CX290139

Overview
NameCX290139
Unique NameCX290139
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length721
Libraries
Library NameType
IF1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CX290139 vs. ExPASy Swiss-Prot
Match: EF1A_CHICK (Elongation factor 1-alpha 1 OS=Gallus gallus GN=EEF1A PE=2 SV=1)

HSP 1 Score: 371.703 bits (953), Expect = 2.189e-102
Identity = 187/248 (75.40%), Postives = 208/248 (83.87%), Query Frame = 1 
Query:   13 FVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGV 720
            FVPISG+ GDNM+E S+N+ W+KG             TLL+ALD I  P RP+DKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTF P  +TTEVKSVEMHHEAL EALPGDNVGFNVKNV+VKD++RG VA +SK+DP  EAA FT+QVII+NHPGQI  GYAPVLDCHT+HIA KF+EL  KIDRRSGK+LE  PKFLK+GDA +V MIP KPM VE+FSDYPPLGRFAVRDMRQTVAVGV
Sbjct:  190 FVPISGWNGDNMLEPSSNMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMIPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGV 437
[Close]
BLAST of CX290139 vs. ExPASy Swiss-Prot
Match: EF1A_CAEEL (Elongation factor 1-alpha OS=Caenorhabditis elegans GN=eft-3 PE=2 SV=1)

HSP 1 Score: 371.703 bits (953), Expect = 2.189e-102
Identity = 179/249 (71.89%), Postives = 212/249 (85.14%), Query Frame = 1 
Query:   10 PFVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGV 720
            PFVPISGF GDNM+E S+N+ W+KG             TLL+ALD+I  P+RP+D+PLRLPLQDVYKIGGIGTVPVGRVETG++KPGMVVTF P  +TTEVKSVEMHHE+L EA+PGDNVGFNVKNV+VKD++RG V S+SK DPA+EA  F +QVIIMNHPGQI NGY PVLDCHT+HIA KF+EL  K+DRR+GK++E  PKFLK+GDAG+V++IPTKP+ VE+F+DY PLGRFAVRDMRQTVAVGV
Sbjct:  189 PFVPISGFNGDNMLEVSSNMPWFKGWAVERKEGNASGKTLLEALDSIIPPQRPTDRPLRLPLQDVYKIGGIGTVPVGRVETGIIKPGMVVTFAPQNVTTEVKSVEMHHESLPEAVPGDNVGFNVKNVSVKDIRRGSVCSDSKQDPAKEARTFHAQVIIMNHPGQISNGYTPVLDCHTAHIACKFNELKEKVDRRTGKKVEDFPKFLKSGDAGIVELIPTKPLCVESFTDYAPLGRFAVRDMRQTVAVGV 437
[Close]
BLAST of CX290139 vs. ExPASy Swiss-Prot
Match: EF1A2_RHIRA (Elongation factor 1-alpha OS=Rhizomucor racemosus GN=TEF-2 PE=3 SV=1)

HSP 1 Score: 371.318 bits (952), Expect = 2.859e-102
Identity = 178/249 (71.49%), Postives = 210/249 (84.34%), Query Frame = 1 
Query:   10 PFVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGV 720
            PFVPISG+ GDNM++ STN+ W+KG             TLL+A+D I  P RPSDKPLRLPLQDVYKIGGIGTVPVGRVETG +K GMVV F P+ +TTEVKSVEMHHE L E LPGDNVGFNVKNV+VKD++RG V S+SK+DPA+E+A+FT+QVII+NHPGQI  GYAPVLDCHT+HIA KFSEL+ KIDRRSGK++E  PKF+K+GD+ +VKM+P+KPM VE ++DYPPLGRFAVRDMRQTVAVGV
Sbjct:  187 PFVPISGWHGDNMLDESTNMPWFKGWNKETKAGSKTGKTLLEAIDAIEPPVRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGTIKAGMVVNFAPAAVTTEVKSVEMHHETLTEGLPGDNVGFNVKNVSVKDIRRGNVCSDSKNDPAKESASFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFSELIEKIDRRSGKKMEDSPKFVKSGDSAIVKMVPSKPMCVEAYTDYPPLGRFAVRDMRQTVAVGV 435
[Close]
BLAST of CX290139 vs. ExPASy Swiss-Prot
Match: EF1A1_RHIRA (Elongation factor 1-alpha OS=Rhizomucor racemosus GN=TEF-1 PE=3 SV=1)

