CX288495

Overview
NameCX288495
Unique NameCX288495
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length594
Libraries
Library NameType
IF1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71C4_MAIZE (Cytochrome P450 71C4 OS=Zea mays GN=CYP71C4 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.453e-12
Identity = 54/166 (32.53%), Postives = 86/166 (51.81%), Query Frame = 2
Query:   89 EAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAV--GKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            + + +RKR  + L  LI +  A +   Q+ + + + +      YV  L  L+      L    I  +  ++  AGTDTS  T E+AM  L+    + +KL +E+   V  G++ +V E+++  M  L AV+KETLR HPP   LL H ++   E+ GY+IPA   V
Sbjct:  274 KTRRVRKRWDDLLDKLIDDHAARMATHQDEDDDKDFI------YV--LLSLQK--EYGLTRDHIKAILIDMFEAGTDTSYMTLEFAMTELIRKPHLMKKLQEEVRRNVPAGQE-MVTEDNLPGMTDLKAVIKETLRLHPPVPLLLPHYSLDACEVAGYTIPANTRV 428          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71BM_ARATH (Cytochrome P450 71B22 OS=Arabidopsis thaliana GN=CYP71B22 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.453e-12
Identity = 43/137 (31.39%), Postives = 69/137 (50.36%), Query Frame = 2
Query:  185 ESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDG-LVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHVEFYT 592
            +S+  S I    +D +      G  Q+    +  + S+V  AG +  A T  WAM  L  +  + +KL +EI   +G +   + E+D+EK+ YL  V++ET R HPP   LL    + D ++ GY+IP    +E  T
Sbjct:  260 QSQDHSDIIGVMLDMINKESKVGSFQVTYDHLKGVMSDVFLAGVNAGAITMIWAMTELARHPRVMKKLQQEIREILGDNKEKITEQDLEKVHYLKLVIEETFRLHPPAPLLLPRETMSDLKIQGYNIPKNTMIEINT 396          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71BB_ARATH (Cytochrome P450 71B11 OS=Arabidopsis thaliana GN=CYP71B11 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.453e-12
Identity = 43/130 (33.08%), Postives = 67/130 (51.54%), Query Frame = 2
Query:  221 VDSLFGLEPTGREQLGEQEIVT-----LCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP--AGVHVEFY 589
            +D L  +E  G  +LGE ++       +   +++AG DTSA    W M +L+ N  + +K   E+   +     + EED+E++ YL  VVKET R  P    L+   A KD ++GGY IP    +HV  +
Sbjct:  264 IDLLLKME-RGEIELGEFQLTRDNTKGILFNILNAGIDTSAQVMTWVMTYLISNPRVMKKAQAEVREVIKNKDDIIEEDIERLEYLKMVVKETFRVLPLVPLLIPREASKDVKIGGYDIPKKTWIHVNIW 392          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71B5_ARATH (Cytochrome P450 71B5 OS=Arabidopsis thaliana GN=CYP71B5 PE=2 SV=1)

HSP 1 Score: 72.7886 bits (177), Expect = 1.453e-12
Identity = 42/132 (31.82%), Postives = 66/132 (50.00%), Query Frame = 2
Query:  200 SPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDG-LVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHVEFYT 592
            S I +  +D +      G  ++ +  +  L S+V  AG +  + T  W M  L  +  +  KL +EI AA+G +   + EED+EK+ YL  V++E  R HPP   LL    + D  + GYSIP    ++  T
Sbjct:  263 SDIISVMLDMINKPTEVGSYKVTDDHLKGLMSDVFLAGVNAGSITMIWTMTELSRHPRVMRKLQEEIRAALGPNKEKITEEDLEKVEYLKMVIEEAFRLHPPAPLLLPRLTMSDINIQGYSIPKNTMIQINT 394          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71D9_SOYBN (Cytochrome P450 71D9 OS=Glycine max GN=CYP71D9 PE=2 SV=1)

