DR908756

Overview
NameDR908756
Unique NameDR908756
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length584
Libraries
Library NameType
Citrus sinensis phloemcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_BACSU (Methylthioribose kinase OS=Bacillus subtilis GN=mtnK PE=1 SV=2)

HSP 1 Score: 81.2629 bits (199), Expect = 1.380e-22
Identity = 54/151 (35.76%), Postives = 87/151 (57.62%), Query Frame = 1 
Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHI-ILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPY---LDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            ++ AL   G+  P  VP V++ D  M++  M  L   H+ I RKGLI G  YP L++H+ EF+ KTLFY+S  Y    + K+ + +   N ELC +TE++VF+DP+    F+H T+ +   L    + +  ++++K+E A LK  F   A+
Sbjct:   84 ESSALIRQGEHVPHLVPRVFYSDTEMAVTVMEDLS--HLKIARKGLIEGENYPHLSQHIGEFLGKTLFYSS-DYALEPKVKKQLVKQFTNPELCDITERLVFTDPF----FDHDTNDFEEELRPFVEKLWNNDSVKIEAAKLKKSFLTSAE 227

HSP 2 Score: 40.4318 bits (93), Expect = 1.380e-22
Identity = 15/32 (46.88%), Postives = 25/32 (78.12%), Query Frame = 3 
Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSIER 161
            +IKQA+PY + +GESWP+T +RA +  +++ R
Sbjct:   59 IIKQAVPYAKVVGESWPLTIDRARIESSALIR 90

HSP 3 Score: 25.0238 bits (53), Expect = 1.380e-22
Identity = 8/12 (66.67%), Postives = 12/12 (100.00%), Query Frame = 2 
Query:    2 KEVGDGNLNFVY 37
            +E+GDGNLN+V+
Sbjct:   36 QEIGDGNLNYVF 47
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_BACA2 (Methylthioribose kinase OS=Bacillus amyloliquefaciens (strain FZB42) GN=mtnK PE=3 SV=1)

HSP 1 Score: 78.1814 bits (191), Expect = 5.062e-22
Identity = 54/151 (35.76%), Postives = 84/151 (55.63%), Query Frame = 1 
Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHI-ILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPY---KVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            ++ AL   G+  P  VP VY+ D  M++  M  L   H+ I RKGL+ G  YP L+ H+ EF+ KTLFY+S  Y    + K+ +A+   N ELC +TE++VF++P+   + + F     PY    A+ V  ++ LK     LK++F   A+
Sbjct:   84 ESSALIRQGEHVPHLVPAVYYSDTEMAVTVMEDLS--HLRISRKGLLEGQHYPNLSAHIGEFLGKTLFYSS-DYALDAKVKQQLAKQFTNPELCNITERLVFTEPFLDHETNDFEEELRPY----AEKVWNNSRLKEGADELKAIFLNSAE 227

HSP 2 Score: 40.817 bits (94), Expect = 5.062e-22
Identity = 15/32 (46.88%), Postives = 25/32 (78.12%), Query Frame = 3 
Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSIER 161
            +IKQA+PY + +GESWP+T +RA +  +++ R
Sbjct:   59 IIKQAIPYAKVVGESWPLTIDRARIESSALIR 90

HSP 3 Score: 25.7942 bits (55), Expect = 5.062e-22
Identity = 9/14 (64.29%), Postives = 13/14 (92.86%), Query Frame = 2 
Query:    2 KEVGDGNLNFVYIV 43
            +E+GDGNLN+V+ V
Sbjct:   36 REIGDGNLNYVFHV 49
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_GEOSW (Methylthioribose kinase OS=Geobacillus sp. (strain WCH70) GN=mtnK PE=3 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 5.415e-22
Identity = 60/151 (39.74%), Postives = 91/151 (60.26%), Query Frame = 1 
Query:  127 KERICQALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHI-ILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDR---DADAVREDNTLKLEVAGLKSMF 567
            K  + ++ AL+      P +VP+VY+ D ++++  M  L   H+ I RKGLI G  YPLL++H+ EF+AKT FYTS  +    + K+ +A+   N ELC++TE +VF+DP+    FNH T+ + D    D +A+  D+ L LE A LK  F
Sbjct:   79 KRAVIESNALRTFASYVPQYVPKVYYSDESLAITVMEDLS--HLQIARKGLIEGKTYPLLSQHIGEFIAKTSFYTS-DFGMNQQEKKQLAQSFVNPELCKITEDLVFTDPF----FNHETNNFEDELRDDVEALWNDDKLHLEAAKLKRKF 222

