CX288495

Overview
NameCX288495
Unique NameCX288495
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length594
Libraries
Library NameType
IF1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C5133_DICDI (Probable cytochrome P450 513A3 OS=Dictyostelium discoideum GN=cyp513a3 PE=3 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 7.704e-14
Identity = 56/193 (29.02%), Postives = 86/193 (44.56%), Query Frame = 2
Query:   32 PQLPDFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVE----------KMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            P L DF+PI            KEL     + +    ++R   +E G     E E +       +  L  L+      +  + +   C ++ISAGTDTSA T   A+  L+ NQEIQ K +  I ++   D    ++D E           +PY+  ++KET R+HP     L H   +D E+ GY I AG ++
Sbjct:  210 PILSDFIPIPLKKSGVFFDSYKELEIETDKLIEKFKKSRNEKIENGTYNEEEDESI-------LSKL--LKEYEHGNITWECVSHTCIDIISAGTDTSANTLVMALIELINNQEIQSKAFSSIRSSCLNDSNDDDDDDEIVITHSKYRSLLPYISMIIKETFRKHPIALLGLPHVTTEDVEIDGYKIEAGTYI 393          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: KO1_ARATH (Ent-kaurene oxidase OS=Arabidopsis thaliana GN=KO1 PE=1 SV=2)

HSP 1 Score: 76.6406 bits (187), Expect = 1.006e-13
Identity = 56/180 (31.11%), Postives = 91/180 (50.56%), Query Frame = 2
Query:   44 DFLPILTPLFRRQMR-EAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            DF P L  +  +      ++  KR++  +  LI++R       QN  SES+        Y++ L     T    L +++I  L  E I    DT+  T EWA++ L  +  +Q++L KEI    G +   KEE + ++PYL+ V  ETLR++ P   +    A +DT++GGY +PAG  +
Sbjct:  242 DFFPYLKWIPNKSFEARIQQKHKRRLAVMNALIQDRLK-----QN-GSESD-----DDCYLNFLMSEAKT----LTKEQIAILVWETIIETADTTLVTTEWAIYELAKHPSVQDRLCKEIQNVCGGEKF-KEEQLSQVPYLNGVFHETLRKYSPAPLVPIRYAHEDTQIGGYHVPAGSEI 405          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A2_PONAB (Cytochrome P450 1A2 OS=Pongo abelii GN=CYP1A2 PE=2 SV=3)

HSP 1 Score: 76.6406 bits (187), Expect = 1.006e-13
Identity = 53/174 (30.46%), Postives = 85/174 (48.85%), Query Frame = 2
Query:   44 DFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            DF PIL  L    ++  K   +R +  L      RK   E  Q+ +  S +   +GA +  S  G   +G   + +++IV L +++  AG DT  T   W++ +LV   EIQ K+ KE+   +G+    +  D  ++PYL A + ET R      F + H+  +DT L G+ IP
Sbjct:  237 DFFPILRYLPNPALQRFKAFNQRFLRFL------RKTVQEHYQDFDKNS-VQDIMGALFKYSKKGPRASGN-LIPQEKIVNLVNDIFGAGFDTVTTAISWSLMYLVTKPEIQRKIQKELDTMIGRGRRPRLSDRPQLPYLKAFILETFRHSSFLPFTIPHSTTRDTTLNGFYIP 402          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A1_LIZAU (Cytochrome P450 1A1 OS=Liza aurata GN=cyp1a1 PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.006e-13
Identity = 47/174 (27.01%), Postives = 80/174 (45.98%), Query Frame = 2
Query:   44 DFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            DF+PIL  L  + M++   +  R +  +  ++    A   K    +    ++       +D           Q+ ++++V + +++  AG DT +T   W++ +LV   EIQE+LY+E+   VG D      D   +P L+A + E  R      F + H   KDT L GY IP
Sbjct:  240 DFIPILQYLPNKTMKKFVNINDRFISFVQKIVSEHYATFNKDNIRDITDSLIDHCEDRKLDE------NANVQMSDEKVVGIVNDLFGAGLDTISTALSWSVMYLVAYPEIQERLYQELKENVGLDRTPVLSDRNNLPLLEAFILEIFRHSSFLPFTIPHCTTKDTSLNGYYIP 407          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C82A3_SOYBN (Cytochrome P450 82A3 OS=Glycine max GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.006e-13
Identity = 42/111 (37.84%), Postives = 61/111 (54.95%), Query Frame = 2
Query:  260 QLGEQEIVTLCS----EVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            Q+G  +  T+C     E+I  GTD++A T  WA+  L+ N     K  +EI   +GKD  ++E D+ K+ YL A+VKETLR +PP  F       ++  LGGY I  G  +
Sbjct:  303 QIGAFDADTICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDEYIRESDISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRL 413          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71AO_ARATH (Cytochrome P450 71A24 OS=Arabidopsis thaliana GN=CYP71A24 PE=2 SV=3)

