EY756690 | Citrus Genome Database

Overview


Name	EY756690
Unique Name	EY756690
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	896

Libraries


Library Name	Type
Sweet orange leaf, field plant B	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig5507	contig	Csv1_Contig5507:167..1065.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY756690 vs. ExPASy Swiss-Prot
Match: ALF1_XANCP (Probable fructose-bisphosphate aldolase class 1 OS=Xanthomonas campestris pv. campestris GN=XCC3185 PE=3 SV=1)

HSP 1 Score: 172.17 bits (435), Expect = 5.666e-45
Identity = 86/166 (51.81%), Postives = 112/166 (67.47%), Query Frame = 3

Query:  255 DELVKTAKTVASPGRGILAMDESNATCGKRLASIGLENTEANRQAYRTLLVTAPGLGQYISGAILFEETLYQSTTDGKKMVDVLVEQNIVPGIKVDKGLVPLAGSNDESWCQGLDGLASRTAAYYQQGARFAKWRTVVSIPNG-PSALAVREAAWGLARYAAIAQE 749
            ++L +TA+ + +PG+GI+A+DES +T  KR + +G+ENTE NR+AYR LL+T P L  YISGAILF+ET+ Q T DG      + +  I+PGIKVDKG  PLAG   E   +GLDGL +R   YY  GARFAKWR V++I    PS   +   A  LARYAA+ QE
Sbjct:    4 EQLAETAQAMVAPGKGIIAIDESTSTIAKRFSGVGIENTEENRRAYRELLLTTPKLSDYISGAILFDETIRQKTKDGVPFAKYMADHGIIPGIKVDKGAQPLAGMPGELVTEGLDGLRARLEEYYTLGARFAKWRAVINIGEDIPSGTCIEANAHALARYAALCQE 169

HSP 2 Score: 24.6386 bits (52), Expect = 5.666e-45
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 2

Query:  740 CSRNGLVPIVEPE 778
            C   GLVP+VEPE
Sbjct:  167 CQEQGLVPMVEPE 179

HSP 3 Score: 24.6386 bits (52), Expect = 5.666e-45
Identity = 12/42 (28.57%), Postives = 22/42 (52.38%), Query Frame = 1

Query:  760 PNCGARILLDGDHGIDRTFEVAQKVWAEVSSTC*E-HVMVEG 882
            P     +++DGDH I+  +EV +     +     E +V++EG
Sbjct:  174 PMVEPEVIMDGDHDIETCYEVTEATLRSLFGALYEQNVVLEG 215

[Close]

BLAST of EY756690 vs. ExPASy Swiss-Prot
Match: ALF2_PLABA (Fructose-bisphosphate aldolase 2 OS=Plasmodium berghei (strain Anka) GN=ALDO2 PE=3 SV=1)

HSP 1 Score: 173.326 bits (438), Expect = 2.300e-44
Identity = 90/169 (53.25%), Postives = 118/169 (69.82%), Query Frame = 3

Query:  252 ADELVKTAKTVASPGRGILAMDESNATCGKRLASIGLENTEANRQAYRTLLVTAPGLGQYISGAILFEETLYQSTTDGKKMVDVLVEQNIVPGIKVDKGLVPLAGSNDESWCQGLDGLASRTAAYYQQGARFAKWRTVVSI---PNGPSALAVREAAWGLARYAAIAQE 749
            A EL +TAK + + G+GILA DES  T  KR  +I +ENT  NR +YR LL    GLG++ISGAILFEETL+Q    G  +V++L ++ I+PGIKVDKGLV +  ++DE   QGLDGLA R   YY+ GARFAKWR V+ I      P+ L+++E +WGLARYA+I Q+
Sbjct:    8 AQELAETAKKLVAAGKGILAADESTQTIKKRFDNIKIENTVQNRASYRDLLFGTKGLGKFISGAILFEETLFQKNEAGVPLVNLLHDEGIIPGIKVDKGLVSIPCTDDEKSTQGLDGLAERCKEYYKAGARFAKWRAVLVIDPAKGKPTDLSIQEVSWGLARYASICQQ 176

