CX288495

Overview
NameCX288495
Unique NameCX288495
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length594
Libraries
Library NameType
IF1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71BQ_ARATH (Cytochrome P450 71B26 OS=Arabidopsis thaliana GN=CYP71B26 PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 2.241e-13
Identity = 49/147 (33.33%), Postives = 70/147 (47.62%), Query Frame = 2
Query:  140 RNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEP----TGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPA 568
            R+  AF E+  + + E + V       VD L  LE      G  +L    I  +   ++  G DTSA T  WAM  L  N  + +K+  EI   +     +  +D +K+ YL  V+KET R HPP   LL    I + E+ GY+IPA
Sbjct:  243 RDLDAFYEQIFDLHKEEKEVG--SEDLVDVLLRLEKEEIVVGNGKLTRNHIKAILMNILLGGIDTSAITMTWAMAELAKNPRVMKKVQAEIRNQIKNKERISFDDTDKLEYLKMVIKETWRLHPPTPLLLPRDVITEFEINGYTIPA 387          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71BC_ARATH (Cytochrome P450 71B12 OS=Arabidopsis thaliana GN=CYP71B12 PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 2.241e-13
Identity = 47/159 (29.56%), Postives = 80/159 (50.31%), Query Frame = 2
Query:  134 LIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVT-----LCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP--AGVHVEFY 589
            L +   AF ++    + E E++       +D L  +E  G   LGE ++       + + +++AG DTSA    W M +L+ N  + +K   E+   +     + EED+E++ YL  V+KET R +P    L+   A KD ++GGY+IP    +HV  +
Sbjct:  238 LFKAMDAFFDQSIKHHLEDEIIKD---DIIDLLLKME-RGETTLGEFQLTRDHTKGILANILNAGIDTSAQVMTWVMTYLISNPRVLKKAQAEVREVIKHKDDIIEEDIERLQYLKMVIKETFRINPLVPLLIPREASKDVKIGGYNIPKKTWIHVNIW 392          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71AG_ARATH (Cytochrome P450 71A16 OS=Arabidopsis thaliana GN=CYP71A16 PE=2 SV=1)

HSP 1 Score: 75.485 bits (184), Expect = 2.241e-13
Identity = 44/123 (35.77%), Postives = 65/123 (52.85%), Query Frame = 2
Query:  218 YVDSLFGLE--PTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            +VD L  ++   T + QL + ++  +  E+    T T++   EWAM  L+ N E  +KL  EI +    +  V  ++VE M YL AV+KE LR HPP   L+     +D +L GY I AG  V
Sbjct:  267 FVDMLLTIQRDETAQCQLDKSDLKVIIFEMFLGSTTTTSAVIEWAMTRLMRNPECLKKLQDEIRSVSKMNSYVSGKEVENMNYLKAVIKEVLRLHPPLPLLVPRLLSEDVKLKGYDITAGTQV 389          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A1_OPSTA (Cytochrome P450 1A1 OS=Opsanus tau GN=cyp1a1 PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 2.927e-13
Identity = 48/182 (26.37%), Postives = 87/182 (47.80%), Query Frame = 2
Query:   20 LITSPQLPDFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            ++ +  L DF+P+L  L    M++   +  R  + +  ++    A   K    +    ++       +D    +      Q+ +++IV + +++  AG DT +T   W++ +LV   EIQE+LY+EI  +VG + +    D   +P+LDA + E  R      F + H   K+T L GY IP
Sbjct:  232 VVGTGNLADFIPVLRFLPSTAMKKFLSINDRFDKFVKKIVSEHYATYNKDNIRDITDSLIDHCEDRKLDENCNV------QVSDEKIVGIVNDLFGAGFDTVSTGLSWSVMYLVAYPEIQERLYQEIKDSVGTERMPLLSDRPSLPFLDAFILEIFRHSSFLPFTIPHCTTKNTSLNGYFIP 407          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71BX_ARATH (Cytochrome P450 71B36 OS=Arabidopsis thaliana GN=CYP71B36 PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 2.927e-13
Identity = 41/120 (34.17%), Postives = 61/120 (50.83%), Query Frame = 2
Query:  218 YVDSLFGLEPT----GREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            +VD L  LE      G  +L    I  +   V+  G  TSA T  WAM  L+ N  + +K+  EI   +GK  ++  +D++++ YL  V+ ET R HPP  FL+    + + EL  Y IP
Sbjct:  267 FVDLLLRLEKEETVIGYGKLTRNHIKAILMNVLIGGIGTSAITMTWAMTELMRNPRVMKKVQSEIRNQIGKKSMITLDDIDQLHYLKMVINETWRLHPPSPFLIPRQVMSEFELNDYVIP 386          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71B1_THLAR (Cytochrome P450 71B1 OS=Thlaspi arvense GN=CYP71B1 PE=2 SV=1)

