CX290254

Overview
NameCX290254
Unique NameCX290254
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length418
Libraries
Library NameType
IF1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_HAEIE (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Haemophilus influenzae (strain PittEE) GN=metE PE=3 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 9.029e-33
Identity = 74/129 (57.36%), Postives = 81/129 (62.79%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L GFAFT  GWVQSYGSRCVKPP+IYGDV+RP+ MTV WS  AQS+T R MKGMLTGPVTIL WSFVRND P                            R   C QI +A+ DEV DLE AGI VIQ+
Sbjct:  499 LDGFAFTKFGWVQSYGSRCVKPPVIYGDVTRPEPMTVRWSQYAQSLTNRVMKGMLTGPVTILQWSFVRNDIP----------------------------RSTVCKQIGVALSDEVLDLEAAGIKVIQI 599
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_ACICJ (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Acidiphilium cryptum (strain JF-5) GN=metE PE=3 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 9.029e-33
Identity = 73/129 (56.59%), Postives = 88/129 (68.22%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L GFAFT +GWVQSYGSRCV PPII+GDV+RP+ MTV W+  AQS+TARP+KGMLTGPVT+L WSFVR+D PR               ET+             C QIALA++DEV DLE AG+ +IQ+
Sbjct:  507 LDGFAFTRHGWVQSYGSRCVAPPIIWGDVARPRPMTVRWARHAQSLTARPVKGMLTGPVTMLQWSFVRDDLPR---------------ETV-------------CRQIALALRDEVADLEAAGLAIIQV 607
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_NITOC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Nitrosococcus oceani (strain ATCC 19707 / NCIMB 11848) GN=metE PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 2.011e-32
Identity = 71/129 (55.04%), Postives = 84/129 (65.12%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L GFAFT NGWVQSYGSRCVKPPIIYGDV R + MTV W   AQS+T+R +KGMLTGPVT+L WSFVR+DQP                            R +TC Q+AL ++DEV DLE+A I +IQ+
Sbjct:  503 LEGFAFTQNGWVQSYGSRCVKPPIIYGDVVRRQPMTVAWLRYAQSLTSRRVKGMLTGPVTLLQWSFVRDDQP----------------------------RADTCLQLALVLRDEVRDLEQADIKLIQV 603
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_HAEIG (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Haemophilus influenzae (strain PittGG) GN=metE PE=3 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 2.011e-32
Identity = 74/129 (57.36%), Postives = 80/129 (62.02%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L GFAFT  GWVQSYGSRCVKPP+IYGDV RP+ MTV WS  AQS+T R MKGMLTGPVTIL WSFVRND P                            R   C QI +A+ DEV DLE AGI VIQ+
Sbjct:  499 LDGFAFTKFGWVQSYGSRCVKPPVIYGDVIRPEPMTVRWSQYAQSLTNRVMKGMLTGPVTILQWSFVRNDIP----------------------------RSTVCKQIGVALSDEVLDLEAAGIKVIQI 599
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_RALEU (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Ralstonia eutropha GN=metE PE=3 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 2.140e-32
Identity = 72/129 (55.81%), Postives = 84/129 (65.12%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L G+ FT NGWVQSYGSRCVKPP+IYGDV RP+ MTV  +  AQS+T RPMKGMLTGP+T+L WSFVR+DQP                            R  T  Q+ALAI+DEV DLE+AGI VIQ+
Sbjct:  499 LCGYGFTENGWVQSYGSRCVKPPVIYGDVYRPEPMTVDTARYAQSLTERPMKGMLTGPITMLQWSFVRDDQP----------------------------RATTARQLALAIRDEVCDLEQAGIRVIQI 599

HSP 2 Score: 23.483 bits (49), Expect = 2.140e-32
Identity = 10/11 (90.91%), Postives = 10/11 (90.91%), Query Frame = 1 
Query:  385 IDEAALREGLP 417
            IDE ALREGLP
Sbjct:  599 IDEPALREGLP 609
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_RALEH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Ralstonia eutropha (strain ATCC 17699 / H16 / DSM 428 / Stanier 337) GN=metE PE=3 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 2.140e-32
Identity = 72/129 (55.81%), Postives = 84/129 (65.12%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L G+ FT NGWVQSYGSRCVKPP+IYGDV RP+ MTV  +  AQS+T RPMKGMLTGP+T+L WSFVR+DQP                            R  T  Q+ALAI+DEV DLE+AGI VIQ+
Sbjct:  499 LCGYGFTENGWVQSYGSRCVKPPVIYGDVYRPEPMTVDTARYAQSLTERPMKGMLTGPITMLQWSFVRDDQP----------------------------RATTARQLALAIRDEVCDLEQAGIRVIQI 599

