CX288586

Overview
NameCX288586
Unique NameCX288586
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length527
Libraries
Library NameType
IF1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CX288586 vs. ExPASy Swiss-Prot
Match: ENO2_DESHY (Enolase 2 OS=Desulfitobacterium hafniense (strain Y51) GN=eno2 PE=3 SV=1)

HSP 1 Score: 98.5969 bits (244), Expect = 1.603e-24
Identity = 55/121 (45.45%), Postives = 78/121 (64.46%), Query Frame = 3 
Query:   21 AIAKAGYT-GK-VVIGMDVAASEFYGSDKTYDLNFKEENNDGSQKISGDALKDLYKSFISDYPIVSIEDPFDQDDWEHYAKLTSEVGEKVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKV 377
            AI +AGYT GK + + +DVAA+E Y   K Y L       +G  K + D +   Y+  +  YPIVSIED   ++DWE + KLT ++G K+Q+VGDDL VTNP R+ + IKEK  N++L+K+
Sbjct:  225 AIQRAGYTPGKDIALALDVAATELYRDGK-YHLE-----GEGLTK-TADEMIAYYEDLVEKYPIVSIEDGLSEEDWEGWKKLTEKLGSKIQLVGDDLFVTNPARLARGIKEKCANSILIKL 338

HSP 2 Score: 33.4982 bits (75), Expect = 1.603e-24
Identity = 12/23 (52.17%), Postives = 22/23 (95.65%), Query Frame = 2 
Query:  458 KVNQIGSVTESIEAVRMSKQAGW 526
            K+NQIG++TE+++A+ M+K+AG+
Sbjct:  337 KLNQIGTLTETLDAIEMAKRAGY 359
[Close]
BLAST of CX288586 vs. ExPASy Swiss-Prot
Match: ENO_PYRFU (Enolase OS=Pyrococcus furiosus GN=eno PE=3 SV=1)

HSP 1 Score: 106.686 bits (265), Expect = 2.085e-24
Identity = 61/130 (46.92%), Postives = 79/130 (60.77%), Query Frame = 3 
Query:    3 LELLNTAIAKAGYT--GKVVIGMDVAASEFYGSDKTYDLNFKEENND---GSQKISGDALKDLYKSFISDYPIVSIEDPFDQDDWEHYAKLTSEVGEKVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKV 377
            L++L  AI +AGY    ++ + +DVA+SE           F EE      G ++     L +LYK   S YPIVSIEDPF ++DWE +  +T E+G KVQIVGDDL VTNPKR+ K I+    NALLLKV
Sbjct:  222 LDVLIKAIEEAGYKPGDEIALALDVASSEL----------FNEETGKYVVGGKEYDRGELLELYKDLTSTYPIVSIEDPFHEEDWEGFVMITKELGHKVQIVGDDLFVTNPKRLRKGIELGAANALLLKV 341

HSP 2 Score: 25.0238 bits (53), Expect = 2.085e-24
Identity = 10/23 (43.48%), Postives = 19/23 (82.61%), Query Frame = 2 
Query:  458 KVNQIGSVTESIEAVRMSKQAGW 526
            KVNQIG+++E+++A   + +AG+
Sbjct:  340 KVNQIGTLSEAMDAAFTAFRAGY 362
[Close]
BLAST of CX288586 vs. ExPASy Swiss-Prot
Match: ENO_LACCB (Enolase OS=Lactobacillus casei (strain BL23) GN=eno PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 2.713e-24
Identity = 53/127 (41.73%), Postives = 78/127 (61.42%), Query Frame = 3 
Query:    6 ELLNTAIAKAGYT--GKVVIGMDVAASEFYGSD-KTYDLNFKEENNDGSQKISGDALKDLYKSFISDYPIVSIEDPFDQDDWEHYAKLTSEVGEKVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKV 377
            ELL  AI KAGY     + +  DVAASE Y ++ KTY   +   +    +K + +   D+   +I+ YPIVS+EDP D++DWE +   T ++G+KVQIVGDDL VTN   ++K I     N++L+K+
Sbjct:  220 ELLVEAIKKAGYKPGDDIALAFDVAASEMYDAESKTYTTKWSNPD----KKYTTEEWTDMIDGYINKYPIVSVEDPIDENDWEGWQTFTKKMGDKVQIVGDDLFVTNTDYLKKGIDMGVANSILIKL 342

