CX288495

Overview
NameCX288495
Unique NameCX288495
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length594
Libraries
Library NameType
IF1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71BD_ARATH (Cytochrome P450 71B13 OS=Arabidopsis thaliana GN=CYP71B13 PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 3.823e-13
Identity = 38/100 (38.00%), Postives = 55/100 (55.00%), Query Frame = 2
Query:  296 EVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP--AGVHVEFY 589
            +++ AG DTS  T  W M HL+ N  + +K   E+   +     + EED+E + YL  VVKETLR +P    L    A KD ++GGY+IP    +HV  +
Sbjct:  293 DILLAGVDTSGHTITWVMTHLIKNPRVMKKAQAEVREVIKNKDNITEEDIEGLEYLKMVVKETLRINPLVPLLTPREASKDVKIGGYNIPKKTWIHVNIW 392          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71AN_ARATH (Cytochrome P450 71A23 OS=Arabidopsis thaliana GN=CYP71A23 PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 3.823e-13
Identity = 44/132 (33.33%), Postives = 65/132 (49.24%), Query Frame = 2
Query:  185 ESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            +++ V  + AA  D  FG +      +    I  +  +    GTDTS+T  EW M  L+ +    +KL +E+         V E+D++ M YL AVVKE LR HPP   ++ H + +D  L    IPAG  V
Sbjct:  254 KTDFVDVLLAAQRDKSFGFD------IDRLSIKAIVLDAFVGGTDTSSTLVEWEMTELLRHPTCLKKLQEEVRTICKGKSSVSEDDIQGMEYLKAVVKEALRLHPPVPLMVPHQSTQDVRLRDNHIPAGTQV 379          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A2_RABIT (Cytochrome P450 1A2 OS=Oryctolagus cuniculus GN=CYP1A2 PE=1 SV=3)

HSP 1 Score: 74.3294 bits (181), Expect = 4.993e-13
Identity = 51/177 (28.81%), Postives = 85/177 (48.02%), Query Frame = 2
Query:   44 DFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLG---EQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            DF PIL  L  R ++  K+  +R +  L   +R      ++    NS  ++    GA +  S    E   +   G   +++IV L +++  AG DT  T   W++ +LV N   Q K+ +E+ A VG+    +  D  ++PYL+A + E  R      F + H+  +DT L G+ IP
Sbjct:  237 DFFPILRYLPNRPLQRFKDFNQRFLRFLQKTVREHYEDFDR----NSIQDIT---GALFKHS----EKNSKANSGLIPQEKIVNLVNDIFGAGFDTITTALSWSLMYLVTNPRRQRKIQEELDAVVGRARQPRLSDRPQLPYLEAFILELFRHTSFVPFTIPHSTTRDTTLNGFHIP 402          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A1_MICTO (Cytochrome P450 1A1 OS=Microgadus tomcod GN=cyp1a1 PE=2 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 4.993e-13
Identity = 49/182 (26.92%), Postives = 85/182 (46.70%), Query Frame = 2
Query:   20 LITSPQLPDFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            ++ S  L DF+P+L  L    M+    + +R M  +  ++       +K    +    ++       +D    +      Q+ +++IV + +++  AG DT +T   W++ +LV + EIQE+L++EI   VG        D   +P L+A + E  R      F + H A KDT L GY IP
Sbjct:  228 VVGSGNLADFIPLLRFLPNATMKRFMAINERFMTFVQKIVTEHYNTYDKDNIRDITDSLIDHCEDRKLDENSNI------QMSDEKIVGIVNDLFGAGFDTVSTALSWSVMYLVAHPEIQERLHQEIKDKVGLSRSPVLTDRHNLPILEAFIFEIFRHSSFLPFTIPHCATKDTSLDGYFIP 403          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A1_CHACA (Cytochrome P450 1A1 OS=Chaetodon capistratus GN=cyp1a1 PE=2 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 4.993e-13
Identity = 38/102 (37.25%), Postives = 59/102 (57.84%), Query Frame = 2
Query:  260 QLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            Q+ +++IV + +++  AG DT +T   W++ +LV   +IQE+L++EI   VG D      D  K+PYL+A + E  R      F + H + KDT L GY IP
Sbjct:  306 QMSDEKIVGIVNDLFGAGFDTVSTALSWSVMYLVAYPDIQERLFQEIKDNVGLDRTPLLSDRSKVPYLEAFILELFRHSSFLPFTIPHCSAKDTSLNGYFIP 407          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C93B1_GLYEC (Licodione synthase OS=Glycyrrhiza echinata GN=CYP93B1 PE=1 SV=2)