HSP 2 Score: 25.409 bits (54), Expect = 2.300e-44
Identity = 10/13 (76.92%), Postives = 11/13 (84.62%), Query Frame = 2

Query:  740 CSRNGLVPIVEPE 778
            C +N LVPIVEPE
Sbjct:  174 CQQNKLVPIVEPE 186

[Close]

BLAST of EY756690 vs. ExPASy Swiss-Prot
Match: ALF1_XANAC (Probable fructose-bisphosphate aldolase class 1 OS=Xanthomonas axonopodis pv. citri GN=XAC3344 PE=3 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 4.638e-44
Identity = 86/166 (51.81%), Postives = 112/166 (67.47%), Query Frame = 3

Query:  255 DELVKTAKTVASPGRGILAMDESNATCGKRLASIGLENTEANRQAYRTLLVTAPGLGQYISGAILFEETLYQSTTDGKKMVDVLVEQNIVPGIKVDKGLVPLAGSNDESWCQGLDGLASRTAAYYQQGARFAKWRTVVSIPNG-PSALAVREAAWGLARYAAIAQE 749
            ++L +TA+ + +PG+GI+A+DES +T  KR AS+G+EN E NR+AYR LL+T P L  YISGAILF+ET+ Q T DG    + +    I+PGIKVDKG  PLAG   E   +GLDGL +R   YY  GARFAKWR V++I    PS   +   +  LARYAA+ QE
Sbjct:    4 EQLAETAQAMVAPGKGIIAIDESTSTIAKRFASVGIENIEENRRAYRELLLTTPKLSDYISGAILFDETIRQKTKDGVPFAEYMTAHGIIPGIKVDKGAQPLAGMPGELVTEGLDGLRARLEEYYTLGARFAKWRAVINIGEDIPSGTCIEANSHALARYAALCQE 169

HSP 2 Score: 24.6386 bits (52), Expect = 4.638e-44
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 2

Query:  740 CSRNGLVPIVEPE 778
            C   GLVP+VEPE
Sbjct:  167 CQEQGLVPMVEPE 179

HSP 3 Score: 22.3274 bits (46), Expect = 4.638e-44
Identity = 11/42 (26.19%), Postives = 21/42 (50.00%), Query Frame = 1

Query:  760 PNCGARILLDGDHGIDRTFEVAQKVWAEVSSTC*E-HVMVEG 882
            P     +++DG H I+  +EV +     +     E +V++EG
Sbjct:  174 PMVEPEVIMDGSHDIETCYEVTEATLRSLFGALYEQNVVLEG 215

[Close]

BLAST of EY756690 vs. ExPASy Swiss-Prot
Match: ALF2_CAEEL (Fructose-bisphosphate aldolase 2 OS=Caenorhabditis elegans GN=aldo-2 PE=1 SV=1)

HSP 1 Score: 168.703 bits (426), Expect = 1.126e-43
Identity = 92/165 (55.76%), Postives = 113/165 (68.48%), Query Frame = 3

Query:  255 DELVKTAKTVASPGRGILAMDESNATCGKRLASIGLENTEANRQAYRTLLVTAPGLGQYISGAILFEETLYQSTTDGKKMVDVLVEQNIVPGIKVDKGLVPLAGSNDESWCQGLDGLASRTAAYYQQGARFAKWRTVVSI-PNGPSALAVREAAWGLARYAAIAQ 746
            DEL + A  +   G+GILA DES  T GKRL +I LEN E NRQ YR LL T P L Q+ISG IL+EET +QST  G+K  D+L++Q IVPGIK+D G+VPLAG+  E   QGLD LA R AA+ + G  FAKWR V++I  + PS L + E A  LARYA+I Q
Sbjct:   18 DELHQIALKIVQDGKGILAADESTGTIGKRLDAINLENNETNRQKYRQLLFTTPNLNQHISGVILYEETFHQSTDKGEKFTDLLIKQGIVPGIKLDLGVVPLAGTIGEGTTQGLDKLAERAAAFKKGGCGFAKWRCVLNIGTHTPSHLGMLENANVLARYASICQ 182

