CN189499

Overview
NameCN189499
Unique NameCN189499
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length764
Libraries
Library NameType
Washington Navel Orange Stored Fruit Rind cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN189499 vs. ExPASy Swiss-Prot
Match: ISPG_PSEA7 (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Pseudomonas aeruginosa (strain PA7) GN=ispG PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 8.009e-21
Identity = 57/117 (48.72%), Postives = 76/117 (64.96%), Query Frame = 1 
Query:  313 SIHKT---VRRKTRTVMVGNVAIGSEHPIRVQTMTTNDTKDVAGTVEEVMRIADQGADLVRITVQGKREADACFEIKNTLVQKNYNIPLVADIHFAPSVALRVAEC-FDKIRVNPGN 651
            SIH      RRK+R + VGNV +G + PI VQ+MT  +T DVA TV ++ R+ D GAD+VR++V     A+A  +IK     +  N+PLVADIHF   +ALRVAE   D +R+NPGN
Sbjct:    2 SIHSASPITRRKSRKIWVGNVPVGGDAPIAVQSMTNTETCDVAATVAQIRRLEDAGADIVRVSVPDMDAAEAFGKIK-----QQVNVPLVADIHFDYRIALRVAELGVDCLRINPGN 113
[Close]
BLAST of CN189499 vs. ExPASy Swiss-Prot
Match: ISPG_PSEE4 (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Pseudomonas entomophila (strain L48) GN=ispG PE=3 SV=1)

HSP 1 Score: 99.3673 bits (246), Expect = 2.330e-20
Identity = 53/108 (49.07%), Postives = 72/108 (66.67%), Query Frame = 1 
Query:  331 RRKTRTVMVGNVAIGSEHPIRVQTMTTNDTKDVAGTVEEVMRIADQGADLVRITVQGKREADACFEIKNTLVQKNYNIPLVADIHFAPSVALRVAEC-FDKIRVNPGN 651
            RR++R + VGNV +G + PI VQ+MT  DT DVA TV ++ R+ D G D+VR++V     A+A  +IK     K  ++PLVADIHF   +ALRVAE   D +R+NPGN
Sbjct:    9 RRESRKIWVGNVPVGGDAPIAVQSMTNTDTNDVAATVAQIQRLVDAGVDIVRVSVPDMDAAEAFGKIK-----KLVSVPLVADIHFDYKIALRVAELGVDCLRINPGN 111
[Close]
BLAST of CN189499 vs. ExPASy Swiss-Prot
Match: ISPG_PSEPK (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Pseudomonas putida (strain KT2440) GN=ispG PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.043e-20
Identity = 52/108 (48.15%), Postives = 72/108 (66.67%), Query Frame = 1 
Query:  331 RRKTRTVMVGNVAIGSEHPIRVQTMTTNDTKDVAGTVEEVMRIADQGADLVRITVQGKREADACFEIKNTLVQKNYNIPLVADIHFAPSVALRVAEC-FDKIRVNPGN 651
            RR++R + VGNV +G + PI VQ+MT  DT DVA TV ++ R+ D G D+VR++V     A+A  +IK     +  ++PLVADIHF   +ALRVAE   D +R+NPGN
Sbjct:    9 RRESRKIWVGNVPVGGDAPIAVQSMTNTDTNDVAATVAQIQRLVDAGVDIVRVSVPDMDAAEAFGKIK-----QRVSVPLVADIHFDYKIALRVAELGVDCLRINPGN 111
[Close]
BLAST of CN189499 vs. ExPASy Swiss-Prot
Match: ISPG_PSEPG (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Pseudomonas putida (strain GB-1) GN=ispG PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.043e-20
Identity = 52/108 (48.15%), Postives = 72/108 (66.67%), Query Frame = 1 
Query:  331 RRKTRTVMVGNVAIGSEHPIRVQTMTTNDTKDVAGTVEEVMRIADQGADLVRITVQGKREADACFEIKNTLVQKNYNIPLVADIHFAPSVALRVAEC-FDKIRVNPGN 651
            RR++R + VGNV +G + PI VQ+MT  DT DVA TV ++ R+ D G D+VR++V     A+A  +IK  +     ++PLVADIHF   +ALRVAE   D +R+NPGN
Sbjct:    9 RRESRKIWVGNVPVGGDAPIAVQSMTNTDTNDVAATVAQIQRLVDAGVDIVRVSVPDMDAAEAFGKIKQLV-----SVPLVADIHFDYKIALRVAELGVDCLRINPGN 111
[Close]
BLAST of CN189499 vs. ExPASy Swiss-Prot
Match: ISPG_PSEP1 (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=ispG PE=3 SV=1)

