CX288495

Overview
NameCX288495
Unique NameCX288495
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length594
Libraries
Library NameType
IF1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A2_BALAC (Cytochrome P450 1A2 OS=Balaenoptera acutorostrata GN=CYP1A2 PE=2 SV=3)

HSP 1 Score: 78.1814 bits (191), Expect = 3.458e-14
Identity = 50/177 (28.25%), Postives = 88/177 (49.72%), Query Frame = 2
Query:   44 DFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLF---GLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            DF PIL  L    +++ K   +R ++ L  +++      +K    N   ++V  +   Y D+     GL P       +++ V L +++ +AG D   T   W++ +LV N EIQ K+ +E+   +G+    +  D  ++PYL+A + ET R      F + H+ I+DT L G+ IP
Sbjct:  237 DFFPILKYLPNPALQKYKSFNRRFLQFLWKMVQEHHQDFDK----NRVQDIVGALFKHYEDNSRASGGLMP-------QKKTVNLVNDIFAAGFDPITTAISWSLLYLVTNPEIQRKIQQELDTVIGRARRPRLSDRSQLPYLEAFILETFRHSSFVPFTIPHSTIRDTTLNGFYIP 402          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71B4_ARATH (Cytochrome P450 71B4 OS=Arabidopsis thaliana GN=CYP71B4 PE=2 SV=2)

HSP 1 Score: 78.1814 bits (191), Expect = 3.458e-14
Identity = 41/93 (44.09%), Postives = 54/93 (58.06%), Query Frame = 2
Query:  293 SEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVG--KDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            S +  AG DTSA T  WAM  LV N  + +K   EI   +G  ++G + EED++K+ YL  VVKETLR HP    LL    + D ++ GY IP
Sbjct:  300 SNIYHAGIDTSAITLIWAMAELVRNPRVMKKAQDEIRTCIGIKQEGRIMEEDLDKLQYLKLVVKETLRLHPAAPLLLPRETMADIKIQGYDIP 392          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A2_MACFU (Cytochrome P450 1A2 OS=Macaca fuscata fuscata GN=CYP1A2 PE=2 SV=3)