HSP 2 Score: 34.2686 bits (77), Expect = 2.713e-24
Identity = 13/23 (56.52%), Postives = 21/23 (91.30%), Query Frame = 2 
Query:  458 KVNQIGSVTESIEAVRMSKQAGW 526
            K+NQIG++TE+ EA+ M+K+AG+
Sbjct:  341 KLNQIGTLTETFEAIEMAKEAGY 363
[Close]
BLAST of CX288586 vs. ExPASy Swiss-Prot
Match: ENO_LACC3 (Enolase OS=Lactobacillus casei (strain ATCC 334) GN=eno PE=3 SV=1)

HSP 1 Score: 97.0561 bits (240), Expect = 2.713e-24
Identity = 53/127 (41.73%), Postives = 78/127 (61.42%), Query Frame = 3 
Query:    6 ELLNTAIAKAGYT--GKVVIGMDVAASEFYGSD-KTYDLNFKEENNDGSQKISGDALKDLYKSFISDYPIVSIEDPFDQDDWEHYAKLTSEVGEKVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKV 377
            ELL  AI KAGY     + +  DVAASE Y ++ KTY   +   +    +K + +   D+   +I+ YPIVS+EDP D++DWE +   T ++G+KVQIVGDDL VTN   ++K I     N++L+K+
Sbjct:  220 ELLVEAIKKAGYKPGDDIALAFDVAASEMYDAESKTYTTKWSNPD----KKYTTEEWTDMIDGYINKYPIVSVEDPIDENDWEGWQTFTKKMGDKVQIVGDDLFVTNTDYLKKGIDMGVANSILIKL 342

HSP 2 Score: 34.2686 bits (77), Expect = 2.713e-24
Identity = 13/23 (56.52%), Postives = 21/23 (91.30%), Query Frame = 2 
Query:  458 KVNQIGSVTESIEAVRMSKQAGW 526
            K+NQIG++TE+ EA+ M+K+AG+
Sbjct:  341 KLNQIGTLTETFEAIEMAKEAGY 363
[Close]
BLAST of CX288586 vs. ExPASy Swiss-Prot
Match: ENO_XANC5 (Enolase OS=Xanthomonas campestris pv. vesicatoria (strain 85-10) GN=eno PE=3 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 2.714e-24
Identity = 55/127 (43.31%), Postives = 80/127 (62.99%), Query Frame = 3 
Query:    3 LELLNTAIAKAGYTG--KVVIGMDVAASEFYGSDKTYDLNFKEENNDGSQKISGDALKDLYKSFISDYPIVSIEDPFDQDDWEHYAKLTSEVGEKVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKV 377
            L+ +  AI KAGYT    +++G+DVA+SEFY + K    N   EN    ++++ +   D    +++ YPI+SIED   +DDW  +  LT  VG+KVQ+VGDDL VTNPK  ++ I   T NA+L+KV
Sbjct:  221 LDTILEAIGKAGYTAGEDILLGLDVASSEFYDNGK---YNLVGEN----KRLTSEQFVDFLADWVAQYPIISIEDGLAEDDWAGWKLLTERVGKKVQLVGDDLFVTNPKIFKQGIDSGTANAILIKV 340

HSP 2 Score: 29.261 bits (64), Expect = 2.714e-24
Identity = 12/21 (57.14%), Postives = 18/21 (85.71%), Query Frame = 2 
Query:  458 KVNQIGSVTESIEAVRMSKQA 520
            KVNQIG++TE++EA+ M+  A
Sbjct:  339 KVNQIGTLTETLEAIAMAHAA 359
[Close]
BLAST of CX288586 vs. ExPASy Swiss-Prot
Match: ENO_FRASC (Enolase OS=Frankia sp. (strain CcI3) GN=eno PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.714e-24
Identity = 56/127 (44.09%), Postives = 80/127 (62.99%), Query Frame = 3 
Query:    3 LELLNTAIAKAGYT--GKVVIGMDVAASEFYGSDKTYDLNFKEENNDGSQKISGDALKDLYKSFISDYPIVSIEDPFDQDDWEHYAKLTSEVGEKVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKV 377
            L+L+   I KAG+     + + +DVA++EFY +D +Y         +G+ + S + L D Y   +S YPIVSIEDP  +DDW+ +  LT  +G KVQ+VGDDL VTNP+R+ + I  K  NALL+KV
Sbjct:  218 LDLIAEGIDKAGFALGTDIALALDVASTEFY-ADGSYTF-------EGNSR-SAEYLSDYYAELVSAYPIVSIEDPLAEDDWDGWVALTERLGGKVQLVGDDLFVTNPERLARGIALKAANALLVKV 335

