CX288495

Overview
NameCX288495
Unique NameCX288495
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length594
Libraries
Library NameType
IF1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C13B1_CAEEL (Putative cytochrome P450 cyp-13B1 OS=Caenorhabditis elegans GN=cyp-13B1 PE=2 SV=2)

HSP 1 Score: 70.0922 bits (170), Expect = 9.417e-12
Identity = 53/208 (25.48%), Postives = 100/208 (48.08%), Query Frame = 2
Query:    2 ILKDVMLITSPQLPDFLPILTPLFRRQMR---------EAKELRKRQMECLVPL---IRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLE--PTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHVE 583
            I+K + + T   LP +  +L P F   ++         +  ++ K  + C   +   I+ R+   + G   N+E++ +      Y + +  +E   T  +++  ++++  C   + AG DT+A +  +A + L  + E  +   +E+   VG +  V  +D+ K+ YLDAVV+E+LR +P   F  S   +K T LG   I  GV +E
Sbjct:  205 IVKSIFMRTHRVLPWYFTVLFPQFEHLVKRMFYNHAAVQGGDIEKLLLICKKTVESRIQEREENAKLGFE-NAENDFIDMFLNYYSEQVEDIEFGSTVEKKVTAEDVIGACFVFLLAGFDTTANSLAYASYLLAKHPEKMKLAQEEVDTVVGSEN-VSYDDMTKLKYLDAVVRESLRLYPVAWFACSRECVKPTTLGDIYIDKGVKIE 410          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP303_DROME (Probable cytochrome P450 303a1 OS=Drosophila melanogaster GN=Cyp303a1 PE=1 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.606e-11
Identity = 38/119 (31.93%), Postives = 65/119 (54.62%), Query Frame = 2
Query:  221 VDSLFGLEPTGREQLG---EQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVK-EEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            +DS    +  G ++ G   +Q ++ +C ++  AG++T+  +  +   HLVL  EIQE+ ++EI   VG + + +   D  K+PY +A+  E +R      F + H A+ DT L GY IP
Sbjct:  278 MDSYLRAQDEGNDEKGMFSDQSLLAICLDMFLAGSETTNKSLGFCFMHLVLQPEIQERAFQEIKEVVGLERIPEWSRDRTKLPYCEAITLEAVRMFMLHTFGIPHRAVCDTRLSGYEIP 396          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP17A_CHICK (Steroid 17-alpha-hydroxylase/17,20 lyase OS=Gallus gallus GN=CYP17A1 PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.606e-11
Identity = 33/107 (30.84%), Postives = 58/107 (54.21%), Query Frame = 2
Query:  260 QLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            +L +  ++    ++  AG +T+ T  +WA+ +L+   E+Q+K+ +E+   +G        D   +PYL+A + E LR  P    L+ H ++ DT +G YSIP G  V
Sbjct:  289 ELTDDHLLMTVGDIFGAGVETTTTVLKWAVLYLLHYPEVQKKIQEEMDQKIGLARHPHLSDRPLLPYLEATISEGLRIRPVSPLLIPHVSLADTSIGEYSIPKGARV 395          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C519B_DICDI (Probable cytochrome P450 519B1 OS=Dictyostelium discoideum GN=cyp519b1 PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.606e-11
Identity = 38/112 (33.93%), Postives = 61/112 (54.46%), Query Frame = 2
Query:  251 GREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKD-GLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTEL-GGYSIPAGVHV 580
            GRE+   + ++ +  + + AG+DT   T EW   +L+ N ++Q+K Y+E+++ VGKD   V    +E  PY  +++KE  R   PG   L   +I DT L  G  IP G  +
Sbjct:  273 GREK---ETVILVGMDFLLAGSDTQKATQEWFCLYLINNPDVQKKAYQELISVVGKDCKFVTSNHIENCPYFISIIKEVFRIRSPGPLGLPRISIDDTYLSNGMFIPKGTQI 381          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A1_ORYLA (Cytochrome P450 1A1 OS=Oryzias latipes GN=cyp1a1 PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.098e-11