HSP 2 Score: 27.7202 bits (60), Expect = 1.126e-43
Identity = 11/13 (84.62%), Postives = 11/13 (84.62%), Query Frame = 2

Query:  740 CSRNGLVPIVEPE 778
            C  NGLVPIVEPE
Sbjct:  181 CQANGLVPIVEPE 193

[Close]

BLAST of EY756690 vs. ExPASy Swiss-Prot
Match: ALF_DROME (Fructose-bisphosphate aldolase OS=Drosophila melanogaster GN=Ald PE=1 SV=5)

HSP 1 Score: 164.081 bits (414), Expect = 4.233e-43
Identity = 92/166 (55.42%), Postives = 112/166 (67.47%), Query Frame = 3

Query:  255 DELVKTAKTVASPGRGILAMDESNATCGKRLASIGLENTEANRQAYRTLLV-TAPGLGQYISGAILFEETLYQSTTDGKKMVDVLVEQNIVPGIKVDKGLVPLAGSNDESWCQGLDGLASRTAAYYQQGARFAKWRTVVSI-PNGPSALAVREAAWGLARYAAIAQ 746
            DEL + A+ + +PG+GILA DES  T GKRL  IG+ENTE NR+AYR LL  T P L + ISG ILF ETLYQ   DG    ++L ++ I+ GIKVDKG+VPL GS DE   QGLD LA+R A Y + G  FAKWR V+ I  N PS  ++ E A  LARYA+I Q
Sbjct:   14 DELREIAQKIVAPGKGILAADESGPTMGKRLQDIGVENTEDNRRAYRQLLFSTDPKLAENISGVILFHETLYQKADDGTPFAEILKKKGIILGIKVDKGVVPLFGSEDEVTTQGLDDLAARCAQYKKDGCDFAKWRCVLKIGKNTPSYQSILENANVLARYASICQ 179

HSP 2 Score: 30.4166 bits (67), Expect = 4.233e-43
Identity = 18/46 (39.13%), Postives = 25/46 (54.35%), Query Frame = 1

Query:  748 KRIGPNCGARILLDGDHGIDRTFEVAQKVWAEV-SSTC*EHVMVEG 882
            +RI P     +L DGDH +DR  +V + V A V  +    HV +EG
Sbjct:  181 QRIVPIVEPEVLPDGDHDLDRAQKVTETVLAAVYKALSDHHVYLEG 226

[Close]

BLAST of EY756690 vs. ExPASy Swiss-Prot
Match: ALF1_XYLFT (Probable fructose-bisphosphate aldolase class 1 OS=Xylella fastidiosa (strain Temecula1 / ATCC 700964) GN=PD_1845 PE=3 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 1.085e-42
Identity = 85/166 (51.20%), Postives = 112/166 (67.47%), Query Frame = 3

Query:  255 DELVKTAKTVASPGRGILAMDESNATCGKRLASIGLENTEANRQAYRTLLVTAPGLGQYISGAILFEETLYQSTTDGKKMVDVLVEQNIVPGIKVDKGLVPLAGSNDESWCQGLDGLASRTAAYYQQGARFAKWRTVVSIPNG-PSALAVREAAWGLARYAAIAQE 749
            ++L +TA+ + + G+GI+A+DES  T  KR +S+G+EN E NR+AYR LL+T P L  YISGAILF+ET+ QST  G      + E  I+PGIKVDKG  PLAG   E   +GLDGL +R   YY+ GARFAKWR V++I +  PS + +      LARYAA+ QE
Sbjct:    4 EQLAETAQAMVASGKGIIAIDESAGTIAKRFSSVGIENIEENRRAYRELLLTTPKLSDYISGAILFDETIRQSTKAGVPFPKYMAEHGIIPGIKVDKGAYPLAGCPGELVTEGLDGLRARLEEYYKLGARFAKWRAVINIGDDIPSGMCIDANVHALARYAALCQE 169