HSP 1 Score: 75.0998 bits (183), Expect = 2.927e-13
Identity = 44/130 (33.85%), Postives = 67/130 (51.54%), Query Frame = 2
Query:  221 VDSLFGLEPTGREQLGEQEIVT-----LCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP--AGVHVEFY 589
            +D L  +E  G   LGE E+       +   +++AG DTSA T  WAM HL+ N  + +KL  EI   +     + ++DVE++ Y   V+KET R  P    L+   A KD ++ GY +P    +HV  +
Sbjct:  264 IDLLLKME-RGEGSLGEYELTREHTKGILMNILTAGIDTSAQTMTWAMTHLLANPRVMKKLQAEIREKIKNIDEITDDDVEQLDYFKLVLKETFRISPIVPVLVPRVAAKDLKIAGYDVPEKTWIHVNMW 392          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A1_RABIT (Cytochrome P450 1A1 OS=Oryctolagus cuniculus GN=CYP1A1 PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 3.823e-13
Identity = 37/111 (33.33%), Postives = 61/111 (54.95%), Query Frame = 2
Query:  239 LEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAG 571
            L+     Q+ +++ V +  ++  AG DT  T   W++ +LV    IQ K+ +E+ A VG+    +  D  ++PYL+AV+ ET R      F + H+  +DT LGG+ IP G
Sbjct:  298 LDENANVQVSDEKTVGIVLDLFGAGFDTVTTAISWSLMYLVTKPRIQRKIQEELDAVVGRARRPRFSDRPQLPYLEAVIMETFRHTSFLPFTIPHSTTRDTSLGGFYIPKG 408          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A1_ONCMY (Cytochrome P450 1A1 OS=Oncorhynchus mykiss GN=cyp1a1 PE=2 SV=2)