HSP 2 Score: 23.483 bits (49), Expect = 2.140e-32
Identity = 10/11 (90.91%), Postives = 10/11 (90.91%), Query Frame = 1 
Query:  385 IDEAALREGLP 417
            IDE ALREGLP
Sbjct:  599 IDEPALREGLP 609
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_SACD2 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Saccharophagus degradans (strain 2-40 / ATCC 43961 / DSM 17024) GN=metE PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 2.141e-32
Identity = 69/129 (53.49%), Postives = 85/129 (65.89%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L G+ FT  GWVQSYGSRCVKPPII+GDVSRP+ +TV W+  AQS+T++P+KGMLTGP+T+L WSFVR+DQPR I                            T  QIALA++ EV DLE AGI +IQ+
Sbjct:  508 LDGYVFTQFGWVQSYGSRCVKPPIIFGDVSRPRKITVDWAEYAQSLTSKPVKGMLTGPITMLQWSFVRDDQPRKI----------------------------TAQQIALALRSEVNDLEAAGITIIQV 608

HSP 2 Score: 23.0978 bits (48), Expect = 2.141e-32
Identity = 9/11 (81.82%), Postives = 10/11 (90.91%), Query Frame = 1 
Query:  385 IDEAALREGLP 417
            +DE ALREGLP
Sbjct:  608 VDEPALREGLP 618
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_BURXL (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia xenovorans (strain LB400) GN=metE PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 2.627e-32
Identity = 74/129 (57.36%), Postives = 84/129 (65.12%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L G+AFT NGWVQSYGSRCVKPPIIYGDV RP+ MTV  +  AQS+T R MKGMLTGPVT+L WSFVR+DQP                            R  T  Q+ALAI+DEV DLEKAGI +IQ+
Sbjct:  498 LWGYAFTENGWVQSYGSRCVKPPIIYGDVYRPEPMTVETTRYAQSLTQRLMKGMLTGPVTMLQWSFVRDDQP----------------------------RSTTALQLALAIRDEVVDLEKAGIRIIQI 598
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_BURPP (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia phytofirmans (strain DSM 17436 / PsJN) GN=metE PE=3 SV=1)

HSP 1 Score: 137.117 bits (344), Expect = 2.627e-32
Identity = 74/129 (57.36%), Postives = 84/129 (65.12%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L G+AFT NGWVQSYGSRCVKPPIIYGDV RP+ MTV  +  AQS+T R MKGMLTGPVT+L WSFVR+DQP                            R  T  Q+ALAI+DEV DLEKAGI +IQ+
Sbjct:  498 LWGYAFTENGWVQSYGSRCVKPPIIYGDVYRPEPMTVETTRYAQSLTQRLMKGMLTGPVTMLQWSFVRDDQP----------------------------RSTTALQLALAIRDEVVDLEKAGIRIIQI 598
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_YERPS (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Yersinia pseudotuberculosis GN=metE PE=3 SV=1)

HSP 1 Score: 136.346 bits (342), Expect = 2.788e-32
Identity = 72/129 (55.81%), Postives = 85/129 (65.89%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L GF FT NGWVQSYGSRCVKPP+I GD+SRP+A+TV W+  AQS+T +P+KGMLTGPVTIL WSF R D  R               ET+               QIALA++DEVEDLEKAGI +IQ+
Sbjct:  501 LDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAITVEWAKYAQSLTEKPVKGMLTGPVTILCWSFPREDVSR---------------ETI-------------AKQIALALRDEVEDLEKAGIGIIQI 601

HSP 2 Score: 23.483 bits (49), Expect = 2.788e-32
Identity = 10/11 (90.91%), Postives = 10/11 (90.91%), Query Frame = 1 
Query:  385 IDEAALREGLP 417
            IDE ALREGLP
Sbjct:  601 IDEPALREGLP 611
The following BLAST results are available for this feature:
BLAST of CX290254 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 269
Match NameE-valueIdentityDescription
METE_HAEIE9.029e-3357.365-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_ACICJ9.029e-3356.595-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_NITOC2.011e-3255.045-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_HAEIG2.011e-3257.365-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RALEU2.140e-3255.815-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RALEH2.140e-3255.815-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SACD22.141e-3253.495-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURXL2.627e-3257.365-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURPP2.627e-3257.365-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_YERPS2.788e-3255.815-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionC02022B12SK IF1 Citrus clementina cDNA clone C02022B12, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX290254 ID=CX290254; Name=CX290254; organism=Citrus clementina; type=EST; length=418bp
ATTATCAGGTTTCGCCTTCACTGCCAATGGGTGGGTGCAATCCTATGGAT
CTCGCTGTGTGAAGCCACCCATCATCTATGGTGATGTGAGCCGTCCCAAG
GCTATGACCGTCTTCTGGTCCTCCTTGGCTCAGAGCATGACTGCTCGCCC
AATGAAGGGAATGCTTACTGGCCCTGTCACCATTCTCAACTGGTCCTTTG
TCAGAAATGACCAGCCCAGGTATATAAATGAGAACTGGTTCACTATTGAT
TAACCTTTTGAAATGGAAACATTGTTCAACATAAATTGCTGCCAATTTTG
CAGACATGAAACCTGCTACCAGATTGCTTTGGCCATCAAGGACGAAGTCG
AGGATCTTGAGAAGGCAGGCATTAACGTTATTCAATTGATGAGGCTGCCT
TGAGGGAAGGATTGCCTC
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