CX288495

Overview
NameCX288495
Unique NameCX288495
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length594
Libraries
Library NameType
IF1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71E1_SORBI (4-hydroxyphenylacetaldehyde oxime monooxygenase OS=Sorghum bicolor GN=CYP71E1 PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.188e-14
Identity = 47/127 (37.01%), Postives = 62/127 (48.82%), Query Frame = 2
Query:  209 GAAYVDSLFGL--EPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDG-LVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            G   VD L  L  E  G  +     +  +  +      DTS+ T  WAM  L+   ++  K   E+ AAVG D   V  ED  K+PYL  VVKETLR HPP   L+    ++DT + GY +PA   V
Sbjct:  293 GGDLVDVLINLCKEHDGTLRFTRDHVKAIVLDTFIGAIDTSSVTILWAMSELMRKPQVLRKAQAEVRAAVGDDKPRVNSEDAAKIPYLKMVVKETLRLHPPATLLVPRETMRDTTICGYDVPANTRV 419          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71BP_ARATH (Cytochrome P450 71B25 OS=Arabidopsis thaliana GN=CYP71B25 PE=2 SV=1)

HSP 1 Score: 79.7221 bits (195), Expect = 1.188e-14
Identity = 47/136 (34.56%), Postives = 70/136 (51.47%), Query Frame = 2
Query:  161 EKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVG-KDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            +K   P   S++V  +    +D +   E     +L    +  +  ++  AG DTSA T  WAM  LV N  + +K+  EI + +G K   ++EEDV K+ YL  V+KETLR HP    LL    + D ++ GY IP
Sbjct:  260 QKQNQPQDRSDIVGSL----LDMIHNQEQDKSFKLTIDHLKGITQDIFLAGIDTSAITMIWAMAELVNNPRVMKKVQDEIRSCIGIKKERIEEEDVGKLQYLKLVIKETLRLHPAAPLLLPRETMADIKIQGYDIP 391          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C83A1_ARATH (Cytochrome P450 83A1 OS=Arabidopsis thaliana GN=CYP83A1 PE=1 SV=2)