HSP 1 Score: 74.7146 bits (182), Expect = 3.823e-13
Identity = 54/179 (30.17%), Postives = 86/179 (48.04%), Query Frame = 2
Query:   44 DFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSES---EMVSPIGAAYVDSLFG--LEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            DF+PIL  L  R M+   ++  R              FV+K  + + ES   + +  I  + +D      L+     Q+ +++IV + +++  AG DT +T   WA+ +LV   EIQE+L++E+   VG     +  D   +P L+A + E  R      F + H  IKDT L GY IP
Sbjct:  240 DFIPILRYLPNRTMKRFMDINDRFNN-----------FVQKIVSEHYESYDKDNIRDITDSLIDHCEDRKLDENANIQVSDEKIVGIVNDLFGAGFDTISTALSWAVVYLVAYPEIQERLHQELKEKVGMIRTPRLSDKINLPLLEAFILEIFRHSSFLPFTIPHCTIKDTSLNGYFIP 407          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C75A5_EUSGR (Flavonoid 3',5'-hydroxylase OS=Eustoma grandiflorum GN=CYP75A5 PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 3.823e-13
Identity = 38/108 (35.19%), Postives = 59/108 (54.63%), Query Frame = 2
Query:  257 EQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            E+L    I  L   + +AGTDTS++  EWA+  L+ N  I ++  +E+   +G+D    E D+ K+PYL A+ KE  R+HP     L   A +  E+ G+ IP G  +
Sbjct:  288 ERLHTVNIKALLLNMFTAGTDTSSSVIEWALAELLKNPIILKRAQEEMDGVIGRDRRFLEADISKLPYLQAICKEAFRKHPSTPLNLPRIASQACEVNGHYIPKGTRL 395          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71BO_ARATH (Cytochrome P450 71B24 OS=Arabidopsis thaliana GN=CYP71B24 PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 3.823e-13
Identity = 38/96 (39.58%), Postives = 57/96 (59.38%), Query Frame = 2
Query:  287 LCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVG--KDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPA 568
            +  ++  AG DTSA T  WAM  LV N  + +K+  EI   +G  ++  ++E+DV+K+ YL  VVKETLR HP    LL    +   ++ GY+IP+
Sbjct:  294 IIQDIYLAGVDTSAITMIWAMAELVKNPRVMKKVQDEIRTCIGIKQNEKIEEDDVDKLQYLKLVVKETLRLHPAAPLLLPRETMSQIKIQGYNIPS 389          
The following BLAST results are available for this feature:
BLAST of CX288495 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 193
Match NameE-valueIdentityDescription
C71BQ_ARATH2.241e-1333.33Cytochrome P450 71B26 OS=Arabidopsis thaliana GN=C... [more]
C71BC_ARATH2.241e-1329.56Cytochrome P450 71B12 OS=Arabidopsis thaliana GN=C... [more]
C71AG_ARATH2.241e-1335.77Cytochrome P450 71A16 OS=Arabidopsis thaliana GN=C... [more]
CP1A1_OPSTA2.927e-1326.37Cytochrome P450 1A1 OS=Opsanus tau GN=cyp1a1 PE=2 ... [more]
C71BX_ARATH2.927e-1334.17Cytochrome P450 71B36 OS=Arabidopsis thaliana GN=C... [more]
C71B1_THLAR2.927e-1333.85Cytochrome P450 71B1 OS=Thlaspi arvense GN=CYP71B1... [more]
CP1A1_RABIT3.823e-1333.33Cytochrome P450 1A1 OS=Oryctolagus cuniculus GN=CY... [more]
CP1A1_ONCMY3.823e-1330.17Cytochrome P450 1A1 OS=Oncorhynchus mykiss GN=cyp1... [more]
C75A5_EUSGR3.823e-1335.19Flavonoid 3',5'-hydroxylase OS=Eustoma grandifloru... [more]
C71BO_ARATH3.823e-1339.58Cytochrome P450 71B24 OS=Arabidopsis thaliana GN=C... [more]

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Properties
Property NameValue
Genbank descriptionC02001A10SK IF1 Citrus clementina cDNA clone C02001A10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX288495 ID=CX288495; Name=CX288495; organism=Citrus clementina; type=EST; length=594bp
CATACTAAAAGATGTAATGCTGATAACATCACCACAGCTTCCGGATTTCT
TGCCGATTCTCACTCCATTATTTCGCAGGCAAATGAGAGAAGCAAAGGAG
TTGAGGAAGAGACAAATGGAGTGCTTGGTTCCACTGATAAGGAACAGAAA
AGCTTTTGTAGAGAAAGGCCAAAACCCTAACTCAGAGTCAGAGATGGTGA
GTCCAATTGGTGCTGCTTATGTGGATTCATTGTTTGGGCTGGAGCCAACG
GGGAGAGAGCAGCTGGGGGAACAAGAGATTGTGACATTGTGTTCTGAAGT
GATCAGTGCAGGCACTGACACTAGCGCAACAACGGCCGAATGGGCAATGC
ATCACTTGGTGCTAAACCAGGAGATTCAAGAAAAGTTGTATAAAGAAATT
GTAGCTGCTGTGGGGAAAGATGGGCTTGTTAAAGAAGAAGATGTCGAGAA
AATGCCTTATCTCGATGCTGTCGTGAAGGAAACTCTCCGGCGGCACCCTC
CTGGCCATTTTCTGCTGTCTCACGCTGCCATAAAGGATACTGAGCTTGGC
GGGTACTCCATCCCGGCAGGTGTTCACGTGGAATTCTACACTGC
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