HSP 1 Score: 72.4034 bits (176), Expect = 1.897e-12
Identity = 36/102 (35.29%), Postives = 52/102 (50.98%), Query Frame = 2
Query:  263 LGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPA 568
            L ++ I  +  ++   G+DTS+ T  WAM  ++ N    EK+  E+     K+G       E + YL +VV ETLR HPP   LL     +  E+ GY IPA
Sbjct:  283 LSDESIKAVIWDIFGGGSDTSSATITWAMAEMIKNPRTMEKVQTEVRRVFDKEGRPNGSGTENLKYLKSVVSETLRLHPPAPLLLPRECGQACEINGYHIPA 384          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP17A_ONCMY (Steroid 17-alpha-hydroxylase/17,20 lyase OS=Oncorhynchus mykiss GN=cyp17a1 PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.478e-12
Identity = 30/106 (28.30%), Postives = 61/106 (57.55%), Query Frame = 2
Query:  263 LGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            L E  ++    ++  AG +T++T  +WA+ +L+ + ++Q+++ +E+ + VG D   +  D   +PYL+A ++E LR  P    L+ H A  DT +G +++  G  +
Sbjct:  294 LSEDHLLMTVGDIFGAGVETTSTVLKWAIAYLIHHPQVQQRIQEELDSVVGGDRTPQLSDRGSLPYLEATIREVLRIRPVAPLLIPHVAQTDTSIGKFTVRKGARI 399          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71BL_ARATH (Cytochrome P450 71B21 OS=Arabidopsis thaliana GN=CYP71B21 PE=2 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 2.478e-12
Identity = 42/137 (30.66%), Postives = 69/137 (50.36%), Query Frame = 2
Query:  185 ESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDG-LVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHVEFYT 592
            +SE  S I    +D +      G  ++    +  + S+V  AG +  A T  WA+  L  +  + +KL +EI   +G +   + E+D+EK+ YL  V++ET R HPP   LL    + D ++ GY+IP    +E  T
Sbjct:  260 QSEDHSDIVGVMLDMINKESKVGSFKVTYDHLKGVMSDVFLAGVNAGAITMIWALTELTRHPRVMKKLQQEIRELLGDNKEKITEQDLEKVHYLKLVIQETFRLHPPAPLLLPRETMSDVKIQGYNIPKNTMIEINT 396          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP27A_HUMAN (Sterol 26-hydroxylase, mitochondrial OS=Homo sapiens GN=CYP27A1 PE=1 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 3.237e-12
Identity = 48/149 (32.21%), Postives = 76/149 (51.01%), Query Frame = 2
Query:  146 RKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHP--PGHFLLSHAAIKDTELGGYSIPAGVHVEF 586
            +K   EK ++  ++ +   P G      L  L  +G  QL  +E +    E++ AG DT++ T  WA++HL  + EIQE L++E+V  V    + + +D   MP L AV+KETLR +P  P +   S    K+ E+ G+  P      F
Sbjct:  283 KKLIDEKLEDMEAQLQAAGPDGIQVSGYLHFLLASG--QLSPREAMGSLPELLMAGVDTTSNTLTWALYHLSKDPEIQEALHEEVVGVVPAGQVPQHKDFAHMPLLKAVLKETLRLYPVVPTN---SRIIEKEIEVDGFLFPKNTQFVF 426          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A4_CHICK (Cytochrome P450 1A4 OS=Gallus gallus GN=CYP1A4 PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 3.237e-12
Identity = 50/180 (27.78%), Postives = 83/180 (46.11%), Query Frame = 2
