EY756823

Overview
NameEY756823
Unique NameEY756823
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length914
Libraries
Library NameType
Sweet orange leaf, field plant Bcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig12734 contig Csv1_Contig12734:353..1267. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY756823 vs. ExPASy Swiss-Prot
Match: GLYA_ECOUT (Serine hydroxymethyltransferase OS=Escherichia coli (strain UTI89 / UPEC) GN=glyA PE=3 SV=2)

HSP 1 Score: 221.09 bits (562), Expect = 7.071e-57
Identity = 118/259 (45.56%), Postives = 162/259 (62.55%), Query Frame = 2 
Query:   23 GHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYEQLEKSAALFRPKLIVAGASAYARLYDYARIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRKGVKEINKQGQEVMYAYEDKINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMGPGGHRMGT 796
            GHL+HG       ++     +  +PY +D +TG+IDY  LEK A   +PK+I+ G SAY+ + D+A++R++ D   A +  DMAH++GLVAAGV P+P  +A VVTTTTHK+L GPRG +I  + G +E+ K           K+N AVFPG QGGP  H I+G AVALK+   PE+K YQ+QV  N+    +  LERGY +VSGGT+NHL LV+L +K + G   +  L   +I  NKN+VP D  S     G R+GT
Sbjct:  125 GHLTHG-----SPVNFSGKLYNIVPYGID-ATGHIDYADLEKQAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLILAKGGSEELYK-----------KLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRVGT 366
[Close]
BLAST of EY756823 vs. ExPASy Swiss-Prot
Match: GLYA_ECOSM (Serine hydroxymethyltransferase OS=Escherichia coli (strain SMS-3-5 / SECEC) GN=glyA PE=3 SV=1)

HSP 1 Score: 221.09 bits (562), Expect = 7.071e-57
Identity = 118/259 (45.56%), Postives = 162/259 (62.55%), Query Frame = 2 
Query:   23 GHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYEQLEKSAALFRPKLIVAGASAYARLYDYARIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRKGVKEINKQGQEVMYAYEDKINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMGPGGHRMGT 796
            GHL+HG       ++     +  +PY +D +TG+IDY  LEK A   +PK+I+ G SAY+ + D+A++R++ D   A +  DMAH++GLVAAGV P+P  +A VVTTTTHK+L GPRG +I  + G +E+ K           K+N AVFPG QGGP  H I+G AVALK+   PE+K YQ+QV  N+    +  LERGY +VSGGT+NHL LV+L +K + G   +  L   +I  NKN+VP D  S     G R+GT
Sbjct:  125 GHLTHG-----SPVNFSGKLYNIVPYGID-ATGHIDYADLEKQAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLILAKGGSEELYK-----------KLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRVGT 366
[Close]
BLAST of EY756823 vs. ExPASy Swiss-Prot
Match: GLYA_ECOSE (Serine hydroxymethyltransferase OS=Escherichia coli (strain SE11) GN=glyA PE=3 SV=1)

HSP 1 Score: 221.09 bits (562), Expect = 7.071e-57
Identity = 118/259 (45.56%), Postives = 162/259 (62.55%), Query Frame = 2 
Query:   23 GHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYEQLEKSAALFRPKLIVAGASAYARLYDYARIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRKGVKEINKQGQEVMYAYEDKINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMGPGGHRMGT 796
            GHL+HG       ++     +  +PY +D +TG+IDY  LEK A   +PK+I+ G SAY+ + D+A++R++ D   A +  DMAH++GLVAAGV P+P  +A VVTTTTHK+L GPRG +I  + G +E+ K           K+N AVFPG QGGP  H I+G AVALK+   PE+K YQ+QV  N+    +  LERGY +VSGGT+NHL LV+L +K + G   +  L   +I  NKN+VP D  S     G R+GT
Sbjct:  125 GHLTHG-----SPVNFSGKLYNIVPYGID-ATGHIDYADLEKQAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLILAKGGSEELYK-----------KLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRVGT 366
[Close]
BLAST of EY756823 vs. ExPASy Swiss-Prot
Match: GLYA_ECOLI (Serine hydroxymethyltransferase OS=Escherichia coli (strain K12) GN=glyA PE=1 SV=1)

