CX290254

Overview
NameCX290254
Unique NameCX290254
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length418
Libraries
Library NameType
IF1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_XYLF2 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Xylella fastidiosa (strain M23) GN=metE PE=3 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 7.386e-35
Identity = 76/129 (58.91%), Postives = 86/129 (66.67%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L GF FT  GWVQSYGSRCVKPPIIYGDV RP  MTV WS+ AQS+T +PMKGMLTGPVT+L WSFVR+DQ                             R +TC QIALA++DEV+DLEKAGI VIQ+
Sbjct:  503 LDGFIFTKLGWVQSYGSRCVKPPIIYGDVVRPAPMTVTWSAYAQSLTDKPMKGMLTGPVTMLQWSFVRDDQE----------------------------RAQTCRQIALALRDEVQDLEKAGIKVIQI 603
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_THIDA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Thiobacillus denitrificans (strain ATCC 25259) GN=metE PE=3 SV=1)

HSP 1 Score: 142.895 bits (359), Expect = 8.337e-35
Identity = 71/129 (55.04%), Postives = 86/129 (66.67%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L G+AF+  GWVQSYGSRCVKPPI++GDV RP+ MTV W+   QS+T +PMKGMLTGPVT+LNWSFVR+DQP                            R E+C Q+AL I+DEV DLEKAG+ VIQ+
Sbjct:  509 LDGYAFSRFGWVQSYGSRCVKPPILFGDVRRPRPMTVEWTRYGQSLTEKPMKGMLTGPVTLLNWSFVRDDQP----------------------------RAESCKQLALTIRDEVADLEKAGVRVIQI 609

HSP 2 Score: 25.409 bits (54), Expect = 8.337e-35
Identity = 11/11 (100.00%), Postives = 11/11 (100.00%), Query Frame = 1 
Query:  385 IDEAALREGLP 417
            IDEAALREGLP
Sbjct:  609 IDEAALREGLP 619
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_PELCD (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Pelobacter carbinolicus (strain DSM 2380 / Gra Bd 1) GN=metE PE=3 SV=1)

HSP 1 Score: 142.124 bits (357), Expect = 1.842e-34
Identity = 73/129 (56.59%), Postives = 85/129 (65.89%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L+GF FT NGWVQ YGSRCVKPPIIYGDV R K MT+ W++ AQ  T RP+KGMLTGPVT+L WSFVR+DQP                            R  TC QIALAI+DEV+DLE+AGI +IQ+
Sbjct:  500 LAGFCFTENGWVQGYGSRCVKPPIIYGDVERLKPMTMEWTTYAQQQTNRPLKGMLTGPVTMLCWSFVRDDQP----------------------------RSATCKQIALAIRDEVQDLEQAGIKIIQV 600

HSP 2 Score: 25.0238 bits (53), Expect = 1.842e-34
Identity = 10/11 (90.91%), Postives = 11/11 (100.00%), Query Frame = 1 
Query:  385 IDEAALREGLP 417
            +DEAALREGLP
Sbjct:  600 VDEAALREGLP 610
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_DICDI (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Dictyostelium discoideum GN=metE PE=3 SV=1)

HSP 1 Score: 143.665 bits (361), Expect = 2.807e-34
Identity = 73/129 (56.59%), Postives = 86/129 (66.67%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L+GF FT NGWVQSYGSRCVKPPIIYGDV+RP  MT+ +++ AQ++T +PMKGMLTGPVTIL WSFVR+DQP                            R ETC+QI LAI+DEV DLE  GI  IQ+
Sbjct:  565 LAGFVFTQNGWVQSYGSRCVKPPIIYGDVNRPVPMTLEYTTFAQTLTTKPMKGMLTGPVTILQWSFVRDDQP----------------------------RSETCFQIGLAIRDEVTDLENKGIACIQI 665
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_RUTMC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Ruthia magnifica subsp. Calyptogena magnifica GN=metE PE=3 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 4.071e-34
Identity = 70/129 (54.26%), Postives = 87/129 (67.44%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            LSG+ F+  GWVQSYGSRCVKPPII+GD+ RPK MT+ W + AQS+T +PMKGM+TGPVT+LNWSFVR+DQP                            R  TC Q++LAI+DEV DLEK+ IN+IQ+
Sbjct:  503 LSGYVFSQFGWVQSYGSRCVKPPIIFGDIFRPKPMTLDWITYAQSLTNKPMKGMITGPVTMLNWSFVRDDQP----------------------------RATTCLQLSLAIRDEVLDLEKSNINIIQI 603