HSP 1 Score: 79.337 bits (194), Expect = 1.552e-14
Identity = 45/146 (30.82%), Postives = 77/146 (52.74%), Query Frame = 2
Query:  155 FVEKGQNPNSESEMVSPIGAAYVDSLFGL--EPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDG--LVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            ++++  N   + + V P   + +D L G+  E     +     +  +  +++ AGTDT+A    W M +L+   ++ +K   E+   + + G   V E+DV+ +PY  A+VKETLR  P    L+  A I+DT++ GY IPAG  V
Sbjct:  247 YIQEVVNETLDPKRVKPETESMIDLLMGIYKEQPFASEFTVDNVKAVILDIVVAGTDTAAAAVVWGMTYLMKYPQVLKKAQAEVREYMKEKGSTFVTEDDVKNLPYFRALVKETLRIEPVIPLLIPRACIQDTKIAGYDIPAGTTV 392          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C75A1_PINTA (Cytochrome P450 750A1 OS=Pinus taeda GN=CYP750A1 PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.552e-14
Identity = 41/96 (42.71%), Postives = 57/96 (59.38%), Query Frame = 2
Query:  278 IVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            I  +  E+ SAG +TSA   EWAM  L+ +    +KL +EI + VG+ G VKE D+  + YL  VVKETLR +P     L H +++   +GGY IP
Sbjct:  310 IKAITYELFSAGMETSANVLEWAMSELLRHPHAMKKLQQEIESVVGQQGTVKESDLASIVYLHCVVKETLRLYPSLPLALPHESLEAVTVGGYYIP 405          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71AL_ARATH (Cytochrome P450 71A21 OS=Arabidopsis thaliana GN=CYP71A21 PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.552e-14
Identity = 50/126 (39.68%), Postives = 68/126 (53.97%), Query Frame = 2
Query:  218 YVDSLFGLEPTGREQLGEQEIVTLCS-----EVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            +VD L  ++   RE+    EI  LC      +V+ AGTD+S    +WAM  L+ + E    L +E+      +  V EED++ M YL AV+KET R HPP   L  H +I+D  LG Y IPAG  V
Sbjct:  260 FVDVLLRIQ---REKSIGFEIDRLCIKAIVLDVLVAGTDSSYALMDWAMTELLRHPECLRTLQEEVRTICKGNLSVSEEDIQNMSYLKAVIKETTRLHPPLPLLAPHESIQDVILGDYHIPAGTQV 382          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C71AE_ARATH (Cytochrome P450 71A14 OS=Arabidopsis thaliana GN=CYP71A14 PE=2 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.552e-14
Identity = 52/124 (41.94%), Postives = 66/124 (53.23%), Query Frame = 2
Query:  218 YVDSLFGLEPTGRE--QLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGL-VKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            +VD L  LE   R   Q+   +I  L  ++  AGT+T+    EW M  L+ + E  +KL  EI A   K  L + EEDVE M YL AVVKE LR HPP   L+     +D +L GY I AG  V
Sbjct:  267 FVDVLLSLERHERNGVQIRRSDIKFLILDMFLAGTETTYALLEWIMTELIRHPECMKKLQDEIRAKATKLILYISEEDVEDMKYLKAVVKEVLRLHPPLPLLVPRELSEDIKLKGYDIAAGTQV 390          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A1_STECH (Cytochrome P450 1A1 OS=Stenotomus chrysops GN=cyp1a1 PE=2 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 2.027e-14
Identity = 50/182 (27.47%), Postives = 84/182 (46.15%), Query Frame = 2
Query:   20 LITSPQLPDFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            ++ S    DF+PIL  L    M++   +  R    +  ++       +K    +    ++       +D           Q+ +++IV + +++  AG DT +T   W++ +LV   EIQE+LY+E+   VG D      D  K+P+L+A + ET R      F + H   KDT L GY IP
Sbjct:  232 VVASGNPADFIPILQYLPSTTMKKFLNINDRFNTFVQKIVSEHYTTFDKDNIRDITDSLIDHCEDRKLDE------NSNVQMSDEKIVGIVNDLFGAGFDTISTALSWSVMYLVAYPEIQERLYQEMNETVGPDRTPCLSDKPKLPFLEAFILETFRHSSFLPFTIPHCTSKDTSLNGYFIP 407          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A2_CAVPO (Cytochrome P450 1A2 OS=Cavia porcellus GN=CYP1A2 PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 2.648e-14
Identity = 48/174 (27.59%), Postives = 83/174 (47.70%), Query Frame = 2
Query:   44 DFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            DF PIL  L    +++ K+  KR ++ L   ++      +K          V  I +A             + +  ++IV L +++  AG DT  T   W++ +LV   EIQ+K++KE+ A +G+D   +  D  ++PY++A + E  R      F + H   +DT L G+ IP
Sbjct:  237 DFFPILRYLPNPMLQQFKDFNKRFLQFLQKTVQEHYQDFDKNH--------VQDIASALFKHSEESPHVNGDLIPRKKIVNLVNDIFGAGFDTVTTAISWSLLYLVTKPEIQKKIHKELDAVIGRDRKPRLADRPQLPYMEAFILEVFRYSSFLPFTIPHCTTRDTILNGFYIP 402          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: C80A1_BERST (Berbamunine synthase OS=Berberis stolonifera GN=CYP80A1 PE=1 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 2.648e-14
Identity = 37/104 (35.58%), Postives = 60/104 (57.69%), Query Frame = 2
Query:  269 EQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIPAGVHV 580
            E +I  +  E    G+DT++   EWA+  L+ N +   KL +E+   VG+   VKE    ++PYL A VKET+R +PP   ++ H  ++  ++ GY+IP G+ V
Sbjct:  277 EHQINAMFMETFGPGSDTNSNIIEWALAQLIKNPDKLAKLREELDRVVGRSSTVKESHFSELPYLQACVKETMRLYPPISIMIPHRCMETCQVMGYTIPKGMDV 380          
BLAST of CX288495 vs. ExPASy Swiss-Prot
Match: CP1A2_HUMAN (Cytochrome P450 1A2 OS=Homo sapiens GN=CYP1A2 PE=1 SV=3)