HSP 1 Score: 371.318 bits (952), Expect = 2.859e-102
Identity = 178/249 (71.49%), Postives = 210/249 (84.34%), Query Frame = 1 
Query:   10 PFVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGV 720
            PFVPISG+ GDNM++ STN+ W+KG             TLL+A+D I  P RPSDKPLRLPLQDVYKIGGIGTVPVGRVETG +K GMVV F P+ +TTEVKSVEMHHE L E LPGDNVGFNVKNV+VKD++RG V S+SK+DPA+E+A+FT+QVII+NHPGQI  GYAPVLDCHT+HIA KFSEL+ KIDRRSGK++E  PKF+K+GD+ +VKM+P+KPM VE ++DYPPLGRFAVRDMRQTVAVGV
Sbjct:  187 PFVPISGWHGDNMLDESTNMPWFKGWNKETKAGSKTGKTLLEAIDAIEPPVRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGTIKAGMVVNFAPAAVTTEVKSVEMHHETLTEGLPGDNVGFNVKNVSVKDIRRGNVCSDSKNDPAKESASFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFSELIEKIDRRSGKKMEDSPKFVKSGDSAIVKMVPSKPMCVEAYTDYPPLGRFAVRDMRQTVAVGV 435
[Close]
BLAST of CX290139 vs. ExPASy Swiss-Prot
Match: EF1A1_RAT (Elongation factor 1-alpha 1 OS=Rattus norvegicus GN=Eef1a1 PE=2 SV=1)

HSP 1 Score: 370.933 bits (951), Expect = 3.734e-102
Identity = 186/248 (75.00%), Postives = 207/248 (83.47%), Query Frame = 1 
Query:   13 FVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGV 720
            FVPISG+ GDNM+E S N+ W+KG             TLL+ALD I  P RP+DKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTF P  +TTEVKSVEMHHEAL EALPGDNVGFNVKNV+VKD++RG VA +SK+DP  EAA FT+QVII+NHPGQI  GYAPVLDCHT+HIA KF+EL  KIDRRSGK+LE  PKFLK+GDA +V M+P KPM VE+FSDYPPLGRFAVRDMRQTVAVGV
Sbjct:  190 FVPISGWNGDNMLEPSANMPWFKGWKVTRKDGSASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGV 437
[Close]
BLAST of CX290139 vs. ExPASy Swiss-Prot
Match: EF1A1_RABIT (Elongation factor 1-alpha 1 OS=Oryctolagus cuniculus GN=EEF1A1 PE=1 SV=1)

HSP 1 Score: 370.933 bits (951), Expect = 3.734e-102
Identity = 186/248 (75.00%), Postives = 207/248 (83.47%), Query Frame = 1 
Query:   13 FVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGV 720
            FVPISG+ GDNM+E S N+ W+KG             TLL+ALD I  P RP+DKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTF P  +TTEVKSVEMHHEAL EALPGDNVGFNVKNV+VKD++RG VA +SK+DP  EAA FT+QVII+NHPGQI  GYAPVLDCHT+HIA KF+EL  KIDRRSGK+LE  PKFLK+GDA +V M+P KPM VE+FSDYPPLGRFAVRDMRQTVAVGV
Sbjct:  190 FVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGV 437
[Close]
BLAST of CX290139 vs. ExPASy Swiss-Prot
Match: EF1A1_PONAB (Elongation factor 1-alpha 1 OS=Pongo abelii GN=EEF1A1 PE=2 SV=2)