HSP 1 Score: 74.3294 bits (181), Expect = 4.993e-13
Identity = 38/96 (39.58%), Postives = 57/96 (59.38%), Query Frame = 2
Query:  278 IVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            I  L  +  +AGTDT+A + EWA+  LV    + +K+ +EI   VGKD LV+E D   +PYL A++KET R HPP   +++   + +  +  Y IP
Sbjct:  294 IKALIMDFFTAGTDTTAISTEWALVELVKKPSVLQKVREEIDNVVGKDRLVEESDCPNLPYLQAILKETFRLHPPVP-MVTRRCVAECTVENYVIP 388          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C75A7_EUSGR (Flavonoid 3',5'-hydroxylase OS=Eustoma grandiflorum GN=CYP75A7 PE=2 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 4.993e-13
Identity = 38/108 (35.19%), Postives = 58/108 (53.70%), Query Frame = 2
Query:  257 EQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            E+L    I  L   + +AGTDTS++  EWA+  L+ N  I  +  +E+   +G+D    E D+ K+PYL A+ KE  R+HP     L   A +  E+ G+ IP G  +
Sbjct:  288 ERLQTVNIKALLLNMFTAGTDTSSSVIEWALAELLKNPIILRRAQEEMDGVIGRDRRFLEADISKLPYLQAICKEAFRKHPSTPLNLPRIASQACEVNGHYIPKGTRL 395          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A5_CHICK (Cytochrome P450 1A5 OS=Gallus gallus GN=CYP1A5 PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 6.521e-13
Identity = 46/174 (26.44%), Postives = 81/174 (46.55%), Query Frame = 2
Query:   44 DFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            DF+P+L  L  R M    +  KR M+ L   +       +K    N+  ++   +    V+     E  G  Q+  ++I+ L +++  AG DT  T   W++ +LV    +Q+K+  E+   +G++   +  D   +PY +A + E  R      F + H+  +DT L GY IP
Sbjct:  244 DFIPLLRYLPSRNMDSFLDFNKRFMKLLQTAVEEHYQTFDK----NNIRDVTDSLIEQCVEKK--AEANGATQIPNEKIINLVNDIFGAGFDTVTTALSWSLMYLVTYPHMQKKIQAELDQTIGRERRPRLSDRGMLPYTEAFILEMFRHSSFMPFTIPHSTTRDTVLNGYYIP 411          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A1_LIZSA (Cytochrome P450 1A1 OS=Liza saliens GN=cyp1a1 PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 6.521e-13
Identity = 46/174 (26.44%), Postives = 79/174 (45.40%), Query Frame = 2
Query:   44 DFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            DF+PIL  L  + M++   +  R +  +  ++    A   K    +    ++       +D           Q+ ++++V + +++  AG DT +T   W++ +LV   EIQE LY+E+   VG D      D   +P L++ + E  R      F + H   KDT L GY IP
Sbjct:  240 DFIPILQYLPNKTMKKFVSINDRFISFVQKIVSEHYATFNKDNIRDITDSLIDHCEDRKLDE------NANVQMSDEKVVGIVNDLFGAGFDTISTALSWSVMYLVAYPEIQEXLYQELKENVGLDRTPVLSDRNNLPLLESFILEIFRHSSFLPFTIPHCTTKDTSLNGYYIP 407          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71AP_ARATH (Cytochrome P450 71A25 OS=Arabidopsis thaliana GN=CYP71A25 PE=2 SV=1)