HSP 1 Score: 221.09 bits (562), Expect = 7.071e-57
Identity = 118/259 (45.56%), Postives = 162/259 (62.55%), Query Frame = 2 
Query:   23 GHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYEQLEKSAALFRPKLIVAGASAYARLYDYARIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRKGVKEINKQGQEVMYAYEDKINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMGPGGHRMGT 796
            GHL+HG       ++     +  +PY +D +TG+IDY  LEK A   +PK+I+ G SAY+ + D+A++R++ D   A +  DMAH++GLVAAGV P+P  +A VVTTTTHK+L GPRG +I  + G +E+ K           K+N AVFPG QGGP  H I+G AVALK+   PE+K YQ+QV  N+    +  LERGY +VSGGT+NHL LV+L +K + G   +  L   +I  NKN+VP D  S     G R+GT
Sbjct:  125 GHLTHG-----SPVNFSGKLYNIVPYGID-ATGHIDYADLEKQAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLILAKGGSEELYK-----------KLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRVGT 366
[Close]
BLAST of EY756823 vs. ExPASy Swiss-Prot
Match: GLYA_ECOLC (Serine hydroxymethyltransferase OS=Escherichia coli (strain ATCC 8739 / DSM 1576 / Crooks) GN=glyA PE=3 SV=1)

HSP 1 Score: 221.09 bits (562), Expect = 7.071e-57
Identity = 118/259 (45.56%), Postives = 162/259 (62.55%), Query Frame = 2 
Query:   23 GHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYEQLEKSAALFRPKLIVAGASAYARLYDYARIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRKGVKEINKQGQEVMYAYEDKINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMGPGGHRMGT 796
            GHL+HG       ++     +  +PY +D +TG+IDY  LEK A   +PK+I+ G SAY+ + D+A++R++ D   A +  DMAH++GLVAAGV P+P  +A VVTTTTHK+L GPRG +I  + G +E+ K           K+N AVFPG QGGP  H I+G AVALK+   PE+K YQ+QV  N+    +  LERGY +VSGGT+NHL LV+L +K + G   +  L   +I  NKN+VP D  S     G R+GT
Sbjct:  125 GHLTHG-----SPVNFSGKLYNIVPYGID-ATGHIDYADLEKQAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLILAKGGSEELYK-----------KLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRVGT 366
[Close]
BLAST of EY756823 vs. ExPASy Swiss-Prot
Match: GLYA_ECOL6 (Serine hydroxymethyltransferase OS=Escherichia coli O6 GN=glyA PE=3 SV=1)

HSP 1 Score: 221.09 bits (562), Expect = 7.071e-57
Identity = 118/259 (45.56%), Postives = 162/259 (62.55%), Query Frame = 2 
Query:   23 GHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYEQLEKSAALFRPKLIVAGASAYARLYDYARIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRKGVKEINKQGQEVMYAYEDKINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMGPGGHRMGT 796
            GHL+HG       ++     +  +PY +D +TG+IDY  LEK A   +PK+I+ G SAY+ + D+A++R++ D   A +  DMAH++GLVAAGV P+P  +A VVTTTTHK+L GPRG +I  + G +E+ K           K+N AVFPG QGGP  H I+G AVALK+   PE+K YQ+QV  N+    +  LERGY +VSGGT+NHL LV+L +K + G   +  L   +I  NKN+VP D  S     G R+GT
Sbjct:  125 GHLTHG-----SPVNFSGKLYNIVPYGID-ATGHIDYADLEKQAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLILAKGGSEELYK-----------KLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRVGT 366
[Close]
BLAST of EY756823 vs. ExPASy Swiss-Prot
Match: GLYA_ECOL5 (Serine hydroxymethyltransferase OS=Escherichia coli O6:K15:H31 (strain 536 / UPEC) GN=glyA PE=3 SV=1)

