CX294957

Overview
NameCX294957
Unique NameCX294957
TypeEST
OrganismCitrus clementina (Clementine)
Sequence length587
Libraries
Library NameType
DroRLeaf1cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CX294957_ssr209 microsatellite CX294957_ssr209:209..220. BLAST: Citrus ESTs to Prunus persica proteins V1
Ccv1_Contig5796 contig Ccv1_Contig5796:63..650. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P_MYCTU (Glyceraldehyde-3-phosphate dehydrogenase OS=Mycobacterium tuberculosis GN=gap PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 8.867e-14
Identity = 41/92 (44.57%), Postives = 53/92 (57.61%), Query Frame = 2 
Query:  251 LKVAINGFGRIGRNFLRCWHGRKD---SPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNR-NPVNLPWGD 514
            ++V INGFGRIGRNF R    +++   + +EVVA ND       +HLLK+DS LG    DV   G D I V    I+ ++ R  P  LPWGD
Sbjct:    3 VRVGINGFGRIGRNFYRALLAQQEQGTADVEVVAANDITDNSTLAHLLKFDSILGRLPCDVGLEGDDTIVVGRAKIKALAVREGPAALPWGD 94

HSP 2 Score: 29.261 bits (64), Expect = 8.867e-14
Identity = 11/23 (47.83%), Postives = 16/23 (69.57%), Query Frame = 1 
Query:  490 PRQSPLGGLGIDLVIEGTGVFVD 558
            P   P G LG+D+V+E TG+F +
Sbjct:   87 PAALPWGDLGVDVVVESTGLFTN 109
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P_MYCBO (Glyceraldehyde-3-phosphate dehydrogenase OS=Mycobacterium bovis GN=gap PE=3 SV=1)

HSP 1 Score: 67.781 bits (164), Expect = 8.867e-14
Identity = 41/92 (44.57%), Postives = 53/92 (57.61%), Query Frame = 2 
Query:  251 LKVAINGFGRIGRNFLRCWHGRKD---SPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNR-NPVNLPWGD 514
            ++V INGFGRIGRNF R    +++   + +EVVA ND       +HLLK+DS LG    DV   G D I V    I+ ++ R  P  LPWGD
Sbjct:    3 VRVGINGFGRIGRNFYRALLAQQEQGTADVEVVAANDITDNSTLAHLLKFDSILGRLPCDVGLEGDDTIVVGRAKIKALAVREGPAALPWGD 94

HSP 2 Score: 29.261 bits (64), Expect = 8.867e-14
Identity = 11/23 (47.83%), Postives = 16/23 (69.57%), Query Frame = 1 
Query:  490 PRQSPLGGLGIDLVIEGTGVFVD 558
            P   P G LG+D+V+E TG+F +
Sbjct:   87 PAALPWGDLGVDVVVESTGLFTN 109
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P_MYCPN (Glyceraldehyde-3-phosphate dehydrogenase OS=Mycoplasma pneumoniae GN=gapA PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 1.152e-13
Identity = 40/95 (42.11%), Postives = 56/95 (58.95%), Query Frame = 2 
Query:  233 VAAQAKLKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDG-ISVDGKVIQVVSNRNPVNLPWGD 514
            +A    ++VAINGFGRIGR   R    +K+  +E+VA+ND       +HLLKYDS  G F+   K V  D  + +D K + V S ++P NLPW +
Sbjct:    2 LAKSKTIRVAINGFGRIGRLVFRALLSQKN--IEIVAVNDLTHPDTLAHLLKYDSAHGEFKK--KVVAKDNTLMIDKKKVLVFSEKDPANLPWAE 92

HSP 2 Score: 28.1054 bits (61), Expect = 1.152e-13
Identity = 13/28 (46.43%), Postives = 15/28 (53.57%), Query Frame = 1 
Query:  490 PRQSPLGGLGIDLVIEGTGVFVDREGGS 573
            P   P     ID+V+E TG FV  EG S
Sbjct:   85 PANLPWAEHNIDIVVESTGRFVSEEGAS 112
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P_STRAU (Glyceraldehyde-3-phosphate dehydrogenase OS=Streptomyces aureofaciens GN=gap PE=3 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 1.153e-13
Identity = 42/89 (47.19%), Postives = 54/89 (60.67%), Query Frame = 2 
Query:  254 KVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDG--ISVDGKVIQVVSNRNPVNLPWGD 514
            ++AINGFGRIGRN LR    R DS L+VVA+ND       + LL YD+T G      +PV  +G  + VDG+ I V + R P NLPW +
Sbjct:    3 RIAINGFGRIGRNVLRALLER-DSDLDVVAVNDLTEPATLARLLAYDTTSGRLG---RPVTVEGNVLVVDGRRITVTAEREPANLPWAE 87