HSP 2 Score: 24.6386 bits (52), Expect = 1.085e-42
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 2

Query:  740 CSRNGLVPIVEPE 778
            C   GLVP+VEPE
Sbjct:  167 CQEQGLVPMVEPE 179

HSP 3 Score: 22.7126 bits (47), Expect = 1.085e-42
Identity = 11/42 (26.19%), Postives = 22/42 (52.38%), Query Frame = 1

Query:  760 PNCGARILLDGDHGIDRTFEVAQKVWAEVSSTC*E-HVMVEG 882
            P     +++DG+H I   +EV +     + +   E +V++EG
Sbjct:  174 PMVEPEVIMDGNHDISAAYEVTEATLRSLFNALYEQNVVLEG 215

[Close]

BLAST of EY756690 vs. ExPASy Swiss-Prot
Match: ALF2_LAMJA (Fructose-bisphosphate aldolase, non-muscle type OS=Lampetra japonica PE=2 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 2.072e-42
Identity = 90/166 (54.22%), Postives = 115/166 (69.28%), Query Frame = 3

Query:  258 ELVKTAKTVASPGRGILAMDESNATCGKRLASIGLENTEANRQAYRTLLVTA-PGLGQYISGAILFEETLYQSTTDGKKMVDVLVEQNIVPGIKVDKGLVPLAGSNDESWCQGLDGLASRTAAYYQQGARFAKWRTVVSI-PNGPSALAVREAAWGLARYAAIAQE 749
            EL + A+ + S G+GILA DES  T GKRL++I +EN + NR+ YR LL ++ P + + I G I +EETLYQ T DG   V ++ ++ IV GIKVDKG VPLAG++ ES  QGLDGLA R A Y + GA FAKWR V+ I  N PSALA+ E A  LARYA+I Q+
Sbjct:   15 ELAEIAQRIVSNGKGILAADESTGTMGKRLSAIKVENVDENRRVYRQLLFSSDPSVTKAIGGVIFYEETLYQKTDDGTPFVKLIKDRGIVVGIKVDKGTVPLAGTDGESTTQGLDGLAERCARYKKDGADFAKWRCVLKISKNTPSALAIAENANVLARYASICQQ 180

HSP 2 Score: 28.4906 bits (62), Expect = 2.072e-42
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 2

Query:  740 CSRNGLVPIVEPE 778
            C +NGLVPIVEPE
Sbjct:  178 CQQNGLVPIVEPE 190

[Close]

BLAST of EY756690 vs. ExPASy Swiss-Prot
Match: ALF1_XYLFA (Probable fructose-bisphosphate aldolase class 1 OS=Xylella fastidiosa GN=XF_0826 PE=3 SV=1)

HSP 1 Score: 165.236 bits (417), Expect = 2.386e-42
Identity = 84/166 (50.60%), Postives = 112/166 (67.47%), Query Frame = 3

Query:  255 DELVKTAKTVASPGRGILAMDESNATCGKRLASIGLENTEANRQAYRTLLVTAPGLGQYISGAILFEETLYQSTTDGKKMVDVLVEQNIVPGIKVDKGLVPLAGSNDESWCQGLDGLASRTAAYYQQGARFAKWRTVVSIPNG-PSALAVREAAWGLARYAAIAQE 749
            ++L +TA+ + + G+GI+A+DES  T  KR +S+G+EN E NR+AYR LL+T P L  YISGAILF+ET+ QST  G      + +  I+PGIKVDKG  PLAG   E   +GLDGL +R   YY+ GARFAKWR V++I +  PS + +      LARYAA+ QE
Sbjct:    4 EQLAETAQAMVASGKGIIAIDESAGTIAKRFSSVGIENIEENRRAYRELLLTTPKLSDYISGAILFDETIRQSTKAGVPFPKYMADHGIIPGIKVDKGAYPLAGCPGELVTEGLDGLRARLEEYYKLGARFAKWRAVINIGDDIPSGMCIDANVHALARYAALCQE 169