HSP 1 Score: 221.09 bits (562), Expect = 7.071e-57
Identity = 118/259 (45.56%), Postives = 162/259 (62.55%), Query Frame = 2 
Query:   23 GHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYEQLEKSAALFRPKLIVAGASAYARLYDYARIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRKGVKEINKQGQEVMYAYEDKINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMGPGGHRMGT 796
            GHL+HG       ++     +  +PY +D +TG+IDY  LEK A   +PK+I+ G SAY+ + D+A++R++ D   A +  DMAH++GLVAAGV P+P  +A VVTTTTHK+L GPRG +I  + G +E+ K           K+N AVFPG QGGP  H I+G AVALK+   PE+K YQ+QV  N+    +  LERGY +VSGGT+NHL LV+L +K + G   +  L   +I  NKN+VP D  S     G R+GT
Sbjct:  125 GHLTHG-----SPVNFSGKLYNIVPYGID-ATGHIDYADLEKQAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLILAKGGSEELYK-----------KLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRVGT 366
[Close]
BLAST of EY756823 vs. ExPASy Swiss-Prot
Match: GLYA_ECOK1 (Serine hydroxymethyltransferase OS=Escherichia coli O1:K1 / APEC GN=glyA PE=3 SV=2)

HSP 1 Score: 221.09 bits (562), Expect = 7.071e-57
Identity = 118/259 (45.56%), Postives = 162/259 (62.55%), Query Frame = 2 
Query:   23 GHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYEQLEKSAALFRPKLIVAGASAYARLYDYARIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRKGVKEINKQGQEVMYAYEDKINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMGPGGHRMGT 796
            GHL+HG       ++     +  +PY +D +TG+IDY  LEK A   +PK+I+ G SAY+ + D+A++R++ D   A +  DMAH++GLVAAGV P+P  +A VVTTTTHK+L GPRG +I  + G +E+ K           K+N AVFPG QGGP  H I+G AVALK+   PE+K YQ+QV  N+    +  LERGY +VSGGT+NHL LV+L +K + G   +  L   +I  NKN+VP D  S     G R+GT
Sbjct:  125 GHLTHG-----SPVNFSGKLYNIVPYGID-ATGHIDYADLEKQAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLILAKGGSEELYK-----------KLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRVGT 366
[Close]
BLAST of EY756823 vs. ExPASy Swiss-Prot
Match: GLYA_ECOHS (Serine hydroxymethyltransferase OS=Escherichia coli O9:H4 (strain HS) GN=glyA PE=3 SV=1)

HSP 1 Score: 221.09 bits (562), Expect = 7.071e-57
Identity = 118/259 (45.56%), Postives = 162/259 (62.55%), Query Frame = 2 
Query:   23 GHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYEQLEKSAALFRPKLIVAGASAYARLYDYARIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRKGVKEINKQGQEVMYAYEDKINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMGPGGHRMGT 796
            GHL+HG       ++     +  +PY +D +TG+IDY  LEK A   +PK+I+ G SAY+ + D+A++R++ D   A +  DMAH++GLVAAGV P+P  +A VVTTTTHK+L GPRG +I  + G +E+ K           K+N AVFPG QGGP  H I+G AVALK+   PE+K YQ+QV  N+    +  LERGY +VSGGT+NHL LV+L +K + G   +  L   +I  NKN+VP D  S     G R+GT
Sbjct:  125 GHLTHG-----SPVNFSGKLYNIVPYGID-ATGHIDYADLEKQAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLILAKGGSEELYK-----------KLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRVGT 366
[Close]
BLAST of EY756823 vs. ExPASy Swiss-Prot
Match: GLYA_ECODH (Serine hydroxymethyltransferase OS=Escherichia coli (strain K12 / DH10B) GN=glyA PE=3 SV=1)