HSP 1 Score: 370.933 bits (951), Expect = 3.734e-102
Identity = 186/248 (75.00%), Postives = 207/248 (83.47%), Query Frame = 1 
Query:   13 FVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGV 720
            FVPISG+ GDNM+E S N+ W+KG             TLL+ALD I  P RP+DKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTF P  +TTEVKSVEMHHEAL EALPGDNVGFNVKNV+VKD++RG VA +SK+DP  EAA FT+QVII+NHPGQI  GYAPVLDCHT+HIA KF+EL  KIDRRSGK+LE  PKFLK+GDA +V M+P KPM VE+FSDYPPLGRFAVRDMRQTVAVGV
Sbjct:  190 FVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGV 437
[Close]
BLAST of CX290139 vs. ExPASy Swiss-Prot
Match: EF1A1_PANTR (Elongation factor 1-alpha 1 OS=Pan troglodytes GN=EEF1A1 PE=2 SV=1)

HSP 1 Score: 370.933 bits (951), Expect = 3.734e-102
Identity = 186/248 (75.00%), Postives = 207/248 (83.47%), Query Frame = 1 
Query:   13 FVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGV 720
            FVPISG+ GDNM+E S N+ W+KG             TLL+ALD I  P RP+DKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTF P  +TTEVKSVEMHHEAL EALPGDNVGFNVKNV+VKD++RG VA +SK+DP  EAA FT+QVII+NHPGQI  GYAPVLDCHT+HIA KF+EL  KIDRRSGK+LE  PKFLK+GDA +V M+P KPM VE+FSDYPPLGRFAVRDMRQTVAVGV
Sbjct:  190 FVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGV 437
[Close]
BLAST of CX290139 vs. ExPASy Swiss-Prot
Match: EF1A1_MOUSE (Elongation factor 1-alpha 1 OS=Mus musculus GN=Eef1a1 PE=1 SV=3)

HSP 1 Score: 370.933 bits (951), Expect = 3.734e-102
Identity = 186/248 (75.00%), Postives = 207/248 (83.47%), Query Frame = 1 
Query:   13 FVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGV 720
            FVPISG+ GDNM+E S N+ W+KG             TLL+ALD I  P RP+DKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTF P  +TTEVKSVEMHHEAL EALPGDNVGFNVKNV+VKD++RG VA +SK+DP  EAA FT+QVII+NHPGQI  GYAPVLDCHT+HIA KF+EL  KIDRRSGK+LE  PKFLK+GDA +V M+P KPM VE+FSDYPPLGRFAVRDMRQTVAVGV
Sbjct:  190 FVPISGWNGDNMLEPSANMPWFKGWKVTRKDGSASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGV 437
[Close]
BLAST of CX290139 vs. ExPASy Swiss-Prot
Match: EF1A1_HUMAN (Elongation factor 1-alpha 1 OS=Homo sapiens GN=EEF1A1 PE=1 SV=1)