HSP 2 Score: 24.6386 bits (52), Expect = 2.386e-42
Identity = 9/13 (69.23%), Postives = 10/13 (76.92%), Query Frame = 2

Query:  740 CSRNGLVPIVEPE 778
            C   GLVP+VEPE
Sbjct:  167 CQEQGLVPMVEPE 179

HSP 3 Score: 22.7126 bits (47), Expect = 2.386e-42
Identity = 11/42 (26.19%), Postives = 22/42 (52.38%), Query Frame = 1

Query:  760 PNCGARILLDGDHGIDRTFEVAQKVWAEVSSTC*E-HVMVEG 882
            P     +++DG+H I   +EV +     + +   E +V++EG
Sbjct:  174 PMVEPEVIMDGNHDISAAYEVTEATLRSLFNALYEQNVVLEG 215

[Close]

BLAST of EY756690 vs. ExPASy Swiss-Prot
Match: ALF1_LAMJA (Fructose-bisphosphate aldolase, muscle type OS=Lampetra japonica PE=2 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 2.244e-41
Identity = 88/166 (53.01%), Postives = 110/166 (66.27%), Query Frame = 3

Query:  258 ELVKTAKTVASPGRGILAMDESNATCGKRLASIGLENTEANRQAYRTLLVTA-PGLGQYISGAILFEETLYQSTTDGKKMVDVLVEQNIVPGIKVDKGLVPLAGSNDESWCQGLDGLASRTAAYYQQGARFAKWRTVVSI-PNGPSALAVREAAWGLARYAAIAQE 749
            EL   A+ + + G+GILA DES  T GKRL  IGLENT+ +R+ YR LL T  P + ++I G I F ET+YQ T  G   V ++ +  I+ GIKVDKG+VPLAG+N E   QGLDGLA R A Y + GA FAKWR V+ I PN PS L++ E A  LARYA I Q+
Sbjct:   15 ELADIAQRIVASGKGILAADESVGTMGKRLTQIGLENTDEHRRFYRQLLFTTDPSIKEHIGGIIFFHETMYQKTDGGVPFVKLVKDNGILVGIKVDKGVVPLAGTNGEGTTQGLDGLAERCAQYKKDGADFAKWRCVLKISPNTPSRLSIVENANVLARYATICQQ 180

HSP 2 Score: 28.4906 bits (62), Expect = 2.244e-41
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 2

Query:  740 CSRNGLVPIVEPE 778
            C +NGLVPIVEPE
Sbjct:  178 CQQNGLVPIVEPE 190

[Close]

BLAST of EY756690 vs. ExPASy Swiss-Prot
Match: ALDOA_RABIT (Fructose-bisphosphate aldolase A OS=Oryctolagus cuniculus GN=ALDOA PE=1 SV=2)

HSP 1 Score: 158.688 bits (400), Expect = 1.098e-40
Identity = 90/166 (54.22%), Postives = 110/166 (66.27%), Query Frame = 3

Query:  258 ELVKTAKTVASPGRGILAMDESNATCGKRLASIGLENTEANRQAYRTLLVTAPG-LGQYISGAILFEETLYQSTTDGKKMVDVLVEQNIVPGIKVDKGLVPLAGSNDESWCQGLDGLASRTAAYYQQGARFAKWRTVVSI-PNGPSALAVREAAWGLARYAAIAQE 749
            EL   A  + +PG+GILA DES  +  KRL SIG ENTE NR+ YR LL+TA   +   I G ILF ETLYQ   DG+    V+  +  V GIKVDKG+VPLAG+N E+  QGLDGL+ R A Y + GA FAKWR V+ I  + PSALA+ E A  LARYA+I Q+
Sbjct:   15 ELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQ 180

HSP 2 Score: 27.7202 bits (60), Expect = 1.098e-40
Identity = 10/13 (76.92%), Postives = 12/13 (92.31%), Query Frame = 2