HSP 2 Score: 25.7942 bits (55), Expect = 1.153e-13
Identity = 10/22 (45.45%), Postives = 14/22 (63.64%), Query Frame = 1 
Query:  487 QPRQSPLGGLGIDLVIEGTGVF 552
            +P   P   LG+D+V+E TG F
Sbjct:   79 EPANLPWAELGVDIVLEATGRF 100
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: E4PD_TOLAT (D-erythrose-4-phosphate dehydrogenase OS=Tolumonas auensis (strain DSM 9187 / TA4) GN=epd PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.497e-13
Identity = 40/90 (44.44%), Postives = 57/90 (63.33%), Query Frame = 2 
Query:  251 LKVAINGFGRIGRNFLRCWH--GRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNRNPVNLPWGD 514
            +++AINGFGRIGRN LR  +  GR+ + ++VVAIN+       +HLLKYDST G F+ DV+    D + V    I+++  +    LPWGD
Sbjct:    3 IRIAINGFGRIGRNVLRALYESGRR-AEIKVVAINEPADAAGMAHLLKYDSTHGRFDWDVRQ-ERDVLYVGDDAIRLLHQKEISQLPWGD 90

HSP 2 Score: 26.9498 bits (58), Expect = 1.497e-13
Identity = 11/25 (44.00%), Postives = 17/25 (68.00%), Query Frame = 1 
Query:  496 QSPLGGLGIDLVIEGTGVFVDREGG 570
            Q P G LG+D+V++ +GV+  R  G
Sbjct:   85 QLPWGDLGVDIVLDCSGVYGKRADG 109
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P_ASHGO (Glyceraldehyde-3-phosphate dehydrogenase OS=Ashbya gossypii GN=GPD PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 1.945e-13
Identity = 41/89 (46.07%), Postives = 56/89 (62.92%), Query Frame = 2 
Query:  251 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTG-GVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNRNPVNLPWGD 514
            +KVAING G+IGR  +R    R +  +EVVAIND    V  A+++ KYDST G +  DV+  G   + +DGK I+V   R+P  LPWG+
Sbjct:    2 VKVAINGLGKIGRLVMRIALSRAN--VEVVAINDPFITVDYAAYMFKYDSTHGKYAGDVQYEGNT-LVIDGKKIKVFQERDPAQLPWGE 87

HSP 2 Score: 27.7202 bits (60), Expect = 1.945e-13
Identity = 12/21 (57.14%), Postives = 14/21 (66.67%), Query Frame = 1 
Query:  490 PRQSPLGGLGIDLVIEGTGVF 552
            P Q P G  GID+ I+ TGVF
Sbjct:   80 PAQLPWGEEGIDIAIDSTGVF 100
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P_CORGL (Glyceraldehyde-3-phosphate dehydrogenase OS=Corynebacterium glutamicum GN=gap PE=1 SV=2)

HSP 1 Score: 72.7886 bits (177), Expect = 2.526e-13
Identity = 43/86 (50.00%), Postives = 53/86 (61.63%), Query Frame = 2 
Query:  251 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNRNPVNLPW 508
            ++V INGFGRIGRNF R    R D  LEVVA+ND    K  S LLK+DS +G    +V+    D I+V GK I V + R+P NL W
Sbjct:    3 IRVGINGFGRIGRNFFRAVLERSDD-LEVVAVNDLTDNKTLSTLLKFDSIMGRLGQEVE-YDDDSITVGGKRIAVYAERDPKNLDW 86

HSP 2 Score: 22.7126 bits (47), Expect = 2.526e-13
Identity = 9/23 (39.13%), Postives = 12/23 (52.17%), Query Frame = 1 
Query:  490 PRQSPLGGLGIDLVIEGTGVFVD 558
            P+        +D+VIE TG F D
Sbjct:   81 PKNLDWAAHNVDIVIESTGFFTD 103
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P_BUCAI (Glyceraldehyde-3-phosphate dehydrogenase OS=Buchnera aphidicola subsp. Acyrthosiphon pisum GN=gapA PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 2.527e-13
Identity = 38/88 (43.18%), Postives = 55/88 (62.50%), Query Frame = 2 
Query:  251 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNRNPVNLPWGD 514
            +KV INGFGRIGR   R    +K S +E++AIND    +  +++LKYDST G+F+  +     D I ++GK I+V S ++P  L W D
Sbjct:    3 IKVGINGFGRIGRVLFRL--AQKRSDIEIIAINDLLAPEYIAYMLKYDSTHGVFKKHI-TFDKDNIIINGKNIRVTSIKDPEKLMWHD 87

HSP 2 Score: 26.1794 bits (56), Expect = 2.527e-13
Identity = 11/17 (64.71%), Postives = 14/17 (82.35%), Query Frame = 1 
Query:  514 LGIDLVIEGTGVFVDRE 564
            L ID+VIE TG+F+ RE
Sbjct:   88 LSIDVVIESTGLFLKRE 104
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: G3P1_CANGA (Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Candida glabrata GN=GPD1 PE=3 SV=1)

HSP 1 Score: 68.5514 bits (166), Expect = 2.527e-13
Identity = 41/89 (46.07%), Postives = 55/89 (61.80%), Query Frame = 2 
Query:  251 LKVAINGFGRIGRNFLRCWHGRKDSPLEVVAINDTGGVKQ-ASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNRNPVNLPWGD 514
            ++VAINGFGRIGR  LR     K   ++VVAIND       A+++ KYDST G ++ +V     D I +DGK I+    R+P +LPWGD
Sbjct:    2 VRVAINGFGRIGRIVLRI--AMKRDGIDVVAINDPFITNDYAAYMFKYDSTHGRYDGEVTHDDKDLI-IDGKAIKCYQERDPADLPWGD 87