HSP 2 Score: 25.409 bits (54), Expect = 4.071e-34
Identity = 11/11 (100.00%), Postives = 11/11 (100.00%), Query Frame = 1 
Query:  385 IDEAALREGLP 417
            IDEAALREGLP
Sbjct:  603 IDEAALREGLP 613
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_HAES1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Haemophilus somnus (strain 129Pt) GN=metE PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 6.253e-34
Identity = 76/129 (58.91%), Postives = 82/129 (63.57%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L GFAFT  GWVQSYGSRCVKPP+IYGDV RPKAMTV WS  AQS+T + MKGMLTGPVTIL WSFVRND P                            R   C QI +A+ DEV DLEKAGI VIQ+
Sbjct:  500 LDGFAFTKFGWVQSYGSRCVKPPVIYGDVVRPKAMTVRWSKYAQSLTKKVMKGMLTGPVTILQWSFVRNDIP----------------------------RSTVCKQIGVALSDEVLDLEKAGIKVIQI 600
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_CYTH3 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Cytophaga hutchinsonii (strain ATCC 33406 / NCIMB 9469) GN=metE PE=3 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 6.253e-34
Identity = 72/129 (55.81%), Postives = 85/129 (65.89%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L G+ F+ NGWVQSYGSRCVKPPIIYGD+ R + MTV W++LAQS+T + MKGMLTGPVTIL WSFVR+DQP                            R +TC QIA AI+DEV DLE AGI +IQ+
Sbjct:  515 LKGYVFSENGWVQSYGSRCVKPPIIYGDIERTEPMTVAWTTLAQSLTKKYMKGMLTGPVTILQWSFVRDDQP----------------------------RRDTCMQIAFAIRDEVVDLEAAGIKIIQI 615
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_ACIF5 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Acidithiobacillus ferrooxidans (strain ATCC 53993) GN=metE PE=3 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 1.067e-33
Identity = 75/129 (58.14%), Postives = 84/129 (65.12%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L+GFAFT +GWVQSYGSR VKPP+I+GDVSRP  MTV WS  AQS+T RPMKGMLTGPVTIL WSFVR+DQP                            R  T  QIALAI+DEV DL  AGI +IQ+
Sbjct:  527 LAGFAFTRHGWVQSYGSRYVKPPLIFGDVSRPTPMTVAWSKYAQSLTQRPMKGMLTGPVTILQWSFVRDDQP----------------------------RERTALQIALAIRDEVRDLIDAGIGIIQI 627
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_ACIF2 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Acidithiobacillus ferrooxidans (strain ATCC 23270 / DSM 14882 / NCIB 8455) GN=metE PE=3 SV=1)

HSP 1 Score: 141.739 bits (356), Expect = 1.067e-33
Identity = 75/129 (58.14%), Postives = 84/129 (65.12%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L+GFAFT +GWVQSYGSR VKPP+I+GDVSRP  MTV WS  AQS+T RPMKGMLTGPVTIL WSFVR+DQP                            R  T  QIALAI+DEV DL  AGI +IQ+
Sbjct:  527 LAGFAFTRHGWVQSYGSRYVKPPLIFGDVSRPTPMTVAWSKYAQSLTQRPMKGMLTGPVTILQWSFVRDDQP----------------------------RERTALQIALAIRDEVRDLIDAGIGIIQI 627
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_BRAJA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Bradyrhizobium japonicum GN=metE PE=3 SV=2)

HSP 1 Score: 139.043 bits (349), Expect = 1.171e-33
Identity = 71/129 (55.04%), Postives = 85/129 (65.89%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L+GFAFT NGWVQSYGSRCV+PPI++GDV RPK +TV W   AQS+T++PMK MLTGPVTILNWSFVR+D P                            R E C Q+ALA++DEV DLE AG  +IQ+
Sbjct:  520 LAGFAFTRNGWVQSYGSRCVRPPILFGDVVRPKPITVEWWRYAQSLTSKPMKAMLTGPVTILNWSFVRDDIP----------------------------RSEVCRQLALAMRDEVRDLENAGAAMIQI 620

HSP 2 Score: 25.409 bits (54), Expect = 1.171e-33
Identity = 11/11 (100.00%), Postives = 11/11 (100.00%), Query Frame = 1 
Query:  385 IDEAALREGLP 417
            IDEAALREGLP
Sbjct:  620 IDEAALREGLP 630
The following BLAST results are available for this feature:
BLAST of CX290254 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 269
Match NameE-valueIdentityDescription
METE_XYLF27.386e-3558.915-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_THIDA8.337e-3555.045-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_PELCD1.842e-3456.595-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_DICDI2.807e-3456.595-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RUTMC4.071e-3454.265-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_HAES16.253e-3458.915-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_CYTH36.253e-3455.815-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ACIF51.067e-3358.145-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ACIF21.067e-3358.145-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BRAJA1.171e-3355.045-methyltetrahydropteroyltriglutamate--homocystein... [more]

Pages

back to top
Properties
Property NameValue
Genbank descriptionC02022B12SK IF1 Citrus clementina cDNA clone C02022B12, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX290254 ID=CX290254; Name=CX290254; organism=Citrus clementina; type=EST; length=418bp
ATTATCAGGTTTCGCCTTCACTGCCAATGGGTGGGTGCAATCCTATGGAT
CTCGCTGTGTGAAGCCACCCATCATCTATGGTGATGTGAGCCGTCCCAAG
GCTATGACCGTCTTCTGGTCCTCCTTGGCTCAGAGCATGACTGCTCGCCC
AATGAAGGGAATGCTTACTGGCCCTGTCACCATTCTCAACTGGTCCTTTG
TCAGAAATGACCAGCCCAGGTATATAAATGAGAACTGGTTCACTATTGAT
TAACCTTTTGAAATGGAAACATTGTTCAACATAAATTGCTGCCAATTTTG
CAGACATGAAACCTGCTACCAGATTGCTTTGGCCATCAAGGACGAAGTCG
AGGATCTTGAGAAGGCAGGCATTAACGTTATTCAATTGATGAGGCTGCCT
TGAGGGAAGGATTGCCTC
back to top