (Methylthioribose kinase OS=Yersinia pestis bv. Antiqua (strain Angola) GN=mtnK PE=3 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 9.043e-27
Identity = 55/147 (37.41%), Postives = 83/147 (56.46%), Query Frame = 1

Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            +A  L  H Q CP H  +V H D  ++++    L   HI  R  LI G  YP  AE + E++A+TLF+TS  Y++    K  V+ +  N ELC++TE + F+DPY   + N++  P L  +  ++R+D  LKL VA LK  F  +A+
Sbjct:   80 EAETLLTHSQFCPQHTVKVLHHDAELAVMVQEDLSDHHI-WRHELIQGNYYPQAAEQLGEYLAQTLFHTSDFYQSAQAKKAAVSRYT-NPELCQITEDLFFTDPYIEHERNNF-DPVLLPEVLSLRQDKALKLAVASLKHRFLSQAE 223

HSP 2 Score: 44.2838 bits (103), Expect = 9.043e-27
Identity = 16/30 (53.33%), Postives = 24/30 (80.00%), Query Frame = 3

Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSI 155
            ++KQALPYVRC+GESWP+  +RA +   ++
Sbjct:   55 IVKQALPYVRCVGESWPLMLDRARIEAETL 84

HSP 3 Score: 23.483 bits (49), Expect = 9.043e-27
Identity = 8/11 (72.73%), Postives = 10/11 (90.91%), Query Frame = 2

Query:    5 EVGDGNLNFVY 37
            E+GDGNLN V+
Sbjct:   33 EIGDGNLNLVF 43

[Close]

BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_BACCN (Methylthioribose kinase OS=Bacillus cereus subsp. cytotoxis (strain NVH 391-98) GN=mtnK PE=3 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 3.620e-24
Identity = 58/151 (38.41%), Postives = 88/151 (58.28%), Query Frame = 1

Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVRE----DNTLKLEVAGLKSMFCERAQ 582
            ++ AL+   +  P+ VP+VY  D+ +++I M  L    I+ RKG I G +YPLL+EH+  F+ K LFYTS  +   TE KR +     N +LC++TE +VF+DP+     N+ T+ Y +R+  +V E    D  LKL+VA  K  F  R +
Sbjct:   76 ESSALQIFAKYVPEFVPKVYGHDKELAVIVMEDLSSLTIV-RKGFIEGEEYPLLSEHVGRFLGKVLFYTS-DFGLNTEEKRVLDGKFINPDLCKITEDLVFTDPFG----NYETNDY-ERELQSVIEELWCDKNLKLQVAQYKYKFLTRKE 219

HSP 2 Score: 39.2762 bits (90), Expect = 3.620e-24
Identity = 13/31 (41.94%), Postives = 24/31 (77.42%), Query Frame = 3

Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSIE 158
            ++KQALPY + +GESWP++  RA +  ++++
Sbjct:   51 IVKQALPYAKVVGESWPLSLNRATIESSALQ 81

HSP 3 Score: 25.409 bits (54), Expect = 3.620e-24
Identity = 8/12 (66.67%), Postives = 12/12 (100.00%), Query Frame = 2

Query:    2 KEVGDGNLNFVY 37
            +E+GDGNLN+V+
Sbjct:   30 REIGDGNLNYVF 41

[Close]

BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_BACP2 (Methylthioribose kinase OS=Bacillus pumilus (strain SAFR-032) GN=mtnK PE=3 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 1.025e-23
Identity = 53/147 (36.05%), Postives = 85/147 (57.82%), Query Frame = 1

Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            ++ AL +  +  P  VP VY+ D  +++  M  L    I+ RKGLIAG +YP L++H+ EF+ KTLFYTS  + T  + K+   +   N +LC +TE++VF+DP+  S  N +    L   A+A+  D  ++ + A LK +F   A+
Sbjct:   83 ESAALIKQSEYTPHLVPAVYYSDTALAVTAMEDLSHLEIV-RKGLIAGKQYPHLSDHVGEFLGKTLFYTS-DFATNPKIKKQFVKQFTNPDLCDITEKLVFTDPFFDSSTNDFEEE-LREAAEALWADLEVQAKAAELKRIFLTSAE 226

HSP 2 Score: 40.817 bits (94), Expect = 1.025e-23
Identity = 15/30 (50.00%), Postives = 23/30 (76.67%), Query Frame = 3

Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSI 155
            +IKQALPY + +GESWP+T +RA +   ++
Sbjct:   58 IIKQALPYAKVVGESWPLTLDRARIESAAL 87

HSP 3 Score: 24.6386 bits (52), Expect = 1.025e-23
Identity = 8/11 (72.73%), Postives = 11/11 (100.00%), Query Frame = 2

Query:    5 EVGDGNLNFVY 37
            E+GDGNLN+V+
Sbjct:   36 EIGDGNLNYVF 46

[Close]

BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_EXIS2 (Methylthioribose kinase OS=Exiguobacterium sibiricum (strain DSM 17290 / JCM 13490 / 255-15) GN=mtnK PE=3 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 1.026e-23
Identity = 53/140 (37.86%), Postives = 82/140 (58.57%), Query Frame = 1

Query:  151 ALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHI-ILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMF 567
            ALKE  ++    VP VYH  +  +   M  L   H+ I+R GL+ G KYPLLAEH+  ++A+TLFYTS  +      K+ +A    N +LC +TE+++F+DP+  ++ N      L+ + D +  D  LK EVA L+++F
Sbjct:   78 ALKEFAKVAVPFVPHVYHASKEEAFTVMEDLS--HLTIVRGGLLEGEKYPLLAEHIGSYLARTLFYTS-DFAVGPVAKKQIARQYYNPDLCDITEKLIFTDPFYDAETNA-IEAGLEAEVDRIWNDAELKREVAKLEALF 213

HSP 2 Score: 41.2022 bits (95), Expect = 1.026e-23
Identity = 14/31 (45.16%), Postives = 26/31 (83.87%), Query Frame = 3

Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSIE 158
            ++KQALPY + +GESWP++ ERA++  ++++
Sbjct:   50 ILKQALPYAKVVGESWPLSLERAWIEQSALK 80

HSP 3 Score: 23.8682 bits (50), Expect = 1.026e-23
Identity = 8/12 (66.67%), Postives = 11/12 (91.67%), Query Frame = 2

Query:    2 KEVGDGNLNFVY 37
            +E+GDGNLN V+
Sbjct:   29 EEIGDGNLNLVF 40

The following BLAST results are available for this feature:

BLAST of DR908756 vs. ExPASy Swiss-Prot

BLAST of DR908756 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 41


Match Name	E-value	Identity	Description
MTNK_YERPS	2.455e-27	37.41	Methylthioribose kinase OS=Yersinia pseudotubercul...	[more]
MTNK_YERPB	2.455e-27	37.41	Methylthioribose kinase OS=Yersinia pseudotubercul...	[more]
MTNK_YERP3	2.455e-27	37.41	Methylthioribose kinase OS=Yersinia pseudotubercul...	[more]
MTNK_ERWCT	4.138e-27	36.73	Methylthioribose kinase OS=Erwinia carotovora subs...	[more]
MTNK_PECCP	5.371e-27	36.73	Methylthioribose kinase OS=Pectobacterium carotovo...	[more]
MTNK_YERPP	9.043e-27	37.41	Methylthioribose kinase OS=Yersinia pestis (strain...	[more]
MTNK_YERPG	9.043e-27	37.41	Methylthioribose kinase OS=Yersinia pestis bv. Ant...	[more]
MTNK_BACCN	3.620e-24	38.41	Methylthioribose kinase OS=Bacillus cereus subsp. ...	[more]
MTNK_BACP2	1.025e-23	36.05	Methylthioribose kinase OS=Bacillus pumilus (strai...	[more]
MTNK_EXIS2	1.026e-23	37.86	Methylthioribose kinase OS=Exiguobacterium sibiric...	[more]

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Properties


Property Name	Value
Genbank description	USDA-FP_16884 Citrus sinensis phloem Citrus sinensis cDNA clone VPE-41_F04 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>DR908756 ID=DR908756; Name=DR908756; organism=Citrus sinensis; type=EST; length=584bp
CAAAGAAGTCGGAGACGGCAACCTTAATTTCGTCTACATCGTCTGTCGGC
TCTTCTGGTTCTTTCGTCATCAAGCAGGCTCTTCCGTATGTACGTTGTAT
CGGTGAATCATGGCCCATGACAAAGGAAAGAGCGTATTTGCCAGGCACTA
GCATTGAAAGAGCATGGCCAATTGTGTCCTGACCATGTTCCCGAAGTCTA
TCATTTTGACCGTACAATGTCTTTGATTGGTATGCGTTACCTGGAGCCTC
CTCATATTATCCTGAGAAAGGGGTTGATTGCTGGAATAAAGTATCCATTA
CTTGCGGAACACATGTCAGAATTCATGGCAAAGACTCTTTTCTACACATC
TCTTTTATATCGTACCACCACAGAGCACAAACGTAATGTTGCTGAATTTT
GTGGGAATGTGGAGCTATGCAGGCTCACGGAGCAGGTTGTTTTCTCGGAC
CCCTACAAAGTATCTAAGTTTAACCATTGGACTTCTCCTTATCTTGATCG
TGACGCTGACGCTGTTCGGGAGGATAATACATTAAAGCTGGAAGTTGCTG
GGTTGAAATCCATGTTCTGTGAAAGAGCACAAGC