HSP 1 Score: 77.7962 bits (190), Expect = 4.516e-14
Identity = 52/174 (29.89%), Postives = 85/174 (48.85%), Query Frame = 2
Query:   44 DFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            DF PIL  L    ++  K   +R    L   ++      +K    NS  ++    GA +  S  G   +G   + +++IV L +++  A  DT AT   W++ +LV   EIQ K+ KE+ A +G+    +  D  ++PYL+A + ET R      F + H+  +DT L G+ IP
Sbjct:  237 DFFPILRYLPNPALQRFKAFNQRFRRFLQKTVQEHYQDFDK----NSVQDIT---GALFKHSKKGPRASGN-LIPQEKIVNLVNDIFGAEFDTIATAISWSLMYLVTKPEIQRKIQKELDAVIGRGRRPRLSDRPQLPYLEAFILETFRHSSFVPFTIPHSTTRDTTLNGFYIP 402          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71AC_ARATH (Cytochrome P450 71A12 OS=Arabidopsis thaliana GN=CYP71A12 PE=2 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 4.516e-14
Identity = 46/126 (36.51%), Postives = 68/126 (53.97%), Query Frame = 2
Query:  218 YVDSLFGLEPTGREQLGEQ----EIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDG-LVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            +VD L  +E    + +G Q    +I  +  ++   GT TS+T  EW M  L+ N  + +KL  EI + +   G  +KE+DVE M YL AV+KE  R HPP   +L     +D ++ GY+I AG  V
Sbjct:  266 FVDILLSIE--SEKSIGFQAQRDDIKFMILDMFIGGTSTSSTLLEWIMTELIRNPNVMKKLQDEIRSTIRPHGSYIKEKDVENMKYLKAVIKEVFRVHPPLPLILPRLLSEDVKVKGYNIAAGTEV 389          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A1_SPAAU (Cytochrome P450 1A1 OS=Sparus aurata GN=cyp1a1 PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 5.898e-14
Identity = 48/182 (26.37%), Postives = 85/182 (46.70%), Query Frame = 2
Query:   20 LITSPQLPDFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            ++ S    DF+PIL  L    M++   +  R    +  ++       +K    +    ++       +D           Q+ ++++V + +++  AG DT +T   W++ +LV   EIQE+LY+E+  +VG D      D  K+P+L+A + E  R      F + H + KDT L GY IP
Sbjct:  232 VVASGNPADFIPILQYLPSTSMKKFVSINDRFNAFVQKIVSEHYTTFDKDNIRDITDSLIDHCEDRKLDE------NSNVQMSDEKVVGIVNDLFGAGFDTISTALSWSVMYLVAYPEIQERLYQEMKESVGLDRTPCLSDKPKLPFLEAFILEIFRHSSFLPFTIPHCSSKDTSLNGYFIP 407          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A1_SHEEP (Cytochrome P450 1A1 OS=Ovis aries GN=CYP1A1 PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 5.898e-14
Identity = 44/176 (25.00%), Postives = 85/176 (48.30%), Query Frame = 2
Query:   44 DFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAG 571
            DF+P+L  L    +   K+L +R    +  +++      EKG   +    ++       +D    +      QL +++I+ +  ++  AG DT  T   W++ +LV +  +Q+K+ +E+   +G+    +  D  ++PYL+A + ET R      F + H+  +DT L G+ IP G
Sbjct:  242 DFIPVLRYLPNTALDLFKDLNRRFYVFVQKIVKEHYKTFEKGHIRDITDSLIEHCQDKRLDENANI------QLSDEKIINVVMDLFGAGFDTVTTAISWSLLYLVTSPRVQKKIQEELDTVIGRARWPQLSDRPQLPYLEAFILETFRHSSFVPFTIPHSTTRDTNLNGFYIPKG 411          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71BV_ARATH (Cytochrome P450 71B34 OS=Arabidopsis thaliana GN=CYP71B34 PE=2 SV=1)