HSP 1 Score: 73.9442 bits (180), Expect = 6.521e-13
Identity = 42/126 (33.33%), Postives = 65/126 (51.59%), Query Frame = 2
Query:  209 GAAYVDSLFGL--EPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            G   +D+L  +  E +   ++    I  +  +V   G+DTS T  EWAM  L+ + +   +L +E+         V E+D++ M YL AV+KE LR HPP   +  H + +D +L  Y IPAG  V
Sbjct:  256 GTDLIDALLRVKREKSPGFEIERVSIKAITLDVFVGGSDTSFTLLEWAMTELLRHPKSLNRLQEEVRTICKGKSRVSEDDIQGMKYLKAVIKEALRLHPPFPMMAPHESTEDVKLRDYHIPAGTQV 381          
The following BLAST results are available for this feature:
BLAST of CX288495 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 193
Match NameE-valueIdentityDescription
C71BD_ARATH3.823e-1338.00Cytochrome P450 71B13 OS=Arabidopsis thaliana GN=C... [more]
C71AN_ARATH3.823e-1333.33Cytochrome P450 71A23 OS=Arabidopsis thaliana GN=C... [more]
CP1A2_RABIT4.993e-1328.81Cytochrome P450 1A2 OS=Oryctolagus cuniculus GN=CY... [more]
CP1A1_MICTO4.993e-1326.92Cytochrome P450 1A1 OS=Microgadus tomcod GN=cyp1a1... [more]
CP1A1_CHACA4.993e-1337.25Cytochrome P450 1A1 OS=Chaetodon capistratus GN=cy... [more]
C93B1_GLYEC4.993e-1339.58Licodione synthase OS=Glycyrrhiza echinata GN=CYP9... [more]
C75A7_EUSGR4.993e-1335.19Flavonoid 3',5'-hydroxylase OS=Eustoma grandifloru... [more]
CP1A5_CHICK6.521e-1326.44Cytochrome P450 1A5 OS=Gallus gallus GN=CYP1A5 PE=... [more]
CP1A1_LIZSA6.521e-1326.44Cytochrome P450 1A1 OS=Liza saliens GN=cyp1a1 PE=2... [more]
C71AP_ARATH6.521e-1333.33Cytochrome P450 71A25 OS=Arabidopsis thaliana GN=C... [more]

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Properties
Property NameValue
Genbank descriptionC02001A10SK IF1 Citrus clementina cDNA clone C02001A10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX288495 ID=CX288495; Name=CX288495; organism=Citrus clementina; type=EST; length=594bp
CATACTAAAAGATGTAATGCTGATAACATCACCACAGCTTCCGGATTTCT
TGCCGATTCTCACTCCATTATTTCGCAGGCAAATGAGAGAAGCAAAGGAG
TTGAGGAAGAGACAAATGGAGTGCTTGGTTCCACTGATAAGGAACAGAAA
AGCTTTTGTAGAGAAAGGCCAAAACCCTAACTCAGAGTCAGAGATGGTGA
GTCCAATTGGTGCTGCTTATGTGGATTCATTGTTTGGGCTGGAGCCAACG
GGGAGAGAGCAGCTGGGGGAACAAGAGATTGTGACATTGTGTTCTGAAGT
GATCAGTGCAGGCACTGACACTAGCGCAACAACGGCCGAATGGGCAATGC
ATCACTTGGTGCTAAACCAGGAGATTCAAGAAAAGTTGTATAAAGAAATT
GTAGCTGCTGTGGGGAAAGATGGGCTTGTTAAAGAAGAAGATGTCGAGAA
AATGCCTTATCTCGATGCTGTCGTGAAGGAAACTCTCCGGCGGCACCCTC
CTGGCCATTTTCTGCTGTCTCACGCTGCCATAAAGGATACTGAGCTTGGC
GGGTACTCCATCCCGGCAGGTGTTCACGTGGAATTCTACACTGC
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