HSP 2 Score: 24.6386 bits (52), Expect = 5.415e-22
Identity = 9/14 (64.29%), Postives = 12/14 (85.71%), Query Frame = 2 
Query:    2 KEVGDGNLNFVYIV 43
            +E+GDGNLN V+ V
Sbjct:   36 REIGDGNLNLVFRV 49
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_GEOKA (Methylthioribose kinase OS=Geobacillus kaustophilus GN=mtnK PE=3 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 2.018e-21
Identity = 59/154 (38.31%), Postives = 90/154 (58.44%), Query Frame = 1 
Query:  127 KERICQALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDR---DADAVREDNTLKLEVAGLKSMFCERA 579
            K  + ++ AL+      P +VP+VY+ D ++++  M  L    I  RKGLI G  YPLL++H+ EF+AKT FYTS  +    + K+ +A+   N ELC++TE +VF+DP+    F+H T+ + D    D +A+  D+ L LE A LK  F   A
Sbjct:   79 KRAVIESNALRTFASYVPQYVPKVYYSDESLAITVMEDLSRLQIA-RKGLIEGKTYPLLSQHIGEFVAKTAFYTS-DFGMNQQEKKKLAQSFVNPELCKITEDLVFTDPF----FDHETNNFEDELRPDVEALWRDDRLHLEAAKLKRKFLTEA 226

HSP 2 Score: 24.2534 bits (51), Expect = 2.018e-21
Identity = 8/12 (66.67%), Postives = 11/12 (91.67%), Query Frame = 2 
Query:    2 KEVGDGNLNFVY 37
            +E+GDGNLN V+
Sbjct:   36 REIGDGNLNLVF 47
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_BACLD (Methylthioribose kinase OS=Bacillus licheniformis (strain DSM 13 / ATCC 14580) GN=mtnK PE=3 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.141e-20
Identity = 52/146 (35.62%), Postives = 81/146 (55.48%), Query Frame = 1 
Query:  142 QALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHI-ILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPY---LDRDADAVREDNTLKLEVAGLKSMF 567
            ++ AL    +  P  VP+VY+ D  +++  +  L   H+ I R GLI G  YP L+E + EF+ KT FY+S      T  +R V +F  N +LC +TE +VF+DP+    F+H T+ +   L  +A+ +  +  LK+E A LK  F
Sbjct:   84 ESSALIRQAEHVPHLVPKVYYSDTELAVTVLEDLS--HLEIARNGLIDGKDYPHLSEDIGEFLGKTHFYSSEYALDPTVKERLVKQFT-NPDLCDITESLVFTDPF----FDHETNDFEEELRDEAEVLWGNEALKIEAAKLKQRF 222

HSP 2 Score: 41.2022 bits (95), Expect = 1.141e-20
Identity = 16/32 (50.00%), Postives = 25/32 (78.12%), Query Frame = 3 
Query:   66 VIKQALPYVRCIGESWPMTKERAYLPGTSIER 161
            VIKQA+PY + +GESWP+T +RA +  +++ R
Sbjct:   59 VIKQAVPYAKVVGESWPLTLDRARIESSALIR 90

HSP 3 Score: 24.6386 bits (52), Expect = 1.141e-20
Identity = 9/14 (64.29%), Postives = 12/14 (85.71%), Query Frame = 2 
Query:    2 KEVGDGNLNFVYIV 43
            +E+GDGNLN V+ V
Sbjct:   36 REIGDGNLNLVFRV 49
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_GEOTN (Methylthioribose kinase OS=Geobacillus thermodenitrificans (strain NG80-2) GN=mtnK PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 2.799e-20
Identity = 58/157 (36.94%), Postives = 90/157 (57.32%), Query Frame = 1 
Query:  127 KERICQALALKEHGQLCPDHVPEVYHFDRTMSLIGMR---YLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDR---DADAVREDNTLKLEVAGLKSMFCERA 579
            K  + ++ AL+      P +VP+VY+ D ++++  M    YL+    I RKGLI G  YPLL+ H+ EF+AKT FYTS  +    + K+ +A+   N ELC++TE +VF+DP+    F+H ++ + D    D + +  D+ L LE A LK  F   A
Sbjct:   79 KRAVIESNALRTFASYVPQYVPKVYYSDESLAITVMEDLSYLQ----IARKGLIEGKTYPLLSRHIGEFIAKTAFYTS-DFGMNQQEKKKLAQSFVNPELCKITEDLVFTDPF----FDHDSNNFEDELHLDVETLWNDDRLHLEAAKLKRKFLTEA 226

