CX290254

Overview
NameCX290254
Unique NameCX290254
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length418
Libraries
Library NameType
IF1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_SERP5 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Serratia proteamaculans (strain 568) GN=metE PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 4.728e-32
Identity = 70/129 (54.26%), Postives = 83/129 (64.34%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L GF FT NGWVQSYGSRCVKPP+I GD+SRP+A+TV W+  AQS+T +P+KGMLTGPVTIL WSF R D  R +                               QIALA++DEVEDLEKAGI +IQ+
Sbjct:  501 LDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPREDVTREV----------------------------IAKQIALALRDEVEDLEKAGIGIIQI 601

HSP 2 Score: 23.483 bits (49), Expect = 4.728e-32
Identity = 10/11 (90.91%), Postives = 10/11 (90.91%), Query Frame = 1 
Query:  385 IDEAALREGLP 417
            IDE ALREGLP
Sbjct:  601 IDEPALREGLP 611
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_MYCTU (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mycobacterium tuberculosis GN=metE PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 7.644e-32
Identity = 71/129 (55.04%), Postives = 85/129 (65.89%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L+GF  T NGWVQSYGSRCV+PPI+YGDVSRP+AMTV W + AQS+T +P+KGMLTGPVTIL WSFVR+DQP                              +T  Q+ALAI+DE  DL+ AGI VIQ+
Sbjct:  504 LAGFFATQNGWVQSYGSRCVRPPILYGDVSRPRAMTVEWITYAQSLTDKPVKGMLTGPVTILAWSFVRDDQP----------------------------LADTANQVALAIRDETVDLQSAGIAVIQV 604
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_MYCTA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mycobacterium tuberculosis (strain ATCC 25177 / H37Ra) GN=metE PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 7.644e-32
Identity = 71/129 (55.04%), Postives = 85/129 (65.89%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L+GF  T NGWVQSYGSRCV+PPI+YGDVSRP+AMTV W + AQS+T +P+KGMLTGPVTIL WSFVR+DQP                              +T  Q+ALAI+DE  DL+ AGI VIQ+
Sbjct:  504 LAGFFATQNGWVQSYGSRCVRPPILYGDVSRPRAMTVEWITYAQSLTDKPVKGMLTGPVTILAWSFVRDDQP----------------------------LADTANQVALAIRDETVDLQSAGIAVIQV 604
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_MYCBP (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mycobacterium bovis (strain BCG / Pasteur 1173P2) GN=metE PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 7.644e-32
Identity = 71/129 (55.04%), Postives = 85/129 (65.89%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L+GF  T NGWVQSYGSRCV+PPI+YGDVSRP+AMTV W + AQS+T +P+KGMLTGPVTIL WSFVR+DQP                              +T  Q+ALAI+DE  DL+ AGI VIQ+
Sbjct:  504 LAGFFATQNGWVQSYGSRCVRPPILYGDVSRPRAMTVEWITYAQSLTDKPVKGMLTGPVTILAWSFVRDDQP----------------------------LADTANQVALAIRDETVDLQSAGIAVIQV 604
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_MYCBO (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mycobacterium bovis GN=metE PE=3 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 7.644e-32
Identity = 71/129 (55.04%), Postives = 85/129 (65.89%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L+GF  T NGWVQSYGSRCV+PPI+YGDVSRP+AMTV W + AQS+T +P+KGMLTGPVTIL WSFVR+DQP                              +T  Q+ALAI+DE  DL+ AGI VIQ+
Sbjct:  504 LAGFFATQNGWVQSYGSRCVRPPILYGDVSRPRAMTVEWITYAQSLTDKPVKGMLTGPVTILAWSFVRDDQP----------------------------LADTANQVALAIRDETVDLQSAGIAVIQV 604
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_BURTA (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia thailandensis (strain E264 / ATCC 700388 / DSM 13276 / CIP 106301) GN=metE PE=3 SV=1)

HSP 1 Score: 135.191 bits (339), Expect = 8.017e-32
Identity = 69/127 (54.33%), Postives = 83/127 (65.35%), Query Frame = 2 
Query:    8 GFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            GFA T+NGWVQSYGSRCVKPP++YGDV  P+ MTV W+S AQS+T +P+KGMLTGPVT+L WSFVR+DQP                            R  T  QIALA++ E  DLEKAGI +IQ+
Sbjct:  504 GFAITSNGWVQSYGSRCVKPPLVYGDVYLPEPMTVGWASYAQSLTTKPVKGMLTGPVTMLQWSFVRDDQP----------------------------RATTALQIALALRQETLDLEKAGIGMIQV 602

HSP 2 Score: 23.0978 bits (48), Expect = 8.017e-32
Identity = 9/11 (81.82%), Postives = 10/11 (90.91%), Query Frame = 1 
Query:  385 IDEAALREGLP 417
            +DE ALREGLP
Sbjct:  602 VDEPALREGLP 612
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_RHILO (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Rhizobium loti GN=metE PE=3 SV=1)