Identity = 45/187 (24.06%), Postives = 85/187 (45.45%), Query Frame = 2
Query:    8 KDVMLITSPQLP-DFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            +D + +T    P DF+P L  L  + M++  E+  R    +  ++    A  +K    +    ++       +D    +      Q+ ++++V + +++  AG DT +T   WA+ +LV   +I++ L++EI   +G +      D   +P  DA + E  R      F + H   +DT L GY IP
Sbjct:  227 EDFVQVTGSGNPADFIPALRYLPSKAMKKFVEINNRFQSFVQKIVNEHSATYDKDNIRDITDSLIDHCEDRKLDENSNI------QMSDEKVVGIVNDLFGAGFDTISTALSWAVGYLVAYPDIEKGLFEEIKENIGLNRNPTISDRSNLPLTDAFILEIFRHSSFLPFTIPHCTTRDTSLNGYYIP 407          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C75A1_PETHY (Flavonoid 3',5'-hydroxylase 1 OS=Petunia hybrida GN=CYP75A1 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.098e-11
Identity = 35/103 (33.98%), Postives = 56/103 (54.37%), Query Frame = 2
Query:  257 EQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            E+L    I  L   + +AGTDTS++  EWA+  ++ N  I +K   E+   +G++  + E D+  +PYL A+ KET R+HP     L   + +   + GY IP
Sbjct:  284 ERLSTTNIKALLLNLFTAGTDTSSSAIEWALAEMMKNPAILKKAQAEMDQVIGRNRRLLESDIPNLPYLRAICKETFRKHPSTPLNLPRISNEPCIVDGYYIP 386          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71D7_SOLCH (Cytochrome P450 71D7 OS=Solanum chacoense GN=CYP71D7 PE=3 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.098e-11
Identity = 35/106 (33.02%), Postives = 56/106 (52.83%), Query Frame = 2
Query:  263 LGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            +    I  +  ++ +AGT+TS+TT  WAM  ++ N  +  K   ++  A        E DVE++ YL  V+KET+R H P   L+     ++TE+ GY+IP    V
Sbjct:  287 INNDNIKAVIIDLFAAGTETSSTTTVWAMAEMLKNPSVFAKAQAKVREAFRDKVTFDENDVEELKYLKLVIKETMRLHAPVPLLVPRECREETEINGYTIPVKTKV 392          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71B9_ARATH (Cytochrome P450 71B9 OS=Arabidopsis thaliana GN=CYP71B9 PE=2 SV=3)

HSP 1 Score: 68.9366 bits (167), Expect = 2.098e-11
Identity = 38/119 (31.93%), Postives = 59/119 (49.58%), Query Frame = 2
Query:  218 YVDSLFGLEPT----GREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSI 562
            +VD L  LE      G  +L    +  +   V+    +TSA T  WAM  L+ N  + +K+  EI   +    ++  +D++ +PYL  V+KET R HPP   LL    + + E+ GY I
Sbjct:  267 FVDLLLKLEKEETVLGYGKLTRNHVKAILMNVLLGAINTSAMTMTWAMAELIRNPRVMKKVQSEIRNQMINKSVITLDDIDHLPYLKMVIKETWRLHPPVPLLLPREVMSEFEINGYKI 385          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C515B_DICDI (Probable cytochrome P450 515B1 OS=Dictyostelium discoideum GN=cyp515b1 PE=2 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 2.098e-11
Identity = 52/203 (25.62%), Postives = 94/203 (46.31%), Query Frame = 2
Query:    8 KDVMLITSPQLPDFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKD--GLVKEEDVEKM----------PYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            ++++++ S    D++ +L P      ++ KE+  R  + + P +  +   +++  NP    +        Y+      +P+   ++   + + L  +++  GTD++++  EW +  L  ++ IQEKLY EI      +      E D E            P  +A VKETLRR PP    L H   +DTE+GGY IP G  +