HSP 2 Score: 26.9498 bits (58), Expect = 2.527e-13
Identity = 12/25 (48.00%), Postives = 15/25 (60.00%), Query Frame = 1 
Query:  490 PRQSPLGGLGIDLVIEGTGVFVDRE 564
            P   P G   ID+VIE TGVF  ++
Sbjct:   80 PADLPWGDNDIDIVIEATGVFTAKD 104
[Close]
BLAST of CX294957 vs. ExPASy Swiss-Prot
Match: E4PD_PROMH (D-erythrose-4-phosphate dehydrogenase OS=Proteus mirabilis (strain HI4320) GN=epd PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 3.279e-13
Identity = 38/89 (42.70%), Postives = 55/89 (61.80%), Query Frame = 2 
Query:  251 LKVAINGFGRIGRNFLRC-WHGRKDSPLEVVAINDTGGVKQASHLLKYDSTLGIFEADVKPVGTDGISVDGKVIQVVSNRNPVNLPWGD 514
            ++VAINGFGRIGRN LR  +   K + + VVAIN+       +HLLKYDS+ G F  D++ V  D + V    I++  + +  +LPWG+
Sbjct:    3 IRVAINGFGRIGRNVLRALYESGKRAEISVVAINELADATGIAHLLKYDSSHGRFAWDIR-VNNDLLQVGDDAIRLFHHADIADLPWGE 90

HSP 2 Score: 25.7942 bits (55), Expect = 3.279e-13
Identity = 11/23 (47.83%), Postives = 16/23 (69.57%), Query Frame = 1 
Query:  502 PLGGLGIDLVIEGTGVFVDREGG 570
            P G LGID+V++ +GV+  R  G
Sbjct:   87 PWGELGIDVVLDCSGVYGSRADG 109
The following BLAST results are available for this feature:
BLAST of CX294957 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 114
Match NameE-valueIdentityDescription
G3P_MYCTU8.867e-1444.57Glyceraldehyde-3-phosphate dehydrogenase OS=Mycoba... [more]
G3P_MYCBO8.867e-1444.57Glyceraldehyde-3-phosphate dehydrogenase OS=Mycoba... [more]
G3P_MYCPN1.152e-1342.11Glyceraldehyde-3-phosphate dehydrogenase OS=Mycopl... [more]
G3P_STRAU1.153e-1347.19Glyceraldehyde-3-phosphate dehydrogenase OS=Strept... [more]
E4PD_TOLAT1.497e-1344.44D-erythrose-4-phosphate dehydrogenase OS=Tolumonas... [more]
G3P_ASHGO1.945e-1346.07Glyceraldehyde-3-phosphate dehydrogenase OS=Ashbya... [more]
G3P_CORGL2.526e-1350.00Glyceraldehyde-3-phosphate dehydrogenase OS=Coryne... [more]
G3P_BUCAI2.527e-1343.18Glyceraldehyde-3-phosphate dehydrogenase OS=Buchne... [more]
G3P1_CANGA2.527e-1346.07Glyceraldehyde-3-phosphate dehydrogenase 1 OS=Cand... [more]
E4PD_PROMH3.279e-1342.70D-erythrose-4-phosphate dehydrogenase OS=Proteus m... [more]

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Properties
Property NameValue
Genbank descriptionC05065A08SK DroRLeaf1 Citrus clementina cDNA clone C05065A08, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX294957 ID=CX294957; Name=CX294957; organism=Citrus clementina; type=EST; length=587bp
GAGGCCACTGCTTGCTCTTGCTTGCATCATCCAGCAAATACCTATCATGG
CTTCGGCCACTCTCTCTGTAGCCAAATCAGCTCTTCAGGGAAATGGTAAG
GGATTCTCAGAATTCTCAGGTCTACGCAACTCTGCAAGCCTTCCTTTTGG
CAGGAAATCTTCTGATGACTTCCATTCAGTCATTGCTCTCCAGACCTCTG
CTTTAGGAAGCAGCAGCAGCGGGTACAGGAAGGTTGCTGCTCAGGCAAAG
CTGAAGGTTGCAATCAATGGGTTTGGTAGAATTGGTAGGAACTTCTTGAG
GTGCTGGCACGGCCGCAAGGACTCTCCACTTGAAGTCGTTGCCATCAATG
ACACCGGAGGTGTCAAGCAGGCCTCTCACCTCCTCAAGTACGACTCCACC
CTTGGCATCTTTGAAGCCGATGTTAAACCAGTTGGTACTGATGGCATCTC
TGTTGATGGCAAGGTCATCCAGGTTGTTTCTAACCGCAACCCCGTCAATC
TCCCCTGGGGGGACTGGGCATCGACCTTGTGATTGAAGGGACAGGAGTGT
TTGTAGACAGAGAGGGCGGCAGCACATCAGCTGTGCA
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