Query:   44 DFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGL---EPTG---REQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            DF+P+L  L  R M   K++  R    +  +++N  +  +K        E +  +     DSL G    + TG   R Q  ++ I+++ +++  AG DT  T   W M +  L   IQ+K+  E+   +G++   +  D   +PY +A + E  R      F + H   KDT L GY IP
Sbjct:  246 DFIPLLRYLPNRAMAAFKDVNARFSAFVQKIVQNHYSTFDK--------EHIRDV----TDSLIGHCQEKRTGEDVRVQPSDESIISIVNDLFGAGFDTVTTALSWCMMYAALYPHIQKKIQAELDQTIGRERRPRLSDRGMLPYTEAFILEAFRHSSLLPFTIPHCTTKDTVLNGYYIP 413          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A1_DICLA (Cytochrome P450 1A1 OS=Dicentrarchus labrax GN=cyp1a1 PE=2 SV=1)

HSP 1 Score: 71.633 bits (174), Expect = 3.237e-12
Identity = 45/174 (25.86%), Postives = 80/174 (45.98%), Query Frame = 2
Query:   44 DFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            DF+P+L  L    M+   ++  R  + +  ++       +K    +    ++       +D           Q+ +++IV + +++  AG DT +T   W++ +LV   EI+E+LY+E+   VG D      D   +P+L+A + E  R      F + H   KDT L GY IP
Sbjct:  241 DFIPVLQFLPSTTMKNFMDINARFNKFVQKIVSEHYTTYDKDNIRDITDSLIDHCEDRKLDE------NANVQMSDEKIVGIVNDLFGAGFDTISTALSWSVMYLVAYPEIEERLYQELKENVGLDRTPLLCDRPNLPFLEAFILEIFRHSSFLPFTIPHCTSKDTSLNGYFIP 408          
The following BLAST results are available for this feature:
BLAST of CX288495 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 193
Match NameE-valueIdentityDescription
C71C4_MAIZE1.453e-1232.53Cytochrome P450 71C4 OS=Zea mays GN=CYP71C4 PE=2 S... [more]
C71BM_ARATH1.453e-1231.39Cytochrome P450 71B22 OS=Arabidopsis thaliana GN=C... [more]
C71BB_ARATH1.453e-1233.08Cytochrome P450 71B11 OS=Arabidopsis thaliana GN=C... [more]
C71B5_ARATH1.453e-1231.82Cytochrome P450 71B5 OS=Arabidopsis thaliana GN=CY... [more]
C71D9_SOYBN1.897e-1235.29Cytochrome P450 71D9 OS=Glycine max GN=CYP71D9 PE=... [more]
CP17A_ONCMY2.478e-1228.30Steroid 17-alpha-hydroxylase/17,20 lyase OS=Oncorh... [more]
C71BL_ARATH2.478e-1230.66Cytochrome P450 71B21 OS=Arabidopsis thaliana GN=C... [more]
CP27A_HUMAN3.237e-1232.21Sterol 26-hydroxylase, mitochondrial OS=Homo sapie... [more]
CP1A4_CHICK3.237e-1227.78Cytochrome P450 1A4 OS=Gallus gallus GN=CYP1A4 PE=... [more]
CP1A1_DICLA3.237e-1225.86Cytochrome P450 1A1 OS=Dicentrarchus labrax GN=cyp... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionC02001A10SK IF1 Citrus clementina cDNA clone C02001A10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX288495 ID=CX288495; Name=CX288495; organism=Citrus clementina; type=EST; length=594bp
CATACTAAAAGATGTAATGCTGATAACATCACCACAGCTTCCGGATTTCT
TGCCGATTCTCACTCCATTATTTCGCAGGCAAATGAGAGAAGCAAAGGAG
TTGAGGAAGAGACAAATGGAGTGCTTGGTTCCACTGATAAGGAACAGAAA
AGCTTTTGTAGAGAAAGGCCAAAACCCTAACTCAGAGTCAGAGATGGTGA
GTCCAATTGGTGCTGCTTATGTGGATTCATTGTTTGGGCTGGAGCCAACG
GGGAGAGAGCAGCTGGGGGAACAAGAGATTGTGACATTGTGTTCTGAAGT
GATCAGTGCAGGCACTGACACTAGCGCAACAACGGCCGAATGGGCAATGC
ATCACTTGGTGCTAAACCAGGAGATTCAAGAAAAGTTGTATAAAGAAATT
GTAGCTGCTGTGGGGAAAGATGGGCTTGTTAAAGAAGAAGATGTCGAGAA
AATGCCTTATCTCGATGCTGTCGTGAAGGAAACTCTCCGGCGGCACCCTC
CTGGCCATTTTCTGCTGTCTCACGCTGCCATAAAGGATACTGAGCTTGGC
GGGTACTCCATCCCGGCAGGTGTTCACGTGGAATTCTACACTGC
back to top