HSP 1 Score: 221.09 bits (562), Expect = 7.071e-57
Identity = 118/259 (45.56%), Postives = 162/259 (62.55%), Query Frame = 2 
Query:   23 GHLSHGYQTDTKKISAVSIFFETMPYRLDESTGYIDYEQLEKSAALFRPKLIVAGASAYARLYDYARIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRKGVKEINKQGQEVMYAYEDKINQAVFPGLQGGPHNHTISGLAVALKQVKTPEYKAYQEQVLSNSSKFAQSLLERGYDLVSGGTENHLVLVNLRNKGIDGSRVEKVLESVHIAANKNTVPGD-VSAMGPGGHRMGT 796
            GHL+HG       ++     +  +PY +D +TG+IDY  LEK A   +PK+I+ G SAY+ + D+A++R++ D   A +  DMAH++GLVAAGV P+P  +A VVTTTTHK+L GPRG +I  + G +E+ K           K+N AVFPG QGGP  H I+G AVALK+   PE+K YQ+QV  N+    +  LERGY +VSGGT+NHL LV+L +K + G   +  L   +I  NKN+VP D  S     G R+GT
Sbjct:  125 GHLTHG-----SPVNFSGKLYNIVPYGID-ATGHIDYADLEKQAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVPHAHVVTTTTHKTLAGPRGGLILAKGGSEELYK-----------KLNSAVFPGGQGGPLMHVIAGKAVALKEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEADAALGRANITVNKNSVPNDPKSPFVTSGIRVGT 366
The following BLAST results are available for this feature:
BLAST of EY756823 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
GLYA_ECOUT7.071e-5745.56Serine hydroxymethyltransferase OS=Escherichia col... [more]
GLYA_ECOSM7.071e-5745.56Serine hydroxymethyltransferase OS=Escherichia col... [more]
GLYA_ECOSE7.071e-5745.56Serine hydroxymethyltransferase OS=Escherichia col... [more]
GLYA_ECOLI7.071e-5745.56Serine hydroxymethyltransferase OS=Escherichia col... [more]
GLYA_ECOLC7.071e-5745.56Serine hydroxymethyltransferase OS=Escherichia col... [more]
GLYA_ECOL67.071e-5745.56Serine hydroxymethyltransferase OS=Escherichia col... [more]
GLYA_ECOL57.071e-5745.56Serine hydroxymethyltransferase OS=Escherichia col... [more]
GLYA_ECOK17.071e-5745.56Serine hydroxymethyltransferase OS=Escherichia col... [more]
GLYA_ECOHS7.071e-5745.56Serine hydroxymethyltransferase OS=Escherichia col... [more]
GLYA_ECODH7.071e-5745.56Serine hydroxymethyltransferase OS=Escherichia col... [more]

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Properties
Property NameValue
Genbank descriptionCS13-C1-001-014-A10-CT.F Sweet orange leaf, field plant B Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY756823 ID=EY756823; Name=EY756823; organism=Citrus sinensis; type=EST; length=914bp
TTGCGGTCGACCCACGCGTCCGGGACATCTTTCACATGGTTATCAGACTG
ACACCAAGAAGATATCTGCTGTGTCCATATTCTTTGAGACAATGCCATAC
AGGTTGGATGAGAGCACAGGATATATTGATTACGAACAGTTGGAGAAAAG
TGCTGCACTTTTCAGACCAAAACTCATAGTTGCAGGTGCAAGTGCTTATG
CTCGCCTCTATGATTATGCTCGTATTCGCAAGGTCTGTGACAAGCAGAAA
GCTGTTATGTTGGCTGATATGGCACACATTAGTGGGTTGGTTGCTGCTGG
TGTTATTCCATCTCCATTTGAATATGCAGATGTTGTGACGACCACAACAC
ACAAGTCACTCCGTGGTCCACGTGGTGCCATGATCTTCTTCAGGAAGGGG
GTTAAAGAGATTAATAAACAAGGACAAGAAGTGATGTATGCCTATGAAGA
CAAAATTAATCAGGCAGTTTTCCCTGGACTTCAAGGTGGTCCACATAATC
ACACCATATCAGGATTAGCTGTCGCACTTAAGCAGGTAAAGACCCCTGAA
TACAAGGCCTACCAGGAGCAAGTTCTCAGCAATTCTTCAAAGTTTGCACA
GAGTTTGCTAGAGAGAGGTTATGATCTGGTATCTGGTGGAACTGAGAACC
ATCTAGTCTTGGTGAACTTGAGAAACAAGGGTATTGACGGATCAAGAGTT
GAAAAAGTTTTGGAATCAGTTCATATTGCAGCCAACAAAAACACTGTTCC
TGGTGACGTGTCTGCTATGGGTCCTGGTGGNCATCGCATGGGAACCCAGC
CCTCACATCTAGGGAGTTTGNTGAGGAAAGATTTGAAAAGTCGCTGAGTT
CTTCATGCAGCTGTGAAGTGGCATTAAGATCAGGGTGATACTAAGGAACA
AGTGAAGATTTGTA
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