HSP 1 Score: 98.9821 bits (245), Expect = 3.043e-20
Identity = 52/108 (48.15%), Postives = 72/108 (66.67%), Query Frame = 1 
Query:  331 RRKTRTVMVGNVAIGSEHPIRVQTMTTNDTKDVAGTVEEVMRIADQGADLVRITVQGKREADACFEIKNTLVQKNYNIPLVADIHFAPSVALRVAEC-FDKIRVNPGN 651
            RR++R + VGNV +G + PI VQ+MT  DT DVA TV ++ R+ D G D+VR++V     A+A  +IK     +  ++PLVADIHF   +ALRVAE   D +R+NPGN
Sbjct:    9 RRESRKIWVGNVPVGGDAPIAVQSMTNTDTNDVAATVAQIQRLVDAGVDIVRVSVPDMDAAEAFGKIK-----QRVSVPLVADIHFDYKIALRVAELGVDCLRINPGN 111
[Close]
BLAST of CN189499 vs. ExPASy Swiss-Prot
Match: ISPG_PSEPW (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Pseudomonas putida (strain W619) GN=ispG PE=3 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 3.975e-20
Identity = 52/108 (48.15%), Postives = 72/108 (66.67%), Query Frame = 1 
Query:  331 RRKTRTVMVGNVAIGSEHPIRVQTMTTNDTKDVAGTVEEVMRIADQGADLVRITVQGKREADACFEIKNTLVQKNYNIPLVADIHFAPSVALRVAEC-FDKIRVNPGN 651
            RR++R + VGNV +G + PI VQ+MT  DT DVA TV ++ R+ D G D+VR++V     A+A  +IK  +     ++PLVADIHF   +ALRVAE   D +R+NPGN
Sbjct:    9 RRESRKIWVGNVPVGGDAPIAVQSMTNTDTNDVAATVGQIQRLVDAGVDIVRVSVPDMDAAEAFGKIKQLV-----SVPLVADIHFDYKIALRVAELGVDCLRINPGN 111
[Close]
BLAST of CN189499 vs. ExPASy Swiss-Prot
Match: ISPG_PSEMY (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Pseudomonas mendocina (strain ymp) GN=ispG PE=3 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 5.191e-20
Identity = 56/117 (47.86%), Postives = 75/117 (64.10%), Query Frame = 1 
Query:  304 YCESIHKTVRRKTRTVMVGNVAIGSEHPIRVQTMTTNDTKDVAGTVEEVMRIADQGADLVRITVQGKREADACFEIKNTLVQKNYNIPLVADIHFAPSVALRVAEC-FDKIRVNPGN 651
            +CES  K  RR +R + VG+V +G + PI VQ+MT  DT DVA TV ++ R+ D GAD+VR++V     A+A   IK     +   +PLVADIHF   +ALRVAE   D +R+NPGN
Sbjct:    2 HCESPIK--RRLSRKIWVGSVPVGGDAPIAVQSMTNTDTNDVAATVAQIRRLEDAGADIVRVSVPDMDAAEAFGRIK-----QQVRVPLVADIHFDYQIALRVAELGVDCLRINPGN 111
[Close]
BLAST of CN189499 vs. ExPASy Swiss-Prot
Match: ISPG_BACCR (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Bacillus cereus (strain ATCC 14579 / DSM 31) GN=ispG PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.156e-19
Identity = 54/114 (47.37%), Postives = 70/114 (61.40%), Query Frame = 1 
Query:  325 TVRRKTRTVMVGNVAIGSEHPIRVQTMTTNDTKDVAGTVEEVMRIADQGADLVRITVQGKREADACFEIKNTLVQKNYNIPLVADIHFAPSVALRVAE-CFDKIRVNPGNFADR 663
            T R KTR V VGN+ IG  + + +Q+MTT  T DV  TV E+ R+ + G  +VR+ V  +R ADA  +IK     K  NIPLVADIHF   +AL+  E   DK+R+NPGN   R
Sbjct:    5 THRTKTRPVKVGNLTIGGNNELIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVAVPDERAADAIADIK-----KQINIPLVADIHFDYRLALKAIEGGIDKVRINPGNIGRR 113
[Close]
BLAST of CN189499 vs. ExPASy Swiss-Prot
Match: ISPG_BACC4 (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Bacillus cereus (strain B4264) GN=ispG PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.156e-19
Identity = 54/114 (47.37%), Postives = 70/114 (61.40%), Query Frame = 1 
Query:  325 TVRRKTRTVMVGNVAIGSEHPIRVQTMTTNDTKDVAGTVEEVMRIADQGADLVRITVQGKREADACFEIKNTLVQKNYNIPLVADIHFAPSVALRVAE-CFDKIRVNPGNFADR 663
            T R KTR V VGN+ IG  + + +Q+MTT  T DV  TV E+ R+ + G  +VR+ V  +R ADA  +IK     K  NIPLVADIHF   +AL+  E   DK+R+NPGN   R