HSP 2 Score: 30.4166 bits (67), Expect = 2.714e-24
Identity = 11/23 (47.83%), Postives = 21/23 (91.30%), Query Frame = 2 
Query:  458 KVNQIGSVTESIEAVRMSKQAGW 526
            KVNQIG++TE+++AV ++ ++G+
Sbjct:  334 KVNQIGTLTETLDAVNLAHRSGY 356
[Close]
BLAST of CX288586 vs. ExPASy Swiss-Prot
Match: ENO_CYAP4 (Enolase OS=Cyanothece sp. (strain PCC 7425 / ATCC 29141) GN=eno PE=3 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 2.714e-24
Identity = 61/127 (48.03%), Postives = 83/127 (65.35%), Query Frame = 3 
Query:    3 LELLNTAIAKAGYTG--KVVIGMDVAASEFYGSDKTYDLNFKEENNDGSQKISGDALKDLYKSFISDYPIVSIEDPFDQDDWEHYAKLTSEVGEKVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKV 377
            L+LL TAIAKAG+    ++ + +DVAASEFY  D  Y         DG+   S   L D   +    YPIVSIED   +DDW+H+ +LTS++G +VQ+VGDDL VTNP R++K IKE+  NA+L+K+
Sbjct:  221 LDLLMTAIAKAGFKPGEEISLALDVAASEFY-QDGQYSY-------DGAAH-SPSELIDYLATLSEQYPIVSIEDGLQEDDWQHWQQLTSKIGHRVQLVGDDLFVTNPIRLDKGIKERAGNAILIKL 338

HSP 2 Score: 24.2534 bits (51), Expect = 2.714e-24
Identity = 8/18 (44.44%), Postives = 16/18 (88.89%), Query Frame = 2 
Query:  458 KVNQIGSVTESIEAVRMS 511
            K+NQIGS+TE+++ + ++
Sbjct:  337 KLNQIGSLTETLQTIDLA 354
[Close]
BLAST of CX288586 vs. ExPASy Swiss-Prot
Match: ENO_MYCA9 (Enolase OS=Mycobacterium abscessus (strain ATCC 19977 / DSM 44196) GN=eno PE=3 SV=1)

HSP 1 Score: 101.293 bits (251), Expect = 3.531e-24
Identity = 53/127 (41.73%), Postives = 79/127 (62.20%), Query Frame = 3 
Query:    3 LELLNTAIAKAGYT--GKVVIGMDVAASEFYGSDKTYDLNFKEENNDGSQKISGDALKDLYKSFISDYPIVSIEDPFDQDDWEHYAKLTSEVGEKVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKV 377
            L+L++TAI   G      V + +DVAA+EFY +   Y  +F++E     Q      +   Y   +  YP+VSIEDP  +DDW+ +  LT+ +G++VQ+VGDDL VTNP+R+E+ I+    NALL+KV
Sbjct:  218 LDLISTAIEATGLKLGADVALALDVAATEFYSAADGY--SFEKEKRTAEQ------MGAFYAELLDAYPLVSIEDPLSEDDWDGWVALTTAIGDRVQLVGDDLFVTNPERLEEGIERGAANALLVKV 336

HSP 2 Score: 29.6462 bits (65), Expect = 3.531e-24
Identity = 11/23 (47.83%), Postives = 20/23 (86.96%), Query Frame = 2 
Query:  458 KVNQIGSVTESIEAVRMSKQAGW 526
            KVNQIG++TE+++AV ++  +G+
Sbjct:  335 KVNQIGTLTETLDAVTLAHSSGY 357
[Close]
BLAST of CX288586 vs. ExPASy Swiss-Prot
Match: ENO_THESQ (Enolase OS=Thermotoga sp. (strain RQ2) GN=eno PE=3 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 3.532e-24
Identity = 57/128 (44.53%), Postives = 81/128 (63.28%), Query Frame = 3 
Query:    3 LELLNTAIAKAGYTG--KVVIGMDVAASEFYGSDK-TYDLNFKEENNDGSQKISGDALKDLYKSFISDYPIVSIEDPFDQDDWEHYAKLTSEVGEKVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKV 377
            +++L  AI KAGY    +V I +D AAS FY  +K  Y +       DG +K S + L   Y+  ++ YPI+SIEDPF ++DW+ + + T  VG KVQIVGDDL VTN KR+ K I+ K  N++L+K+
Sbjct:  220 IKVLIEAIEKAGYKPGEEVFIALDCAASSFYDEEKGVYYV-------DGEEK-SSEVLMGYYEELVAKYPIISIEDPFAEEDWDAFVEFTKRVGNKVQIVGDDLYVTNVKRLSKGIELKATNSILIKL 339