HSP 1 Score: 77.411 bits (189), Expect = 5.898e-14
Identity = 42/120 (35.00%), Postives = 63/120 (52.50%), Query Frame = 2
Query:  218 YVDSLFGLEPT----GREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            +VD L  LE      G ++L    I  +  +V+ AG DTSA T  WAM  L  N  + +K+  EI + +     +  +D +K+ YL  V+KET R HP    L+   A+ + E+ GY+IP
Sbjct:  267 FVDLLLRLEKEEAVLGNDKLTRNHIKAILMDVLLAGMDTSAITMTWAMAELAKNPRVMKKVQSEIRSQIKNKERISFDDTDKLEYLKMVIKETWRLHPTTPLLIPREAMSEFEINGYTIP 386          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C84A1_ARATH (Cytochrome P450 84A1 OS=Arabidopsis thaliana GN=CYP84A1 PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 7.704e-14
Identity = 48/147 (32.65%), Postives = 78/147 (53.06%), Query Frame = 2
Query:  158 VEKGQNPNS-------ESEMVSPIGAAYVDSLFGLEPTGREQ----LGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            ++K +N N+       +++MV  + A Y +    +  T   Q    L    I  +  +V+  GT+T A+  EWA+  L+ + E  +++ +E+   VG D  V+E D+EK+ YL   +KETLR HPP   LL H   +DT + G+ IP
Sbjct:  258 MKKKENQNAVDDGDVVDTDMVDDLLAFYSEEAKLVSETADLQNSIKLTRDNIKAIIMDVMFGGTETVASAIEWALTELLRSPEDLKRVQQELAEVVGLDRRVEESDIEKLTYLKCTLKETLRMHPPIPLLL-HETAEDTSIDGFFIP 403          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C82A1_PEA (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 77.0258 bits (188), Expect = 7.704e-14
Identity = 38/95 (40.00%), Postives = 56/95 (58.95%), Query Frame = 2
Query:  296 EVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            E+I  G+DT+A T  WAM  L+ +  + EKL +E+   +GK+  V E D+ K+ YL A++KETLR +PP  F       +D  +GGY I  G  +
Sbjct:  336 ELILGGSDTTAGTLTWAMCLLLKHPHVLEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTIGGYHIKKGTRL 430          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C75AK_ARATH (Cytochrome P450 705A20 OS=Arabidopsis thaliana GN=CYP705A20 PE=1 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 7.704e-14
Identity = 41/114 (35.96%), Postives = 66/114 (57.89%), Query Frame = 2
Query:  161 EKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPP 502
            EK  N +  + ++  +  AY D           ++    I +L  E++  GTDTSA T +W M  L+ N+ + ++L +EI + VG+  L++E+D+ K+PYL +VVKE LR HPP
Sbjct:  265 EKKPNEHQGTYLMDVLLEAYEDE------KAEHKITRNHIKSLFVELLLGGTDTSAQTIQWTMAELINNRNVLKRLREEIDSVVGETRLIQEKDLPKLPYLQSVVKEGLRLHPP 372          
The following BLAST results are available for this feature:
BLAST of CX288495 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 193
Match NameE-valueIdentityDescription
CP1A2_BALAC3.458e-1428.25Cytochrome P450 1A2 OS=Balaenoptera acutorostrata ... [more]
C71B4_ARATH3.458e-1444.09Cytochrome P450 71B4 OS=Arabidopsis thaliana GN=CY... [more]
CP1A2_MACFU4.516e-1429.89Cytochrome P450 1A2 OS=Macaca fuscata fuscata GN=C... [more]
C71AC_ARATH4.516e-1436.51Cytochrome P450 71A12 OS=Arabidopsis thaliana GN=C... [more]
CP1A1_SPAAU5.898e-1426.37Cytochrome P450 1A1 OS=Sparus aurata GN=cyp1a1 PE=... [more]
CP1A1_SHEEP5.898e-1425.00Cytochrome P450 1A1 OS=Ovis aries GN=CYP1A1 PE=2 S... [more]
C71BV_ARATH5.898e-1435.00Cytochrome P450 71B34 OS=Arabidopsis thaliana GN=C... [more]
C84A1_ARATH7.704e-1432.65Cytochrome P450 84A1 OS=Arabidopsis thaliana GN=CY... [more]
C82A1_PEA7.704e-1440.00Cytochrome P450 82A1 (Fragment) OS=Pisum sativum G... [more]
C75AK_ARATH7.704e-1435.96Cytochrome P450 705A20 OS=Arabidopsis thaliana GN=... [more]

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Properties
Property NameValue
Genbank descriptionC02001A10SK IF1 Citrus clementina cDNA clone C02001A10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX288495 ID=CX288495; Name=CX288495; organism=Citrus clementina; type=EST; length=594bp
CATACTAAAAGATGTAATGCTGATAACATCACCACAGCTTCCGGATTTCT
TGCCGATTCTCACTCCATTATTTCGCAGGCAAATGAGAGAAGCAAAGGAG
TTGAGGAAGAGACAAATGGAGTGCTTGGTTCCACTGATAAGGAACAGAAA
AGCTTTTGTAGAGAAAGGCCAAAACCCTAACTCAGAGTCAGAGATGGTGA
GTCCAATTGGTGCTGCTTATGTGGATTCATTGTTTGGGCTGGAGCCAACG
GGGAGAGAGCAGCTGGGGGAACAAGAGATTGTGACATTGTGTTCTGAAGT
GATCAGTGCAGGCACTGACACTAGCGCAACAACGGCCGAATGGGCAATGC
ATCACTTGGTGCTAAACCAGGAGATTCAAGAAAAGTTGTATAAAGAAATT
GTAGCTGCTGTGGGGAAAGATGGGCTTGTTAAAGAAGAAGATGTCGAGAA
AATGCCTTATCTCGATGCTGTCGTGAAGGAAACTCTCCGGCGGCACCCTC
CTGGCCATTTTCTGCTGTCTCACGCTGCCATAAAGGATACTGAGCTTGGC
GGGTACTCCATCCCGGCAGGTGTTCACGTGGAATTCTACACTGC
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