HSP 1 Score: 76.6406 bits (187), Expect = 1.006e-13
Identity = 39/107 (36.45%), Postives = 58/107 (54.21%), Query Frame = 2
Query:  260 QLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            ++    I  +  +V     DT+ T  EWAM  L+ + E  ++L +E+         V E+D++ M YL AV+KETLR HPP   ++ H +  D +L  Y IPAG HV
Sbjct:  277 EIDRLSIKAIILDVFVGDMDTTYTLLEWAMTELLCHHECLDRLQEEVRMVCKDKSGVSEDDLQDMKYLKAVIKETLRLHPPLPLMVPHESTHDVKLRDYHIPAGTHV 383          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71AJ_ARATH (Cytochrome P450 71A19 OS=Arabidopsis thaliana GN=CYP71A19 PE=2 SV=1)

HSP 1 Score: 76.6406 bits (187), Expect = 1.006e-13
Identity = 54/181 (29.83%), Postives = 93/181 (51.38%), Query Frame = 2
Query:   44 DFLPILTPL--FRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            +++P L+ +   R Q  + +E+ KR  E L  +++  +       N ++ S++V        D+L  +      Q  +  +  +  ++  AGT TS +  EWAM  L+ N ++ +KL +EI ++  +   V E++ EKM YL AV+KE LR  PP   ++     +D  L GY+IPAG  V
Sbjct:  222 EYIPSLSWIDKIRGQDHKMEEVDKRFDEFLERVVKEHE-----DANKDTRSDLV--------DTLLTI------QSDKSALKLIIWDMFLAGTATSLSFLEWAMTELMRNPKVMKKLQEEIRSSSRQGLFVTEKEAEKMDYLQAVIKEALRLRPPAPLMVPRVFSEDVTLKGYNIPAGTQV 383          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A2_CALJA (Cytochrome P450 1A2 OS=Callithrix jacchus GN=CYP1A2 PE=2 SV=3)

HSP 1 Score: 76.2554 bits (186), Expect = 1.314e-13
Identity = 52/180 (28.89%), Postives = 83/180 (46.11%), Query Frame = 2
Query:   26 TSPQLPDFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            TS    DF PIL  L    ++  K   +R +  L      R+   E  Q+  S+   V  I  A             + + +++IV L +++  AG DT  T   W++ +LV   EIQ K+ KE+   +G+    +  D  ++PYL+A + ET R      F + H+  +DT L G+ IP
Sbjct:  231 TSGNPVDFFPILRYLPNPALQRFKAFNQRFLRFL------RETVQEHYQD--SDKNSVQDITGALFKHCEKRSGASGDLIPQEKIVNLVNDIFGAGFDTITTAISWSLMYLVTKPEIQRKIQKELDTVIGRGRRPRLSDRPQLPYLEAFILETFRHSSFVPFTIPHSTTRDTTLKGFYIP 402          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP27A_RAT (Sterol 26-hydroxylase, mitochondrial OS=Rattus norvegicus GN=Cyp27a1 PE=1 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.716e-13
Identity = 42/104 (40.38%), Postives = 58/104 (55.77%), Query Frame = 2
Query:  254 REQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            +E L  QE V    E+I AG DT++ T  WA++HL  N EIQE L+KE+   V    + + +D   MP L AV+KETLR +P           K+TE+ G+  P
Sbjct:  319 KELLSPQETVGTFPELILAGVDTTSNTLTWALYHLSKNPEIQEALHKEVTGVVPFGKVPQNKDFAHMPLLKAVIKETLRLYPVVPTNSRIITEKETEINGFLFP 422          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A1_FELCA (Cytochrome P450 1A1 OS=Felis catus GN=CYP1A1 PE=2 SV=1)