Query:  740 CSRNGLVPIVEPE 778
            C +NG+VPIVEPE
Sbjct:  178 CQQNGIVPIVEPE 190

The following BLAST results are available for this feature:

BLAST of EY756690 vs. ExPASy Swiss-Prot

BLAST of EY756690 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 56


Match Name	E-value	Identity	Description
ALF1_XANCP	5.666e-45	51.81	Probable fructose-bisphosphate aldolase class 1 OS...	[more]
ALF2_PLABA	2.300e-44	53.25	Fructose-bisphosphate aldolase 2 OS=Plasmodium ber...	[more]
ALF1_XANAC	4.638e-44	51.81	Probable fructose-bisphosphate aldolase class 1 OS...	[more]
ALF2_CAEEL	1.126e-43	55.76	Fructose-bisphosphate aldolase 2 OS=Caenorhabditis...	[more]
ALF_DROME	4.233e-43	55.42	Fructose-bisphosphate aldolase OS=Drosophila melan...	[more]
ALF1_XYLFT	1.085e-42	51.20	Probable fructose-bisphosphate aldolase class 1 OS...	[more]
ALF2_LAMJA	2.072e-42	54.22	Fructose-bisphosphate aldolase, non-muscle type OS...	[more]
ALF1_XYLFA	2.386e-42	50.60	Probable fructose-bisphosphate aldolase class 1 OS...	[more]
ALF1_LAMJA	2.244e-41	53.01	Fructose-bisphosphate aldolase, muscle type OS=Lam...	[more]
ALDOA_RABIT	1.098e-40	54.22	Fructose-bisphosphate aldolase A OS=Oryctolagus cu...	[more]

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Properties


Property Name	Value
Genbank description	CS13-C1-001-011-E11-CT.F Sweet orange leaf, field plant B Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY756690 ID=EY756690; Name=EY756690; organism=Citrus sinensis; type=EST; length=896bp
CACGCGTCCGGAAGAAAATACCGAAGATAAGATTATAAGTGCTATAAGCT
AAGGCCTCTCGAGCTCAGAACCCACAGCACGAAGAAGCAAAGAGGAAAAT
GGCCTCGGCCTCTGCATCTTTACTCAAGTCATCATCCCCCGTTCTTGACA
AGTCTGAGTGGGTTAAGGGCCAGGCCATCCGCCAGTCTACTGTCTCAGTC
CGCAGCCTCCCCAGTGGCCCCTCTGCCCTCACCATCCGTGCTGGTTCCTA
TGCCGATGAGCTTGTCAAGACCGCGAAAACTGTTGCATCACCAGGACGTG
GAATTTTGGCCATGGATGAGTCAAATGCAACTTGTGGAAAACGCCTTGCC
TCAATTGGGTTAGAGAACACTGAGGCTAACCGCCAGGCATACCGTACCCT
CCTTGTTACAGCCCCTGGTCTTGGACAATACATCTCTGGTGCCATCCTTT
TTGAGGAGACTCTCTACCAATCCACCACCGATGGCAAGAAGATGGTTGAT
GTTCTTGTTGAGCAAAACATTGTCCCCGGTATCAAAGTCGACAAGGGTTT
GGTGCCCCTCGCTGGTTCCAATGACGAGTCATGGTGCCAGGGTCTTGATG
GCCTAGCTTCTCGCACTGCTGCTTACTACCAACAGGGAGCTCGTTTTGCC
AAATGGCGTACTGTTGTGAGCATTCCCAATGGCCCATCTGCTTTGGCAGT
CAGGGAAGCCGCCTGGGGTCTTGCCCGCTATGCTGCCATTGCTCAAGAAA
CGGATTGGTCCCAATTGTGGAGCCAGAATCTTGCTTGATGGTGATCATGG
CATTGACAGGACTTTTGAAGTAGCTCAGAAGGTTTGGGCTGAAGTTTCTT
CTACCTGCTGAGAACATGTCATGGTTGAGGGTATCTCCTCCAGCTA