CN189435

Overview
NameCN189435
Unique NameCN189435
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length709
Libraries
Library NameType
Washington Navel Orange Stored Fruit Rind cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN189435 vs. ExPASy Swiss-Prot
Match: IF4A_NEUCR (ATP-dependent RNA helicase eIF4A OS=Neurospora crassa GN=tif-1 PE=3 SV=2)

HSP 1 Score: 219.55 bits (558), Expect = 1.352e-56
Identity = 107/161 (66.46%), Postives = 131/161 (81.37%), Query Frame = 3 
Query:  225 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADE 707
            ++YDE  DSFD M L+  LLRGIYAYGFE+PSAIQQR I+P  KG DVI QAQSGTGKTATF   +LQ++D  L  CQAL+LAPTRELAQQI+KV+ A+GD++ ++ HAC+GGTSVR+D + LQ G  VVVGTPGRV DM++R+ L+ D +KMFVLDEADE
Sbjct:   17 SNYDETVDSFDEMNLKPELLRGIYAYGFERPSAIQQRAIMPVIKGHDVIAQAQSGTGKTATFSISVLQKIDPSLKACQALILAPTRELAQQIQKVVVAIGDFMNIECHACIGGTSVRDDMKALQDGPQVVVGTPGRVHDMIQRRFLKTDSMKMFVLDEADE 177
[Close]
BLAST of CN189435 vs. ExPASy Swiss-Prot
Match: IF4A2_RAT (Eukaryotic initiation factor 4A-II OS=Rattus norvegicus GN=Eif4a2 PE=1 SV=1)

HSP 1 Score: 219.55 bits (558), Expect = 1.352e-56
Identity = 111/162 (68.52%), Postives = 132/162 (81.48%), Query Frame = 3 
Query:  225 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQA-GVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADE 707
            ++++E+ D+FD M L+E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQL+    + QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR + + LQA   H+VVGTPGRVFDML R+ L P +IKMFVLDEADE
Sbjct:   26 SNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADE 187
[Close]
BLAST of CN189435 vs. ExPASy Swiss-Prot
Match: IF4A2_PONAB (Eukaryotic initiation factor 4A-II OS=Pongo abelii GN=EIF4A2 PE=2 SV=1)

HSP 1 Score: 219.55 bits (558), Expect = 1.352e-56
Identity = 111/162 (68.52%), Postives = 132/162 (81.48%), Query Frame = 3 
Query:  225 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQA-GVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADE 707
            ++++E+ D+FD M L+E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQL+    + QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR + + LQA   H+VVGTPGRVFDML R+ L P +IKMFVLDEADE
Sbjct:   26 SNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADE 187
[Close]
BLAST of CN189435 vs. ExPASy Swiss-Prot
Match: IF4A2_MOUSE (Eukaryotic initiation factor 4A-II OS=Mus musculus GN=Eif4a2 PE=2 SV=2)

HSP 1 Score: 219.55 bits (558), Expect = 1.352e-56
Identity = 111/162 (68.52%), Postives = 132/162 (81.48%), Query Frame = 3 
Query:  225 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQA-GVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADE 707
            ++++E+ D+FD M L+E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQL+    + QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR + + LQA   H+VVGTPGRVFDML R+ L P +IKMFVLDEADE
Sbjct:   26 SNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADE 187
[Close]
BLAST of CN189435 vs. ExPASy Swiss-Prot
Match: IF4A2_HUMAN (Eukaryotic initiation factor 4A-II OS=Homo sapiens GN=EIF4A2 PE=1 SV=2)

HSP 1 Score: 219.55 bits (558), Expect = 1.352e-56
Identity = 111/162 (68.52%), Postives = 132/162 (81.48%), Query Frame = 3 
Query:  225 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQA-GVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADE 707
            ++++E+ D+FD M L+E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQL+    + QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR + + LQA   H+VVGTPGRVFDML R+ L P +IKMFVLDEADE
Sbjct:   26 SNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADE 187
[Close]
BLAST of CN189435 vs. ExPASy Swiss-Prot
Match: IF4A2_BOVIN (Eukaryotic initiation factor 4A-II OS=Bos taurus GN=EIF4A2 PE=2 SV=1)