HSP 1 Score: 75.8702 bits (185), Expect = 1.716e-13
Identity = 45/174 (25.86%), Postives = 83/174 (47.70%), Query Frame = 2
Query:   44 DFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            DF PIL  L    +   K++ ++    +  +++      EKG   +    ++       +D    +      QL +++IV + S++  AG DT  T   W + +LV +  +QEK+ KE+   +G++   +  D  ++PY++A + E  R      F + H+  KDT L G+ IP
Sbjct:  240 DFFPILRYLPNPALDFFKDVNEKFSIFIHKMVKEHYKTFEKGHIRDITDSLIEHCQDKRLDENANI------QLSDEKIVNVVSDLFGAGFDTVTTAISWCLMYLVTSPNVQEKIQKELDTVIGRERQPRLSDRLQLPYMEAFILEMFRHTSFVPFTIPHSTTKDTSLSGFYIP 407          
The following BLAST results are available for this feature:
BLAST of CX288495 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 193
Match NameE-valueIdentityDescription
C5133_DICDI7.704e-1429.02Probable cytochrome P450 513A3 OS=Dictyostelium di... [more]
KO1_ARATH1.006e-1331.11Ent-kaurene oxidase OS=Arabidopsis thaliana GN=KO1... [more]
CP1A2_PONAB1.006e-1330.46Cytochrome P450 1A2 OS=Pongo abelii GN=CYP1A2 PE=2... [more]
CP1A1_LIZAU1.006e-1327.01Cytochrome P450 1A1 OS=Liza aurata GN=cyp1a1 PE=2 ... [more]
C82A3_SOYBN1.006e-1337.84Cytochrome P450 82A3 OS=Glycine max GN=CYP82A3 PE=... [more]
C71AO_ARATH1.006e-1336.45Cytochrome P450 71A24 OS=Arabidopsis thaliana GN=C... [more]
C71AJ_ARATH1.006e-1329.83Cytochrome P450 71A19 OS=Arabidopsis thaliana GN=C... [more]
CP1A2_CALJA1.314e-1328.89Cytochrome P450 1A2 OS=Callithrix jacchus GN=CYP1A... [more]
CP27A_RAT1.716e-1340.38Sterol 26-hydroxylase, mitochondrial OS=Rattus nor... [more]
CP1A1_FELCA1.716e-1325.86Cytochrome P450 1A1 OS=Felis catus GN=CYP1A1 PE=2 ... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionC02001A10SK IF1 Citrus clementina cDNA clone C02001A10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX288495 ID=CX288495; Name=CX288495; organism=Citrus clementina; type=EST; length=594bp
CATACTAAAAGATGTAATGCTGATAACATCACCACAGCTTCCGGATTTCT
TGCCGATTCTCACTCCATTATTTCGCAGGCAAATGAGAGAAGCAAAGGAG
TTGAGGAAGAGACAAATGGAGTGCTTGGTTCCACTGATAAGGAACAGAAA
AGCTTTTGTAGAGAAAGGCCAAAACCCTAACTCAGAGTCAGAGATGGTGA
GTCCAATTGGTGCTGCTTATGTGGATTCATTGTTTGGGCTGGAGCCAACG
GGGAGAGAGCAGCTGGGGGAACAAGAGATTGTGACATTGTGTTCTGAAGT
GATCAGTGCAGGCACTGACACTAGCGCAACAACGGCCGAATGGGCAATGC
ATCACTTGGTGCTAAACCAGGAGATTCAAGAAAAGTTGTATAAAGAAATT
GTAGCTGCTGTGGGGAAAGATGGGCTTGTTAAAGAAGAAGATGTCGAGAA
AATGCCTTATCTCGATGCTGTCGTGAAGGAAACTCTCCGGCGGCACCCTC
CTGGCCATTTTCTGCTGTCTCACGCTGCCATAAAGGATACTGAGCTTGGC
GGGTACTCCATCCCGGCAGGTGTTCACGTGGAATTCTACACTGC
back to top