Sbjct:  206 EELLVVLSRMPSDYIKVLRPF--ESHKKLKEICDRMSKFIKPRVDKKVELLDQ-DNPKCFID--------YLILQIRSDPSQTIKIDAIQYIVL--DLLVGGTDSTSSALEWMILFLSNHESIQEKLYNEICKNTNTNTTATTTENDNESYFPKLIEKNNYPLFNACVKETLRRSPPVPLGLPHLCSEDTEIGGYLIPKGTQI 395          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP4CU_BLAGE (Cytochrome P450 4c21 OS=Blattella germanica GN=CYP4C21 PE=2 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.740e-11
Identity = 51/183 (27.87%), Postives = 87/183 (47.54%), Query Frame = 2
Query:   41 PDFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAV-GKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHVEF 586
            P+F+  LTP +    +  K L     + +      RK    K    + ++++       ++D+L        + L + +I+      +  G DT +    W +  L  + EIQE+ YKE+     G D +    D+  M YL+ V+KE+LR HP   + +  A  +D ELGGY+IPAG +++F
Sbjct:  223 PEFIFKLTPYYWTHKKCLKVLNGFVNKIIQERKEERKK--SKVTQTSEDADIGKKKRVPFLDTLLDASEDDNK-LTDTDILEEVHTFMFEGHDTVSAAMTWLLFELGHHPEIQEEAYKEVQDIFQGSDRVPTMADLNNMNYLERVIKESLRLHPSVIYFVREAH-QDFELGGYTIPAGTNIDF 401          
The following BLAST results are available for this feature:
BLAST of CX288495 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 193
Match NameE-valueIdentityDescription
C13B1_CAEEL9.417e-1225.48Putative cytochrome P450 cyp-13B1 OS=Caenorhabditi... [more]
CP303_DROME1.606e-1131.93Probable cytochrome P450 303a1 OS=Drosophila melan... [more]
CP17A_CHICK1.606e-1130.84Steroid 17-alpha-hydroxylase/17,20 lyase OS=Gallus... [more]
C519B_DICDI1.606e-1133.93Probable cytochrome P450 519B1 OS=Dictyostelium di... [more]
CP1A1_ORYLA2.098e-1124.06Cytochrome P450 1A1 OS=Oryzias latipes GN=cyp1a1 P... [more]
C75A1_PETHY2.098e-1133.98Flavonoid 3',5'-hydroxylase 1 OS=Petunia hybrida G... [more]
C71D7_SOLCH2.098e-1133.02Cytochrome P450 71D7 OS=Solanum chacoense GN=CYP71... [more]
C71B9_ARATH2.098e-1131.93Cytochrome P450 71B9 OS=Arabidopsis thaliana GN=CY... [more]
C515B_DICDI2.098e-1125.62Probable cytochrome P450 515B1 OS=Dictyostelium di... [more]
CP4CU_BLAGE2.740e-1127.87Cytochrome P450 4c21 OS=Blattella germanica GN=CYP... [more]

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Properties
Property NameValue
Genbank descriptionC02001A10SK IF1 Citrus clementina cDNA clone C02001A10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX288495 ID=CX288495; Name=CX288495; organism=Citrus clementina; type=EST; length=594bp
CATACTAAAAGATGTAATGCTGATAACATCACCACAGCTTCCGGATTTCT
TGCCGATTCTCACTCCATTATTTCGCAGGCAAATGAGAGAAGCAAAGGAG
TTGAGGAAGAGACAAATGGAGTGCTTGGTTCCACTGATAAGGAACAGAAA
AGCTTTTGTAGAGAAAGGCCAAAACCCTAACTCAGAGTCAGAGATGGTGA
GTCCAATTGGTGCTGCTTATGTGGATTCATTGTTTGGGCTGGAGCCAACG
GGGAGAGAGCAGCTGGGGGAACAAGAGATTGTGACATTGTGTTCTGAAGT
GATCAGTGCAGGCACTGACACTAGCGCAACAACGGCCGAATGGGCAATGC
ATCACTTGGTGCTAAACCAGGAGATTCAAGAAAAGTTGTATAAAGAAATT
GTAGCTGCTGTGGGGAAAGATGGGCTTGTTAAAGAAGAAGATGTCGAGAA
AATGCCTTATCTCGATGCTGTCGTGAAGGAAACTCTCCGGCGGCACCCTC
CTGGCCATTTTCTGCTGTCTCACGCTGCCATAAAGGATACTGAGCTTGGC
GGGTACTCCATCCCGGCAGGTGTTCACGTGGAATTCTACACTGC
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