Sbjct:    5 THRTKTRPVKVGNLTIGGNNELIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVAVPDERAADAIADIK-----KQINIPLVADIHFDYRLALKAIEGGIDKVRINPGNIGRR 113
[Close]
BLAST of CN189499 vs. ExPASy Swiss-Prot
Match: ISPG_BACC2 (4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase OS=Bacillus cereus (strain G9842) GN=ispG PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 1.156e-19
Identity = 54/114 (47.37%), Postives = 70/114 (61.40%), Query Frame = 1 
Query:  325 TVRRKTRTVMVGNVAIGSEHPIRVQTMTTNDTKDVAGTVEEVMRIADQGADLVRITVQGKREADACFEIKNTLVQKNYNIPLVADIHFAPSVALRVAE-CFDKIRVNPGNFADR 663
            T R KTR V VGN+ IG  + + +Q+MTT  T DV  TV E+ R+ + G  +VR+ V  +R ADA  +IK     K  NIPLVADIHF   +AL+  E   DK+R+NPGN   R
Sbjct:    5 THRTKTRPVKVGNLTIGGNNELIIQSMTTTKTHDVEATVAEIKRLEEAGCQVVRVAVPDERAADAIADIK-----KQINIPLVADIHFDYRLALKAIEGGIDKVRINPGNIGRR 113
The following BLAST results are available for this feature:
BLAST of CN189499 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 424
Match NameE-valueIdentityDescription
ISPG_PSEA78.009e-2148.724-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha... [more]
ISPG_PSEE42.330e-2049.074-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha... [more]
ISPG_PSEPK3.043e-2048.154-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha... [more]
ISPG_PSEPG3.043e-2048.154-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha... [more]
ISPG_PSEP13.043e-2048.154-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha... [more]
ISPG_PSEPW3.975e-2048.154-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha... [more]
ISPG_PSEMY5.191e-2047.864-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha... [more]
ISPG_BACCR1.156e-1947.374-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha... [more]
ISPG_BACC41.156e-1947.374-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha... [more]
ISPG_BACC21.156e-1947.374-hydroxy-3-methylbut-2-en-1-yl diphosphate syntha... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS06_0001P11_r Washington Navel Orange Stored Fruit Rind cDNA Library Citrus sinensis cDNA clone CS_WEc0001P11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN189499 ID=CN189499; Name=CN189499; organism=Citrus sinensis; type=EST; length=764bp
CGTTCAGACTCATCACATTTCTCTCTCAGAGTTAGTTCTCACTTCTCAGC
ATTATAAAAGGCGTAGTATTTTGCAAAGATGGCGACTGGAACCCTGCCCG
CTTCGTTTCCGGGCCTAAAGAGCAGAGACTCGGGCTTGGGGTTTGCGAAA
AGTGTGGATTTTGTGAGAGTTTGTGATTTCAGAAAGTTCAAGTCTGGCAG
AAGAAGATTCACTGTGATAAGGAATTCATCAAATTCCAGTTCGGATATCG
CTGAGCTTCAGCCTGCATCAGAAGGAAGCCCCCTATTAGTTCCAAGACAA
AAGTATTGTGAATCCATTCACAAAACTGTCAGAAGGAAGACACGGACGGT
GATGGTGGGAAATGTAGCCATTGGCAGTGAGCATCCTATTAGAGTTCAAA
CAATGACTACAAATGACACTAAGGATGTTGCTGGAACAGTGGAAGAGGTA
ATGAGAATAGCAGACCAGGGAGCAGATCTTGTTCGGATCACAGTTCAAGG
GAAGAGAGAGGCAGATGCATGTTTTGAGATAAAAAACACACTTGTCCAGA
AAAACTACAATATACCTCTGGTGGCAGATATTCATTTTGCCCCTTCTGTT
GCTCTACGAGTTGCTGAATGCTTTGACAAAATCCGTGTCAACCCAGGAAA
TTTTGCTGATAGACGGGCTCAGTTTCAGCAGCTAGAGTACACAGACGATG
AGTATCAGAAAGAACTTGAACATATTGAGGAGGTTTTTAGTCCATTGGTT
GAGAAATGTAAAAA
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