HSP 1 Score: 219.55 bits (558), Expect = 1.352e-56
Identity = 111/162 (68.52%), Postives = 132/162 (81.48%), Query Frame = 3 
Query:  225 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQA-GVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADE 707
            ++++E+ D+FD M L+E+LLRGIYAYGFEKPSAIQQR I+P  KG DVI QAQSGTGKTATF   ILQQL+    + QALVLAPTRELAQQI+KV+ ALGDY+G   HAC+GGT+VR + + LQA   H+VVGTPGRVFDML R+ L P +IKMFVLDEADE
Sbjct:   26 SNWNEIVDNFDDMNLKESLLRGIYAYGFEKPSAIQQRAIIPCIKGYDVIAQAQSGTGKTATFAISILQQLEIEFKETQALVLAPTRELAQQIQKVILALGDYMGATCHACIGGTNVRNEMQKLQAEAPHIVVGTPGRVFDMLNRRYLSPKWIKMFVLDEADE 187
[Close]
BLAST of CN189435 vs. ExPASy Swiss-Prot
Match: IF4A_PHANO (ATP-dependent RNA helicase eIF4A OS=Phaeosphaeria nodorum GN=TIF1 PE=3 SV=1)

HSP 1 Score: 219.164 bits (557), Expect = 1.766e-56
Identity = 107/161 (66.46%), Postives = 131/161 (81.37%), Query Frame = 3 
Query:  225 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADE 707
            ++YDE  DSFDAM L+  LLRG+YAYGFE+PSAIQQR I+P  KG DVI QAQSGTGKTATF    LQ++D  +  CQAL+LAPTRELAQQI+KV+ A+GD++ V  HAC+GGTSVR+D + LQ G  VVVGTPGRV DM++R+ L+ D++KMFVLDEADE
Sbjct:   16 SNYDETTDSFDAMNLKAELLRGVYAYGFERPSAIQQRAIMPVIKGHDVIAQAQSGTGKTATFSISTLQKIDSNVKACQALILAPTRELAQQIQKVVVAIGDFMDVACHACIGGTSVRDDMKALQDGPQVVVGTPGRVHDMIQRRVLKTDHMKMFVLDEADE 176
[Close]
BLAST of CN189435 vs. ExPASy Swiss-Prot
Match: IF4A_NEOFI (ATP-dependent RNA helicase eIF4A OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / FGSC A1164 / NRRL 181) GN=tif1 PE=3 SV=1)

HSP 1 Score: 219.164 bits (557), Expect = 1.766e-56
Identity = 107/161 (66.46%), Postives = 132/161 (81.99%), Query Frame = 3 
Query:  225 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADE 707
            ++YDE+ DSFD+M L+  LLRGIYAYGFE+PSAIQQR I+P  KG DVI QAQSGTGKTATF    LQ+LD  +  CQAL+LAPTRELAQQI+KV+ A+GD++ ++ HAC+GGT+VRED + LQ G  VVVGTPGRV DM++R+ LR D +KMF+LDEADE
Sbjct:   18 SNYDEITDSFDSMELKPELLRGIYAYGFERPSAIQQRAILPIIKGNDVIAQAQSGTGKTATFSISALQKLDPNVKGCQALILAPTRELAQQIQKVVVAIGDFMNIECHACIGGTNVREDMKALQEGPQVVVGTPGRVQDMIQRRVLRTDNLKMFILDEADE 178
[Close]
BLAST of CN189435 vs. ExPASy Swiss-Prot
Match: IF4A_COCIM (ATP-dependent RNA helicase eIF4A OS=Coccidioides immitis GN=TIF1 PE=3 SV=1)

HSP 1 Score: 218.394 bits (555), Expect = 3.012e-56
Identity = 107/161 (66.46%), Postives = 131/161 (81.37%), Query Frame = 3 
Query:  225 TSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADE 707
            ++YDE+ DSFDAM L+  LLRG+YAYGFE+PSAIQQR I+P  KG DVI QAQSGTGKTATF    LQ++D  L  CQAL+LAPTRELAQQI+KV+ A+GD++ V+ HAC+GGT+VRED + L  G  VVVGTPGRV DM++R+ L+ D +KMFVLDEADE
Sbjct:   18 SNYDEITDSFDAMNLRAELLRGVYAYGFERPSAIQQRAIMPVIKGSDVIAQAQSGTGKTATFSISALQKVDTNLKACQALILAPTRELAQQIQKVVVAIGDFMSVECHACIGGTNVREDIKALNDGPQVVVGTPGRVHDMIQRRVLKTDQMKMFVLDEADE 178
[Close]
BLAST of CN189435 vs. ExPASy Swiss-Prot
Match: IF4A_ASPOR (ATP-dependent RNA helicase eIF4A OS=Aspergillus oryzae GN=tif1 PE=3 SV=1)