HSP 2 Score: 28.8758 bits (63), Expect = 3.532e-24
Identity = 11/20 (55.00%), Postives = 19/20 (95.00%), Query Frame = 2 
Query:  458 KVNQIGSVTESIEAVRMSKQ 517
            K+NQIG+VTE+++AV M+++
Sbjct:  338 KLNQIGTVTETLDAVEMAQK 357
[Close]
BLAST of CX288586 vs. ExPASy Swiss-Prot
Match: ENO_THEP1 (Enolase OS=Thermotoga petrophila (strain RKU-1 / ATCC BAA-488 / DSM 13995) GN=eno PE=3 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 3.532e-24
Identity = 57/128 (44.53%), Postives = 81/128 (63.28%), Query Frame = 3 
Query:    3 LELLNTAIAKAGYTG--KVVIGMDVAASEFYGSDK-TYDLNFKEENNDGSQKISGDALKDLYKSFISDYPIVSIEDPFDQDDWEHYAKLTSEVGEKVQIVGDDLLVTNPKRVEKAIKEKTCNALLLKV 377
            +++L  AI KAGY    +V I +D AAS FY  +K  Y +       DG +K S + L   Y+  ++ YPI+SIEDPF ++DW+ + + T  VG KVQIVGDDL VTN KR+ K I+ K  N++L+K+
Sbjct:  220 IKVLIEAIEKAGYKPGEEVFIALDCAASSFYDEEKGVYYV-------DGEEK-SSEVLMGYYEELVAKYPIISIEDPFAEEDWDAFVEFTKRVGNKVQIVGDDLYVTNVKRLSKGIELKATNSILIKL 339

HSP 2 Score: 28.8758 bits (63), Expect = 3.532e-24
Identity = 11/20 (55.00%), Postives = 19/20 (95.00%), Query Frame = 2 
Query:  458 KVNQIGSVTESIEAVRMSKQ 517
            K+NQIG+VTE+++AV M+++
Sbjct:  338 KLNQIGTVTETLDAVEMAQK 357
The following BLAST results are available for this feature:
BLAST of CX288586 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
ENO2_DESHY1.603e-2445.45Enolase 2 OS=Desulfitobacterium hafniense (strain ... [more]
ENO_PYRFU2.085e-2446.92Enolase OS=Pyrococcus furiosus GN=eno PE=3 SV=1[more]
ENO_LACCB2.713e-2441.73Enolase OS=Lactobacillus casei (strain BL23) GN=en... [more]
ENO_LACC32.713e-2441.73Enolase OS=Lactobacillus casei (strain ATCC 334) G... [more]
ENO_XANC52.714e-2443.31Enolase OS=Xanthomonas campestris pv. vesicatoria ... [more]
ENO_FRASC2.714e-2444.09Enolase OS=Frankia sp. (strain CcI3) GN=eno PE=3 S... [more]
ENO_CYAP42.714e-2448.03Enolase OS=Cyanothece sp. (strain PCC 7425 / ATCC ... [more]
ENO_MYCA93.531e-2441.73Enolase OS=Mycobacterium abscessus (strain ATCC 19... [more]
ENO_THESQ3.532e-2444.53Enolase OS=Thermotoga sp. (strain RQ2) GN=eno PE=3... [more]
ENO_THEP13.532e-2444.53Enolase OS=Thermotoga petrophila (strain RKU-1 / A... [more]

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Properties
Property NameValue
Genbank descriptionC02002A12SK IF1 Citrus clementina cDNA clone C02002A12, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX288586 ID=CX288586; Name=CX288586; organism=Citrus clementina; type=EST; length=527bp
GACTTGAATTGCTCAACACAGCCATTGCTAAAGCCGGTTACACAGGCAAA
GTTGTCATTGGAATGGATGTTGCTGCATCTGAATTCTATGGCTCAGATAA
GACTTATGATCTGAACTTCAAGGAAGAGAACAATGATGGTTCACAAAAGA
TCTCAGGAGATGCACTCAAAGATCTCTATAAGTCATTCATCTCTGATTAT
CCTATTGTATCAATTGAAGATCCATTTGACCAAGACGACTGGGAGCACTA
TGCCAAGCTAACCAGTGAGGTCGGAGAAAAAGTACAAATTGTTGGAGATG
ATCTTTTGGTCACCAATCCTAAGAGGGTTGAGAAGGCTATCAAAGAGAAG
ACCTGCAACGCCCTCCTTCTCAAGGTACTTCTTTAGTCCTTCTTTATTTG
GTCATTGGTTTACGACATTGTAAATGAAAATCTGTTCCTTCTCTCTGTTC
TGGTGGAAAGGTTAATCAAATTGGATCTGTAACTGAGAGCATTGAAGCCG
TCAGAATGTCCAAGCAAGCTGGGTGGG
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