HSP 1 Score: 132.109 bits (331), Expect = 1.359e-31
Identity = 70/129 (54.26%), Postives = 81/129 (62.79%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L GFAFT +GWVQSYGSR V+PPII GDVSR   MT+ W   AQS+T +P+KGMLTGPVTILNWSFVR+D P                            R   C Q+ALAI+DEV DLEKAG  +IQ+
Sbjct:  517 LRGFAFTQHGWVQSYGSRFVRPPIIIGDVSRQNPMTLHWWRYAQSLTPKPVKGMLTGPVTILNWSFVRDDLP----------------------------RSAVCRQVALAIRDEVSDLEKAGAKMIQI 617

HSP 2 Score: 25.409 bits (54), Expect = 1.359e-31
Identity = 11/11 (100.00%), Postives = 11/11 (100.00%), Query Frame = 1 
Query:  385 IDEAALREGLP 417
            IDEAALREGLP
Sbjct:  617 IDEAALREGLP 627
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_SHEON (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Shewanella oneidensis GN=metE PE=3 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 1.703e-31
Identity = 70/129 (54.26%), Postives = 81/129 (62.79%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L G  FT NGWVQSYGSRCVKPP+IYGDVSRPKAMTV W+  AQS+T +P+KGMLTGPVTIL+WSF R D                              R     Q+ALAI+DEV DL+ AGI +IQ+
Sbjct:  503 LEGVGFTKNGWVQSYGSRCVKPPLIYGDVSRPKAMTVDWAVFAQSLTDKPVKGMLTGPVTILHWSFARED----------------------------ISRDTIATQLALAIRDEVVDLQHAGIGIIQI 603
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_SODGM (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Sodalis glossinidius (strain morsitans) GN=metE PE=3 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 2.306e-31
Identity = 70/129 (54.26%), Postives = 82/129 (63.57%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L GF FT NGWVQSYGSRCVKPPII GD+SRP+A+T+ W+  AQS+TA+P+KGMLTGPVTIL WSF R D                              R     QIALA++DEV DLE+AGI VIQ+
Sbjct:  501 LDGFVFTQNGWVQSYGSRCVKPPIIIGDISRPEAITIEWARYAQSLTAKPVKGMLTGPVTILCWSFPRED----------------------------VSRKTIARQIALALRDEVADLEQAGIGVIQI 601

HSP 2 Score: 23.483 bits (49), Expect = 2.306e-31
Identity = 10/11 (90.91%), Postives = 10/11 (90.91%), Query Frame = 1 
Query:  385 IDEAALREGLP 417
            IDE ALREGLP
Sbjct:  601 IDEPALREGLP 611
[Close]
BLAST of CX290254 vs. ExPASy Swiss-Prot
Match: METE_KLEP7 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Klebsiella pneumoniae subsp. pneumoniae (strain ATCC 700721 / MGH 78578) GN=metE PE=3 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 2.306e-31
Identity = 71/129 (55.04%), Postives = 84/129 (65.12%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNWSFVRNDQPRYINENWFTID*PFEMETLFNINCCQFCRHETCYQIALAIKDEVEDLEKAGINVIQL 388
            L GF FT NGWVQSYGSRCVKPP++ GDVSRP+A+TV W+  AQS+TA+P+KGMLTGPVTIL WSF R D  R               ET+               QIALA++DEV DLE AGI +IQ+
Sbjct:  498 LDGFIFTQNGWVQSYGSRCVKPPVVIGDVSRPQAITVDWAKYAQSLTAKPVKGMLTGPVTILCWSFPREDVSR---------------ETI-------------AKQIALALRDEVADLEAAGIGIIQI 598

HSP 2 Score: 23.483 bits (49), Expect = 2.306e-31
Identity = 10/11 (90.91%), Postives = 10/11 (90.91%), Query Frame = 1 
Query:  385 IDEAALREGLP 417
            IDE ALREGLP
Sbjct:  598 IDEPALREGLP 608
The following BLAST results are available for this feature:
BLAST of CX290254 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 269
Match NameE-valueIdentityDescription
METE_SERP54.728e-3254.265-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_MYCTU7.644e-3255.045-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_MYCTA7.644e-3255.045-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_MYCBP7.644e-3255.045-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_MYCBO7.644e-3255.045-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURTA8.017e-3254.335-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_RHILO1.359e-3154.265-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SHEON1.703e-3154.265-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SODGM2.306e-3154.265-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_KLEP72.306e-3155.045-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionC02022B12SK IF1 Citrus clementina cDNA clone C02022B12, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX290254 ID=CX290254; Name=CX290254; organism=Citrus clementina; type=EST; length=418bp
ATTATCAGGTTTCGCCTTCACTGCCAATGGGTGGGTGCAATCCTATGGAT
CTCGCTGTGTGAAGCCACCCATCATCTATGGTGATGTGAGCCGTCCCAAG
GCTATGACCGTCTTCTGGTCCTCCTTGGCTCAGAGCATGACTGCTCGCCC
AATGAAGGGAATGCTTACTGGCCCTGTCACCATTCTCAACTGGTCCTTTG
TCAGAAATGACCAGCCCAGGTATATAAATGAGAACTGGTTCACTATTGAT
TAACCTTTTGAAATGGAAACATTGTTCAACATAAATTGCTGCCAATTTTG
CAGACATGAAACCTGCTACCAGATTGCTTTGGCCATCAAGGACGAAGTCG
AGGATCTTGAGAAGGCAGGCATTAACGTTATTCAATTGATGAGGCTGCCT
TGAGGGAAGGATTGCCTC
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