HSP 1 Score: 218.394 bits (555), Expect = 3.012e-56
Identity = 108/176 (61.36%), Postives = 136/176 (77.27%), Query Frame = 3 
Query:  180 TKMNELLSTDGQDFFTSYDEVYDSFDAMGLQENLLRGIYAYGFEKPSAIQQRGIVPFCKGLDVIQQAQSGTGKTATFCSGILQQLDYGLVQCQALVLAPTRELAQQIEKVMRALGDYLGVKVHACVGGTSVREDQRILQAGVHVVVGTPGRVFDMLRRQSLRPDYIKMFVLDEADE 707
            +++  L  + GQ   T+YDE+ DSFDAM L+  LLRG+YAYGFE+PSAIQQR I P  KG DVI QAQSGTGKTATF    LQ+LD  +  CQAL+LAPTRELAQQI+KV+ A+GD++ +  HAC+GGT+VRED + L  G  VVVGTPGRV DM++R+ L+ D++K F+LDEADE
Sbjct:   27 SRLTRLFQSTGQ-IETNYDEITDSFDAMELKPELLRGVYAYGFERPSAIQQRAIKPIIKGSDVIAQAQSGTGKTATFSISALQKLDPNVKGCQALILAPTRELAQQIQKVVVAIGDFMNITCHACIGGTAVREDMKALGEGPEVVVGTPGRVHDMIQRRVLKTDHLKQFILDEADE 201
The following BLAST results are available for this feature:
BLAST of CN189435 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
IF4A_NEUCR1.352e-5666.46ATP-dependent RNA helicase eIF4A OS=Neurospora cra... [more]
IF4A2_RAT1.352e-5668.52Eukaryotic initiation factor 4A-II OS=Rattus norve... [more]
IF4A2_PONAB1.352e-5668.52Eukaryotic initiation factor 4A-II OS=Pongo abelii... [more]
IF4A2_MOUSE1.352e-5668.52Eukaryotic initiation factor 4A-II OS=Mus musculus... [more]
IF4A2_HUMAN1.352e-5668.52Eukaryotic initiation factor 4A-II OS=Homo sapiens... [more]
IF4A2_BOVIN1.352e-5668.52Eukaryotic initiation factor 4A-II OS=Bos taurus G... [more]
IF4A_PHANO1.766e-5666.46ATP-dependent RNA helicase eIF4A OS=Phaeosphaeria ... [more]
IF4A_NEOFI1.766e-5666.46ATP-dependent RNA helicase eIF4A OS=Neosartorya fi... [more]
IF4A_COCIM3.012e-5666.46ATP-dependent RNA helicase eIF4A OS=Coccidioides i... [more]
IF4A_ASPOR3.012e-5661.36ATP-dependent RNA helicase eIF4A OS=Aspergillus or... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS06_0001N15_r Washington Navel Orange Stored Fruit Rind cDNA Library Citrus sinensis cDNA clone CS_WEc0001N15, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN189435 ID=CN189435; Name=CN189435; organism=Citrus sinensis; type=EST; length=709bp
GCAGGCGAGGAGTTTGGTAACCCTAGCAACTATTTTCTCTTAAATCAATC
TTCTCCTTCTCTTTGAAAAAAAAAAGAAAACCAACTCTTTCTCTCCATTT
TAATCACCATCATCAAAGCTTTGCAGATATGGCTGGTTTGGCACCCGAAG
GATCTCAATTTGATGCTCGTCAGTATGACACCAAGATGAATGAACTGCTC
TCTACTGATGGGCAAGATTTCTTCACTTCTTATGATGAAGTTTATGACAG
TTTTGATGCAATGGGACTGCAGGAAAACCTTTTGAGGGGAATCTATGCTT
ATGGGTTTGAGAAGCCTTCTGCTATTCAGCAAAGGGGAATTGTTCCCTTC
TGCAAGGGACTTGACGTCATTCAGCAGGCTCAGTCTGGAACTGGGAAAAC
AGCAACTTTCTGCTCTGGAATTTTGCAGCAACTTGATTATGGTCTTGTCC
AATGTCAGGCCCTGGTGTTGGCACCCACTAGGGAACTAGCTCAACAAATT
GAGAAAGTAATGAGGGCACTTGGTGATTATCTTGGTGTTAAGGTTCATGC
TTGTGTCGGTGGGACAAGTGTTCGCGAGGATCAGCGCATTCTTCAAGCTG
GTGTTCATGTTGTTGTTGGCACTCCTGGTCGTGTGTTTGACATGCTGCGC
CGACAGTCTTTACGCCCTGATTACATTAAGATGTTTGTACTGGATGAGGC
TGATGAAAT
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