HSP 2 Score: 24.6386 bits (52), Expect = 2.799e-20
Identity = 9/14 (64.29%), Postives = 12/14 (85.71%), Query Frame = 2 
Query:    2 KEVGDGNLNFVYIV 43
            +E+GDGNLN V+ V
Sbjct:   36 REIGDGNLNLVFHV 49
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_BACC2 (Methylthioribose kinase OS=Bacillus cereus (strain G9842) GN=mtnK PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 1.442e-18
Identity = 57/152 (37.50%), Postives = 83/152 (54.61%), Query Frame = 1 
Query:  127 KERICQALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            K    ++ AL+   Q  PD+VP VY  D  +++  +  L     I RKGLI G +YPLL++H+  F+A  LFYTS  +   +E KR +     N +LC++TE +VF+DP+     N +  P L    D +  D TLKL+VA  K  F  R +
Sbjct:   71 KRATIESKALQIFAQYVPDYVPAVYSHDEELAITVIEDLSRL-TITRKGLIGGEEYPLLSQHIGRFLAHVLFYTS-DFGLQSEEKRVLEGTFVNPDLCKITEDLVFTDPFGHYDTNDY-EPDLQLVVDELWSDKTLKLKVAQYKYKFLTRKE 219

HSP 2 Score: 24.6386 bits (52), Expect = 1.442e-18
Identity = 8/11 (72.73%), Postives = 11/11 (100.00%), Query Frame = 2 
Query:    5 EVGDGNLNFVY 37
            E+GDGNLN+V+
Sbjct:   31 EIGDGNLNYVF 41
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_BACCR (Methylthioribose kinase OS=Bacillus cereus (strain ATCC 14579 / DSM 31) GN=mtnK PE=3 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 3.170e-18
Identity = 57/152 (37.50%), Postives = 83/152 (54.61%), Query Frame = 1 
Query:  127 KERICQALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            K    ++ AL+   Q  PD+VP VY  D  +++  +  L     I RKGLI G +YPLL++H+  F+A  LFYTS  +   +E KR +     N +LC++TE +VF+DP+     N +  P L    D +  D TLKL+VA  K  F  R +
Sbjct:   71 KRATIESKALQIFAQYVPDYVPVVYSHDEELAITVIEDLSRL-TITRKGLIDGEEYPLLSQHIGRFLAHVLFYTS-DFGLQSEEKRILEGTFVNPDLCKITEDLVFTDPFGHYDTNDY-EPDLQLVVDELWSDKTLKLKVAQYKYKFLTRKE 219

HSP 2 Score: 24.6386 bits (52), Expect = 3.170e-18
Identity = 8/11 (72.73%), Postives = 11/11 (100.00%), Query Frame = 2 
Query:    5 EVGDGNLNFVY 37
            E+GDGNLN+V+
Sbjct:   31 EIGDGNLNYVF 41
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_BACC4 (Methylthioribose kinase OS=Bacillus cereus (strain B4264) GN=mtnK PE=3 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 3.170e-18
Identity = 57/152 (37.50%), Postives = 83/152 (54.61%), Query Frame = 1 
Query:  127 KERICQALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            K    ++ AL+   Q  PD+VP VY  D  +++  +  L     I RKGLI G +YPLL++H+  F+A  LFYTS  +   +E KR +     N +LC++TE +VF+DP+     N +  P L    D +  D TLKL+VA  K  F  R +
Sbjct:   71 KRATIESKALQIFAQYVPDYVPVVYSHDEELAITVIEDLSRL-TITRKGLIDGEEYPLLSQHIGRFLAHVLFYTS-DFGLQSEEKRILEGTFVNPDLCKITEDLVFTDPFGHYDTNDY-EPDLQLVVDELWSDKTLKLKVAQYKYKFLTRKE 219