HSP 1 Score: 78.1814 bits (191), Expect = 3.458e-14
Identity = 51/174 (29.31%), Postives = 86/174 (49.43%), Query Frame = 2
Query:   44 DFLPILTPLFRRQMREAKELRKRQMECLVPLIRNRKAFVEKGQNPNSESEMVSPIGAAYVDSLFGLEPTGREQLGEQEIVTLCSEVISAGTDTSATTAEWAMHHLVLNQEIQEKLYKEIVAAVGKDGLVKEEDVEKMPYLDAVVKETLRRHPPGHFLLSHAAIKDTELGGYSIP 565
            DF PIL  L    ++  K   +R +  L   ++      +K    NS  ++    GA +  S  G   +G   + +++IV L +++  AG DT  T   W++ +LV   EIQ K+ KE+   +G++   +  D  ++PYL+A + ET R      F + H+  +DT L G+ IP
Sbjct:  237 DFFPILRYLPNPALQRFKAFNQRFLWFLQKTVQEHYQDFDK----NSVRDIT---GALFKHSKKGPRASGN-LIPQEKIVNLVNDIFGAGFDTVTTAISWSLMYLVTKPEIQRKIQKELDTVIGRERRPRLSDRPQLPYLEAFILETFRHSSFLPFTIPHSTTRDTTLNGFYIP 402          
The following BLAST results are available for this feature:
BLAST of CX288495 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 193
Match NameE-valueIdentityDescription
C71E1_SORBI1.188e-1437.014-hydroxyphenylacetaldehyde oxime monooxygenase OS... [more]
C71BP_ARATH1.188e-1434.56Cytochrome P450 71B25 OS=Arabidopsis thaliana GN=C... [more]
C83A1_ARATH1.552e-1430.82Cytochrome P450 83A1 OS=Arabidopsis thaliana GN=CY... [more]
C75A1_PINTA1.552e-1442.71Cytochrome P450 750A1 OS=Pinus taeda GN=CYP750A1 P... [more]
C71AL_ARATH1.552e-1439.68Cytochrome P450 71A21 OS=Arabidopsis thaliana GN=C... [more]
C71AE_ARATH1.552e-1441.94Cytochrome P450 71A14 OS=Arabidopsis thaliana GN=C... [more]
CP1A1_STECH2.027e-1427.47Cytochrome P450 1A1 OS=Stenotomus chrysops GN=cyp1... [more]
CP1A2_CAVPO2.648e-1427.59Cytochrome P450 1A2 OS=Cavia porcellus GN=CYP1A2 P... [more]
C80A1_BERST2.648e-1435.58Berbamunine synthase OS=Berberis stolonifera GN=CY... [more]
CP1A2_HUMAN3.458e-1429.31Cytochrome P450 1A2 OS=Homo sapiens GN=CYP1A2 PE=1... [more]

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Properties
Property NameValue
Genbank descriptionC02001A10SK IF1 Citrus clementina cDNA clone C02001A10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX288495 ID=CX288495; Name=CX288495; organism=Citrus clementina; type=EST; length=594bp
CATACTAAAAGATGTAATGCTGATAACATCACCACAGCTTCCGGATTTCT
TGCCGATTCTCACTCCATTATTTCGCAGGCAAATGAGAGAAGCAAAGGAG
TTGAGGAAGAGACAAATGGAGTGCTTGGTTCCACTGATAAGGAACAGAAA
AGCTTTTGTAGAGAAAGGCCAAAACCCTAACTCAGAGTCAGAGATGGTGA
GTCCAATTGGTGCTGCTTATGTGGATTCATTGTTTGGGCTGGAGCCAACG
GGGAGAGAGCAGCTGGGGGAACAAGAGATTGTGACATTGTGTTCTGAAGT
GATCAGTGCAGGCACTGACACTAGCGCAACAACGGCCGAATGGGCAATGC
ATCACTTGGTGCTAAACCAGGAGATTCAAGAAAAGTTGTATAAAGAAATT
GTAGCTGCTGTGGGGAAAGATGGGCTTGTTAAAGAAGAAGATGTCGAGAA
AATGCCTTATCTCGATGCTGTCGTGAAGGAAACTCTCCGGCGGCACCCTC
CTGGCCATTTTCTGCTGTCTCACGCTGCCATAAAGGATACTGAGCTTGGC
GGGTACTCCATCCCGGCAGGTGTTCACGTGGAATTCTACACTGC
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