HSP 1 Score: 370.933 bits (951), Expect = 3.734e-102
Identity = 186/248 (75.00%), Postives = 207/248 (83.47%), Query Frame = 1 
Query:   13 FVPISGFEGDNMIERSTNLDWYKG------------PTLLDALDNINEPKRPSDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFGPSGLTTEVKSVEMHHEALQEALPGDNVGFNVKNVAVKDLKRGFVASNSKDDPAREAANFTSQVIIMNHPGQIGNGYAPVLDCHTSHIAVKFSELVTKIDRRSGKELEKEPKFLKNGDAGMVKMIPTKPMVVETFSDYPPLGRFAVRDMRQTVAVGV 720
            FVPISG+ GDNM+E S N+ W+KG             TLL+ALD I  P RP+DKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTF P  +TTEVKSVEMHHEAL EALPGDNVGFNVKNV+VKD++RG VA +SK+DP  EAA FT+QVII+NHPGQI  GYAPVLDCHT+HIA KF+EL  KIDRRSGK+LE  PKFLK+GDA +V M+P KPM VE+FSDYPPLGRFAVRDMRQTVAVGV
Sbjct:  190 FVPISGWNGDNMLEPSANMPWFKGWKVTRKDGNASGTTLLEALDCILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPVNVTTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDVRRGNVAGDSKNDPPMEAAGFTAQVIILNHPGQISAGYAPVLDCHTAHIACKFAELKEKIDRRSGKKLEDGPKFLKSGDAAIVDMVPGKPMCVESFSDYPPLGRFAVRDMRQTVAVGV 437
The following BLAST results are available for this feature:
BLAST of CX290139 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
EF1A_CHICK2.189e-10275.40Elongation factor 1-alpha 1 OS=Gallus gallus GN=EE... [more]
EF1A_CAEEL2.189e-10271.89Elongation factor 1-alpha OS=Caenorhabditis elegan... [more]
EF1A2_RHIRA2.859e-10271.49Elongation factor 1-alpha OS=Rhizomucor racemosus ... [more]
EF1A1_RHIRA2.859e-10271.49Elongation factor 1-alpha OS=Rhizomucor racemosus ... [more]
EF1A1_RAT3.734e-10275.00Elongation factor 1-alpha 1 OS=Rattus norvegicus G... [more]
EF1A1_RABIT3.734e-10275.00Elongation factor 1-alpha 1 OS=Oryctolagus cunicul... [more]
EF1A1_PONAB3.734e-10275.00Elongation factor 1-alpha 1 OS=Pongo abelii GN=EEF... [more]
EF1A1_PANTR3.734e-10275.00Elongation factor 1-alpha 1 OS=Pan troglodytes GN=... [more]
EF1A1_MOUSE3.734e-10275.00Elongation factor 1-alpha 1 OS=Mus musculus GN=Eef... [more]
EF1A1_HUMAN3.734e-10275.00Elongation factor 1-alpha 1 OS=Homo sapiens GN=EEF... [more]

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Properties
Property NameValue
Genbank descriptionC02020H06SK IF1 Citrus clementina cDNA clone C02020H06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX290139 ID=CX290139; Name=CX290139; organism=Citrus clementina; type=EST; length=721bp
GCACGAGGCCCATTCGTGCCCATCTCTGGTTTCGAGGGTGACAACATGAT
TGAGAGGTCTACCAACCTTGACTGGTACAAGGGTCCAACCCTGCTGGACG
CTCTTGACAATATCAATGAGCCAAAGAGGCCCTCAGACAAGCCCCTTCGT
CTGCCACTCCAGGACGTGTACAAGATTGGTGGCATTGGAACCGTCCCAGT
GGGTCGCGTTGAGACCGGTGTCCTCAAGCCTGGTATGGTGGTGACATTTG
GCCCCTCTGGATTGACAACTGAGGTTAAGTCTGTTGAGATGCACCACGAA
GCTCTGCAGGAGGCCCTTCCGGGTGACAATGTTGGCTTCAACGTGAAGAA
CGTTGCTGTCAAGGATCTCAAGCGTGGTTTTGTTGCTTCCAACTCGAAGG
ATGATCCGGCCAGGGAAGCTGCCAACTTCACCTCACAGGTCATCATCATG
AACCACCCTGGCCAGATTGGAAATGGATATGCCCCAGTGCTTGACTGCCA
CACCTCCCACATTGCCGTCAAGTTTTCTGAGCTCGTGACGAAGATTGATA
GGCGATCAGGCAAGGAGCTTGAGAAGGAGCCCAAGTTCTTGAAGAATGGA
GATGCTGGAATGGTTAAGATGATTCCCACCAAGCCCATGGTTGTTGAAAC
CTTCTCTGACTACCCACCTCTTGGTCGTTTTGCCGTTAGGGACATGCGTC
AGACTGTTGCTGTTGGTGTGA
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