HSP 2 Score: 24.6386 bits (52), Expect = 3.170e-18
Identity = 8/11 (72.73%), Postives = 11/11 (100.00%), Query Frame = 2 
Query:    5 EVGDGNLNFVY 37
            E+GDGNLN+V+
Sbjct:   31 EIGDGNLNYVF 41
[Close]
BLAST of DR908756 vs. ExPASy Swiss-Prot
Match: MTNK_BACC1 (Methylthioribose kinase OS=Bacillus cereus (strain ATCC 10987) GN=mtnK PE=3 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 3.170e-18
Identity = 56/152 (36.84%), Postives = 83/152 (54.61%), Query Frame = 1 
Query:  127 KERICQALALKEHGQLCPDHVPEVYHFDRTMSLIGMRYLEPPHIILRKGLIAGIKYPLLAEHMSEFMAKTLFYTSLLYRTTTEHKRNVAEFCGNVELCRLTEQVVFSDPYKVSKFNHWTSPYLDRDADAVREDNTLKLEVAGLKSMFCERAQ 582
            K    ++ ALK   +  PD+VP V+  D  +++  +  L     I RKGLI G +YPLL++H+  F+A  LFYTS  +   +E KR +     N +LC++TE +VF+DP+     N +  P L    D +  D TLKL+VA  K  F  R +
Sbjct:   71 KRATIESKALKIFAKYVPDYVPVVHSHDEELAVTVIEDLSRL-TITRKGLIDGEEYPLLSQHIGRFLANVLFYTS-DFGLQSEEKRGLEGTFVNPDLCKITEDLVFTDPFGHYDTNDY-EPELQLAVDELWSDKTLKLKVAQYKYKFLTRKE 219

HSP 2 Score: 24.6386 bits (52), Expect = 3.170e-18
Identity = 8/11 (72.73%), Postives = 11/11 (100.00%), Query Frame = 2 
Query:    5 EVGDGNLNFVY 37
            E+GDGNLN+V+
Sbjct:   31 EIGDGNLNYVF 41
The following BLAST results are available for this feature:
BLAST of DR908756 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 41
Match NameE-valueIdentityDescription
MTNK_BACSU1.380e-2235.76Methylthioribose kinase OS=Bacillus subtilis GN=mt... [more]
MTNK_BACA25.062e-2235.76Methylthioribose kinase OS=Bacillus amyloliquefaci... [more]
MTNK_GEOSW5.415e-2239.74Methylthioribose kinase OS=Geobacillus sp. (strain... [more]
MTNK_GEOKA2.018e-2138.31Methylthioribose kinase OS=Geobacillus kaustophilu... [more]
MTNK_BACLD1.141e-2035.62Methylthioribose kinase OS=Bacillus licheniformis ... [more]
MTNK_GEOTN2.799e-2036.94Methylthioribose kinase OS=Geobacillus thermodenit... [more]
MTNK_BACC21.442e-1837.50Methylthioribose kinase OS=Bacillus cereus (strain... [more]
MTNK_BACCR3.170e-1837.50Methylthioribose kinase OS=Bacillus cereus (strain... [more]
MTNK_BACC43.170e-1837.50Methylthioribose kinase OS=Bacillus cereus (strain... [more]
MTNK_BACC13.170e-1836.84Methylthioribose kinase OS=Bacillus cereus (strain... [more]

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Properties
Property NameValue
Genbank descriptionUSDA-FP_16884 Citrus sinensis phloem Citrus sinensis cDNA clone VPE-41_F04 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DR908756 ID=DR908756; Name=DR908756; organism=Citrus sinensis; type=EST; length=584bp
CAAAGAAGTCGGAGACGGCAACCTTAATTTCGTCTACATCGTCTGTCGGC
TCTTCTGGTTCTTTCGTCATCAAGCAGGCTCTTCCGTATGTACGTTGTAT
CGGTGAATCATGGCCCATGACAAAGGAAAGAGCGTATTTGCCAGGCACTA
GCATTGAAAGAGCATGGCCAATTGTGTCCTGACCATGTTCCCGAAGTCTA
TCATTTTGACCGTACAATGTCTTTGATTGGTATGCGTTACCTGGAGCCTC
CTCATATTATCCTGAGAAAGGGGTTGATTGCTGGAATAAAGTATCCATTA
CTTGCGGAACACATGTCAGAATTCATGGCAAAGACTCTTTTCTACACATC
TCTTTTATATCGTACCACCACAGAGCACAAACGTAATGTTGCTGAATTTT
GTGGGAATGTGGAGCTATGCAGGCTCACGGAGCAGGTTGTTTTCTCGGAC
CCCTACAAAGTATCTAAGTTTAACCATTGGACTTCTCCTTATCTTGATCG
TGACGCTGACGCTGTTCGGGAGGATAATACATTAAAGCTGGAAGTTGCTG
GGTTGAAATCCATGTTCTGTGAAAGAGCACAAGC
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